This Advanced Haematology course focuses on various anemia types, Leukemia, hematological disorders, chromosomal studies related to these disorders, and the use of radioisotopes in haematology. The course also addresses the importance of safety measures regarding radiation hazards and the disposal of radioactive materials. Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com

1. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Program Name: B.Sc. Medical Lab Technology
Syllabus of Advanced Haematology
Course Name: Advanced Haematology Course Type
Course Coordinator: Attuluri Vamsi Kumar – B.Sc. MLT, M.Sc. MLT, PhD (Perusing)
Designation: Assistant Professor
Department: Department of Medical Lab Technology (MLT)
Program Core
PRE-REQUISITE Applied Haematology – II Sem: 6 S. No: 40
About Vamsi: I am academician in Medical Laboratory Sciences with a strong desire to improve Outcome
based education (OBE) structured MLT education. I am constantly focusing on building an academic
atmosphere that is set high standards with strong multi blended teaching pedagogy models.
YouTube: https://www.youtube.com/@vamsiMLT
SlideShare: https://www.slideshare.net/VamsiIntellectual
Website: www.mltmaster.com / https://sites.google.com/view/vamsi-intellectual-protfolio/home
LinkedIn: https://www.linkedin.com/in/vamsi-kumar-attuluri-ab8987128/?originalSubdomain=in
Research Gate: https://www.researchgate.net/profile/Attuluri-Kumar
Orcid ID: https://orcid.org/0000-0001-9278-6714
Contact No: +91 7416660584
Mail ID: vamsifmlt@gmail.com
A. COURSE DESCRIPTION
This Advanced Haematology course focuses on various anemia types, Leukemia,
hematological disorders, chromosomal studies related to these disorders, and the use of
radioisotopes in haematology. The course also addresses the importance of safety measures
regarding radiation hazards and the disposal of radioactive materials.
B. COURSE OBJECTIVES:
1. To understand and explain (K1) the laboratory diagnosis and classification of different types of
anemia.
2. To analyze (K4) the definition, classification, and laboratory diagnosis of Leukemia.
3. To evaluate (K5) and implement (K3) laboratory techniques for diagnosing bleeding disorders,
including Hemophilia A, B & Von-Willebrand disease, DIC, and Platelet disorders.
4. To apply (K3) knowledge of chromosomal studies in hematological disorders and understand their
significance.
5. To create (K6) safety protocols for the use of radioisotopes in haematology, addressing radiation
hazards, and disposing of radioactive material effectively and safely.
C. COURSE OUTCOMES
CO
No
Statement Performance
Indicator
Level of
Learning
(Highest BT
Level)
Target
Attainment
CO1 Students will remember (K1) the basic definitions,
classifications, and laboratory diagnosis techniques
of various types of anemia and Leukemia.
PI 2.5, PI 6 K1:
Remembering
70%
CO2 Students will understand (K2) the principles behind
the laboratory diagnosis of bleeding disorders
including Hemophilia A, B & Von-Willebrand
disease, DIC, and Platelet disorders.
PI 2.2, PI 2.4,
PI 2.5
K2:
Understanding
70%
CO3 Students will be able to apply (K3) the learned
knowledge in conducting chromosomal studies in
PI 2.5, PI 6 K3: Applying 70%

2. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
hematological disorders and appreciate their
significance.
CO4 Students will analyze (K4) the use of radioisotopes
in hematological studies and their effects on blood
volume, red cell volume, red cell life span, and
platelet life span.
PI 2.3, PI 2.5,
PI 2.7
K4: Analyzing 70%
CO5 Students will create (K5) safe and effective
laboratory procedures for handling and disposing of
radioactive materials.
PI 2.2, PI 2.3,
PI 4
K5: Creating 70%
CO6 Students will evaluate (K6) radiation hazards and
propose preventive measures based on the
knowledge gained throughout the course.
PI 4, PI 7, PI 8 K6: Evaluating 70%
D. SYLLABUS
Unit-1 Anemia and Leukemia Contact Hours:15
Chapter 1.1 Laboratory Diagnosis and Classification of Anemia- Laboratory diagnosis of Iron
deficiency anemia, Laboratory diagnosis of Megaloblastic anemia & Pernicious anemia,
Classification and Laboratory diagnosis of Hemolytic anemia
Chapter 1.2 Overview of Leukemia- Definition, classification and laboratory diagnosis of Leukemia
Experiment 1 /
Case study
Study and interpretation of Histogram of Automated Blood cell counter
Experiment 2 /
Case study
To estimate serum iron and total iron binding capacity
Experiment 3 /
Case study
Screening tests for enzymes deficiency: Pyruvate Kinase, G6PD
Unit-2 Hematological Disorders and Chromosomal Studies Contact Hours:15
Chapter 2.1 Laboratory Diagnosis of Bleeding Disorders - 40
Chapter 2.2 Chromosomal Studies and Their Significance in Hematological Disorders- Chromosomal
studies in various hematological disorders and their significance, Laboratory approach for
investigating thrombosis
Experiment 4 /
Case study
To estimate Hb-F, Hb-A2 in a given blood sample
Experiment 5 /
Case study
To estimate plasma and urine Hemoglobin in the given specimens
Experiment 6 /
Case study
To demonstrate the presence of Hb-S by Sickling and Solubility tests
Experiment 7 /
Case study
Perform Hb electrophoresis (alkaline)
Unit-3 Use of Radioisotopes and Safety Measures Contact Hours:15
Chapter 3.1 Use of Radioisotopes in Hematology- Using radioisotopes measurement of Blood volume,
Determination of Red cell volume and Plasma volume, Red cell life span, Platelet life span
Chapter 3.2 Radiation Hazards and Disposal of Radioactive Material- Radiation hazards and its
prevention, Disposal of radioactive material
Experiment 8 /
Case study
Perform osmotic red cell fragility,
Estimation of Protein C, S
Experiment 9 /
Case study
Detection of Fibrin degradation products (FDPs)
Experiment 10 /
Case study
To perform various platelet function tests such as whole blood clot retraction test, prothrombin
consumption index (PCI) Platelet adhesion, aggregation and PF3 availability test,
Peripheral Blood Lymphocyte Culture for chromosome studies in Leukemia.
Self-study topics for Advance learners: Advanced Techniques in Hematological Diagnostics:
This topic will delve deeper into the newest techniques, technologies, and methodologies that are being

3. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
utilized in hematology labs across the globe., Genomics in Hematology: This topic could explore how
genetic studies and advancements in genomic technologies are shaping our understanding and
approach to hematological disorders., Role of AI and Machine Learning in Hematology: This topic
would focus on the application of AI in hematological diagnostics and treatment. It could include
aspects such as automated microscopy, prediction models, and AI-driven diagnostic tools.,
Hematopoietic Stem Cells and Regenerative Medicine: Advanced learners could study the potential
applications of hematopoietic stem cells in the treatment of various hematological disorders and their
role in regenerative medicine.
E. TEXT BOOKS/REFERENCE BOOKS
TEXT BOOKS
T1 "Hoffman Hematology: Basic Principles and Practice" by Ronald Hoffman, Edward J.
Benz, Jr., Leslie E. Silberstein, Helen Heslop, Jeffrey Weitz, John Anastasi, Mohamed Salama,
and Paul A. M. H. H. Schiff
T2 "Wintrobe's Clinical Hematology" by John P. Greer, Daniel A. Arber, Bertil Glader, Alan
F. List, Robert T. Means Jr., Frixos Paraskevas, George M. Rodgers
T3 "Rodak's Hematology: Clinical Principles and Applications" by Elaine Keohane, Catherine
N. Otto, Jeanine Walenga
REFERENCE BOOKS
R1 "Hematology in Clinical Practice" by Robert S. Hillman, Kenneth A. Ault, Henry M.
Rinder
R2 "Williams Hematology" by Kenneth Kaushansky, Marshall A. Lichtman, Josef Prchal,
Marcel M. Levi, Oliver W. Press, Linda J. Burns, Michael Caligiuri
R3 "Clinical Hematology Atlas" by Bernadette F. Rodak, Jacqueline H. Carr

4. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Code Performance Indicators
PI 1 Demonstrate professional interpersonal, oral, and written communications skills sufficient to
serve the needs of patients and the public including an awareness of how diversity may affect
the communication process.
PI 2 Perform pre-analytical, analytical, and post-analytical processes:
PI 2.1 Demonstrate ability to understand investigation/test requisition.
PI 2.2 Collecting the relevant clinical samples along with complete and accurate documentation with
proper safety measures in relation to sample accountability.
PI 2.3 To transport the samples with precautionary measures to the relevant lab section.
PI 2.4 Demonstrate the ability to prepare clinical sample for processing
PI 2.5 To demonstrate the knowledge of accurate sample processing for the required lab
investigation. Perform routine clinical laboratory tests in clinical chemistry,
haematology/haemostasis, immunology, immunohematology, microbiology, Histopathology,
Cytopathology, body fluid analysis, and laboratory operations.
PI 2.6 Perform mathematical calculations related to all areas of the clinical laboratory
PI 2.7 Ability to record the test results/data.
PI 2.8 To demonstrate the ability to interpret the test reports and its documentation in lab records.
PI 2.9 Demonstrate ability to release the report to the right person in minimum turn-around time
(TAT).
PI 3 Perform problem solving and troubleshooting techniques for laboratory methodologies
Correlate laboratory test results with patient diagnosis and treatment.
PI 4 To follow basic quality assessment protocol of clinical laboratory.
PI 5 Demonstrate routine laboratory techniques sufficient to orient new employees within the
clinical laboratory.
PI 6 Apply basic scientific principles in learning new techniques/procedures; demonstrate
application of principles and methodologies.
PI 7 Utilize computer technology applications to interact with computerized instruments and
laboratory information systems.
PI 8 Demonstrate adequate knowledge of computer software as it applies to document production,
spreadsheets, and presentations.
PI 9 Demonstrate professional behaviour with co-team mates.
PI 10 Demonstrate sensitivity and compassion towards patients.