The document discusses key concepts in bioenergetics including:
1) Bioenergetics concerns the energy involved in making and breaking chemical bonds in molecules, which is fundamental to biological processes like growth that depend on energy transformations.
2) The first law of thermodynamics states that energy is conserved, while the second law states that entropy increases, reducing the available free energy.
3) Free energy (G) expresses the energy available to do work and depends on enthalpy (H) and entropy (S) changes. Exergonic reactions release free energy while endergonic reactions absorb it.
Formation and utilization of ketone bodies; ketoacidosisJinal Tandel
Formation and utilization of ketone bodies is part of lipid metabolism. After completion of this topic one can understand about Ketogenesis, utilization of Ketone bodies and ketoacidosis
Formation and utilization of ketone bodies; ketoacidosisJinal Tandel
Formation and utilization of ketone bodies is part of lipid metabolism. After completion of this topic one can understand about Ketogenesis, utilization of Ketone bodies and ketoacidosis
preparation of buffers, buffers and isotonic systems. Methods for
adjustment of tonicity of solutions. Buffers in pharmaceutical and biological systems.
This presentation was prepared in order to take Lecture of students in a summarised way and to provide them with the short, sweet and concise notes. It is based on PCI syllabus and is meant for B. Pharm. Second Semester...
preparation of buffers, buffers and isotonic systems. Methods for
adjustment of tonicity of solutions. Buffers in pharmaceutical and biological systems.
This presentation was prepared in order to take Lecture of students in a summarised way and to provide them with the short, sweet and concise notes. It is based on PCI syllabus and is meant for B. Pharm. Second Semester...
Reference Harper Illustrated book of Biochemistry
Applying laws of Thermodynamics to Biochemistry.
Diferent types of Reactions, exergonic and edergonic,
Illustrated explain of Role of ATP in our body,
Brief concept on ATP production and high energy phosphate,
ATP/ADP cycle and about Creatine Kinase
KEY CONCEPTS
8.1 An organism’s metabolism transforms matter and
energy, subject to the laws of thermodynamics
8.2 The free-energy change of a reaction tells us whether or not the reaction occurs
spontaneously
8.3 ATP powers cellular work by coupling exergonic reactions to endergonic reactions
8.4 Enzymes speed up metabolic reactions by lowering energy barriers
8.5 Regulation of enzyme activity helps control metabolism
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
2. Concept of free energy,
endergonic and exergonic reaction, Relationship between free energy,
enthalpy and entropy;
Redox potential.
Energy rich compounds;
classification;
biological significances of
ATP and cyclic AMP
Contents
3. Bioenergetics
Is the partof biochemistry concerned with the energy
involved in making and breaking of chemical bonds in
the molecules
The role of energy is fundamental to such biological
processes
Life isdependent on energy transformations;living
organisms survive because of exchange of energy
within and without.
Itisthe quantitative studyof the energy transductions
that occurin living cellsand of the nature and
function of the chemical processesunderlying these
transductions
4. Biochemical thermodynamics
In a living organism,chemicalbondsare broken and made
as part of the exchange and transformation of energy.
Energyisavailable forwork (such as mechanical work)orfor
other processes (such as chemical synthesisand anabolic
processes in growth),
when weak bonds are broken and stronger bonds are
made. Theproduction of strongerbondsallows release of
usable energy.
5. The FirstLaw of Thermodynamics, also know as the law
of conservation of energy,
It states that energy can neither be created nor
destroyed. It may change from one form to another,
but the energy in a closed system remains constant.
Inany physical or chemical change, the total amount
of energy in the universe remains constant,although
the form of energy may change.
6. The Second Law of Thermodynamics states that when
energy is transferred, law of entropy, there will be less
energy available at the end of the transfer process
than at the beginning.
Due to entropy, which isthe measure of disorder in a
closed system, all of the available energy will not be
useful to the organism.
Entropy increases as energy istransferred.
In all natural processes, the entropy of the universe
increases.
7. The amount of energy that is available to do work is
described by the concept of free energy
Gibbs free energy (G) expresses the amount of energy
capable of doing work during a reaction at constant
temperature and pressure
Exergonic reaction= A reaction that proceeds with a net
release of free energy and is spontaneous.
When a reaction proceeds with the release of free energy
(i.e., when the system changes so as to possess less free
energy),
Free-energy change, ΔG, has a negative sign and the
reaction is said to be exergonic.
•Endergonic reaction= An energy-requiring reaction that
proceeds with a net gain of free energy; a reaction that
absorbs free energy from its surroundings and non
spontaneous.
•In endergonic reactions, the system gains free energy and
ΔG is positive
8. Gibbs change in free energy (ΔG )is that portion of the total
energy change in a system available for doing work.
It is also known as the chemical potential .
ΔG= ΔH - TΔS.
Useful energy = change in Enthalpy – change in entropy
[∆G = Gibbs change in Free Energy; ∆H = Change in
Enthalpy; T = Temperature in K; ∆S = Change in Entropy]
Enthalpy, H, is the heat content of the reacting system.
It reflects the number and kinds of chemical bonds in the
reactants and products
The units of ΔG and ΔH are joules/mole or calories/mole
(recall that 1 cal equals 4.18 J);
Entropy, S, is a quantitative expression for the randomness or
disorder in a system.
units of entropy are joules/mole•degree Kelvin (J/mol•K)
9. The exergonic reaction is a type of
reaction in which free energy is released
Endergonic reactions are the type of
reaction in which free energy is absorbed.
Here Gibbs free energy is negative Here Gibbs free energy is positive
Exergonic reactions indicate that the
energy is
released in the system
Endergonic reactions indicate that the
energy is
absorbed by the system
All the exothermic reactions are
exergonic.
All endothermic reactions are
endothermic
Exergonic reactions do not require energy
to
begin
Endothermic reactions always require
energy to
begin
It is a downhill reaction It is an uphill reaction
Fatty Acid Catabolism, Glycolysis, cellular
respiration
DNA/RNA Synthesis, Protein synthesis,
Fatty acid
Synthesis
Exergonic reaction Endergonic reaction
10. (a) Exergonic reaction: energy released (b) Endergonic reaction: energy required
•If a chemical process is exergonic, the reverse process must be
endergonic.
•In cellular metabolism, endergonic reactions are driven by coupling
them to reactions with exergonic reactions.
•ATP plays a critical role in this energy coupling.
11. It is the affinity of a substance to accept electrons i.e. it
is the potential for a substance to become reduced.
Hydrogen has the lowest redox potential (-0.42 volt),
while oxygen has the highest redox potential (+0.82
volt).
The redox potentials of all other substances lie between
that of hydrogen and oxygen.
Electrons are transferred from substances with low
redox potential to substances with higher redox
potential.
This transfer of electrons is an energy yielding process
and the amount of energy liberated depends on the
redox potential difference between the electron donor
and acceptor.
Redox potential
12. The redox potential is a measure (in volts) of the
affinity of a substance for electrons — its
electronegativity — compared with hydrogen
13. Energy-rich compounds in cells comprise five
kinds of high-energy bonds:
phosphoanhydride, acyl phosphate,
enolphosphate, guanidine phosphate and
thioester bonds
The compounds are represented by adenosine
triphosphate (ATP).
substances capable of ATP formation in enzyme
reactions involving transfer of phosphate groups
Bonds in energy-rich compounds, which yields
high energy upon hydrolysis are called high-
energy bonds
14. ADENOSINE TRIPHOSPHATE (ATP)
✓ Adenosine-5'-triphosphate (ATP) is a
multifunctional nucleotide used in cells as a coenzyme.
✓ It is often called the "molecular unit of currency" of
intracellular energy transfer. ATP transports chemical energy
within cells for metabolism.
✓ It is produced by photophosphorylation and cellular
respiration and used by enzymes and structural proteins in
many cellular processes, including biosynthetic
reactions, motility, and cell division.
✓One molecule of ATP contains three phosphate groups and
it is produced by ATP synthase from inorganic
phosphate and adenosine diphosphate (ADP) or adenosine
monophosphate (AMP).
15. The structure of this molecule
consists of a purine base
(adenine) attached to the 1'
carbon atom of a pentose sugar
(ribose). Three phosphate groups
are attached at the 5' carbon
atom of the pentose sugar. It is
the addition and
removal of these phosphate
groups that inter-convert ATP,
ADP and AMP. When ATP is
used in DNA synthesis,
the ribose sugar is first converted
to deoxyribose by ribonucleotide
reductase
16. •ATP is an unstable molecule which hydrolyzes to ADP
and inorganic phosphate when it is in equilibrium with
water.
•The high energy of this molecule comes from the two
high-energy phosphate bonds.
•The bonds between phosphate molecules are called
phosphoanhydride bonds
ATP is hydrolyzed to ADP in reaction
ATP+H2O→ADP+Pi+ free energy;
calculated ∆G for 1 mole of ATP is -57 kJ/mol.
The hydrolysis of ATP produces 7 kcal/mole
(note: Calories is the same as kcal).
ADP is combined with a phosphate to form ATP in the
reaction ADP+Pi+free energy→ATP+H2O.
17. CYCLIC ADENOSINE MONOPHOSPHATE
(cAMP, cyclic AMP or 3'-5'-cyclic adenosine
monophosphate)
✓ It is a second messenger important in many biological
processes. cAMP is derived from adenosine triphosphate
(ATP) and used for intracellular signal transduction in
many different organisms, conveying the cAMP-
dependent
pathway.
✓ cAMP is synthesised from ATP by adenylyl
cyclase located on the inner side of the plasma
membrane.
18. Adenylyl cyclase is activated by a range of
signaling molecules through the activation
of adenylyl cyclase stimulatory G
(Gs)-protein-coupled receptors and inhibited
by agonists of adenylyl cyclase inhibitory G
(Gi)-protein-coupled
receptors. Liver adenylyl cyclase responds
more strongly to glucagon, and muscle
adenylyl cyclase responds more
strongly to adrenaline.
✓ cAMP decomposition into AMP is catalyzed
by the enzyme phosphodiesterase.
19. Function:
cAMP is a second messenger, used for
intracellular signal transduction,
such as transferring the effects
of hormones like glucagon and adrenaline,
which cannot pass through the cell membrane. It
is involved in the activation of protein kinases and
regulates the effects of adrenaline and glucagon.
It also regulates the passage of
Ca2+ through ion channels. cAMP and its
associated kinases function in several biochemical
processes, including
the regulation of glycogen, sugar, and lipid
metabolism by activating protein kinase
20. 🞇 The ADP/ATP Cycle
🞇 The ADP/ATP cycle
isa method for
renewing the supply
of ATP that is
constantly being
used up in the cell.
🞇 Energy input
couples inorganic
phosphate to ADP
to form energized
ATP
.
21. •References
•Lehninger-Principles of biochemisty (forth edition)
Authors-D.Nelson, M. Cox
•Biochemistry (fifth edition) Authors-J.berg, J.Tymoczko,
L.Styer
•Harper’s Illustrated Biochemistry(26th edition)
Authors-R.Murray, D.Granner, V.Rodwell
•Biochemistry(second edition) Authors-Garrett and Grisham
•Molecular biology of the cell Author-Bruce Albert
•Molecular cell biology(fifth edition) Authors-Lodish, Berk
• Bioenergetics, Lee, Thonylet E. Mabinta, Dianne Melad, Maria
Fe Power point presentation
• https://www.nicholls.edu/biol-
ds/biol155/Lectures/Biological%20Molecules.pdf