1) Two techniques, bioassay and radioimmunoassay, are used to measure levels of the antidiuretic hormone arginine vasopressin (AVP) but radioimmunoassay is more sensitive and specific. 2) Basal circulating levels of AVP are very low, below the detection limit of most assays, so urinary concentrations are studied instead as a proxy for plasma levels. Patterns of urinary excretion correlate with known stimuli and disorders of AVP secretion. 3) The two techniques generally show close correlation, though radioimmunoassay sometimes detects slightly higher levels, possibly due to non-specific interference rather than biologically inactive fragments.