This course aims to provide students with an in-depth understanding of Immunopathology and Molecular Biology, with a focus on the immune system's role in health and disease, transplantation immunology, hypersensitivity, autoimmunity, and immune tolerance. Additionally, it introduces molecular biology, DNA structure, and replication, with practical applications of molecular techniques such as PCR, gel electrophoresis, and western blotting. Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com

1. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Program Name: B.Sc. Medical Lab Technology
Syllabus of Immunopathology and Molecular Biology
Course Name: Immunopathology and Molecular Biology Course Type
Course Coordinator: Attuluri Vamsi Kumar – B.Sc. MLT, M.Sc. MLT, PhD (Perusing)
Designation: Assistant Professor
Department: Department of Medical Lab Technology (MLT)
Program Core
PRE-REQUISITE Cytopathology Sem: 7 S. No: 49
About Vamsi: I am academician in Medical Laboratory Sciences with a strong desire to improve Outcome
based education (OBE) structured MLT education. I am constantly focusing on building an academic
atmosphere that is set high standards with strong multi blended teaching pedagogy models.
YouTube: https://www.youtube.com/@vamsiMLT
SlideShare: https://www.slideshare.net/VamsiIntellectual
Website: www.mltmaster.com / https://sites.google.com/view/vamsi-intellectual-protfolio/home
LinkedIn: https://www.linkedin.com/in/vamsi-kumar-attuluri-ab8987128/?originalSubdomain=in
Research Gate: https://www.researchgate.net/profile/Attuluri-Kumar
Orcid ID: https://orcid.org/0000-0001-9278-6714
Contact No: +91 7416660584
Mail ID: vamsifmlt@gmail.com
A. COURSE DESCRIPTION
This course aims to provide students with an in-depth understanding of Immunopathology and
Molecular Biology, with a focus on the immune system's role in health and disease,
transplantation immunology, hypersensitivity, autoimmunity, and immune tolerance.
Additionally, it introduces molecular biology, DNA structure, and replication, with practical
applications of molecular techniques such as PCR, gel electrophoresis, and western blotting.
B. COURSE OBJECTIVES:
1. Describe the basic concepts of immunology including the immune system's cellular components,
lymphoid organs, antigen-antibody reactions, and transplant immunology.
2. Explain the mechanisms of immune response, hypersensitivity, and autoimmunity and the role of
immune tolerance in health and disease.
3. Conduct immunology and molecular biology experiments, demonstrating proficiency in
techniques such as gradient centrifugation, immunofluorescence, electrophoresis, and PCR.
4. Analyze clinical samples to identify markers of immune disease, perform HLA typing and cross-
matching, and understand the implications of these results for patient care.
5. Synthesize knowledge from various areas of immunopathology and molecular biology to interpret
experimental results, formulate hypotheses, and propose future experiments.
C. COURSE OUTCOMES
CO
No
Statement Performance
Indicator
Level of
Learning
(Highest BT
Level)
Target
Attainment
CO1 Students will be able to identify and define the core
concepts and principles in immunology and
molecular biology, including the structure and
functions of immune system components and DNA
molecules.
PI 6 K1: Knowledge 70%
CO2 Students will demonstrate an understanding of the
mechanisms and dynamics of immune response,
PI 2.5 K2:
Comprehension
70%

2. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
hypersensitivity, autoimmunity, immune tolerance,
and DNA replication and repair.
CO3 Students will apply learned principles to carry out
essential techniques and procedures in immunology
and molecular biology, including PCR, gel
electrophoresis, and western blotting.
PI 2.2, PI 2.4,
PI 2.6
K3: Application 70%
CO4 Students will analyze and interpret results from
experimental procedures, making connections
between laboratory findings and clinical
implications.
PI 2.7, PI 2.8,
PI 3
K4: Analysis 70%
CO5 Students will integrate knowledge across different
topics in immunology and molecular biology to
design experiments and propose solutions to
problems in these fields.
PI 2.9, PI 6 K5: Synthesis 70%
CO6 Students will evaluate scientific literature in
immunology and molecular biology, critically
assess experimental designs and results, and make
evidence-based decisions.
PI 1, PI 4, PI 7,
PI 8
K6: Evaluation 70%
D. SYLLABUS
Unit-1 Immunology Basics and Immune Response Contact Hours:15
Chapter 1.1 Introduction to Immunology- Introduction to Immunology, Cells of the immune system,
Lymphoid organs of the Immune system
Chapter 1.2 Immune Responses and Transplant Immunology- Types and Mechanisms of immune
response, MHC I & II, HLA Typing & Cross matching, Transplant Immunology
Experiment 1 /
Case study
Anti- Nuclear Antibody (ANA)
Experiment 2 /
Case study
Anti- Neutrophil Cytoplasmic Antibody (ANCA)
Experiment 3 /
Case study
Rapid test for HBs Ag
Unit-2 Advanced Immunology and Introduction to Molecular
Biology
Contact Hours:15
Chapter 2.1 Hypersensitivity and Autoimmunity - 49
Chapter 2.2 Introduction to Molecular Biology- Introduction to Molecular Biology, Relationship of
Molecular Biology with other Sciences, Molecular Biology Techniques : Principle, Reagents
used, procedure and applications in Medical diagnostics
Experiment 4 /
Case study
AIDS Immunology and Pathogenesis (AIP)
Experiment 5 /
Case study
Thyroid Microsomal antigen (TMA)- Agglutination reactions
Experiment 6 /
Case study
Electrophoresis
Experiment 7 /
Case study
Gel diffusion
Unit-3 DNA Structure, Replication, and Molecular Biology
Techniques
Contact Hours:15
Chapter 3.1 DNA Structure and Replication- Chemical composition of DNA, DNA replication, DNA
damage and repair, Regulation of prokaryotic and eukaryotic gene expression
Chapter 3.2 Molecular Biology Techniques- Polymerase Chain Reaction and its advanced versions, Gel
electrophoresis, Western blotting, Cell Cycle
Experiment 8 / Nitro blue Tetrazolium Chloride Test (NBT)

3. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Case study
Experiment 9 /
Case study
FACS for CD4 and CD8
Experiment 10 /
Case study
ELISA for lab. diagnosis of AIDS, Polymerase Chain Reaction and its advanced versions<
Gel electrophoresis, Western blotting, Isolation of DNA and RNA,Estimation of DNA and
RNA, Determination of molecular weight and quantification of DNA using agarose gel
Self-study topics for Advance learners: Advanced Immunogenetics: Investigating the complex
genetic factors that contribute to the individual's immune response, including the role of minor
histocompatibility antigens in immune reactions.,In-depth Study of Immune Evasion Mechanisms:
Understanding the strategies used by various pathogens, such as viruses, bacteria, and parasites, to
evade the host's immune system, and how these strategies can inform the design of novel therapies.,
Immunotherapy in Disease Treatment: Studying the design and application of immunotherapies in the
treatment of diseases, such as cancer, autoimmune disorders, and infectious diseases., Emerging
Techniques in Molecular Biology: Learning about the latest advances in molecular biology techniques,
including CRISPR-Cas9 gene editing, next-generation sequencing, single-cell RNA sequencing, and
more., Computational Biology and Bioinformatics in Immunology and Molecular Biology: Applying
computational methods to analyze large-scale biological data and gain insights into immune responses,
gene expression, protein structure, and more., Epigenetic Control of Immune Responses:
Understanding the role of epigenetic mechanisms, such as DNA methylation and histone modification,
in regulating immune responses.
E. TEXT BOOKS/REFERENCE BOOKS
TEXT BOOKS
T1 Immunology by Ivan Roitt, Jonathan Brostoff, and David Male by
T2 Immunology by Thomas J. Kindt, Barbara A. Osborne, Richard Goldsby by
T3 Essential Immunology by Peter Delves, Seamus Martin, Dennis Burton, Ivan M. Roitt by
REFERENCE BOOKS
R1 Molecular Biology of the Gene by James D. Watson, Tania A. Baker, Stephen P. Bell,
Alexander Gann, Michael Levine, Richard Losick by
R2 Molecular Cell Biology by Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger,
Anthony Bretscher, Hidde Ploegh, Angelika Amon, Kelsey C. Martin by
R3 Medical Immunology by Daniel P Stites, Abba I Terr, Tristram G Parslow by

4. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Code Performance Indicators
PI 1 Demonstrate professional interpersonal, oral, and written communications skills sufficient to
serve the needs of patients and the public including an awareness of how diversity may affect
the communication process.
PI 2 Perform pre-analytical, analytical, and post-analytical processes:
PI 2.1 Demonstrate ability to understand investigation/test requisition.
PI 2.2 Collecting the relevant clinical samples along with complete and accurate documentation with
proper safety measures in relation to sample accountability.
PI 2.3 To transport the samples with precautionary measures to the relevant lab section.
PI 2.4 Demonstrate the ability to prepare clinical sample for processing
PI 2.5 To demonstrate the knowledge of accurate sample processing for the required lab
investigation. Perform routine clinical laboratory tests in clinical chemistry,
haematology/haemostasis, immunology, immunohematology, microbiology, Histopathology,
Cytopathology, body fluid analysis, and laboratory operations.
PI 2.6 Perform mathematical calculations related to all areas of the clinical laboratory
PI 2.7 Ability to record the test results/data.
PI 2.8 To demonstrate the ability to interpret the test reports and its documentation in lab records.
PI 2.9 Demonstrate ability to release the report to the right person in minimum turn-around time
(TAT).
PI 3 Perform problem solving and troubleshooting techniques for laboratory methodologies
Correlate laboratory test results with patient diagnosis and treatment.
PI 4 To follow basic quality assessment protocol of clinical laboratory.
PI 5 Demonstrate routine laboratory techniques sufficient to orient new employees within the
clinical laboratory.
PI 6 Apply basic scientific principles in learning new techniques/procedures; demonstrate
application of principles and methodologies.
PI 7 Utilize computer technology applications to interact with computerized instruments and
laboratory information systems.
PI 8 Demonstrate adequate knowledge of computer software as it applies to document production,
spreadsheets, and presentations.
PI 9 Demonstrate professional behaviour with co-team mates.
PI 10 Demonstrate sensitivity and compassion towards patients.