Fungal culture methods and media are described. Most molds grow best at 25-30°C while most yeasts grow best at 35-37°C. General incubation is 14 days but it can be as short as 7 days for detecting oral yeast or as long as 28 days for tissue specimens. ChromAgar differentially identifies Candida species by color. Chlamydospore agar induces chlamydospore formation in C. albicans. Sabhi agar is recommended for recovering fungi from clinical specimens. Czapek's agar is a synthetic medium used to observe fungal morphology and pigment production.
A discussion on the media and biochemical tests as discussed by Ms. Caryl Villalon, RN, MT. Covers the descriptions of the media and biochemical tests. How to perform the tests, properties of the tests, media and reagents used, and the results of the test. Pictures of positive and negative results are also shown in the slide.
COLLECTION AND TRANSPORTATION OF CLINICAL SAMPLESNCRIMS, Meerut
Principles of Sample Collection:
Aseptic precautions to minimize chances of
contamination.
Appropriate anatomic sites
Adequate volume
Adequate no. of samples
Appropriate time
Appropriate container with proper labelling
Before initiation of anti-microbials
Adequate information in request form
A discussion on the media and biochemical tests as discussed by Ms. Caryl Villalon, RN, MT. Covers the descriptions of the media and biochemical tests. How to perform the tests, properties of the tests, media and reagents used, and the results of the test. Pictures of positive and negative results are also shown in the slide.
COLLECTION AND TRANSPORTATION OF CLINICAL SAMPLESNCRIMS, Meerut
Principles of Sample Collection:
Aseptic precautions to minimize chances of
contamination.
Appropriate anatomic sites
Adequate volume
Adequate no. of samples
Appropriate time
Appropriate container with proper labelling
Before initiation of anti-microbials
Adequate information in request form
go to www.medicaldump.com to download this medical powerpoint presentation. You can find medical templates, medical pdfs and medical powerpoints on all specialties
Screening, identification and isolation of cellulolytic fungiDr. sreeremya S
Cellulase assay for Enzyme production
The activity of -glucosidase ( G), total
cellulase (FPase) and endoglucanase
(CMCase) was studied as cellulolytic
activity. Filter paper activity (FPase) for
total cellulase activity in the culture filtrate
was determined according to the standard
method (Eveleigh DE et al.2009). CMCase
(carboxy methyl cellulase) activity was
assayed using Dinitrosalicylic acid (DNS)
method (Mandels and Weber, 1969).
Speckled Monster Activity A - Teacher PresentationEmma Hill
Children take part in a simulation of a smallpox outbreak which introduces them to the observations that led Edward Jenner to his world-changing discovery that cowpox gives immunity to the dreaded ‘Speckled Monster’.
The enterobacteriaceae basic properties.ppsx xNursing Path
The Enterobacteriaceae are a large family of Gram-negative bacteria that includes, along with many harmless symbionts, many of the more familiar pathogens, such as Salmonella, Escherichia coli, Yersinia pestis, Klebsiella, and Shigella.
Isolation and Molecular Characterization of Pullulanase Producing Bacillus St...YogeshIJTSRD
Pullulanase is an extracellular carbohydrase responsible for the hydrolysis of pullulan and amylopectin toproduce maltotriose. The product maltotriose is used in detergent industry, bakery industry and in the production of biotechnological products. In the present investigation pullulanase producing bacillus species were isolated and characterized using different biochemical and molecular methodologies. The isolates were identified as Bacillus cereus and Bacillus thuringiensis respectively.. The pullulanase acivity was higher in Bacillus cereus, 0.62U ml than B. thuringiensis, 0.53 U ml. This research reveals that pullulanase enzyme production from these Bacillus species shows great promise for use in industrial processes. Nwozor, N. C | Ogbo, F. C "Isolation and Molecular Characterization of Pullulanase Producing Bacillus Strains" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd45051.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/45051/isolation-and-molecular-characterization-of-pullulanase-producing-bacillus-strains/nwozor-n-c
Biochemical tests for bacterial identificationSuprakash Das
Basic biochemical tests for identification of most common bacteria along with their principles and methods to perform and quality control for UG & PG Students.
Innovation is at the core of the life sciences sector and Belgium has a track record of developing ingenious ways to improve the quality of life of millions of people worldwide.
Not only extensive life sciences facilities support this high-level technology industry but also financial benefits for R&D companies, international collaborations that guarantee regular cash flow, world known educational systems, a promising product pipeline, and Belgium’s unique central location in Europe.
Belgium concentrates on a small territory (30,528 km2) more than 300 life sciences companies with biotech activities. In total, the life sciences sector employs more than 30,000 people (low bound estimation that does not take into account indirect employment generated by the sector). The majority of the companies is related to healthcare, but Belgium also has a strong representation of agricultural and industrial biotech companies.
The growing life sciences sector gathers 8 main Belgian universities, 19 research parks and 23 incubators, research institutes, academic hospitals, and clinical research organizations. Biotech companies have access to more than 500,000 square feet of highly flexible infrastructure.
Additional assets of Belgium include: a supportive regulatory and political environment, a highly qualified and productive workforce, a competitive tax environment especially for research companies, a prominent location and an
excellent logistical and business infrastructure.
Taylor created XL (Xylose Lysine) Agar Base to isolate and differentiate Gram-negative enteric bacteria.
Sodium thiosulfate, ferric ammonium citrate, and sodium deoxycholate were added to XL Agar Base to create XLD Agar, a more selective medium.
Using numerous staining chemicals, George Chapman and his colleagues at The Clinical Research Laboratory in New York produced a series of isolation media in the 1930s and 1940s.
UiPath Test Automation using UiPath Test Suite series, part 4DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 4. In this session, we will cover Test Manager overview along with SAP heatmap.
The UiPath Test Manager overview with SAP heatmap webinar offers a concise yet comprehensive exploration of the role of a Test Manager within SAP environments, coupled with the utilization of heatmaps for effective testing strategies.
Participants will gain insights into the responsibilities, challenges, and best practices associated with test management in SAP projects. Additionally, the webinar delves into the significance of heatmaps as a visual aid for identifying testing priorities, areas of risk, and resource allocation within SAP landscapes. Through this session, attendees can expect to enhance their understanding of test management principles while learning practical approaches to optimize testing processes in SAP environments using heatmap visualization techniques
What will you get from this session?
1. Insights into SAP testing best practices
2. Heatmap utilization for testing
3. Optimization of testing processes
4. Demo
Topics covered:
Execution from the test manager
Orchestrator execution result
Defect reporting
SAP heatmap example with demo
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Kubernetes & AI - Beauty and the Beast !?! @KCD Istanbul 2024Tobias Schneck
As AI technology is pushing into IT I was wondering myself, as an “infrastructure container kubernetes guy”, how get this fancy AI technology get managed from an infrastructure operational view? Is it possible to apply our lovely cloud native principals as well? What benefit’s both technologies could bring to each other?
Let me take this questions and provide you a short journey through existing deployment models and use cases for AI software. On practical examples, we discuss what cloud/on-premise strategy we may need for applying it to our own infrastructure to get it to work from an enterprise perspective. I want to give an overview about infrastructure requirements and technologies, what could be beneficial or limiting your AI use cases in an enterprise environment. An interactive Demo will give you some insides, what approaches I got already working for real.
DevOps and Testing slides at DASA ConnectKari Kakkonen
My and Rik Marselis slides at 30.5.2024 DASA Connect conference. We discuss about what is testing, then what is agile testing and finally what is Testing in DevOps. Finally we had lovely workshop with the participants trying to find out different ways to think about quality and testing in different parts of the DevOps infinity loop.
Neuro-symbolic is not enough, we need neuro-*semantic*Frank van Harmelen
Neuro-symbolic (NeSy) AI is on the rise. However, simply machine learning on just any symbolic structure is not sufficient to really harvest the gains of NeSy. These will only be gained when the symbolic structures have an actual semantics. I give an operational definition of semantics as “predictable inference”.
All of this illustrated with link prediction over knowledge graphs, but the argument is general.
The Art of the Pitch: WordPress Relationships and SalesLaura Byrne
Clients don’t know what they don’t know. What web solutions are right for them? How does WordPress come into the picture? How do you make sure you understand scope and timeline? What do you do if sometime changes?
All these questions and more will be explored as we talk about matching clients’ needs with what your agency offers without pulling teeth or pulling your hair out. Practical tips, and strategies for successful relationship building that leads to closing the deal.
Securing your Kubernetes cluster_ a step-by-step guide to success !KatiaHIMEUR1
Today, after several years of existence, an extremely active community and an ultra-dynamic ecosystem, Kubernetes has established itself as the de facto standard in container orchestration. Thanks to a wide range of managed services, it has never been so easy to set up a ready-to-use Kubernetes cluster.
However, this ease of use means that the subject of security in Kubernetes is often left for later, or even neglected. This exposes companies to significant risks.
In this talk, I'll show you step-by-step how to secure your Kubernetes cluster for greater peace of mind and reliability.
Transcript: Selling digital books in 2024: Insights from industry leaders - T...BookNet Canada
The publishing industry has been selling digital audiobooks and ebooks for over a decade and has found its groove. What’s changed? What has stayed the same? Where do we go from here? Join a group of leading sales peers from across the industry for a conversation about the lessons learned since the popularization of digital books, best practices, digital book supply chain management, and more.
Link to video recording: https://bnctechforum.ca/sessions/selling-digital-books-in-2024-insights-from-industry-leaders/
Presented by BookNet Canada on May 28, 2024, with support from the Department of Canadian Heritage.
UiPath Test Automation using UiPath Test Suite series, part 3DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 3. In this session, we will cover desktop automation along with UI automation.
Topics covered:
UI automation Introduction,
UI automation Sample
Desktop automation flow
Pradeep Chinnala, Senior Consultant Automation Developer @WonderBotz and UiPath MVP
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Accelerate your Kubernetes clusters with Varnish CachingThijs Feryn
A presentation about the usage and availability of Varnish on Kubernetes. This talk explores the capabilities of Varnish caching and shows how to use the Varnish Helm chart to deploy it to Kubernetes.
This presentation was delivered at K8SUG Singapore. See https://feryn.eu/presentations/accelerate-your-kubernetes-clusters-with-varnish-caching-k8sug-singapore-28-2024 for more details.
LF Energy Webinar: Electrical Grid Modelling and Simulation Through PowSyBl -...DanBrown980551
Do you want to learn how to model and simulate an electrical network from scratch in under an hour?
Then welcome to this PowSyBl workshop, hosted by Rte, the French Transmission System Operator (TSO)!
During the webinar, you will discover the PowSyBl ecosystem as well as handle and study an electrical network through an interactive Python notebook.
PowSyBl is an open source project hosted by LF Energy, which offers a comprehensive set of features for electrical grid modelling and simulation. Among other advanced features, PowSyBl provides:
- A fully editable and extendable library for grid component modelling;
- Visualization tools to display your network;
- Grid simulation tools, such as power flows, security analyses (with or without remedial actions) and sensitivity analyses;
The framework is mostly written in Java, with a Python binding so that Python developers can access PowSyBl functionalities as well.
What you will learn during the webinar:
- For beginners: discover PowSyBl's functionalities through a quick general presentation and the notebook, without needing any expert coding skills;
- For advanced developers: master the skills to efficiently apply PowSyBl functionalities to your real-world scenarios.
Epistemic Interaction - tuning interfaces to provide information for AI supportAlan Dix
Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
2. CUTURE METHOD: All specimens should be inoculated onto a general purpose fungal medium fungi will grow very well on culture media used to isolate bacteria
3. Temperature: Most MOLDS grow best at: 25 – 30 OC Most YEAST grow best at 35 – 37 OC Most pathogenic fungi grow best: 30 to 32°C EXCEPT: Sporothrixschenckii : 25 to 27°C than 30°C
4. Incubation period: 14 days: general incubation period 7 days: to detect presence of yeast in the mouth, throat, or vagina 21 days: tissues and sterile body fluids other than blood 28 days: respiratory, bone marrow, blood specimens, and specimens in which dimorphic fungus are suspected Plates should be checked at least TWICE during the first week, when rapidly growing isolates may appear, weekly hereafter.
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8. CHROMagar A selective medium for the isolation and presumptive identification of yeast and filamentous fungi and differentiation of Candida albicans, C. tropicalis and C. krusei. Due to the differences in morphology and colors of the yeast colonies, this medium facilitates the detection of mixed yeast cultures in specimens. It may also be used as a selective isolation medium for other yeasts and for filamentous fungi instead of Sabouraud Dextrose Agar or similar media.
9. CHROMagar FORMULA IN GRAMS PER LITER Glucose ....................................... 20.00 Peptone …….................................. 10.00 Chloranphenicol ............................ 0.50 Chromogenic Mixture.............. ........ 0.40 Bacteriological Agar ....................... 15.00 Final pH 6.1 ± 0.2 at 25°C
10. CHROMagar Preparation Suspend 45.9 grams of the medium in one liter of distilled water. Mix well and heat with frequent agitation until complete dissolution. Distribute into adequate containers.
14. CHLAMYDOSPORE AGAR Used for differentiating Candida albicans from other species of Candida on the basis of chlamydospore formation. Candida albicans always form chlamydospore on this medium. The medium contains trypan blue to visualize the chlamydospore under microscopic evaluation. Biotin and polysaccharide are growth factors which stimulate chlamydospore formation. Potassium phosphate function as a buffer in the medium
17. LEVINE’S EMB AGAR For the isolation and differentiation of Escherichia coli and Enterobacter The dyes contained in this medium inhibit the growth of many accompanying Gram-positive microorganisms LEVINE EMB Agar can be used to identify Candida albicans in clinical specimens, if chlorotetracycline hydrochloride is added to inhibit the entire accompanying bacterial flora LEVINE EMB Agar can also be utilized for the identification of coagulase-positive staphylococci which grow characteristically as colorless "pin-point" colonies and which show good agreement with the results of the coagulase test
18. LEVINE’S EMB AGAR Typical Composition (g/liter) Peptone …………………………………………………………….10.0 Lactose ……………………………………………………………..10.0 Di-potassium hydrogen phosphate ………………………..2.0 Eosin, yellowish …………………………………………………..0.4 Methylene blue …………………………………………………...0.065 Agar-agar …………………………………………………………..13.5 If cultivating Candida, add 100 mg tetracycline hydrochloride/litre after autoclaving and mix homogeneously. The culture medium then is blue
19. LEVINE’S EMB AGAR To obtain a primary culture of Candida, incubate the plates containing chlorotetracycline in a 10 % carbon dioxide atmosphere Appearance: "Spidery" or "feathery“ - Candida albicans Yeast-like, round, smooth - Other Candida species; Sometimes Nocardia
20. SABHI AGAR Used for the cultivation of pathogenic and nonpathogenic fungi from a variety of clinical and nonclinical sources Sabouraud Dextrose Agar is a general purpose medium devised by Sabouraud for the cultivation of dermatophytes Brain Heart Infusion (BHI) Agar has proven to be effective in the cultivation of a wide variety of microorganisms and is recommended for the primary recovery of fungi from clinical specimens
21. SABHI AGAR SABHI Agar combines the ingredients of these two formulations to provide a medium which was found to yield greater recovery of pathogenic fungi than either medium individually It is recommended for the recovery of fungi from clinical specimens
22. SABHI AGAR Formulation: SABHI Agar contains two peptones and brain heart infusion solids as sources of amino acids, nitrogen, sulfur, carbon and trace ingredients Dextrose is an energy source for the metabolism of microorganisms. Sodium chloride provides essential electrolytes
24. Sabhi agar with Chloramphenicol and Cycloheximide Selective medium for use in the cultivation of pathogenic and nonpathogenic fungi from a variety of clinical and nonclinical sources Chloramphenicol Gram positive and Gram negative organisms Cycloheximide Most saprophytic molds
25. CZAPEK’S AGAR Czapek's Solution Agar is a synthetic medium widely used in mycological laboratories Many moulds produce very characteristic colonies on it and may also exude pigmented substances Aerial growth is often suppressed and sporulation may be enhanced Some moulds, however, grow poorly on this medium and may even fail to sporulate altogether, often because of their inability to synthesize vitamins As noted above, the addition of agar to this medium makes it, in reality, a semi-synthetic one