This syllabus is designed for Applied Bacteriology and includes laboratory strategies for diagnosing various infectious syndromes, antibiotic susceptibility testing, advanced techniques in bacteriology, bacteriological examination of external elements, epidemiology, and microbe preservation. The learning objectives will be based on Bloom's Taxonomy and will require students to apply knowledge, understand concepts, analyze information, evaluate processes, and create solutions. Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com

1. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Program Name: B.Sc. Medical Lab Technology
Syllabus of Applied Bacteriology
Course Name: Applied Bacteriology Course Type
Course Coordinator: Attuluri Vamsi Kumar – B.Sc. MLT, M.Sc. MLT, PhD (Perusing)
Designation: Assistant Professor
Department: Department of Medical Lab Technology (MLT)
Program Core
PRE-REQUISITE Systematic Bacteriology Sem: 4 S. No: 25
About Vamsi: I am academician in Medical Laboratory Sciences with a strong desire to improve Outcome
based education (OBE) structured MLT education. I am constantly focusing on building an academic
atmosphere that is set high standards with strong multi blended teaching pedagogy models.
YouTube: https://www.youtube.com/@vamsiMLT
SlideShare: https://www.slideshare.net/VamsiIntellectual
Website: www.mltmaster.com / https://sites.google.com/view/vamsi-intellectual-protfolio/home
LinkedIn: https://www.linkedin.com/in/vamsi-kumar-attuluri-ab8987128/?originalSubdomain=in
Research Gate: https://www.researchgate.net/profile/Attuluri-Kumar
Orcid ID: https://orcid.org/0000-0001-9278-6714
Contact No: +91 7416660584
Mail ID: vamsifmlt@gmail.com
A. COURSE DESCRIPTION
This syllabus is designed for Applied Bacteriology and includes laboratory strategies for
diagnosing various infectious syndromes, antibiotic susceptibility testing, advanced
techniques in bacteriology, bacteriological examination of external elements, epidemiology,
and microbe preservation. The learning objectives will be based on Bloom's Taxonomy and
will require students to apply knowledge, understand concepts, analyze information, evaluate
processes, and create solutions.
B. COURSE OBJECTIVES:
1. List the various infectious syndromes and the laboratory strategies involved in their diagnosis
2. Explain the concept of antibiotic susceptibility testing and its various methods
3. Demonstrate the ability to conduct a bacteriological examination of water, milk, food, and air
4. Analyze the role of a microbiology laboratory in controlling nosocomial infections and in
implementing epidemiological markers
5. Evaluate different methods of microbe preservation, focusing on their principles and procedures
C. COURSE OUTCOMES
CO
No
Statement Performance
Indicator
Level of
Learning
(Highest BT
Level)
Target
Attainment
CO1 Students will be able to identify various infectious
syndromes and the laboratory strategies involved in
their diagnosis. They will also understand the
concept of antibiotic susceptibility and its various
methods
PI 1, PI 2.1, PI
2.5
K1 - Knowledge 70%
CO2 Students will comprehend and interpret the
procedures for conducting a bacteriological
examination of water, milk, food, and air. They will
also understand the methods used for sterility testing
of I/v fluids
PI 2.2, PI 2.3,
PI 2.4
K2 -
Comprehension
70%

2. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
CO3 Students will demonstrate the practical application
of advanced techniques in bacteriology, such as
nucleic acid techniques and automation in bacterial
culture detection and antimicrobial susceptibility
testing
PI 2.5, PI 2.6,
PI 3, PI 6
K3 -
Application
70%
CO4 Students will analyze and assess the role of a
microbiology laboratory in controlling nosocomial
infections and implementing epidemiological
markers
PI 4, PI 5, PI 9 K4 - Analysis 70%
CO5 Students will evaluate different methods of microbe
preservation, focusing on their principles,
procedures, and importance
PI 2.7, PI 2.8,
PI 2.9
K5 - Evaluation 70%
CO6 Students will synthesize their learning to conduct an
end-to-end study on a selected bacterial pathogen,
covering its diagnosis, antibiotic susceptibility,
handling external elements, its role in nosocomial
infections, and preservation methods
PI 7, PI 8, PI 9,
PI 10
K6 - Synthesis 70%
D. SYLLABUS
Unit-1 Clinical Diagnosis & Antibiotic Susceptibility Contact Hours:15
Chapter 1.1 Laboratory Strategy in Diagnosis of Infective Syndromes- Septicemia and bacteremia,
Upper Respiratory tract infections, Lower respiratory tract infections, Wound, skin, and deep
sepsis, Urinary tract infections, Genital Tract infections, Meningitis, Gastrointestinal
infections, Enteric fever, Tuberculosis (Pulm
Chapter 1.2 Antibiotic Susceptibility Testing in Bacteriology- Definition of antibiotics, Culture medium
used for Antibiotic susceptibility testing, Preparation and standardization of inoculum, Control
bacterial strains, Choice of antibiotics, MIC and MBC: Concepts and methods for
determination, Various methods of Antibiotic susceptibility testing with special reference to
Stokes and Kirby-Bauer method
Experiment 1 /
Case study
Inoculation of different culture media
Experiment 2 /
Case study
Isolation of pure cultures
Experiment 3 /
Case study
Processing of clinical samples for culture and identification of bacterial pathogens (Blood,
Throat swab, Sputum, Pus, Urine)
Unit-2 Advanced Techniques and Examination of External
Elements
Contact Hours:15
Chapter 2.1 Advanced Techniques in Bacteriology - 25
Chapter 2.2 Bacteriological Examination of External Elements- Examination of water, milk, food and
air, Sterility testing of I/v fluids, Introduction to nosocomial infections: sources, types, and
surveillance of hospital environment for microbial load
Experiment 4 /
Case study
Demonstration of PCR
Experiment 5 /
Case study
Demonstration of automation in bacterial culture detection and antimicrobial susceptibility
testing
Experiment 6 /
Case study
Antimicrobial susceptibility testing (Preparation and standardization of inoculum,
Demonstration of reference bacterial strains, Determine MIC and MBC of known bacteria
against a known antibiotic, Perform antibiotic susceptibility testing of clinical isol
Experiment 7 /
Case study
Collection, transportation and processing of water, milk, food, and air for bacteriological
examination
Unit-3 Epidemiology & Microbe Preservation Contact Hours:15

3. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Chapter 3.1 Epidemiology and Nosocomial Infections- Role of microbiology laboratory in control of
nosocomial infections, Introduction to epidemiological markers, Types of epidemiological
markers: Serotyping, Phage typing, Bacteriocin typing
Chapter 3.2 Preservation of Microbes- Basic concepts of preservation of microbes, Importance of
preserving bacteria, Principle and procedures of various short term and long term preservation
methods with special reference to Lyophilization
Experiment 8 /
Case study
Demonstrate sterility testing of intravenous fluid with positive and negative controls
Experiment 9 /
Case study
Demonstration of serotyping and bacteriocin typing
Experiment 10 /
Case study
Demonstration of lyophilization and other available preservation methods
Self-study topics for Advance learners: Advanced Diagnostic Technologies: Study of cutting-
edge diagnostic tools and methods, including microarray technology, next-generation sequencing, and
MALDI-TOF mass spectrometry in clinical microbiology., Molecular Diagnostics: Exploration of
techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing in the
identification of genetic diseases and infections.Lab Management and Informatics: Understanding of
Laboratory Information System (LIS), process management, quality control, and data analytics in the
lab setting.
E. TEXT BOOKS/REFERENCE BOOKS
TEXT BOOKS
T1 Clinical Laboratory Hematology (3rd Edition) by Shirlyn B. McKenzie and Lynne
Williams
T2 Tietz Textbook of Clinical Chemistry and Molecular Diagnostics by Carl A. Burtis,
Edward R. Ashwood, and David E. Bruns
T3 Medical Microbiology (8th Edition) by Patrick R. Murray, Ken S. Rosenthal, and Michael
A. Pfaller
REFERENCE BOOKS
R1 Clinical Immunology and Serology: A Laboratory Perspective (4th Edition) by Christine
Dorresteyn Stevens and Linda E. Miller
R2 Linne & Ringsrud's Clinical Laboratory Science: Concepts, Procedures, and Clinical
Applications by Mary Louise Turgeon
R3 Medical Laboratory Management and Supervision: Operations, Review, and Study Guide
by Lionel A. Varnadoe

4. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Code Performance Indicators
PI 1 Demonstrate professional interpersonal, oral, and written communications skills sufficient to
serve the needs of patients and the public including an awareness of how diversity may affect
the communication process.
PI 2 Perform pre-analytical, analytical, and post-analytical processes:
PI 2.1 Demonstrate ability to understand investigation/test requisition.
PI 2.2 Collecting the relevant clinical samples along with complete and accurate documentation with
proper safety measures in relation to sample accountability.
PI 2.3 To transport the samples with precautionary measures to the relevant lab section.
PI 2.4 Demonstrate the ability to prepare clinical sample for processing
PI 2.5 To demonstrate the knowledge of accurate sample processing for the required lab
investigation. Perform routine clinical laboratory tests in clinical chemistry,
haematology/haemostasis, immunology, immunohematology, microbiology, Histopathology,
Cytopathology, body fluid analysis, and laboratory operations.
PI 2.6 Perform mathematical calculations related to all areas of the clinical laboratory
PI 2.7 Ability to record the test results/data.
PI 2.8 To demonstrate the ability to interpret the test reports and its documentation in lab records.
PI 2.9 Demonstrate ability to release the report to the right person in minimum turn-around time
(TAT).
PI 3 Perform problem solving and troubleshooting techniques for laboratory methodologies
Correlate laboratory test results with patient diagnosis and treatment.
PI 4 To follow basic quality assessment protocol of clinical laboratory.
PI 5 Demonstrate routine laboratory techniques sufficient to orient new employees within the
clinical laboratory.
PI 6 Apply basic scientific principles in learning new techniques/procedures; demonstrate
application of principles and methodologies.
PI 7 Utilize computer technology applications to interact with computerized instruments and
laboratory information systems.
PI 8 Demonstrate adequate knowledge of computer software as it applies to document production,
spreadsheets, and presentations.
PI 9 Demonstrate professional behaviour with co-team mates.
PI 10 Demonstrate sensitivity and compassion towards patients.