This course in Haematology equips students with a comprehensive understanding of blood, its cellular components, laboratory safety measures, hematological studies, and quality assurance in the field of Haematology. The course includes a theoretical exploration of various aspects of Haematology, as well as practical experiments to provide hands-on experience. Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com

1. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Program Name: B.Sc. Medical Lab Technology
Syllabus of Basic Haematology
Course Name: Basic Haematology Course Type
Course Coordinator: Attuluri Vamsi Kumar – B.Sc. MLT, M.Sc. MLT, PhD (Perusing)
Designation: Assistant Professor
Department: Department of Medical Lab Technology (MLT)
Program Core
PRE-REQUISITE Introduction to National Healthcare Delivery
System in India, Medical Terminology and
record keeping, Introduction to Quality and
Patient safety
Sem: 2 S. No: 12
About Vamsi: I am academician in Medical Laboratory Sciences with a strong desire to improve Outcome
based education (OBE) structured MLT education. I am constantly focusing on building an academic
atmosphere that is set high standards with strong multi blended teaching pedagogy models.
YouTube: https://www.youtube.com/@vamsiMLT
SlideShare: https://www.slideshare.net/VamsiIntellectual
Website: www.mltmaster.com / https://sites.google.com/view/vamsi-intellectual-protfolio/home
LinkedIn: https://www.linkedin.com/in/vamsi-kumar-attuluri-ab8987128/?originalSubdomain=in
Research Gate: https://www.researchgate.net/profile/Attuluri-Kumar
Orcid ID: https://orcid.org/0000-0001-9278-6714
Contact No: +91 7416660584
Mail ID: vamsifmlt@gmail.com
A. COURSE DESCRIPTION
This course in Haematology equips students with a comprehensive understanding of blood, its
cellular components, laboratory safety measures, hematological studies, and quality assurance
in the field of Haematology. The course includes a theoretical exploration of various aspects
of Haematology, as well as practical experiments to provide hands-on experience.
B. COURSE OBJECTIVES:
1. List and describe the important equipment used in a Haematology laboratory, recall the types of
anticoagulants and their mode of action, and identify normal blood cells.
2. Explain the composition and function of blood, understand the importance of Haematology, and
interpret the results of various hematological investigations.
3. Apply the knowledge of safety measures in a Haematology laboratory, demonstrate the correct
method of collecting and preserving blood samples, and conduct a urine analysis.
4. Analyze the synthesis and degradation process of Hemoglobin, differentiate between the formation
processes of various cellular components of blood (Erythropoiesis, Leucopoiesis,
Thrombopoiesis), and scrutinize urine analysis results.
5. Evaluate the importance of quality assurance in Haematology, assess the internal and external
quality control measures, and determine the accuracy and precision of hematological
investigations.
C. COURSE OUTCOMES
CO
No
Statement Performance
Indicator
Level of
Learning
(Highest BT
Level)
Target
Attainment
CO1 Students will be able to recall and define the core
concepts and terminology of Haematology,
PI 1, PI 6 K1:
Remembering
70%

2. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
including the key components of blood, types of
anticoagulants, and major hematological studies.
CO2 Students will understand the composition and
function of blood, the process of various
hematological studies, and the principles behind
laboratory safety measures.
PI 2.1, PI 2.3,
PI 2.5, PI 6, PI
8
K2:
Understanding
70%
CO3 Students will be able to apply their knowledge and
skills to carry out hematological experiments,
collect and preserve blood samples, and conduct
urine analyses.
PI 2.2, PI 2.4,
PI 2.5, PI 5, PI
6, PI 7
K3: Applying 70%
CO4 Students will be capable of analyzing the results of
hematological investigations, differentiating the
formation processes of cellular components of
blood, and understanding the synthesis and
degradation process of Hemoglobin.
PI 2.8, PI 2.9,
PI 3, PI 6
K4: Analyzing 70%
CO5 Students will be proficient in evaluating the
importance and effectiveness of quality assurance
in Haematology, assessing internal and external
quality control measures, and determining the
accuracy and precision of laboratory investigations.
PI 2.7, PI 2.9,
PI 4, PI 7
K5: Evaluating 70%
CO6 Students will develop the ability to create new
solutions and protocols to address challenges in the
field of Haematology, innovatively use laboratory
equipment for hematological investigations, and
propose improvements to existing quality assurance
proto
PI 2.9, PI 3, PI
4, PI 5, PI 6,
PI 7
K6: Creating 70%
D. SYLLABUS
Unit-1 Fundamentals of Haematology and Laboratory Practices Contact Hours:15
Chapter 1.1 Overview of Haematology and Lab Safety- Definition of Haematology, Importance of
Haematology, Important equipment used in Haematology, Laboratory organization, Safety
measures in the Haematology Laboratory
Chapter 1.2 Blood Composition and Role of Anticoagulants- Introduction to blood, its composition,
function, and normal cellular components, Anticoagulants: types, mode of action, and
preference of anticoagulants for different hematological studies, Collection and preservation of
blood sample for various hematological investigations
Experiment 1 /
Case study
Preparation of anticoagulants (EDTA, Sodium Citrate, Oxalate with Fluoride)
Experiment 2 /
Case study
Collection of blood sample for laboratory investigations
Experiment 3 /
Case study
Familiarization and usage of routine Haematology Lab instruments (Microscopes,
Hemocytometers)
Unit-2 Blood Components, Hemoglobin, and Cellular Formation Contact Hours:15
Chapter 2.1 Blood Cellular Components Formation and Hemoglobin - 12
Chapter 2.2 Morphology of Blood Cells and Hemostasis- Morphology of normal blood cells, Normal
Hemostasis & physiological properties of coagulation factors
Experiment 4 /
Case study
Identification of normal blood cells
Experiment 5 /
Case study
Study of Hemostasis and coagulation factors
Experiment 6 /
Case study
Hemoglobin analysis: structure, synthesis, and degradation
Experiment 7 / Studying Erythropoiesis, Leucopoiesis, and Thrombopoiesis

3. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Case study
Unit-3 Radioactivity, Urine Analysis, and Quality Assurance in
Haematology
Contact Hours:15
Chapter 3.1 Basics of Radioactivity and Urine Analysis- Radioactivity: definition, half-life, physical
decay, and units, Urine analysis
Chapter 3.2 Quality Assurance in Haematology- Internal and external quality control including reference
preparation, Routine quality assurance protocol, Statistical analysis i.e., Standard deviation,
Co-efficient of variation, accuracy, and precision
Experiment 8 /
Case study
Study of Radioactivity: understanding half-life, physical decay, and units
Experiment 9 /
Case study
Conducting Urine Analysis: routine biochemistry of Urine (pH, Specific Gravity, Glucose,
Ketones, Bilirubin, Albumin) and microscopic examination of urine
Experiment 10 /
Case study
Conducting Quality Assurance protocols in Haematology
Self-study topics for Advance learners: intricacies of blood coagulation, the disorders that can
occur, and the impact these can have on health and disease.Molecular Hematology: This topic can
include advanced understanding of the molecular basis of blood diseases including genetic
disorders.Flow Cytometry in Hematology: An introduction to this key technique used for measuring
and analyzing cells. This can include how it's used in both research and clinical
settings.Hematopathology: This field involves the study and diagnosis of diseases of the blood, bone
marrow, vascular system, and lymph nodes. Advanced learners can look into the pathology of blood
cells and blood diseases, including malignancies.Immunohematology: This subject would delve deeper
into the study of the reactions that take place between antigens present on blood cells and antibodies
present in plasma.
E. TEXT BOOKS/REFERENCE BOOKS
TEXT BOOKS
T1 Textbook of Medical Laboratory Technology by Praful B. Godkar
T2 Medical Laboratory Technology: A Procedure Manual for Routine Diagnostic Tests
Volume 1 by K. L. Mukherjee
T3 De Gruchy's Clinical Haematology in Medical Practice by Frank Firkin, J. Whitby, and J.
Lukens
REFERENCE BOOKS
R1 Practical Haematology by Barbara J. Bain, Imelda Bates, and Mike A. Laffan
R2 Clinical Diagnosis and Management by Laboratory Methods by John Bernard Henry
R3 Atlas of Haematology by George A. McDonald

4. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Code Performance Indicators
PI 1 Demonstrate professional interpersonal, oral, and written communications skills sufficient to
serve the needs of patients and the public including an awareness of how diversity may affect
the communication process.
PI 2 Perform pre-analytical, analytical, and post-analytical processes:
PI 2.1 Demonstrate ability to understand investigation/test requisition.
PI 2.2 Collecting the relevant clinical samples along with complete and accurate documentation with
proper safety measures in relation to sample accountability.
PI 2.3 To transport the samples with precautionary measures to the relevant lab section.
PI 2.4 Demonstrate the ability to prepare clinical sample for processing
PI 2.5 To demonstrate the knowledge of accurate sample processing for the required lab
investigation. Perform routine clinical laboratory tests in clinical chemistry,
haematology/haemostasis, immunology, immunohematology, microbiology, Histopathology,
Cytopathology, body fluid analysis, and laboratory operations.
PI 2.6 Perform mathematical calculations related to all areas of the clinical laboratory
PI 2.7 Ability to record the test results/data.
PI 2.8 To demonstrate the ability to interpret the test reports and its documentation in lab records.
PI 2.9 Demonstrate ability to release the report to the right person in minimum turn-around time
(TAT).
PI 3 Perform problem solving and troubleshooting techniques for laboratory methodologies
Correlate laboratory test results with patient diagnosis and treatment.
PI 4 To follow basic quality assessment protocol of clinical laboratory.
PI 5 Demonstrate routine laboratory techniques sufficient to orient new employees within the
clinical laboratory.
PI 6 Apply basic scientific principles in learning new techniques/procedures; demonstrate
application of principles and methodologies.
PI 7 Utilize computer technology applications to interact with computerized instruments and
laboratory information systems.
PI 8 Demonstrate adequate knowledge of computer software as it applies to document production,
spreadsheets, and presentations.
PI 9 Demonstrate professional behaviour with co-team mates.
PI 10 Demonstrate sensitivity and compassion towards patients.