The document discusses the different types of nucleic acids that viruses can use to store their genetic information, including double-stranded DNA, single-stranded DNA, double-stranded RNA, negative-sense RNA, positive-sense RNA, and positive-sense RNA retroviruses. It provides details on how each type replicates and produces viral mRNA.
SYNTHETIC PEPTIDE VACCINES AND RECOMBINANT ANTIGEN VACCINED.R. Chandravanshi
What is a Vaccine?
A vaccine is a substance that is introduced into the body to prevent infection or to control disease due to a certain pathogen (a disease-causing organism, such as a virus, bacteria or parasite). The vaccine “teaches” the body how to defend itself against the pathogen by creating an immune response.
1 Unlike traditional pharmaceuticals, vaccines are biologics since they are made from living organisms (biological sources).
2 Specifically, vaccines are preparations of components derived from (or related to) a pathogen; they can typically induce a protective effect through one to three very small doses, in the range of micrograms to milligrams.
3 Immunity lasts for an extended period, from one year up to lifetime protection, including prevention of disease and/or related sequelae.
Synthetic peptide vaccines represent fragments of protein antigen sequences, synthesizing specific B cell and T cell epitopes offer the potential to induce diseases neutralizing immuno response with completely synthetic structure. Now it is well established that short chain peptides can be used to mimic antigenic sites of viruses and thus can be used the basics for vaccines and development. therefore, attempts have been made to synthesize such peptides which act as the serrogate immuunogens, as an alternative to the existing conventional vaccines.
CLONAL SELECTION THEORY IS AN SCIENTIFIC THEORY IN IMMUNOLOGY THAT EXPALINS THE FUNCTION OF CELLS OF THE IMMUNE SYSTEM IN RESPONSE TO SPECIFIC ANTIGEN INVADING THE BODY.
Ques-7Viruses contain DNA (deoxyribonucleic acid) or RNA (ribonuc.pdfaquacare2008
Ques-7:
Viruses contain DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) as their genetic
material, and they reproduce in the host cells. Bacteriophage is a type of virus that can infect
bacteria and inject its genome into the host, the bacteria then produces dozens of viral lineages.
Retrovirus such as HIV (human immune deficiency virus) reproduces in a different way, as they
contain reverse transcriptase enzyme. In this, a complementary DNA is transcribed from the
RNA genome, and it is called as DNA provirus.
The rate of evolution in RNA-based viruses (microcosm), like HIV-1 has million times higher
mutation rate when compared to that of DNA-based organisms (bacteria humans etc). It can
rapidly changes its genome components under biological conditions to acquire adaptations for
survival (resistance against drugs, immune components) in the biological host cell medium
compared to the DNA based organisms in which lower rate of evolutionary adaptations were
observed. This property of high genomic mutation rate in HIV-1 retrovirus is due to the
following mechanisms.
Viral genome transcribe via reverse transcription finally proved HIV-1 mRNA (after splicing)
with Rev 350 nucleotide sequences. These Rev produced once the viral mRNA transferred to the
cytoplasm from the nucleus for translation. Virulence is the factor of causing extensive damage
to the host cell and infecting new host cell in which pathogen is going to undergo extensive rate
of reproduction & phase variation along with genome modification to defend host immune
system. This is the main basis of evolution and an example ahs described below.
Several mutated viral lineages are produced due to the nucleotide integration into the host cells
& change their genome finally code for the enzymatic proteins such as reverse transcriptase,
ribonuclease, and integrase in order to promote severe multiplication of integrated host genome
with viral protein predominanlty result in several lineages (multiple capies of virions).
For example: Viral evolution mechanism with new genome & producetion of several viral
lineages
1. After viral particle entry, viral genome integrates into the host cell genome and replicate
followed by expression. Later the novel synthesized viral proteins are going to be transported to
specific sites for assembly into progeny virus thereby-----> more assembly/progeny
2. Even though viral assembly is going to takes place in the host cell plasma membrane, but a
variety of viral genomes initiate assembly in intracellular organelles or nucleus predominantly.
Thereby further particle/plaque forming units associate with exocytosis of the viral and host cell
integrated genome after lysis. Finally again mature viral lineages are going to target new cells.
Reverse transcriptase induced complementary DNA synthesis followed by mRNA synthesis for
viral protein -coding repoitre. This enzyme enables to produce more viral genome particle by
integrating into the host cell followed by vir.
SYNTHETIC PEPTIDE VACCINES AND RECOMBINANT ANTIGEN VACCINED.R. Chandravanshi
What is a Vaccine?
A vaccine is a substance that is introduced into the body to prevent infection or to control disease due to a certain pathogen (a disease-causing organism, such as a virus, bacteria or parasite). The vaccine “teaches” the body how to defend itself against the pathogen by creating an immune response.
1 Unlike traditional pharmaceuticals, vaccines are biologics since they are made from living organisms (biological sources).
2 Specifically, vaccines are preparations of components derived from (or related to) a pathogen; they can typically induce a protective effect through one to three very small doses, in the range of micrograms to milligrams.
3 Immunity lasts for an extended period, from one year up to lifetime protection, including prevention of disease and/or related sequelae.
Synthetic peptide vaccines represent fragments of protein antigen sequences, synthesizing specific B cell and T cell epitopes offer the potential to induce diseases neutralizing immuno response with completely synthetic structure. Now it is well established that short chain peptides can be used to mimic antigenic sites of viruses and thus can be used the basics for vaccines and development. therefore, attempts have been made to synthesize such peptides which act as the serrogate immuunogens, as an alternative to the existing conventional vaccines.
CLONAL SELECTION THEORY IS AN SCIENTIFIC THEORY IN IMMUNOLOGY THAT EXPALINS THE FUNCTION OF CELLS OF THE IMMUNE SYSTEM IN RESPONSE TO SPECIFIC ANTIGEN INVADING THE BODY.
Ques-7Viruses contain DNA (deoxyribonucleic acid) or RNA (ribonuc.pdfaquacare2008
Ques-7:
Viruses contain DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) as their genetic
material, and they reproduce in the host cells. Bacteriophage is a type of virus that can infect
bacteria and inject its genome into the host, the bacteria then produces dozens of viral lineages.
Retrovirus such as HIV (human immune deficiency virus) reproduces in a different way, as they
contain reverse transcriptase enzyme. In this, a complementary DNA is transcribed from the
RNA genome, and it is called as DNA provirus.
The rate of evolution in RNA-based viruses (microcosm), like HIV-1 has million times higher
mutation rate when compared to that of DNA-based organisms (bacteria humans etc). It can
rapidly changes its genome components under biological conditions to acquire adaptations for
survival (resistance against drugs, immune components) in the biological host cell medium
compared to the DNA based organisms in which lower rate of evolutionary adaptations were
observed. This property of high genomic mutation rate in HIV-1 retrovirus is due to the
following mechanisms.
Viral genome transcribe via reverse transcription finally proved HIV-1 mRNA (after splicing)
with Rev 350 nucleotide sequences. These Rev produced once the viral mRNA transferred to the
cytoplasm from the nucleus for translation. Virulence is the factor of causing extensive damage
to the host cell and infecting new host cell in which pathogen is going to undergo extensive rate
of reproduction & phase variation along with genome modification to defend host immune
system. This is the main basis of evolution and an example ahs described below.
Several mutated viral lineages are produced due to the nucleotide integration into the host cells
& change their genome finally code for the enzymatic proteins such as reverse transcriptase,
ribonuclease, and integrase in order to promote severe multiplication of integrated host genome
with viral protein predominanlty result in several lineages (multiple capies of virions).
For example: Viral evolution mechanism with new genome & producetion of several viral
lineages
1. After viral particle entry, viral genome integrates into the host cell genome and replicate
followed by expression. Later the novel synthesized viral proteins are going to be transported to
specific sites for assembly into progeny virus thereby-----> more assembly/progeny
2. Even though viral assembly is going to takes place in the host cell plasma membrane, but a
variety of viral genomes initiate assembly in intracellular organelles or nucleus predominantly.
Thereby further particle/plaque forming units associate with exocytosis of the viral and host cell
integrated genome after lysis. Finally again mature viral lineages are going to target new cells.
Reverse transcriptase induced complementary DNA synthesis followed by mRNA synthesis for
viral protein -coding repoitre. This enzyme enables to produce more viral genome particle by
integrating into the host cell followed by vir.
Double branded DNA viruses (select only one answer) all insert their.pdfarchgeetsenterprises
Double branded DNA viruses (select only one answer) all insert their genome into the host\'s
genome to be translated and transcribed likely have a slower rates of mutation than RNA viruses
never cause diseases are more common than RNA viruses are responsible for 45% of the
human genome because of their insertions
Solution
The double-stranded DNA viruses are unique pathogens which require a DNA intermediate for
replication of new viral particles using reverse transcriptase enzyme. These viruses are
particularly shown to be highly active pathogens for human and higher vertebrate infections from
primitive times. They are less common than RNA viruses. However, like other RNA/DNA
viruses, they too possess high mutation rates owing to faulty replication and lack of proof-
reading by polymeraes. Importantly, as a mode of infection, all of these viruses do insert their
genome or a part of it in the host cell\'s genome to promotes its transcription and translation. This
is why double-stranded DNA viruses are difficult to eradicate from human/vertebrate hosts
because they very easily modulate their molecular functions according to the host machinery.
Some examples of diseases caused by such viruses are small pox, herpes infection etc.
Thus, the above explanations states that choice 1 is most correct..
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
Ethnobotany and Ethnopharmacology:
Ethnobotany in herbal drug evaluation,
Impact of Ethnobotany in traditional medicine,
New development in herbals,
Bio-prospecting tools for drug discovery,
Role of Ethnopharmacology in drug evaluation,
Reverse Pharmacology.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
How to Split Bills in the Odoo 17 POS ModuleCeline George
Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
The Art Pastor's Guide to Sabbath | Steve ThomasonSteve Thomason
What is the purpose of the Sabbath Law in the Torah. It is interesting to compare how the context of the law shifts from Exodus to Deuteronomy. Who gets to rest, and why?
Digital Tools and AI for Teaching Learning and Research
Viral molecular genetics
1. Viral Genetics
Viruses can store their genetic information in
six different types of nucleic acid
which are named based on how that nucleic
acid eventually becomes transcribed to the
viral mRNA
Only a (+) viral mRNA strand can be
translated into viral protein
2. Viral Genetics
(+/-) double-stranded DNA
DNA-dependent DNA polymerase enzymes copy both
the (+) and (-) DNA strands
DNA-dependent RNA polymerase enzymes copy the
(-) DNA strand into (+) viral mRNA
Examples include most bacteriophages,
Papovaviruses, Adenoviruses, and Herpesviruses
3. Replication of a Double-Stranded DNA Viral Genome and
production of Viral mRNA
4. Viral Genetics
(+) single-stranded DNA
DNA-dependent DNA polymerase enzymes copy the
(+) DNA strand of the genome producing a dsDNA
intermediate
DNA-dependent RNA polymerase enzymes copy the
(-) DNA strand into (+) viral mRNA
Phage M13 and Parvoviruses
5. Replication of a Single-Stranded DNA Viral Genome and
Production of Viral mRNA
6. Viral Genetics
(+/-) double-stranded RNA
RNA-dependent RNA polymerase enzymes copy both
the (+) RNA and (-) RNA strands of the genome
producing a dsRNA genomes
RNA-dependent RNA polymerase enzymes copy the
(-) RNA strand into (+) viral mRNA
Reoviruses
7. Replication of a Double-Stranded RNA Viral Genome
and Production of Viral mRNA
8. Viral Genetics
(-) RNA
RNA-dependent RNA polymerase enzymes then copy
the (+) RNA strands producing ss (-) RNA viral
genome
RNA-dependent RNA polymerase enzymes then copy
the (+) RNA strands producing ss (-) RNA viral
genome
Orthomyxoviruses, Paramyxoviruses, Rhabdoviruses
9. Replication of a Single-Stranded Minus RNA Viral
Genome and Production of Viral mRNA
10. Viral Genetics
(+) RNA
RNA-dependent RNA polymerase enzymes copy the
(+) RNA genome producing ss (-) RNA
RNA-dependent RNA polymerase enzymes then copy
the (-) RNA strands producing ss (+) RNA viral
genome
Picornaviruses, Togaviruses, and Coronaviruses
11. Replication of a Single-Stranded Plus RNA Viral Genome
and Production of Viral mRNA
12. Viral Genetics
(+) RNA Retroviruses
reverse transcriptase enzymes (RNA-dependent DNA
polymerases) copy the (+) RNA genome producing ss
(-) DNA strands
DNA-dependent DNA polymerase enzymes then copy
the (-) DNA strands to produce a dsDNA intermediate
DNA-dependent RNA polymerase enzymes then copy
the (-) DNA strands to produce ss (+) RNA genomes
DNA-dependent RNA polymerase enzymes copy the
(-) DNA strand into (+) viral mRNA
HIV-1, HIV-2, and HTLV-1
13. Replication of a Single-Stranded Plus RNA Viral Genome
and Production of Viral mRNA by way of Reverse
Transcriptase
14. Viral Genetics
Viruses grow rapidly, there are usually a large
number of progeny virions per cell. There is,
therefore, more chance of mutations occurring over a
short time period
Viruses undergo genetic change by several
mechanisms
Genetic drift: where individual bases in the DNA or
RNA mutate to other bases
Antigenic shift: where there is a major change in the
genome of the virus. This occurs as a result of
recombination
15. Mutants
Spontaneous mutations
These arise naturally during viral replication
(Replication, Tautomeric base pairing)
DNA viruses tend to more genetically stable than
RNA viruses (DNA repair)
Induced mutation by physical (UV light or X-rays) or
chemical means (nitrous acid)
16. Mutants
Types of mutation
point mutants
insertion/deletion mutants
17. Phenotypic changes seen in virus mutants
Conditional lethal mutants:These mutants multiply
under some conditions but not others
A.temperature sensitive
B.host range
18. Phenotypic changes seen in virus mutants
Plaque size :may be larger or smaller than in the wild
type virus
Drug resistance: The possibility of drug resistant
mutants arising must always be considered
Enzyme-deficient mutants: Some viral enzymes are
not always essential and so we can isolate viable
enzyme-deficient mutants
19. Phenotypic changes seen in virus mutants
"Hot" mutants
These grow better at elevated temperatures than the
wild type virus.
They may be more virulent since host fever may
have little effect on the mutants but may slow down
the replication of wild type virions
20. Phenotypic changes seen in virus mutants
Attenuated mutants
Many viral mutants cause much milder symptoms (or
no symptoms) compared to the parental virus - these
are said to be attenuated
vaccine development
21. Recombination
Exchange of genetic information between two
genomes
"Classic" recombination :This involves breaking of
covalent bonds within the nucleic acid, exchange of
genetic information, and reforming of covalent bonds
This kind of break/join recombination is common in
DNA viruses or those RNA viruses which have a DNA
phase (retroviruses). The host cell has recombination
systems for DNA
22. Recombination
Recombination of this type is very rare in RNA viruses
(No host enzymes)
"copy choice" kind of mechanism in which the
polymerase switches templates while copying the
RNA
So far, there is no evidence for recombination in the
negative stranded RNA viruses giving rise to viable
viruses
24. Reassortment
Reassortment is a non-classical kind of recombination
If a virus has a segmented genome and if two
variants of that virus infect a single cell, progeny
virions can result with some segments from one
parent, some from the other
This is an efficient process - but is limited to viruses
with segmented genomes
orthomyxoviruses, reoviruses, arenaviruses, bunya
viruses
26. Applied genetics
vaccine called Flumist for influenza virus
The vaccine is trivalent – it contains 3 strains of
influenza virus
cold adapted strains: grow well at 25 degrees C
,grow in the upper respiratory tract
temperature-sensitive and grow poorly in the warmer
lower respiratory tract
viruses are attenuated strains and much less
pathogenic than wild-type virus
27. Applied genetics
The vaccine technology uses reassortment to
generate reassortant viruses which have six gene
segments from the
attenuated,
cold-adapted virus
and the HA and NA coding segments from the virus
which is likely to be a problem in the up-coming
influenza season
29. Complementation
Interaction at a functional level NOT at the nucleic
acid level
two mutants with a ts (temperature-sensitive) lesion
in different genes
neither can grow at a high temperature
infect the same cell with both mutants, each mutant
can provide the missing function of the other and
therefore they can replicate
30. Multiplicity reactivation
If double stranded DNA viruses are
inactivated using ultraviolet irradiation,
we often see reactivation if we infect
cells with the inactivated virus at a very
high multiplicity of infection?
31. Defective viruses
Defective viruses lack the full complement of genes
necessary for a complete infectious cycle (many are
deletion mutants)
they need another virus to provide the missing
functions - this second virus is called a helper virus
32. Defective interfering particles
The replication of the helper virus may be less
effective than if the defective virus (particle) was not
there
This is because the defective particle is competing
with the helper for the functions that the helper
provides
This phenomenon is known as interference, and
defective particles which cause this phenomenon are
known as "defective interfering" (DI) particles
Not all defective viruses interfere, but many do
33. Phenotypic mixing
If two different viruses infect a cell, progeny viruses
may contain coat components derived from both
parents and so they will have coat properties of both
parents
IT INVOLVES NO ALTERATION IN GENETIC
MATERIAL
We can also get the situation where a coat is entirely
that of another virus