This course in Analytical Clinical Biochemistry aims to provide students with a deep understanding of the principles, procedures, and techniques commonly used in the field. It covers spectrophotometry, colorimetry, photometry, various types of chromatography, and electrophoresis. Practical hands-on laboratory work complements the theoretical knowledge gained, enhancing students' comprehension and ability to apply these techniques. Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com

1. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Program Name: B.Sc. Medical Lab Technology
Syllabus of Analytical Clinical Biochemistry
Course Name: Analytical Clinical Biochemistry Course Type
Course Coordinator: Attuluri Vamsi Kumar – B.Sc. MLT, M.Sc. MLT, PhD (Perusing)
Designation: Assistant Professor
Department: Department of Medical Lab Technology (MLT)
Program Core
PRE-REQUISITE Biochemical metabolism Sem: 4 S. No: 27
About Vamsi: I am academician in Medical Laboratory Sciences with a strong desire to improve Outcome
based education (OBE) structured MLT education. I am constantly focusing on building an academic
atmosphere that is set high standards with strong multi blended teaching pedagogy models.
YouTube: https://www.youtube.com/@vamsiMLT
SlideShare: https://www.slideshare.net/VamsiIntellectual
Website: www.mltmaster.com / https://sites.google.com/view/vamsi-intellectual-protfolio/home
LinkedIn: https://www.linkedin.com/in/vamsi-kumar-attuluri-ab8987128/?originalSubdomain=in
Research Gate: https://www.researchgate.net/profile/Attuluri-Kumar
Orcid ID: https://orcid.org/0000-0001-9278-6714
Contact No: +91 7416660584
Mail ID: vamsifmlt@gmail.com
A. COURSE DESCRIPTION
This course in Analytical Clinical Biochemistry aims to provide students with a deep
understanding of the principles, procedures, and techniques commonly used in the field. It
covers spectrophotometry, colorimetry, photometry, various types of chromatography, and
electrophoresis. Practical hands-on laboratory work complements the theoretical knowledge
gained, enhancing students' comprehension and ability to apply these techniques.
B. COURSE OBJECTIVES:
1. Students should be able to remember and recall the theory behind different techniques such as
spectrophotometry, chromatography, and electrophoresis.
2. Students should be able to understand and explain the principles and applications of these
techniques.
3. Students should be able to apply these techniques in practical laboratory settings, following safety
protocols.
4. Students should be able to analyze results obtained from these techniques and understand their
implications.
5. Students should be able to evaluate and critique the efficacy of different techniques, as well as
their suitability for different analytical situations.
C. COURSE OUTCOMES
CO
No
Statement Performance
Indicator
Level of
Learning
(Highest BT
Level)
Target
Attainment
CO1 Students will be able to recall and identify the
principles, procedures, and techniques commonly
used in analytical biochemistry, such as
spectrophotometry, colorimetry, photometry, and
various types of chromatography, and
electrophoresis.
PI 2.5, PI 6 K1: Knowledge 70%

2. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
CO2 Students will demonstrate understanding of the
theoretical underpinnings of these techniques and
their diverse applications in the field of
biochemistry.
PI 2.5, PI 6, PI
7, PI 8
K2:
Comprehension
70%
CO3 Students will apply these techniques practically in
laboratory settings, demonstrating effective
laboratory skills and safety practices.
PI 2, PI 2.1, PI
2.2, PI 2.3, PI
2.4, PI 5
K3: Application 70%
CO4 Students will analyze the results derived from these
techniques, interpreting and understanding their
implications.
PI 2.7, PI 2.8 K4: Analysis 70%
CO5 Students will be able to evaluate the efficacy and
limitations of different analytical biochemistry
techniques, contributing to informed decision
making when presented with different analytical
challenges.
PI 2.9, PI 3, PI
4
K5: Evaluation 70%
CO6 Students will create detailed laboratory reports,
reflecting an integration of theory and practice, and
synthesizing the knowledge and skills acquired
throughout the course.
PI 1, PI 7, PI 8 K6: Synthesis 70%
D. SYLLABUS
Unit-1 Spectrophotometry, Colorimetry, and Photometry Contact Hours:15
Chapter 1.1 Spectrophotometry and Colorimetry- Introduction, Theory of spectrophotometry and
colorimetry, Lamberts law and Beers law, Applications of colorimetry and spectrophotometry
Chapter 1.2 Photometry- Introduction, General principles of flame photometry, Limitations of flame
photometry, Instrumentation, Applications of flame photometry, Atomic absorption
spectroscopy – Principle & applications
Experiment 1 /
Case study
Demonstration of the principle, working & maintenance of spectrophotometer.
Experiment 2 /
Case study
Demonstration of the principle, working & maintenance of colorimeter.
Experiment 3 /
Case study
Demonstration of the principle, working & maintenance of flame photometer.
Unit-2 Introduction to Chromatography Contact Hours:15
Chapter 2.1 Types of Chromatography and Applications - 27
Chapter 2.2 Advanced Chromatography Techniques- Thin layer chromatography: Introduction,
experimental techniques, application of TLC, limitations, High performance thin layer
chromatography, Column chromatography: Introduction, principle column efficiency,
application of column chromatography, Gas chromatography: Introduction principle,
instrumentation, application, Ion exchange chromatography: Introduction, Definition and
principle, cation and anion exchangers, application, Gel Chromatography: Introduction
Principle and method, application and advantages
Experiment 4 /
Case study
Demonstration of the principle, procedure of paper chromatography.
Experiment 5 /
Case study
Demonstration of the principle & procedure of Gas chromatography.
Experiment 6 /
Case study
Demonstration of the principle & demonstration of TLC.
Experiment 7 /
Case study
Demonstration of the principle & procedure of column chromatography.
Unit-3 Electrophoresis Contact Hours:15
Chapter 3.1 Basics of Electrophoresis- Introduction, Principle, Instrumentation, Applications
Chapter 3.2 Types of Electrophoresis- Paper electrophoresis, Gel electrophoresis

3. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Experiment 8 /
Case study
Demonstration of the principle & procedure of Electrophoresis.
Experiment 9 /
Case study
Experiment to compare Paper and Gel Electrophoresis.
Experiment 10 /
Case study
Detailed experiment involving Gel electrophoresis, focusing on technique and result
interpretation
Self-study topics for Advance learners: Advanced Spectroscopic Techniques: This could
include topics like Nuclear Magnetic Resonance (NMR) Spectroscopy, Mass Spectrometry, X-ray
crystallography, Fourier Transform Infrared (FTIR) Spectroscopy and their applications in clinical
biochemistry., Quantitative Proteomics and Genomics: Learn advanced techniques such as next-
generation sequencing, whole genome sequencing, transcriptomics, RNA-Seq, and proteomics.,
Bioinformatics in Clinical Biochemistry: How to use software and algorithms to interpret complex
biological data. This could also include principles of machine learning and artificial intelligence in
bioinformatics.
E. TEXT BOOKS/REFERENCE BOOKS
TEXT BOOKS
T1 Practical Clinical Biochemistry by Harold Varley
T2 Textbook of Medical Laboratory Technology by P.B. Godkar
T3 Medical Laboratory Technology by Kanai L. Mukherjee
REFERENCE BOOKS
R1 Principles of Biochemistry by M.A. Siddiqi
R2 Instrumental Analysis by Gurdeep R. Chatwal, Sham K. Anand
R3 Textbook of Medical Biochemistry by Rana Shinde, Chatterjee

4. B.Sc. MLT Syllabus
Attuluri Vamsi Kumar I Assistant professor I Dept of MLT I Ph No: 7416660584 I Website: mltmaster.com
Website: www.mltmaster.com I YouTube: https://www.youtube.com/@vamsiMLT
Code Performance Indicators
PI 1 Demonstrate professional interpersonal, oral, and written communications skills sufficient to
serve the needs of patients and the public including an awareness of how diversity may affect
the communication process.
PI 2 Perform pre-analytical, analytical, and post-analytical processes:
PI 2.1 Demonstrate ability to understand investigation/test requisition.
PI 2.2 Collecting the relevant clinical samples along with complete and accurate documentation with
proper safety measures in relation to sample accountability.
PI 2.3 To transport the samples with precautionary measures to the relevant lab section.
PI 2.4 Demonstrate the ability to prepare clinical sample for processing
PI 2.5 To demonstrate the knowledge of accurate sample processing for the required lab
investigation. Perform routine clinical laboratory tests in clinical chemistry,
haematology/haemostasis, immunology, immunohematology, microbiology, Histopathology,
Cytopathology, body fluid analysis, and laboratory operations.
PI 2.6 Perform mathematical calculations related to all areas of the clinical laboratory
PI 2.7 Ability to record the test results/data.
PI 2.8 To demonstrate the ability to interpret the test reports and its documentation in lab records.
PI 2.9 Demonstrate ability to release the report to the right person in minimum turn-around time
(TAT).
PI 3 Perform problem solving and troubleshooting techniques for laboratory methodologies
Correlate laboratory test results with patient diagnosis and treatment.
PI 4 To follow basic quality assessment protocol of clinical laboratory.
PI 5 Demonstrate routine laboratory techniques sufficient to orient new employees within the
clinical laboratory.
PI 6 Apply basic scientific principles in learning new techniques/procedures; demonstrate
application of principles and methodologies.
PI 7 Utilize computer technology applications to interact with computerized instruments and
laboratory information systems.
PI 8 Demonstrate adequate knowledge of computer software as it applies to document production,
spreadsheets, and presentations.
PI 9 Demonstrate professional behaviour with co-team mates.
PI 10 Demonstrate sensitivity and compassion towards patients.