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KEY CONCEPT DNA fingerprints identify people at the molecular level.
A DNA fingerprint is a type of restriction map. DNA fingerprints are based on parts of an individual’s DNA that can by used for identification. based on noncoding regions of DNA noncoding regions have repeating DNA sequences number of repeats differs between people Gel electrophoresis separates the sequences into “bands”
(mother) (child 1) (child 2) (father) DNA fingerprinting is used for identification.  DNA fingerprinting depends on the probability of a match. Many people have thesame number ofrepeats in a certainregion of DNA. The probability that two people share identicalnumbers of repeats inseveral locations isvery small.
[object Object]
evidence in criminal cases
paternity tests
immigration requests
studying biodiversity
 tracking genetically   modified crops,[object Object]
Entire organisms can be cloned. A clone is a genetically identical copy of a gene or of an organism.  If you could clone your favorite pet, would you?  Why or why not?
[object Object]
bacteria (binary fission)
some plants (from roots)
some simple animals (budding, regeneration),[object Object]
nucleus is removed from a fertilized egg cell
nucleus of a somatic cell from the animal to be cloned is implanted in the egg,[object Object]
organs for transplant into humans
save endangered species
Cloning raises concerns.  Like what?
low success rate
clones “imperfect” and less healthy than original animal
decreased biodiversity,[object Object]
(bacterial DNA) New genes can be added to an organism’s DNA.  Genetic engineering involves changing an organism’s DNA to give it new traits.  What genes would you like to incorporate into your favorite pet? Genetic engineering is based on the use of recombinant DNA.  Recombinant DNA contains genes from more than one organism.

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Unit 18a DNA fingerprinting and genetic engineering

  • 1. KEY CONCEPT DNA fingerprints identify people at the molecular level.
  • 2. A DNA fingerprint is a type of restriction map. DNA fingerprints are based on parts of an individual’s DNA that can by used for identification. based on noncoding regions of DNA noncoding regions have repeating DNA sequences number of repeats differs between people Gel electrophoresis separates the sequences into “bands”
  • 3. (mother) (child 1) (child 2) (father) DNA fingerprinting is used for identification. DNA fingerprinting depends on the probability of a match. Many people have thesame number ofrepeats in a certainregion of DNA. The probability that two people share identicalnumbers of repeats inseveral locations isvery small.
  • 4.
  • 9.
  • 10. Entire organisms can be cloned. A clone is a genetically identical copy of a gene or of an organism. If you could clone your favorite pet, would you? Why or why not?
  • 11.
  • 14.
  • 15. nucleus is removed from a fertilized egg cell
  • 16.
  • 17. organs for transplant into humans
  • 21. clones “imperfect” and less healthy than original animal
  • 22.
  • 23. (bacterial DNA) New genes can be added to an organism’s DNA. Genetic engineering involves changing an organism’s DNA to give it new traits. What genes would you like to incorporate into your favorite pet? Genetic engineering is based on the use of recombinant DNA. Recombinant DNA contains genes from more than one organism.
  • 24.
  • 25. plasmids are loops of DNA in bacteria
  • 26. restriction enzymes cut plasmid and foreign DNA
  • 27.
  • 28.
  • 32. Transgenic plants are common in agriculture.
  • 35.
  • 36. transgenic mice used to study development and disease
  • 37.
  • 38. possible long-term health effects of eating GM foods
  • 39.
  • 40. True or False? Sometimes, scientific knowledge leads to ethical dilemmas. Correct Answer = True A deontologist would likely believe lying is always wrong. Correct Answer =
  • 41. True or False? Sometimes, scientific knowledge leads to ethical dilemmas. Correct Answer = True A deontologist would likely believe lying is always wrong. Correct Answer = True In an ethical debate, if consensus is reached, the majority win as in a vote. Correct Answer =
  • 42. True or False? Sometimes, scientific knowledge leads to ethical dilemmas. Correct Answer = True A deontologist would likely believe lying is always wrong. Correct Answer = True In an ethical debate, if consensus is reached, the majority win as in a vote. Correct Answer = False Bias is not an issue on the World Wide Web. Correct Answer =
  • 43. True or False? Sometimes, scientific knowledge leads to ethical dilemmas. Correct Answer = True A deontologist would likely believe lying is always wrong. Correct Answer = True In an ethical debate, if consensus is reached, the majority win as in a vote. Correct Answer = False Bias is not an issue on the World Wide Web. Correct Answer = False A consequentialist would be likely to use a cost-benefit analysis. Correct Answer =
  • 44. True or False? Sometimes, scientific knowledge leads to ethical dilemmas. Correct Answer = True A deontologist would likely believe lying is always wrong. Correct Answer = True In an ethical debate, if consensus is reached, the majority win as in a vote. Correct Answer = False Bias is not an issue on the World Wide Web. Correct Answer = False A consequentialist would be likely to use a cost-benefit analysis. Correct Answer = True
  • 45. Review DNA fingerprinting is used for identification and determining relationship A clone is a genetically identical copy of a gene or of an organism – cloning occurs in nature Cloning has potential benefits and raises concerns Genetic engineering involves changing an organism’s DNA to give it new traits. A transgenic organism has one or more genes from another organism inserted into its genome Transgenic organisms are already being used both in agriculture and scientific studies Transgenics has potential benefits and raises concerns