India suffers post-harvest losses of over $2 billion annually due to spoilage of 30% of fruits and vegetables after harvesting. Elicitors are compounds that can activate natural host defense responses in plants. Some examples of elicitors that have been shown to reduce post-harvest diseases include salicylic acid, methyl jasmonate, harpin, and oligandrin. Elicitors work by priming the plant's defenses through mechanisms like reactive oxygen species production, phytoalexin production, and hypersensitive response. The effectiveness of elicitors can depend on factors like cultivar genotype, environmental conditions, and whether they are combined with other treatments like fungicides or biocontrol agents.
Molecular basis of plant resistance and defense responses to pathogensSenthil Natesan
Â
In response to pathogen attack, plants have evolved sophisticated defense mechanisms to delay or arrest pathogen growth.Unlike animals, plants lack a circulating immune system recognizing microbial pathogens. Plant cells are more autonomous in their defense mechanisms and rely on the innate immune capacity of each cell and systemic signals that disseminate from infection sites (Jones and Dangl, 2006). Plant innate immunity consists of preformed physical and chemical barriers (such as leaf hairs, rigid cell walls, pre-existing antimicrobial compounds) and induced defenses. Should an invading microbe successfully breach the pre-formed barriers, it may be recognized by the plant, resulting in the activation of cellular defense responses that stop or restrict further development of the invader.
The concept of gene for gene hypothesis was first developed by Flor in 1956 based on his studies of host pathogen interaction in flax, for rust caused by Melampsora lini. The gene for gene hypothesis states that for each gene controlling resistance in the host, there is corresponding gene controlling pathogenicity in the pathogen. The resistance of host is governed by dominant genes and virulence of pathogen by recessive genes. The genotype of host and pathogen determine the disease reaction. When genes in host and pathogen match for all loci, then only the host will show susceptible reaction. If some gene loci remain unmatched, the host will show resistant reaction. Now gene â for âgene relationship has been reported in several other crops like potato, sorghum, wheat, etc. The gene for gene hypothesis is also known as âFlor Hypothesis.â
plant pathogen interaction
different types of pathogens
gene for gene hypothesis
direct receptor model
Elicitor receptor model
suppersor repressor model
gaurd hypothesis
Molecular basis of plant resistance and defense responses to pathogensSenthil Natesan
Â
In response to pathogen attack, plants have evolved sophisticated defense mechanisms to delay or arrest pathogen growth.Unlike animals, plants lack a circulating immune system recognizing microbial pathogens. Plant cells are more autonomous in their defense mechanisms and rely on the innate immune capacity of each cell and systemic signals that disseminate from infection sites (Jones and Dangl, 2006). Plant innate immunity consists of preformed physical and chemical barriers (such as leaf hairs, rigid cell walls, pre-existing antimicrobial compounds) and induced defenses. Should an invading microbe successfully breach the pre-formed barriers, it may be recognized by the plant, resulting in the activation of cellular defense responses that stop or restrict further development of the invader.
The concept of gene for gene hypothesis was first developed by Flor in 1956 based on his studies of host pathogen interaction in flax, for rust caused by Melampsora lini. The gene for gene hypothesis states that for each gene controlling resistance in the host, there is corresponding gene controlling pathogenicity in the pathogen. The resistance of host is governed by dominant genes and virulence of pathogen by recessive genes. The genotype of host and pathogen determine the disease reaction. When genes in host and pathogen match for all loci, then only the host will show susceptible reaction. If some gene loci remain unmatched, the host will show resistant reaction. Now gene â for âgene relationship has been reported in several other crops like potato, sorghum, wheat, etc. The gene for gene hypothesis is also known as âFlor Hypothesis.â
plant pathogen interaction
different types of pathogens
gene for gene hypothesis
direct receptor model
Elicitor receptor model
suppersor repressor model
gaurd hypothesis
Plants have array of defense response against biotic stresses which could be either structural reinforcement, release of chemicals, and defense gene expression against invading organisms. The physical barriers are trichoms, waxy cuticle, thick cell wall. Once the pathogen overcomes the first line of defense, basal or innate defense response comes into play. Pathogens secrete some conserved molecules known as Pathogen Associated Molecular Pattern (PAMP/MAMP), which are recognized by transmembrane receptors present in the plasma membrane and initiate a series of signal cascade reaction which ultimately leads to activation of various defense related genes. Apart from inducing the expression of defense related genes, it also triggers a hypersensitive reaction (HR) which cause deliberate cell death at the site of infection and limit the pathogen access to water and nutrient by sacrificing a few cells in order to save the rest of the plant. Once HR is triggered, plant tissue may become highly resistant to a broad range of pathogens for an extended period of time. This phenomenon is called Systemic Acquired Resistance (SAR).
Plants respond to herbivory is a similar manner as described above. The biochemical mechanisms of defense against the herbivores are wide-ranging, highly dynamic, and are mediated both by direct and indirect defenses. The defensive compounds are either produced constitutively or in response to plant damage, and affect feeding, growth, and survival of herbivores. In addition, plants also release volatile organic compounds that attract the natural enemies of the herbivores. These strategies either act independently or in conjunction with each other. However, our understanding of these defensive mechanisms is still limited. Induced resistance could be exploited as an important tool for the pest management to minimize the amounts of insecticides used for pest control. Host plant resistance to insects, particularly, induced resistance, can also be manipulated with the use of chemical elicitors of secondary metabolites, which confer resistance to insects. By understanding the mechanisms of induced resistance, we can predict the herbivores that are likely to be affected by inducing responses. The elicitors of induced responses can be sprayed on crop plants to build up the natural defense system against damage caused by herbivores. The induced responses can also be genetically engineered, so that the defensive compounds are constitutively produced in plants challenged by the herbivory. Induced resistance can be exploited for developing crop cultivars, which readily produce the inducible response upon mild infestation, and can act as one of components of integrated pest management for sustainable crop production.
Plant Disease Resistant And Genetic EngineeringShweta Jhakhar
Â
Study the adverse effects of different viruses and other fungal diseases on the plants and their growth. Discuss the methods e.g. plant disease resistant and genetic engineering to protect the plants.
Plants have array of defense response against biotic stresses which could be either structural reinforcement, release of chemicals, and defense gene expression against invading organisms. The physical barriers are trichoms, waxy cuticle, thick cell wall. Once the pathogen overcomes the first line of defense, basal or innate defense response comes into play. Pathogens secrete some conserved molecules known as Pathogen Associated Molecular Pattern (PAMP/MAMP), which are recognized by transmembrane receptors present in the plasma membrane and initiate a series of signal cascade reaction which ultimately leads to activation of various defense related genes. Apart from inducing the expression of defense related genes, it also triggers a hypersensitive reaction (HR) which cause deliberate cell death at the site of infection and limit the pathogen access to water and nutrient by sacrificing a few cells in order to save the rest of the plant. Once HR is triggered, plant tissue may become highly resistant to a broad range of pathogens for an extended period of time. This phenomenon is called Systemic Acquired Resistance (SAR).
Plants respond to herbivory is a similar manner as described above. The biochemical mechanisms of defense against the herbivores are wide-ranging, highly dynamic, and are mediated both by direct and indirect defenses. The defensive compounds are either produced constitutively or in response to plant damage, and affect feeding, growth, and survival of herbivores. In addition, plants also release volatile organic compounds that attract the natural enemies of the herbivores. These strategies either act independently or in conjunction with each other. However, our understanding of these defensive mechanisms is still limited. Induced resistance could be exploited as an important tool for the pest management to minimize the amounts of insecticides used for pest control. Host plant resistance to insects, particularly, induced resistance, can also be manipulated with the use of chemical elicitors of secondary metabolites, which confer resistance to insects. By understanding the mechanisms of induced resistance, we can predict the herbivores that are likely to be affected by inducing responses. The elicitors of induced responses can be sprayed on crop plants to build up the natural defense system against damage caused by herbivores. The induced responses can also be genetically engineered, so that the defensive compounds are constitutively produced in plants challenged by the herbivory. Induced resistance can be exploited for developing crop cultivars, which readily produce the inducible response upon mild infestation, and can act as one of components of integrated pest management for sustainable crop production.
Plant Disease Resistant And Genetic EngineeringShweta Jhakhar
Â
Study the adverse effects of different viruses and other fungal diseases on the plants and their growth. Discuss the methods e.g. plant disease resistant and genetic engineering to protect the plants.
An overview on role of signal transduction in inducing plant innate immunity which includes both systemic acquired resistance as well as induced systemic resistance.
Management of host plant resistance through immunizationAnshul Arya
Â
it is a small presentation prepared for seminar purpose .immunization is a new technique very few people know about it even i did not get any slide prepared by it earlier even whatever i got was not purchased .so i prepared it for those who are interested to know about it without having problems to find the matter for it.
Content:
Introduction
Importance of Host Plant Resistance
Historical perspectives
Advantages and Disadvantages of HPR
Mechanisms of Resistance
Adaptation of Resistance in Plant to Insect
Morphological
Anatomical
Biochemical
Assembly of plant species - Gene Pool
Behavior in Relation to Host Plant Factor
â The diseases caused by bipartite Begomoviruses have emerged as overwhelming problem in various cropping systems of Pakistan. The study was conducted to evaluate the potential of induced resistance in mungbean to Mungbean yellow mosaic virus (MYMV) disease. In this work, resistance to MYMV infection was induced in mungbean plants by activating the Salicylic acid (SA) pathway using SA and Benzothiadiazole (BTH) as treatments. The resistance was characterized by evaluating symptom appearance and virus titter through ELISA. Elicitors i.e., SA and BTH were applied at different concentrations to enhance the innate resistance of mungbean by the induction of defense related compounds. All treatments were helpful in reducing plant infection but the most effective treatment was the combination of SA@5mM and BTH@150mg/L as compared to virus inoculated control. Three weeks analysis showed peak accumulation of defense related enzymatic antioxidants and phenols in the mungbean leaves treated with SA and BTH. Higher enzymatic activity was observed in elicitor treated plants followed by inoculation with MYMV. As the resistance increased due to the application of SA & BTH the enzymatic activities of SOD, POD, and CAT were also increased during second week after application of elicitors. This study revealed that SA and BTH are potential source for management of MYMV by enhancing the level of protection through induction of systemic acquired resistance.
Biological control of the post harvest diseases of fruits.Dinithi De Silva
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what is post harvest disease. Simply , Postharvest diseases are those that appear and develop after harvest. Here theses are some pictures of post harvest diseases.
Fig 01- cherry fruit rot caused by Alternaria sp.
Fig 02- mango stem end rots causative agent is Dotheiorella sp.
All postharvest diseases of fruit are caused by fungi and bacteria.
viral infections present before harvest can sometimes develop more rapidly after harvest. In general, however, viruses are not an important cause of postharvest disease. Postharvest diseases are often classified according to how infection is initiated. The so-called 'quiescent' or 'latent' infections are those where the pathogen initiates infection of the host at some point in time , but then enters a period of inactivity or dormancy until the physiological status of the host tissue changes in such a way that infection can proceed.
After The dramatic physiological changes like compositional changes physiological changes which occur during fruit ripening are often the trigger for reactivation of latent infections. It can be through direct penetration through skin, natural openings & injuries . injuries can be mechanical or caused by insects. Therefore, post harvest diseases can be arised during or after harvest.After harvest in the dramatically physiological changes like compositional changes physiological changes which occur during the fruit ripening of in triger for reactivation of the latent infection.
And also many of the physiological changes also triggers the reactivation of the latent infection mainly both the losses conditions can lead to the fungal infection because fungi are optimum at the dry conditions after that ethylene production fruit ripening cannot so it causes a lot of compositional changes in the sugar content and physiological changes in the fruit it soften the fruit covering and then it can be easily the damage so through the damage microorganisms can enter the fruits and grow inside and multiplication then causes postharvest diseases
Antioxidant activity, photosynthetic rate, and Spectral mass in bean Plants (...IJEABJ
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An increase in antioxidant activity is a common response in plants as a defense mechanism against biotic and abiotic stress factors, such response is also generated with the exogenous application of "defense activators", which have negative effects on plant metabolism. In this work, bean plants (Phaseolus vulgaris L.) cv. Pinto Nacional were treated with jasmonic acid (0.5 mM), salicylic acid (2 mM), Trichoderma asperellum (105 spores/ml), and Bacillus pumilus (105 CFU / mL), in order to determine the level of structural and metabolic response of the plants. On the seventh day after the application of the treatments, it was measured the enzymatic activity of catalase (CAT), peroxidase (POX), and superoxide dismutase (SOD). In addition, leaf impressions were taken to measure the stomatal opening and conductance, photosynthetic rate, and the mass spectrum (mass/charge, m/z). The antioxidant activity increased in plants treated with jasmonic acid and T. asperellum, which in turn significantly increased the stomatal opening and conductance, and photosynthetic rate. The mass profile showed that the plants treated with T. asperellum have a greater quantity of masses/charge, of which some had statistically highly significant difference according to the means test Tukey (p <0.05). It is concluded that some defense activators such as jasmonic acid and T. asperellum increase the antioxidant activity, defense response that concurs with the high photosynthetic and metabolic rate in bean plants.
Rhizobacteria containing ACC-deaminase confer salt tolerance to wheat (Tritic...Premier Publishers
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Wheat (Tritium aestivum) is the primary food of Pakistan.Salinity is a blockade towards growing a sustainable food production system and necessitates environment management. Plant growth promoting rhizobacteria can ameliorate abiotic stressors through induced salt tolerance. PGPR having ACC deaminase activity can improve plant performance by minimizing the effects of ACC and endogenous ethylene that limit plant growth under salt stress. Plants grown from seeds inoculated with PGPR strains having ACC deaminase are comparatively more tolerant to salt stress. The study was carried out at Soil Salinity Research Institute, PindiBhattian to investigate the impact of PGPR (Plant growth promoting Rhizobacteria) on wheat grown in salt affected field. The design was Randomized complete block with three replications. Wheat seeds Cv. Faisalbad-2008 were inoculated with rhizobacteria strains which were: Thal-8, WM-14 and WM-10 during 2015-16 Inoculation with rhizobial strains that provide ACC-deaminase activity proved a useful move toward for alleviating the stress induced by ethylene and accordingly improving the growth and yield of wheat in the presence of high salinity stress. Decline in sodium uptake following seed inoculation with different rhizobial strains for wheat grown on salt- affected lands is a constructive possibility to reclaim salt stress biologically.
This presentation was developed by Dr. A and delivered at the home garden vegetable production workshop in Selma, AL. This presentation discusses a new insecticide mode of action and correct application techniques along with several research updates.
Gene for gene system in plant fungus interactionVinod Upadhyay
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MOLECULAR CHARACTERIZATION OF GENE FOR GENE SYSTEMS IN PLANT- FUNGUS INTERACTION AND THE APPLICATIONS OF AVIRULENCE GENES IN CONTROL OF PLANT PATHOGENS
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Ioâs surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Ioâs trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Ioâs surface using adaptive
optics at visible wavelengths.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called âsmallâ because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
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What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Activation of natural host defence by elicitors for management of post harvest diseases
1. ACTIVATION OF NATURAL HOST DEFENCE BY
ELICITORS FOR MANAGEMENT OF POST
HARVEST DISEASES
Doctoral Seminar I
VINOD UPADHYAY
ID.NO- 44056
1
2. India's post-harvest losses over Rs 2 L cr annually:
Assocham
30% of Fruits and vegetables - unfit for consumption due to
spoilage after harvesting
West Bengal(Rs 13,600 crore anually) >Gujarat (Rs 11,400 crore)>
Rs (10,700 crore) >Uttar Pradesh(Rs 10,300 crore)>Maharashtra (Rs
10,100 crore)
Additional requirement of cold storage - 370 lakh tons for fruits and
vegetables. Presently- total storage capacity for 300 lakh tons.
Total postharvest food loss -10-40% (FAO, 2010)
Highlights !!
2
3. Losses would be enough to feed around 70â100 million people, i.e.
approximately a third of Indiaâs poor (ICAR, 2010)
Loss of overall ResourcesâŠ.seed, labor, water..etc.
Food Security and Postharvest Loss
3
4. INTRODUCTION
ïCompounds stimulating any type of plant defense
ï Biotic or abiotic origin
ï Production of ROS (reactive oxygen species)
ï Hypersensitive response
ï Production of phytoalexins, i.e. antimicrobial secondary
compounds
ELICITOR
Induction of plant defense
Elicitors may induce local acquired resistance (LAR), systemic acquired
resistance (SAR) or induced systemic resistance (ISR)
4
10. NDR declines: Results in activation of quiescent infections
Factors affecting decline of NDR in produce after harvest :
(1) Nutritional requirements for the pathogen
(2) Preformed antifungal compounds (phytoanticipins)
(3) Potential for inducible antifungal compounds (phytoalexins)
(4) Activation of fungal pathogenicity factors
Prusky, 1996
NATURAL DISEASE RESISTANCE (NDR) AFTER
HARVEST
After harvest
1
0
11. ï Modify the plant-pathogen interaction by resembling an
incompatible interaction with defense-related mechanisms
ï Mimic the action of the signaling molecules SA and JA and their
derivates
ï Luckey (1980) - concept of inducing NDR âplant hormesisâ
ï Stimulation of a beneïŹcial plant response by low or sub-lethal
doses of an elicitor/agent
PLANT HORMESIS
11
12. ï No risk of creating resistant pathogen strains
ï Increase the phenolic content of fruit
ï Protecting plants and fruits from biotic and abiotic stresses
ï No impact on environment
ï Pre harvest spray can also control postharvest losses
WHY TO FOCUS ON ELICITORS ?????
1
2
19. HARPIN TREATED BEFORE HARVEST HARPIN TREATED AFTER HARVEST
120 days at 0.5°C plus 7 days at
20°C
120 days at 0.5°C
Diseasefruit
%
104spores/ml
103spores/ml
Capdeville et al.,
Diseasefruit
%
19
26. EFFECTS OF BENZOTHIADIAZOLE AND METHYL JASMONATE ON
BANANAS INOCULATED WITH Colletotrichum musae
Cheng Ma et
BTH- 5 mmol/L
MeJA - 0.1 mmol /L
At 22ÂșC 2
6
27. EFFECTS OF BENZOTHIADIAZOLE AND
METHYL JASMONATE ON CHITINASE (MaChit) GENE
EXPRESSION
Cheng Ma et
al.,2009
2
7
33. Effects of pre-harvest ASM spray in Yali pear fruit
inoculated with P. expansum or A. alternata after harvest
P. expansum
A.
alternata
Cao et al.,2006
3
3
34. Effects of pre-harvest ASM spray on the activities of
defence related enzymes in Yali pear fruit after harvest
Cao et al.,2006
3
4
35. Cao et al.,2006
Effect of pre-harvest spray of ASM on activities of
different enzymes in young harvested pear fruit
3
5
42. Compatibility of methyl jasmonates with antagonistic
yeast Cryptococcus laurentii
Yao et25ÂșC 0ÂșC
Me Ja conc.
0”mol/l
50”mol/l
100”mol/l
200”mol/l
400”mol/l
4
2
43. Effect of Monilinia fructicola and Penicillium expansum
in peach fruit treated with MeJA and Cryptococcus
laurentii
Yao et
al.,2004
CK-Wounding +
pathogen
A- MeJA + pathogen
B- C. laurentii +
pathogen(less cfu)
C- MeJA
+C.laurentii
+ pathogen
D- C. laurentii +
pathogen(high cfu)
M. fructicola P. expansum
4
3
44. Effect of MeJA and Cryptococcus laurentii on
defense related enzymes in peach fruit
Yao et
25ÂșC 0ÂșC
4
4
45. Effect of MeJA and Cryptococcus laurentii on PAL
AND POD activity in peach fruit
Yao et
25ÂșC 0ÂșC
4
5
48. Comparative effects of different elicitors on
management of Alternaria rot in pear
Tian et al., 2006
A - Salicylic acid
B - oxalic acid
C- calcium chloride
D - antagonistic yeast
E- untreated control
4
8
49. Comparative effects of different elicitors on
defense related enzymes in pear
Tian et al., 2006
4
9
50. FACTORS INFLUENCING THE EXPRESSION
OF INDUCED RESISTANCE
GENOTYPE
ENVIRONMENT
OTHER FACTORS
5
0
Walters et al.,2005
51. GENOTYPE:
Hijwegen and Verhaar (1994) - Resistance in cucumber to the powdery
mildew fungus induced by treatment with INA was cultivar dependent
ïHighest levels of induced resistance expressed in a partially resistant
cultivar
ïmuch lower levels of resistance in susceptible cultivars
Dann et al.(1998) - Resistance to Sclerotinia sclerotiorum induced by
treatment with INA or ASM in soybean was greatest in susceptible
cultivars
Oostendorp et al. (2001)- Resistance activated by ASM in monocots
are very long lasting compare to dicots
5
1
52. Heil et al.(2000) - ASM applied to wheat in the absence of pathogen
ï Reduced biomass
ï Reduced numbers of ears and grains
ï When nitrogen supply was limited effects was pronounced
Reductions in the expression of genes related to primary metabolism
following elicitation of resistance.
Dietrich et al. (2005)- Treated Arabidopsis plants with ASM -initial growth
reductions was compensated subsequently by increasing growth rates
ENVIRONMENT
COST OF INDUCED RESISTANCE
5
2
53. ï Effect of resistance induction on seed production was determined by
environmental conditions such as nitrogen supply, water stress, and
competition with other plants.
ï Result - costs, no costs, or even higher seed production by ASM-induced
compared with uninduced controls under different combinations of
environmental factors.
Induced plants were most likely to incur fitness costs if grown in a
competitive environment.
Dietrich et al.(2005)
5
3
54. Should a resistance elicitor be applied before or after a fungicide
application????
Depend upon the particular pathogen or pathogens being targeted and the stage
of crop growth
OTHER FACTORS
ï Eg. Strobilurin fungicide + ASM - effective in controlling Albugo occidentalis
and increasing leaf quality in spinach
ïASM + mancozeb- provide protection against Claviceps africana on
sorghum in case where fungal isolates resistant to the usual fungicide treatment
-triadimenol
ï Timing of application and frequency of application
ï Conjunction with appropriate-dose fungicides
COMBINATIONS
54
55. QUESTION NEED TO BE ANSWERED
Will incorporation of induced resistance into disease control programs
be more expensive than current approaches?
Will induced resistance represent a sustainable approach to disease
control???
Farmers and growers need to be convincedâŠ.
Whether it can replace the existing management practices????
Will it be feasible with other practice in integrated disease
management?????
55