This course provides a comprehensive exploration of stem cell biology, technology, and their applications in the field of medicine. The course delves into the foundations of stem cell biology, the role of stem cells in regenerative medicine, techniques and applications of stem cell technology, and the ethical, legal, and social implications of stem cell research. Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com

1. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Program Name: B.Sc. Medical Lab Technology
Syllabus of Program Elective course - II (Stem cell technology)
Course Name: Program Elective course - II (Stem cell technology) Course Type
Course Coordinator: Attuluri Vamsi Kumar – B.Sc. MLT, M.Sc. MLT, PhD (Perusing)
Designation: Assistant Professor
Department: Department of Medical Lab Technology (MLT)
Program
Elective
PRE-REQUISITE Program Elective -I Sem: 4 S. No: 29
About Vamsi: I am academician in Medical Laboratory Sciences with a strong desire to improve Outcome
based education (OBE) structured MLT education. I am constantly focusing on building an academic
atmosphere that is set high standards with strong multi blended teaching pedagogy models.
YouTube: https://www.youtube.com/@vamsiMLT
SlideShare: https://www.slideshare.net/VamsiIntellectual
Website: www.mltmaster.com / https://sites.google.com/view/vamsi-intellectual-protfolio/home
LinkedIn: https://www.linkedin.com/in/vamsi-kumar-attuluri-ab8987128/?originalSubdomain=in
Research Gate: https://www.researchgate.net/profile/Attuluri-Kumar
Orcid ID: https://orcid.org/0000-0001-9278-6714
Contact No: +91 7416660584
Mail ID: vamsifmlt@gmail.com
A. COURSE DESCRIPTION
This course provides a comprehensive exploration of stem cell biology, technology, and their
applications in the field of medicine. The course delves into the foundations of stem cell
biology, the role of stem cells in regenerative medicine, techniques and applications of stem
cell technology, and the ethical, legal, and social implications of stem cell research.
B. COURSE OBJECTIVES:
1. Define and explain the types of stem cells, their maintenance, and differentiation pathways.
2. Describe the applications of stem cells in disease modeling, drug discovery, gene therapy, organ
transplantation, and personalized medicine.
3. Apply stem cell culture techniques, cell reprogramming, and genetic manipulation practices in
hypothetical scenarios.
4. Analyze case studies related to stem cell research and technology to understand the practical
implications and challenges in the field.
5. Evaluate the ethical, legal, and social implications of stem cell research and discuss potential
solutions to these issues.
C. COURSE OUTCOMES
CO
No
Statement Performance
Indicator
Level of
Learning
(Highest BT
Level)
Target
Attainment
CO1 Identify the types of stem cells, their unique
characteristics, and the key techniques used in stem
cell technology.
PI 2.5, PI 6 Knowledge (K1) 70%
CO2 Understand the role of stem cells in regenerative
medicine and their applications in disease modeling,
drug discovery, gene therapy, organ transplantation,
and personalized medicine.
PI 2.1, PI 2.8,
PI 3
Comprehension
(K2)
70%
CO3 Demonstrate the ability to apply knowledge of stem
cell culture techniques, cell reprogramming, and
genetic manipulation to practical scenarios.
PI 2.5, PI 2.7,
PI 5, PI 6
Application
(K3)
70%

2. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
CO4 Analyze real-world case studies in stem cell
research and technology, identifying key
implications, challenges, and potential solutions.
PI 1, PI 2.8, PI
3, PI 7, PI 8
Analysis (K4) 70%
CO5 Formulate research strategies to address current
challenges in stem cell technology, incorporating
considerations for ethical, legal, and social
implications.
PI 2.8, PI 3, PI
4, PI 7, PI 8
Synthesis (K5) 70%
CO6 Critically assess the potential impacts of emerging
trends and technologies in stem cell research and
their role in shaping future healthcare outcomes.
PI 1, PI 2.8, PI
3, PI 4, PI 7,
PI 8
Evaluation (K6) 70%
D. SYLLABUS
Unit-1 Introduction to Stem Cell Biology and Regenerative
Medicine
Contact Hours:15
Chapter 1.1 Basic Stem Cell Biology- Introduction to stem cells, Types of stem cells (Embryonic, Adult,
and Induced Pluripotent Stem Cells), Stem cell niches, Potency and differentiation, Stem cell
maintenance, Stem cell markers
Chapter 1.2 Regenerative Medicine: Basics and Application- Introduction to regenerative medicine, Stem
cells in regenerative medicine, Tissue engineering, Scaffolds for tissue engineering, Organ
regeneration, Healing and repair
Experiment 1 /
Case study
Case study:Differentiating Stem Cells: A Case of Cellular Identity Crisis
Experiment 2 /
Case study
Case study: The Use of Stem Cells in Parkinson's Disease Treatment
Experiment 3 /
Case study
Case study:Stem Cells in Burn Wound Healing
Unit-2 Stem Cell Technology and Its Applications Contact Hours:15
Chapter 2.1 Techniques in Stem Cell Technology - 29
Chapter 2.2 Applications of Stem Cell Technology- Stem cells in disease modelling, Drug discovery and
toxicity testing, Stem cells in gene therapy, Stem cells in organ transplantation, Personalized
medicine, Future directions of stem cell technology
Experiment 4 /
Case study
Case study: Creation of the First iPSCs: A Breakthrough in Regenerative Medicine
Experiment 5 /
Case study
Case study: Reprogramming Adult Cells: A Case of Induced Pluripotency
Experiment 6 /
Case study
Case study: Cloning Dolly the Sheep: Understanding Somatic Cell Nuclear Transfer
Experiment 7 /
Case study
Case study: Gene Editing in Stem Cells: The Case of CRISPR-Cas9
Unit-3 Ethical, Legal, and Social Implications of Stem Cell
Research
Contact Hours:15
Chapter 3.1 Ethical Issues in Stem Cell Research- Ethical considerations in embryonic stem cell research,
Informed consent in stem cell research, Ownership and patenting issues, Research integrity
and misconduct, Controversies and public opinion
Chapter 3.2 Legal and Social Implications of Stem Cell Research- Regulation of stem cell research –
global perspectives, The role of the FDA and other regulatory bodies, Implications for
healthcare policy, Social implications of stem cell technology, Future of stem cell research and
therapy
Experiment 8 /
Case study
Case study: The Hwang Woo-Suk Scandal: A Case of Research Misconduct
Experiment 9 /
Case study
Case study: The Debate Over Embryonic Stem Cell Research: Ethical Considerations
Experiment 10 / Case study: Patent Wars: The Battle Over Stem Cell Technologies

3. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Case study
Self-study topics for Advance learners: Advanced Cellular Reprogramming: Advanced learners
can explore the mechanisms of pluripotency induction and maintenance, as well as cutting-edge
techniques for somatic cell reprogramming. Topics might include mRNA-based reprogramming, small
molecule-induced reprogramming, and transdifferentiation., Regenerative Medicine and Translational
Applications: This topic covers the current state of regenerative medicine using stem cells, including
tissue engineering and organ regeneration. Case studies could include specific diseases or conditions
and the potential or realized benefits of stem cell therapies., Gene Editing in Stem Cells: Here, students
can delve into advanced gene editing techniques such as CRISPR-Cas9, understanding its application
in stem cell research for the generation of disease models or therapeutic purposes., Stem Cell Niches
and Microenvironment: This involves an in-depth study of the microenvironment's role in regulating
stem cell behavior, including cell-to-cell interactions and the impact of the extracellular matrix.
E. TEXT BOOKS/REFERENCE BOOKS
TEXT BOOKS
T1 "Essentials of Stem Cell Biology" by Robert Lanza, Anthony Atala
T2 "Human Stem Cell Manual: A Laboratory Guide" by Jeanne F. Loring, Robin L.
Wesselschmidt, Philip H. Schwartz
T3 "Stem Cells: From Basic Research to Therapy, Volume 1: Basic Stem Cell Biology, Tissue
Formation during Development, and Model Organisms" by Federico Calegari, Claudia
Waskow
REFERENCE BOOKS
R1 "Stem Cells: A Very Short Introduction" by Jonathan Slack
R2 "The Science of Stem Cells" by Jonathan M. W. Slack
R3 "Stem Cells in Regenerative Medicine: Science, Regulation and Business Strategies" by
Alain A. Vertes, Nasib Qureshi, Arnold I. Caplan, Lee E. Babiss

4. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Code Performance Indicators
PI 1 Demonstrate professional interpersonal, oral, and written communications skills sufficient to
serve the needs of patients and the public including an awareness of how diversity may affect
the communication process.
PI 2 Perform pre-analytical, analytical, and post-analytical processes:
PI 2.1 Demonstrate ability to understand investigation/test requisition.
PI 2.2 Collecting the relevant clinical samples along with complete and accurate documentation with
proper safety measures in relation to sample accountability.
PI 2.3 To transport the samples with precautionary measures to the relevant lab section.
PI 2.4 Demonstrate the ability to prepare clinical sample for processing
PI 2.5 To demonstrate the knowledge of accurate sample processing for the required lab
investigation. Perform routine clinical laboratory tests in clinical chemistry,
haematology/haemostasis, immunology, immunohematology, microbiology, Histopathology,
Cytopathology, body fluid analysis, and laboratory operations.
PI 2.6 Perform mathematical calculations related to all areas of the clinical laboratory
PI 2.7 Ability to record the test results/data.
PI 2.8 To demonstrate the ability to interpret the test reports and its documentation in lab records.
PI 2.9 Demonstrate ability to release the report to the right person in minimum turn-around time
(TAT).
PI 3 Perform problem solving and troubleshooting techniques for laboratory methodologies
Correlate laboratory test results with patient diagnosis and treatment.
PI 4 To follow basic quality assessment protocol of clinical laboratory.
PI 5 Demonstrate routine laboratory techniques sufficient to orient new employees within the
clinical laboratory.
PI 6 Apply basic scientific principles in learning new techniques/procedures; demonstrate
application of principles and methodologies.
PI 7 Utilize computer technology applications to interact with computerized instruments and
laboratory information systems.
PI 8 Demonstrate adequate knowledge of computer software as it applies to document production,
spreadsheets, and presentations.
PI 9 Demonstrate professional behaviour with co-team mates.
PI 10 Demonstrate sensitivity and compassion towards patients.