1. DNA technology uses restriction enzymes to cut DNA into segments that can be spliced together to clone genes. Restriction enzymes recognize specific DNA sequences and cut the DNA. 2. Cloning vectors like plasmids are used to transfer genes between organisms. A donor gene is inserted into the plasmid which is then placed into a host cell to replicate the gene. 3. Recombinant DNA combines DNA from different sources, like inserting a human gene for insulin into bacterial DNA. The bacteria then produces insulin protein in large quantities to treat diabetes.