Carbohydrates are generally classified into monosaccharides (simple sugars), oligosaccharides (containing few sugar units) and polysaccharides (containing many sugar units).
Monosaccharides are sugar molecules containing short chain of carbon atoms, one aldehydic or ketonic group and hydroxyl groups attached to remaining Carbon atoms.
Oligosaccharides are formed by polymerisation of monosaccharide molecules by elimination of water molecules.
Polysaccharides are high molecular weight substances composed of large number of moosaccharide units combined to form one large polymer molecule. They may be straight chain or branched chain polymers.
it is about how ammonia is detoxified to urea and its biomedical significance. This PPT can be used by students of MBBS, MD, BDS and general Biochemistry students
Formation and fate of Ammonia
Transdeamination, oxidative and non oxidative deamination, Ammonia transport, Ammonia intoxication, Ammonia detoxification
Urea Cycle | Energetics of Urea Cycle | Regulation of Urea Cycle | Metabolic ...kiransharma204
This PPT contains topics related to Urea Cycle, Energetics of Urea Cycle, Regulation of Urea Cycle, Metabolic disorder of Urea Cycle & Clinical Importance.
Books referred: https://www.amazon.in/Biochemistry-2019-Satyanarayana-Satyanarayana-Author/dp/B07WGHCTKZ/ref=sr_1_1?crid=3FLX88MWT4Y30&dchild=1&keywords=satyanarayan+biochemistry&qid=1591701828&s=books&sprefix=satyanarayan+%2Cstripbooks%2C387&sr=1-1
Carbohydrates are generally classified into monosaccharides (simple sugars), oligosaccharides (containing few sugar units) and polysaccharides (containing many sugar units).
Monosaccharides are sugar molecules containing short chain of carbon atoms, one aldehydic or ketonic group and hydroxyl groups attached to remaining Carbon atoms.
Oligosaccharides are formed by polymerisation of monosaccharide molecules by elimination of water molecules.
Polysaccharides are high molecular weight substances composed of large number of moosaccharide units combined to form one large polymer molecule. They may be straight chain or branched chain polymers.
it is about how ammonia is detoxified to urea and its biomedical significance. This PPT can be used by students of MBBS, MD, BDS and general Biochemistry students
Formation and fate of Ammonia
Transdeamination, oxidative and non oxidative deamination, Ammonia transport, Ammonia intoxication, Ammonia detoxification
Urea Cycle | Energetics of Urea Cycle | Regulation of Urea Cycle | Metabolic ...kiransharma204
This PPT contains topics related to Urea Cycle, Energetics of Urea Cycle, Regulation of Urea Cycle, Metabolic disorder of Urea Cycle & Clinical Importance.
Books referred: https://www.amazon.in/Biochemistry-2019-Satyanarayana-Satyanarayana-Author/dp/B07WGHCTKZ/ref=sr_1_1?crid=3FLX88MWT4Y30&dchild=1&keywords=satyanarayan+biochemistry&qid=1591701828&s=books&sprefix=satyanarayan+%2Cstripbooks%2C387&sr=1-1
The urea cycle, also known as the ornithine cycle, is a biochemical pathway that plays a crucial role in the removal of toxic ammonia from the body. It takes place primarily in the liver and involves a series of enzymatic reactions that convert ammonia into urea, a less toxic compound that can be excreted through urine.
In ureotelic organisms, the ammonia deposited in
the mitochondria of hepatocytes is converted to urea in
the urea cycle. This pathway was discovered in 1932 by
Hans Krebs (who later also discovered the citric acid cycle)
and a medical student associate, Kurt Henseleit.
Urea production occurs almost exclusively in the liver
and is the fate of most of the ammonia channeled there.
The urea passes into the bloodstream and thus to the
kidneys and is excreted into the urine. The production
of urea now becomes the focus of our discussion.
designed for undergraduate level teaching of nitrogen metabolism in biochemistry. this is first in the series of three lectures. ideal for MBBS level teaching
The urea cycle is the metabolic pathway that transforms nitrogen to urea for excretion from the body. Liver cells play a critical role in disposing of nitrogenous waste by forming urea hrough the action of the urea cycle.
Nitrogenous excretory products are then removed from the body through in the urine.
The urea excreted each day by a healthy adult (about 30 g) accounts for about 90% of the nitrogenous excretory products.
The cycle occurs mainly in the liver.
A detailed study of Transition Metal Complexes of a Schiff base with its Phys...Abhishek Ghara
The many activities of metal ions in biology have stimulated the development of metal based therapeutics. It has been found that biologically active compounds become more effective and bacteriostatic upon chelation with metal ions also the biological activity of many drugs has been shown to be enhanced on complexing with metal ions, hence promoting their use in Pharmacology. The present work deals with the synthesis of metal complexes derived from a novel Schiff base drug synthesized from urea and salicylaldehyde and its physico-chemical analysis to find out ligand- metal ratio of this complex in solution. For the structure elucidation of these complexes “Monovariation method (Mole ratio method/ Yoe-Jones Method)” has been used to ascertain the ligand-metal ratio in the complex. The stability constant of the formed complex was calculated by molar conductance measurement using Modified Job’s method (Method of Continuous Variations). The analysis has been carried out using conductometry. To confirm metal-ligand ratio, conductometric titrations were carried out at room temperature using analytical grade metal salts. Titrations were carried out with “systronics conductivity-meter” using dip type conductivity cell having cell constant 1 at room temperature.
1. NETAJI SUBHAS CHANDRA BOSE INSTITUTE OF PHARMACY
Tatla, Roypara, Chakdaha, Dist. Nadia, PIN 741222, W.B.
Affiliated to
MAULANA ABUL KALAM AZAD UNIVERSITY OF TECHNOLOGY (MAKAUT)
BF 142, Sector 1, Salt Lake City, Kolkata 700064, West Bengal
2. INTRODUCTION:
Urea cycle is the first metabolic cycle to be elucidated.
The cycle is known as Krebs-Henseleit Urea cycle.
Ornithine is the first member of the reaction, it is also called as Ornithine cycle.
Urea is the major end product of protein metabolism (amino acid metabolism) in
humans and mammals.
Urea has two amino (-NH2) groups, one derived from NH3 and the other from
aspartate.
Urea is synthesized in the liver.
Than secreted into blood stream.
And taken up by the kidneys for excretion in the urine.
Urea synthesis is a five step cyclic process, with five distinct enzymes.
The first two enzymes are present in mitochondria while the rest are localized in
cytosol.
3. CHARACTERISTICS:
Urea is the major disposal form of amino groups.
It accounts for 90% of the nitrogen containing components of urine.
The urea cycle is the sole source of endogenous production of arginine.
Urea formation takes place in liver.
Urea excretion occurs through kidney.
4. SYNTHESIS:
STEP I: - Formation of carbamoyl phosphate
CO2 + NH4 + 2 ATP Carbamoyl Phosphate + 2
ADP + 2Pi
Carbamoyl Phosphate Synthase-I
N-Acetyl Glutamic acid
STEP II: - Formation of citrulline
Ornithine + Carbamoyl Phosphate Citrulline + Pi
Ornithine Transcarbamoylase
STEP III: - Formation of Arginosuccinate
Citrulline + Aspartate + ATP
Arginosuccinate synthase
Arginosuccinate +
AMP + PPi
5. STEP IV: - Formation of Arginine
STEP V: - Formation of Urea
Arginosuccinate Arginine + Fumarate
Arginosuccinase
Arginine + H2O
Arginase
Urea + Ornithine
This Ornithine again bind with Carbamoyl Phosphate to form Citrulline. That’s why it is a cyclic
process.
6. Overall reaction and energetic:
The urea cycle is irreversible and consumes 4 ATP. Two ATP are utilized for the synthesis of
carbamoyl phosphate. One ATP is converted to AMP and PPi to produce arginosuccinate which
equals to 2 ATP. Hence 4 ATP are actually consumed.
7. Regulation of Urea Cycle: -
Carbamoyl phosphate synthase (CPS-I) is rate limiting enzyme in Urea cycle.
CPS–I is allosterically activated by N–acetylglutamate (NAG).
It is synthesized from glutamate and acetyl CoA by synthase and degraded by a hyrolase.
The rate of Urea synthesis in liver is correlated with the concentration of N–acetylglutamate.
Formation and degradation of N-acetylglutamate
10. Blood Urea Significance: -
Normal blood Urea concentration is 10-40 mg/dl.
About 15-30 gm of Urea (7-15 gm nitrogen) is excreted in Urine per day.
Blood Urea estimation is a screening test for the evaluation of kidney(renal) function.
Elevation in blood Urea may be broadly classified into three categories.
11. Blood Urea Significance (continued): -
Renal:
In renal disorders like acute glomerulonephritis, chronic nephritis, nephrosclerosis, polycystic
kidey, blood Urea is increased.
Post-renal:
Due to obstruction in the Urinary tract (e.g. tumors, stones, enlargement of prostate gland
etc.) blood Urea is elevated.
This is due to increased reabsorption of Urea from the tubules.
Pre-renal:
This is associated with increased protein breakdown, leading to a negative nitrogen balance.
Observed after major surgery, prolonged fever, diabetic coma, thyrotoxicosis etc.
In leukemia & bleeding disorders also, blood Urea is elevated.
12. REFERENCES
1. Book of Biochemistry, by U. Satyanarayana & U. Chakrapani, Third
Edition, Page no. 337-341.
2. www.slideshare.net
3. www.fppt.com