Plant pathology is the study of plant diseases. Major plant pathogens include fungi, bacteria, nematodes, and viruses. Techniques used to detect pathogens include histopathological examination of infected tissues, culture growth on media, staining, microscopy, and analysis of biochemical properties. Symptoms caused by pathogens can provide clues to identification. Isolation and purification of the pathogen allows for reinoculation to fulfill Koch's postulates.