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Vnti11 basics course

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- The document provides an overview of the modules in VectorNTI, including VectorNTI Explorer for storing data and VectorNTI for sequence analysis, design, and management. - It describes how to organize objects in VectorNTI Explorer using subsets, import/export objects, perform searches, and add new fields. - Steps are outlined for tasks like importing restriction enzymes, adding markers, selecting/exporting plasmids, and making subsets.

TechnologyBusiness
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basics course
Introduction ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Introduction ,[object Object],[object Object],[object Object],[object Object],[object Object]
Overview modules ,[object Object],[object Object],[object Object],[object Object]
VectorNTI Explorer
VectorNTI Explorer
VectorNTI Explorer New Object
VectorNTI Explorer ,[object Object],[object Object],New Subset
VectorNTI Explorer ,[object Object],separator Subset
VectorNTI Explorer New Object
VectorNTI Explorer New Object
VectorNTI Explorer New Object
VectorNTI Explorer New Object
VectorNTI Explorer New Object
VectorNTI Explorer
VectorNTI Explorer ,[object Object],[object Object]
VectorNTI Explorer ,[object Object],[object Object],[object Object]
VectorNTI Explorer ,[object Object],[object Object],[object Object],[object Object],[object Object]
VectorNTI Explorer ,[object Object],[object Object],[object Object],[object Object],[object Object]
VectorNTI Explorer ,[object Object],[object Object],[object Object],[object Object],[object Object]
VectorNTI Explorer subset selected molecule selected
VectorNTI Explorer
VectorNTI Explorer
VectorNTI Explorer We want to gather all NCBI molecules in a separate subset for easy access
VectorNTI Explorer
VectorNTI Explorer
VectorNTI Explorer
VectorNTI Explorer
VectorNTI Explorer
VectorNTI Explorer
VectorNTI Explorer
VectorNTI Explorer
VectorNTI Explorer Backup on other location e.g. network drive or external drive Local database on network drive » use same database on ≠ computers We want to add some more structured information to each entry -> new field!
VectorNTI Explorer New field Import fields
VectorNTI Explorer
VectorNTI Explorer
VectorNTI Explorer
VectorNTI Explorer
VectorNTI Explorer
VectorNTI Explorer
VectorNTI Explorer Paste the data in e.g. Excel
VectorNTI Explorer ,[object Object],[object Object],[object Object],[object Object],[object Object]
VectorNTI Explorer ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
VectorNTI Explorer http://Rebase.neb.com/rebase/rebase.html
VectorNTI Explorer
VectorNTI Explorer Save file as... » bairoch.nnn R
VectorNTI Explorer ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object],MAIN MENU Links to the other modules of the Vector NTI suite
VectorNTI ,[object Object],MAIN TOOLBAR Changeable toolbar Depending on which pane is active
VectorNTI ,[object Object],TEXT WINDOW
VectorNTI ,[object Object],GRAPHICS WINDOW
VectorNTI ,[object Object],SEQUENCE WINDOW
VectorNTI ,[object Object],Local Database
VectorNTI ,[object Object],Open the local database
VectorNTI ,[object Object],Click pACYC177 to open
VectorNTI ,[object Object],Resize windows for a better view
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object],Copy complete graphics 1
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object],but COPY (Ctrl+C) & PASTE (Ctrl+V) also works...
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object],Open the local database
VectorNTI ,[object Object],Click pBR322 to open
VectorNTI ,[object Object]
VectorNTI ,[object Object],Molecule display setup
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object],How to edit your molecule
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object],Link Panes
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI ,[object Object]
VectorNTI: Molecule Editing ,[object Object],[object Object],[object Object]
VectorNTI: Molecule Editing ,[object Object],Retrieve directly from NCBI into VectorNTI: » Tools » Open » Retrieve... NM_001025366
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object],Ctrl + click » ARRANGEMENT
VectorNTI: Molecule Editing ,[object Object],Graphics Display Setup >> all features will be displayed in their specific way
VectorNTI: Molecule Editing ,[object Object],Feature Display Setup >> change the way this feature is displayed
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object],Picture Editing Mode
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object],[object Object],[object Object],[object Object],[object Object]
VectorNTI: Molecule Editing ,[object Object],Change graphical display
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Molecule Editing ,[object Object]
VectorNTI: Restriction enzymes ,[object Object]
VectorNTI: Restriction enzymes ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
VectorNTI: Restriction enzymes ,[object Object]
VectorNTI: Restriction enzymes ,[object Object]
VectorNTI: Restriction enzymes ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object],Select 2 enzymes (use Ctrl) e.g. PvuI and EcoRV
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object],Restriction Analysis and Report
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object],Select BamHI and PvuI
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
VectorNTI: Restriction enzymes ,[object Object]
VectorNTI: Restriction enzymes ,[object Object]
VectorNTI: Restriction enzymes ,[object Object]
VectorNTI: Restriction enzymes ,[object Object]
VectorNTI: Restriction enzymes ,[object Object]
VectorNTI: Restriction enzymes ,[object Object]
VectorNTI: Restriction enzymes ,[object Object]
Primer design and PCR analysis ,[object Object],[object Object],[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Primer design and PCR analysis ,[object Object]
Molecule construction (cloning) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Primer design and PCR analysis ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object],hold shift key and click in RE! else Fragment wizard reverts to 5’!
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object],hold shift key and click in RE! else Fragment wizard reverts to 5’!
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object]
Molecule construction (cloning) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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Vnti11 basics course

Editor's Notes

  1. Import Invitrogen molecules (C:\\VNTI Database\\DBINIT.MA4) from archive
  2. Search for all NCBI Entrez molecules
  3. Search for all NCBI Entrez molecules
  4. Add the search results in a new subset NCBI Entrez
  5. Move the subset to the public molecules
  6. Add your own fields