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Lecture 11 Recombinant Dna Tech

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1. Recombinant DNA technology involves manipulating DNA from different species and combining them to form new recombinant DNA molecules. 2. Key steps include using restriction enzymes to cut DNA at specific sites, and DNA ligase to join DNA fragments together into vectors like plasmids. 3. The recombinant DNA can then be replicated in host cells like bacteria to produce multiple copies for analysis.

Technology
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Recombinant DNA technology
Essential vocabulary ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Biotechnology ,[object Object],.
RDNA ,[object Object]
RDNA tech ,[object Object],[object Object],[object Object]
RDNA tech process ,[object Object],[object Object],[object Object],[object Object]
steps ,[object Object],[object Object],[object Object],[object Object],The cut segments are inserted into other DNA molecules that serves as vectors
Vectors ,[object Object],[object Object],[object Object],[object Object]
Plasmids ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Resctriction enzymes ,[object Object],[object Object],[object Object],[object Object],[object Object]
 
Specific palindromic sites
 
 
Library construction ,[object Object],[object Object],[object Object]
 
summary
[object Object],Used widely in research and hospital laboratories. Broad applications - medicine, agriculture,
??.. ,[object Object],[object Object],[object Object]
Applications ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Medicine ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],,
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Vaccines ,[object Object],[object Object],[object Object],[object Object]
Animals ,[object Object],[object Object]
Agriculture ,[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
Agriculture ,[object Object],[object Object],[object Object],[object Object]

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