This course, Applied Histopathology-I, intends to introduce students to the field of histotechnology, focusing on laboratory safety, handling and examination of tissue samples, fixation and decalcification procedures, as well as the techniques involved in tissue processing and staining. Practical application of theoretical knowledge will be encouraged through a range of lab experiments. Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com

1. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Program Name: B.Sc. Medical Lab Technology
Syllabus of Applied Histopathology – I
Course Name: Applied Histopathology – I Course Type
Course Coordinator: Attuluri Vamsi Kumar – B.Sc. MLT, M.Sc. MLT, PhD (Perusing)
Designation: Assistant Professor
Department: Department of Medical Lab Technology (MLT)
Program Core
PRE-REQUISITE Fundamentals of Histology Sem: 4 S. No: 28
About Vamsi: I am academician in Medical Laboratory Sciences with a strong desire to improve Outcome
based education (OBE) structured MLT education. I am constantly focusing on building an academic
atmosphere that is set high standards with strong multi blended teaching pedagogy models.
YouTube: https://www.youtube.com/@vamsiMLT
SlideShare: https://www.slideshare.net/VamsiIntellectual
Website: www.mltmaster.com / https://sites.google.com/view/vamsi-intellectual-protfolio/home
LinkedIn: https://www.linkedin.com/in/vamsi-kumar-attuluri-ab8987128/?originalSubdomain=in
Research Gate: https://www.researchgate.net/profile/Attuluri-Kumar
Orcid ID: https://orcid.org/0000-0001-9278-6714
Contact No: +91 7416660584
Mail ID: vamsifmlt@gmail.com
A. COURSE DESCRIPTION
This course, Applied Histopathology-I, intends to introduce students to the field of
histotechnology, focusing on laboratory safety, handling and examination of tissue samples,
fixation and decalcification procedures, as well as the techniques involved in tissue processing
and staining. Practical application of theoretical knowledge will be encouraged through a
range of lab experiments.
B. COURSE OBJECTIVES:
1. Define key terms and concepts related to histotechnology such as compound microscope, fixatives,
decalcification, tissue processing, and staining.
2. Explain the working principle and applications of various types of microscopes used in
histotechnology.
3. Demonstrate the ability to collect and transport specimens for histological examination following
standard safety protocols.
4. Compare and contrast different types of fixatives and their applications in a routine histopathology
laboratory.
5. Evaluate the effectiveness of different staining procedures for paraffin infiltrated and embedded
tissue.
C. COURSE OUTCOMES
CO
No
Statement Performance
Indicator
Level of
Learning
(Highest BT
Level)
Target
Attainment
CO1 Students will be able to recall and understand the
terminology used in histotechnology, including the
roles and maintenance of various instruments.
PI 1, PI 6 Remembering
(K1)
70%
CO2 Students will comprehend the operational
mechanisms of different types of microscopes and
their respective applications in histopathology.
PI 2.1, PI 2.2,
PI 2.3, PI 6
Understanding
(K2)
70%

2. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
CO3 Students will demonstrate practical skills in the
collection, transportation, fixation, and
decalcification of specimens for histological
examination, maintaining laboratory safety
standards.
PI 2.2, PI 2.3,
PI 2.4, PI 2.5,
PI 6, PI 7
Applying (K3) 70%
CO4 Students will differentiate between various types of
fixatives and their uses in a routine histopathology
laboratory and analyze the outcomes of different
fixation techniques.
PI 2.5, PI 2.6,
PI 2.7, PI 2.8,
PI 3
Analyzing (K4) 70%
CO5 Students will evaluate the effectiveness of different
staining procedures and discuss the application of
controls in staining procedures.
PI 3, PI 4 Evaluating (K5) 70%
CO6 Students will design and execute a sequence of
operations for processing tissues for histological
examination, which includes dehydration, clearing,
infiltration, and embedding, applying both manual
and automated tissue processing techniques.
PI 2.4, PI 2.5,
PI 2.6, PI 2.7,
PI 6, PI 7
Creating (K6) 70%
D. SYLLABUS
Unit-1 Introduction to Histopathology Contact Hours:15
Chapter 1.1 Foundations of Histotechnology- Introduction to Histotechnology
Compound microscope: Optical system, magnification and maintenance
Microscopy: Working principle, Applications of various types of microscopes i.e. dark field,
polarizing, phase contrast, interference, and fluorescent micro
Chapter 1.2 Laboratory Safety and Maintenance- Care and maintenance of laboratory equipment used in
histotechnology
Safety measures in a histopathology laboratory
Experiment 1 /
Case study
Demonstration of instruments used for dissection
Experiment 2 /
Case study
Use of antiseptics, disinfectants, and insecticides in a tissue culture processing laboratory
Experiment 3 /
Case study
Reception and labeling of histological specimens
Unit-2 Sample Collection, Fixation and Decalcification Contact Hours:15
Chapter 2.1 Handling and Examination of Tissue Samples - 28
Chapter 2.2 Fixatives and Decalcification- Various types of fixatives used in a routine histopathology
laboratory: Simple fixatives, Compound fixatives, Special fixatives for demonstration of
various tissue elements
Decalcification: Criteria of a good decalcification agent, Technique of decalcification, Various
types of decalcifying fluids
Experiment 4 /
Case study
Preparation of various fixatives: Helly’s fluid, Zenker’s fluid, Bouin’s fluid, Corney’s fluid,
10% Neutral formalin, Formal saline, Formal acetic acid, Pereyn’s fluid
Experiment 5 /
Case study
Testing of melting point of paraffin wax and perform embedding of given tissue in paraffin
block
Experiment 6 /
Case study
To process a bone for decalcification
Experiment 7 /
Case study
To prepare ascending and descending grades of alcohol from absolute alcohol
Unit-3 Tissue Processing and Staining Contact Hours:15
Chapter 3.1 Tissue Processing and Section Cutting- Processing of various tissues for histological
examination: Procedure, Components & principles of various types of automatic tissue
processors, Embedding

3. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Section Cutting: Introduction regarding equipment used for sectioning, Microtome Knives,
Sharpening of Microtome Knives, Freezing Microtome and various types of Cryostatsq
Chapter 3.2 Staining Techniques in Histopathology- Staining, Impregnation, and Mountants: Theory of
Staining, Classifications of Dyes, Preparation of Stains, Commonly used mountants in
histotechnology lab, General Staining Procedures, Nuclear Stains and Cytoplasmic stains
Routine Staining Procedures: Haematoxylin and Eosin Staining, Mallory's Phosphotungstic
Acid Haematoxylin (PTAH)
Experiment 8 /
Case study
Processing of tissue by manual and automated processor method
Experiment 9 /
Case study
To demonstrate various parts and types of microtome
Experiment 10 /
Case study
To perform section cutting (Rough and Fine), attachment of tissue sections to glass slides, and
using tissue floatation bath and drying of sections in oven (60-65C)
Self-study topics for Advance learners: Molecular Diagnostics: This field is rapidly growing
and provides advanced methods of detecting diseases at the genetic level., Advanced Hematology:
More in-depth look at diseases of the blood and blood-forming tissues, including coagulation disorders
and leukemias., Cytopathology: Detailed study of disease at the cellular level, including the use of
cytologic techniques in the diagnosis of cancer., Histopathology Techniques: Advanced techniques
used in the preparation and staining of tissue samples for microscopic examination., Toxicology and
Drug Monitoring: In-depth look at the principles of toxicology, common toxins, and the laboratory’s
role in monitoring therapeutic drug levels.
E. TEXT BOOKS/REFERENCE BOOKS
TEXT BOOKS
T1 "Medical Microbiology" by Patrick R. Murray, Ken S. Rosenthal, and Michael A. Pfaller
T2 "Tietz Textbook of Clinical Chemistry and Molecular Diagnostics" by Carl A. Burtis et al.
T3 "Rodak's Hematology: Clinical Principles and Applications" by Elaine Keohane et al.
REFERENCE BOOKS
R1 "Clinical Microbiology Made Ridiculously Simple" by Mark Gladwin and Bill Trattler
R2 "Clinical Chemistry: Principles, Techniques, and Correlations" by Michael L. Bishop et
al.
R3 "Hematology: Clinical Principles and Applications" by Bernadette Rodak et al.

4. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Code Performance Indicators
PI 1 Demonstrate professional interpersonal, oral, and written communications skills sufficient to
serve the needs of patients and the public including an awareness of how diversity may affect
the communication process.
PI 2 Perform pre-analytical, analytical, and post-analytical processes:
PI 2.1 Demonstrate ability to understand investigation/test requisition.
PI 2.2 Collecting the relevant clinical samples along with complete and accurate documentation with
proper safety measures in relation to sample accountability.
PI 2.3 To transport the samples with precautionary measures to the relevant lab section.
PI 2.4 Demonstrate the ability to prepare clinical sample for processing
PI 2.5 To demonstrate the knowledge of accurate sample processing for the required lab
investigation. Perform routine clinical laboratory tests in clinical chemistry,
haematology/haemostasis, immunology, immunohematology, microbiology, Histopathology,
Cytopathology, body fluid analysis, and laboratory operations.
PI 2.6 Perform mathematical calculations related to all areas of the clinical laboratory
PI 2.7 Ability to record the test results/data.
PI 2.8 To demonstrate the ability to interpret the test reports and its documentation in lab records.
PI 2.9 Demonstrate ability to release the report to the right person in minimum turn-around time
(TAT).
PI 3 Perform problem solving and troubleshooting techniques for laboratory methodologies
Correlate laboratory test results with patient diagnosis and treatment.
PI 4 To follow basic quality assessment protocol of clinical laboratory.
PI 5 Demonstrate routine laboratory techniques sufficient to orient new employees within the
clinical laboratory.
PI 6 Apply basic scientific principles in learning new techniques/procedures; demonstrate
application of principles and methodologies.
PI 7 Utilize computer technology applications to interact with computerized instruments and
laboratory information systems.
PI 8 Demonstrate adequate knowledge of computer software as it applies to document production,
spreadsheets, and presentations.
PI 9 Demonstrate professional behaviour with co-team mates.
PI 10 Demonstrate sensitivity and compassion towards patients.