1. Plant-fungus interactions can be characterized by gene-for-gene systems where a plant resistance gene corresponds to a fungal avirulence gene. Vertical or race-specific resistance follows this pattern and is not durable due to high selection pressure.
2. R proteins in plants recognize specific pathogen effectors or avirulence proteins through direct or indirect models. Direct models involve recognition of effectors by R protein receptors. Indirect models involve the effector targeting or modifying a host protein that is then recognized by the R protein.
3. Understanding gene-for-gene systems and how plants recognize pathogens at the molecular level can enable new strategies for disease control through deployment of resistance genes and exploitation of avirulence
Molecular basis of plant resistance and defense responses to pathogensSenthil Natesan
In response to pathogen attack, plants have evolved sophisticated defense mechanisms to delay or arrest pathogen growth.Unlike animals, plants lack a circulating immune system recognizing microbial pathogens. Plant cells are more autonomous in their defense mechanisms and rely on the innate immune capacity of each cell and systemic signals that disseminate from infection sites (Jones and Dangl, 2006). Plant innate immunity consists of preformed physical and chemical barriers (such as leaf hairs, rigid cell walls, pre-existing antimicrobial compounds) and induced defenses. Should an invading microbe successfully breach the pre-formed barriers, it may be recognized by the plant, resulting in the activation of cellular defense responses that stop or restrict further development of the invader.
Molecular basis of plant resistance and defense responses to pathogensSenthil Natesan
In response to pathogen attack, plants have evolved sophisticated defense mechanisms to delay or arrest pathogen growth.Unlike animals, plants lack a circulating immune system recognizing microbial pathogens. Plant cells are more autonomous in their defense mechanisms and rely on the innate immune capacity of each cell and systemic signals that disseminate from infection sites (Jones and Dangl, 2006). Plant innate immunity consists of preformed physical and chemical barriers (such as leaf hairs, rigid cell walls, pre-existing antimicrobial compounds) and induced defenses. Should an invading microbe successfully breach the pre-formed barriers, it may be recognized by the plant, resulting in the activation of cellular defense responses that stop or restrict further development of the invader.
plant pathogen interaction
different types of pathogens
gene for gene hypothesis
direct receptor model
Elicitor receptor model
suppersor repressor model
gaurd hypothesis
The concept of gene for gene hypothesis was first developed by Flor in 1956 based on his studies of host pathogen interaction in flax, for rust caused by Melampsora lini. The gene for gene hypothesis states that for each gene controlling resistance in the host, there is corresponding gene controlling pathogenicity in the pathogen. The resistance of host is governed by dominant genes and virulence of pathogen by recessive genes. The genotype of host and pathogen determine the disease reaction. When genes in host and pathogen match for all loci, then only the host will show susceptible reaction. If some gene loci remain unmatched, the host will show resistant reaction. Now gene – for –gene relationship has been reported in several other crops like potato, sorghum, wheat, etc. The gene for gene hypothesis is also known as “Flor Hypothesis.”
Plants have array of defense response against biotic stresses which could be either structural reinforcement, release of chemicals, and defense gene expression against invading organisms. The physical barriers are trichoms, waxy cuticle, thick cell wall. Once the pathogen overcomes the first line of defense, basal or innate defense response comes into play. Pathogens secrete some conserved molecules known as Pathogen Associated Molecular Pattern (PAMP/MAMP), which are recognized by transmembrane receptors present in the plasma membrane and initiate a series of signal cascade reaction which ultimately leads to activation of various defense related genes. Apart from inducing the expression of defense related genes, it also triggers a hypersensitive reaction (HR) which cause deliberate cell death at the site of infection and limit the pathogen access to water and nutrient by sacrificing a few cells in order to save the rest of the plant. Once HR is triggered, plant tissue may become highly resistant to a broad range of pathogens for an extended period of time. This phenomenon is called Systemic Acquired Resistance (SAR).
Plants respond to herbivory is a similar manner as described above. The biochemical mechanisms of defense against the herbivores are wide-ranging, highly dynamic, and are mediated both by direct and indirect defenses. The defensive compounds are either produced constitutively or in response to plant damage, and affect feeding, growth, and survival of herbivores. In addition, plants also release volatile organic compounds that attract the natural enemies of the herbivores. These strategies either act independently or in conjunction with each other. However, our understanding of these defensive mechanisms is still limited. Induced resistance could be exploited as an important tool for the pest management to minimize the amounts of insecticides used for pest control. Host plant resistance to insects, particularly, induced resistance, can also be manipulated with the use of chemical elicitors of secondary metabolites, which confer resistance to insects. By understanding the mechanisms of induced resistance, we can predict the herbivores that are likely to be affected by inducing responses. The elicitors of induced responses can be sprayed on crop plants to build up the natural defense system against damage caused by herbivores. The induced responses can also be genetically engineered, so that the defensive compounds are constitutively produced in plants challenged by the herbivory. Induced resistance can be exploited for developing crop cultivars, which readily produce the inducible response upon mild infestation, and can act as one of components of integrated pest management for sustainable crop production.
M.Sc. (Master's) Seminar on topic "Role of chemicals in plant disease managem...Harshvardhan Gaikwad
The importance and role of chemicals/ fungicides in plant disease management is the major objective of plant pathology. The need based, effective, ecofriendly application of chemical fungicides can leads sustainable agriculture and food production.
Plant - Pathogen Interaction and Disease DevelopmentKK CHANDEL
Plant diseases are the result of infection by any living organisms that adversely affect the growth, development, physiological functioning and productivity of a plant, manifesting outwardly as visible symptoms.
plant pathogen interaction
different types of pathogens
gene for gene hypothesis
direct receptor model
Elicitor receptor model
suppersor repressor model
gaurd hypothesis
The concept of gene for gene hypothesis was first developed by Flor in 1956 based on his studies of host pathogen interaction in flax, for rust caused by Melampsora lini. The gene for gene hypothesis states that for each gene controlling resistance in the host, there is corresponding gene controlling pathogenicity in the pathogen. The resistance of host is governed by dominant genes and virulence of pathogen by recessive genes. The genotype of host and pathogen determine the disease reaction. When genes in host and pathogen match for all loci, then only the host will show susceptible reaction. If some gene loci remain unmatched, the host will show resistant reaction. Now gene – for –gene relationship has been reported in several other crops like potato, sorghum, wheat, etc. The gene for gene hypothesis is also known as “Flor Hypothesis.”
Plants have array of defense response against biotic stresses which could be either structural reinforcement, release of chemicals, and defense gene expression against invading organisms. The physical barriers are trichoms, waxy cuticle, thick cell wall. Once the pathogen overcomes the first line of defense, basal or innate defense response comes into play. Pathogens secrete some conserved molecules known as Pathogen Associated Molecular Pattern (PAMP/MAMP), which are recognized by transmembrane receptors present in the plasma membrane and initiate a series of signal cascade reaction which ultimately leads to activation of various defense related genes. Apart from inducing the expression of defense related genes, it also triggers a hypersensitive reaction (HR) which cause deliberate cell death at the site of infection and limit the pathogen access to water and nutrient by sacrificing a few cells in order to save the rest of the plant. Once HR is triggered, plant tissue may become highly resistant to a broad range of pathogens for an extended period of time. This phenomenon is called Systemic Acquired Resistance (SAR).
Plants respond to herbivory is a similar manner as described above. The biochemical mechanisms of defense against the herbivores are wide-ranging, highly dynamic, and are mediated both by direct and indirect defenses. The defensive compounds are either produced constitutively or in response to plant damage, and affect feeding, growth, and survival of herbivores. In addition, plants also release volatile organic compounds that attract the natural enemies of the herbivores. These strategies either act independently or in conjunction with each other. However, our understanding of these defensive mechanisms is still limited. Induced resistance could be exploited as an important tool for the pest management to minimize the amounts of insecticides used for pest control. Host plant resistance to insects, particularly, induced resistance, can also be manipulated with the use of chemical elicitors of secondary metabolites, which confer resistance to insects. By understanding the mechanisms of induced resistance, we can predict the herbivores that are likely to be affected by inducing responses. The elicitors of induced responses can be sprayed on crop plants to build up the natural defense system against damage caused by herbivores. The induced responses can also be genetically engineered, so that the defensive compounds are constitutively produced in plants challenged by the herbivory. Induced resistance can be exploited for developing crop cultivars, which readily produce the inducible response upon mild infestation, and can act as one of components of integrated pest management for sustainable crop production.
M.Sc. (Master's) Seminar on topic "Role of chemicals in plant disease managem...Harshvardhan Gaikwad
The importance and role of chemicals/ fungicides in plant disease management is the major objective of plant pathology. The need based, effective, ecofriendly application of chemical fungicides can leads sustainable agriculture and food production.
Plant - Pathogen Interaction and Disease DevelopmentKK CHANDEL
Plant diseases are the result of infection by any living organisms that adversely affect the growth, development, physiological functioning and productivity of a plant, manifesting outwardly as visible symptoms.
Management of host plant resistance through immunizationAnshul Arya
it is a small presentation prepared for seminar purpose .immunization is a new technique very few people know about it even i did not get any slide prepared by it earlier even whatever i got was not purchased .so i prepared it for those who are interested to know about it without having problems to find the matter for it.
Content:
Introduction
Importance of Host Plant Resistance
Historical perspectives
Advantages and Disadvantages of HPR
Mechanisms of Resistance
Adaptation of Resistance in Plant to Insect
Morphological
Anatomical
Biochemical
Assembly of plant species - Gene Pool
Behavior in Relation to Host Plant Factor
Functional Genomics of Plant Pathogen interactions in Wheat Rust PathosystemSenthil Natesan
Cereal rust fungi are pathogens of major importance to agriculture, threatening cereal production worldwide. Targeted breeding for resistance, based on information from fungal surveys and population structure analyses of virulence, has been effective. Nevertheless, breakdown of resistance occurs frequently and continued efforts are needed to understand how these fungi overcome resistance and to determine the range of available resistance genes. The development of genomic resources for these fungi and their comparison has released a torrent of new ideas and approaches to use this information to assist pathologists and agriculture in general. The sequencing of gene transcripts and the analysis of proteins from haustoria has yielded candidate virulence factors among which could be defence-triggering avirulence genes. Genome-wide computational analyses, including genetic mapping and transcript analyses by RNA sequencing of many fungal isolates, will predict many more candidates (Bakkeren et al., 2012)
Dissecting the mechanisms of host-pathogen systems like wheat-rust, including pathogen counter-defenses will ensure a step ahead towards understanding current outcomes of interactions from a co-evolutionary point of view, and eventually move a step forward in building more durable strategies for management of diseases caused by fungi (Hadrami et al.,2012)
Detection of ug99_in_egypt_bgri2015_austrilia_atef_shahinAtef Shahin
Wheat stem rust caused by Puccinia graminis f. sp. tritici = Pgt play an important role in wheat production throughout the world with rusts disease of wheat (Triticum aestivum L.).
Keynote presentation from Plant and Pathogen Bioinformatics workshop at EMBL-EBI, 8-11 July 2014
Slides and teaching material are available at https://github.com/widdowquinn/Teaching-EMBL-Plant-Path-Genomics
B4FA 2012 Nigeria: Maruca-resistant Cowpea Research in Nigeria - Muhammad Lawanb4fa
Presentation by Muhammad Lawan, Ahmadu Bello University, Zaria, Nigeria
Delivered at the B4FA Media Dialogue Workshop, Ibadan, Nigeria - September 2012
www.b4fa.org
Host Perception and Signaling During Bacterial InfectionsSuresh Gopalan
This is one of my past work on host-bacterial interactions. results have broad applicability and not specific to plant models used. Please contact me if you need a clean copy.
See text at http://molcyt.org/2012/11/29/superdomestication-feed-forward-breeding-and-climate-proofing-crops/ which also links the the YouTube talk using these slides
History
Host pathogen interaction
R gene
Molecular techniques for detection of plant pathogens
Role of molecular techniques in resistance breeding Deployment of R genes and linked markers
Transgenic approaches in plant protection
Conclusion
Plant disease resistance genes: current status and future directions.RonikaThakur
Agriculture plays a key role to ensure the food security. But plant diseases hinder the crop production by reducing yield to much extent. To overcome this problem it is crucial to understand plant disease resistance genes which prevent growth of plant pathogens thereby reducing the yield loss.
Systemic Acquired Resistance (SAR) and it’s Significance in Plant Disease Ma...Ankit Chaudhari
Systemic Acquired Resistance (SAR) is a mechanism of induced defense that confers long-lasting protection against a broad spectrum of microorganisms and pests. Presently disease control is largely based on the use of hazardous chemicals viz., fungicides, bactericides and insecticides for either direct or indirect disease management. The hazardous natures of the products on the environment, human and animal health strongly necessitates the search for new safer means of disease control. SAR have high potential to diminish the use of toxic chemicals in the agriculture and has emerged as an alternative, non-conventional, non-biocidal and eco-friendly approach for plant protection and hence for sustainable agriculture. SAR requires the signal molecule salicylic acid (SA) and is associated with accumulation of pathogenesis-related proteins, which are thought to contribute to resistance.
Gene silencing techniques for crop improvementJhilickBanerjee
Gene silencing is a technique that aims to reduce or eliminate the production of a protein from its corresponding gene. Gene silencing is the regulation of gene expression in a cell.
Gene silencing can occur during either transcription or translation.
Gene silencing is often considered as “Gene knockdown’ i.e their expression is reduced. In contrast , when genes are knocked out they are completely erased from the organism’s genome and thus have no expression.
Methods used to silence genes include RNAi, CRISPR or siRNA, these reduce the expression of the gene by 70% but do not completely eliminate it.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Nucleic Acid-its structural and functional complexity.
Gene for gene system in plant fungus interaction
1. MOLECULAR CHARACTERIZATION OF GENE FOR GENE
SYSTEMS IN PLANT- FUNGUS INTERACTION AND THE
APPLICATIONS OF AVIRULENCE GENES IN CONTROL OF
PLANT PATHOGENS
1
2. NON HOST RESISTANCE :
• Apple trees and tomato pathogens
• Powdery mildew on wheat (Blumeria graminis f. sp. tritici)
and barley
Disease is exception rather than rule
HORIZONTAL RESISTANCE :
• General resistance, quantitative resistance
• Incomplete resistance but durable
2
3. VERTICAL RESISTANCE :
• Race specific , qualitative resistance, differential resistance
• Complete resistance, not durable – high selection presssure
• Follow gene for gene system
3
PATHOGEN
RACE
PATHOGEN RACE
PLANT VARIETY 1 2 PLANT VARIETY 1 2 3 4
A _ + A _ + + +
B + _ B + _ _ +
C _ + _ +
D + _ + _
4. Reaction of plants to attacks by various pathogens in relation to resistance of
the plant
4Agrios (2005)
5. Each gene that confers avirulence (Avr) to the pathogen there is a
corresponding gene in the host that confers resistance (R) to the host
and vice versa – H.H.Flor (1946)
5
RESISTANT OR SUSCEPTIBILITY GENES IN THE
PLANT
VIRULENCE OR AVIRULENCE
GENE IN PATHOGEN
R (resistant)
dominant
r ( susceptibility)
recessive
A (avirulent) dominant AR (-) Ar (+)
a ( virulent) recessive Ar (+) ar (+)
6. 6
RESISTANCE (R) OR SUSCEPTIBILITY ( r) GENES
IN THE PLANT
R1 R2 R1 r2 r1 R2 r1 r2
VIRULENCE (a) OR
AVIRULENCE (A)
GENES IN THE
PATHOGEN
A1 A2 - - - +
A1 a2 - - + +
a1 A2 - + - +
a1 a2 + + + +
10. Hydrophilic- lacking stretches of hydrophobic amino acids - enable
them to be anchored in cell membranes
May produced and localized in pathogen cytoplasm or secreted
through membrane pores
If secreted externally – directly acts as elicitors
If localized in cytoplasm of pathogen - acts indirectly as enzyme to
produce elicitor molecules
Acting as avirulence factors in elicitor-receptor model (plant
defense)
10
11. Contribution towards the virulence of pathogen. eg. AvrBs2 gene of
X. campestris pv. Vesicatoria
Avr proteins interact with specific plant proteins (virulence target) -
enhances availability of nutrients to pathogen .
Most R proteins contain amino acid leucine rich domain (LRR-
leucine rich repeats),
Depending on R protein LRR reside : cytoplasmic LRRs or
extracytoplasmic LRRs.
Leucine-rich repeats (LRR) region of R-genes is involved in
recognizing pathogens
11
12. MAJOR CLASSES OF R PROTEINS
S. NO MAJOR R-GENE CLASSES EXAMPLE
1 NBS-LRR-TIR N, L6, RPP5
2 NBS-LRR-CC I2, RPS2, RPM1
3 LRR-TrD Cf-9, Cf-4, Cf-2
4 LRR-TrD-Kinase Xa21
5 TrD-CC RPW8
6 TIR-NBS-LRR-NLS- WRKY RRS1R
7 LRR-TrD-PEST-ECS Ve1, Ve2
8 Enzymatic R-genes Pto, Rpg1
LRR - Leucine rich repeats; NBS - Nucleotide-binding site; TIR -Toll/Interleukin-
1- receptors; CC - Coiled coil; TrD –Transmembrane domain; PEST -Amino acid
domain; ECS - Endocytosis cell signaling domain; NLS - Nuclear localization
signal; WRKY -Amino acid domain; HC toxin reductase - Helminthosporium
carbonum toxin reductase enzyme.
12Gururani et.al.,2012
15. Plant proteins belonging to
the nucleotide-binding site–
leucine-rich repeat (NBS-
LRR) family are used for
pathogen detection.
(R-PROTEIN)
Harmful organism
Recognition by
resistance protein
Signal to cell
nucleus
Genetic
material
Defense
Response Defense protein
(R-PROTEIN)
Outsideplantcell
Insideplantcell
Diagram of a plant disease resistance protein in action. A portion of the protein
(MAROON) lies outside the cell and specifically recognises the harmful organism.
The remaining portion of the protein (RED) resides inside the cell and
communicates a signal to the plant’s genetic material, which in turn stimulates a
defense response against the invading organism.
(R-GENE)
15
16. • INDIRECT PERCEPTION OF AVR PROTEINS:
Protease dependent defense elicitation model
The co-receptor model
The guard hypothesis
The decoy hypothesis
Bait and Switch model
•DIRECT PERCEPTION OF AVR PROTEINS :
Elicitor- receptor model
16
17. Albersheim and Anderson Prouty, 1975 proposed this model.
Avirulence (Avr) gene of a pathogen encodes an elicitor (Avr) protein
that is recognized by a receptor protein encoded by the matching
resistance (R) gene of the host plant.
eg. Pi-ta R gene from rice and AvrPi-ta from Magnaporthe grisea
ELICITOR– RECEPTOR MODEL
17Staskawicz et.al.,1995
18. PROTEASE DEPENDENT DEFENSE ELICITATION
MODEL
Kruger et al., 2002 proposed this model.
eg.Tomato leaf mold –Cladosporium fulvum interaction.
Rcr3 required for Cf-2 mediated resistance towards C.fulvum
strains carrying Avr2, encodes a tomato cysteine endoprotease.
Rcr3 might process Avr2 to generate a mature ligand, or Rcr3
might degrade Avr2 - releasing active elicitor peptides that
interact with the extracellular LRR of Cf2.
18
20. THE CO-RECEPTOR MODEL
Jones and Jones,1996 proposed this model.
RPS5 an Arabidopsis NB-LRR protein localized to a membrane
fraction - activated by the AvrPphB cysteine protease effector from P.
syringae.
AvrPphB is cleaved, acylated and delivered to the host plasma
membrane. Activated AvrPphB cleaves the Arabidopsis PBS1 serine-
threonine protein kinase, leading to RPS5 activation.
20
22. THE GUARD HYPOTHESIS
Van-der-biezen and Jones, 1998 proposed this model.
Interaction between guardee and Avr is recognized by the R
protein
eg. AvrPto of Pseudomonas syringae and Pto gene of tomato,
Prf gene act as guardee.
Evolutionary unstable situation
R protein is absent -evolution of the guardee to avoid binding
R protein is present - selection will favor binding
22
24. Van der Hoorn and Kamoun, 2008 proposed this model.
Host protein termed as “decoy” - specializes in perception of the
effector by the R protein
Not contributing pathogen fitness in the absence of its cognate R
protein.
Effector target monitored by the R protein is a decoy that mimics
the operative effector target
e.g. AvrPto and AvrBs3 - some host targets of effectors act as
decoys to detect pathogen effectors via R proteins
DECOY HYPOTHESIS
24
26. Peter Moffett , proposed this model in 2002.
The NB-LRR protein - primed (signaling competent) but
autoinhibited (restrained from signaling) state.
Functional nucleotide binding pocket and multiple intramolecular
interactions - fine-tuned balance between the LRR and ARC2.
Avr protein is brought into the NB-LRR system via the bait protein
- direct binding or alteration to bait.
Conformational changes within the nucleotide binding pocket-
allow signaling motif - downstream signaling components.
Subsequent to signaling, intramolecular interactions within the NB-
LRR protein dissociated.
BAIT AND SWITCH MODEL
26
44. FLAX RUST –
All the virulent rust strains retain intact copies of the Avr genes
(AvrL567) but have altered their sequences
Host R genes imposed selection for new variants to escape recognition.
44
Jones & Dangl (2006)
47. Functional genomic tools to disease resistance - interactions between
defense signaling and other plant processes.
Structural basis of recognition will enable us - design R proteins that
recognize essential virulence factors
New transgenic resistant plants by exploiting both avirulence genes
and resistance genes in molecular resistance breeding
Using avirulence gene products / race-specific elicitors - events in
signal transduction pathways can be studied.
47
Fig. 3. Comparison of the guard model and the decoy model.
A: the guard model. Multiple effectors could be perceived by a single R protein, and a relatively small number of R genes could target the broad spectrum of
pathogens that attack plants. B: the decoy model. The guardee is in an evolutionarily unstable situation named as ‘decoy’. In the presence of functional R genes,
natural selection is expected to favor guardees with improved interaction with an effector to enhance pathogen detection. In the absence of R genes, natural
selection is expected to drive the guardee to decrease its binding affinity with the effector and evade detection and modification by the effector.
FIGURE 1 Gene-for-gene resistance. Dominant resistance (R) genes confer resistance
to specific pathogens. This resistance is dependent on whether the pathogen
possesses a matching avirulence (Avr) gene or not (avr). Asterisks and lighter gray
shading indicate that in many cases, mutation or deletion of the Avr gene results in
a fitness cost for the pathogen such that although it gains virulence on resistant
hosts, it suffers a fitness penalty on susceptible hosts.