Stem cells can differentiate into various cell types and can self-renew. Mesenchymal stem cells are preferred for clinical applications as they do not raise ethical issues, avoid rejection, and have immunomodulatory properties. Mesenchymal stem cells from sources like umbilical cord, placenta, and dental pulp can differentiate into insulin-producing cells and help treat diabetes and its complications like cardiomyopathy, nephropathy, and wounds. While mesenchymal stem cells show promise, limitations include poor engraftment and differentiation in vivo.
What are stem cells? This presentation provides an overview of multiple different stem cells including embryonic stem cells, mesenchymal stem cells, cancer stem cells, induced pluripotent stem cells, hematopoietic stem cells and neural stem cells.
What are stem cells? This presentation provides an overview of multiple different stem cells including embryonic stem cells, mesenchymal stem cells, cancer stem cells, induced pluripotent stem cells, hematopoietic stem cells and neural stem cells.
Stem Cell Technology and its Clinical ApplicationDr. Barkha Gupta
Dr. Barkha Gupta has been teaching Veterinary Biochemistry as well as clinical physiology at CVAS, Udaipur and PGIVER, Jaipur. She has earlier served in various capacities in the Department of Animal Husbandry, Govt. of Rajasthan. She has several publications and awards to her credit. She is the PI of M-RAJUVAS Android Educational Mobile Application for Veterinary and Animal Sciences and Kiosk Information System for Farmers/Livestock Owners. Dr. Gupta is also IFBA Certified Professional.
Imagine that you have been told you have an illness that cannot be cured or what if your body has been irreversibly paralysed. There is no hope. But there is a science that could change that. It’s Called Stem Cell Research and it’s an important step in the medical revolution. But it comes with controversies as it uses Human Embryos’ as Raw Material.
But something astounding happened in the year 2006 that removed the usage of surplus embryos from the equation altogether. It’s about a brand new technology that can turn back the clock on your body cells. This is cutting edge of science where new developments are happing all the time. The iPSCs could be the potential medicine of 21st century. So what are stem cells? Why do they Matter? What are iPSCs and how it changed the biological rules?
Pluripotent Stem Cells and their applications in disease modelling, drug disc...tara singh rawat
This ppt gives an insight of the potential and possibilities of pluripotent stem cells research in disease modelling, drug discovery and regenerative medicine
This slide is all about the hematopoeitic stem cells its two types myeloid and lymphoid. The different types of myleoid and lymphoid cells are explained in details. All details about different White Blood Cells and their function. B cell, T cell and Natural Killer cell and their function.
Stem Cell Therapy Clinical Trial at Patients MedicalPatients Medical
Dr. Kamau Kokayi from the New York Stem Cell Treatment Center at Patients Medical gives the latest information on the amazing discoveries and healing capacity of stem cells and details on enrolling in the current clinical trial at NYSCTC.
A stem cell is a "blank" cell that can give rise to multiple tissue types such as a skin, muscle, or nerve cell.
Under certain physiologic or experimental conditions, they can be induced to become tissue- or organ-specific cells with special functions.
Stem Cell Technology and its Clinical ApplicationDr. Barkha Gupta
Dr. Barkha Gupta has been teaching Veterinary Biochemistry as well as clinical physiology at CVAS, Udaipur and PGIVER, Jaipur. She has earlier served in various capacities in the Department of Animal Husbandry, Govt. of Rajasthan. She has several publications and awards to her credit. She is the PI of M-RAJUVAS Android Educational Mobile Application for Veterinary and Animal Sciences and Kiosk Information System for Farmers/Livestock Owners. Dr. Gupta is also IFBA Certified Professional.
Imagine that you have been told you have an illness that cannot be cured or what if your body has been irreversibly paralysed. There is no hope. But there is a science that could change that. It’s Called Stem Cell Research and it’s an important step in the medical revolution. But it comes with controversies as it uses Human Embryos’ as Raw Material.
But something astounding happened in the year 2006 that removed the usage of surplus embryos from the equation altogether. It’s about a brand new technology that can turn back the clock on your body cells. This is cutting edge of science where new developments are happing all the time. The iPSCs could be the potential medicine of 21st century. So what are stem cells? Why do they Matter? What are iPSCs and how it changed the biological rules?
Pluripotent Stem Cells and their applications in disease modelling, drug disc...tara singh rawat
This ppt gives an insight of the potential and possibilities of pluripotent stem cells research in disease modelling, drug discovery and regenerative medicine
This slide is all about the hematopoeitic stem cells its two types myeloid and lymphoid. The different types of myleoid and lymphoid cells are explained in details. All details about different White Blood Cells and their function. B cell, T cell and Natural Killer cell and their function.
Stem Cell Therapy Clinical Trial at Patients MedicalPatients Medical
Dr. Kamau Kokayi from the New York Stem Cell Treatment Center at Patients Medical gives the latest information on the amazing discoveries and healing capacity of stem cells and details on enrolling in the current clinical trial at NYSCTC.
A stem cell is a "blank" cell that can give rise to multiple tissue types such as a skin, muscle, or nerve cell.
Under certain physiologic or experimental conditions, they can be induced to become tissue- or organ-specific cells with special functions.
stem cell research is yet to be advanced , once fully developed can alleviate human suffering, this ppt reviews the contemporary evidence, pitfalls and challenges
history ,definition,type of stem cells , characters of stem cells , source, stem cell banking , indications of stem cell therapy ,applications in gynaecology
Dr. Kenneth Dickie from Royal Centre of Plastic Surgery in Barrie, Ontario explained the use of stem cells technology in plastic surgery.
If you have any questions, please contact Dr. Kenneth Dickie at http://royalcentreofplasticsurgery.com/
What to know before getting stem cellsMegan Hughes
Dr. Hughes discusses what you should know before getting stem cells, which stem cells are best for specific problems, and what to expect after the procedure.
Stem cell Therapy in Neurological diseases Ibad khan
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difinition
mechanism
types
history
advantages or disadvantages
in this presentation all theses information include ,
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A review of the growth of the Israel Genealogy Research Association Database Collection for the last 12 months. Our collection is now passed the 3 million mark and still growing. See which archives have contributed the most. See the different types of records we have, and which years have had records added. You can also see what we have for the future.
A workshop hosted by the South African Journal of Science aimed at postgraduate students and early career researchers with little or no experience in writing and publishing journal articles.
This slide is special for master students (MIBS & MIFB) in UUM. Also useful for readers who are interested in the topic of contemporary Islamic banking.
Exploiting Artificial Intelligence for Empowering Researchers and Faculty, In...Dr. Vinod Kumar Kanvaria
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Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
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It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
2. Stem Cell
Stem Cells
• Are undifferentiated “master” cell that do not
have a specific function
Can change to one or several different cell
types (differentiate) under proper conditions
Can undergo unlimited cell division, self-
renewal.
5. Adult stem cell
• A subset of cells which maintain and repair
the tissues in which they reside.
• They are the attractive candidates for cell
based therapies as:
(i) They do not raise ethical conflicts.
(ii) They offer the scope of auto
transplantation without the problem of
allogenic graft rejection.
9. Immunological barriers
• Rejection- mediated by class 1 MHC and by
antigen presenting cells harbouring the class
2 MHC antigen.
• Can be overcome by marrow transplantation
to induce immunological control.
• Somatic Cell Nuclear Transfer.
10. MESENCHYMAL STEM CELLS
MESENCHYMAL STEM CELLS
ARE MOST PREFERED STEM
CELLS FOR CLINICAL
APPLICATION
11. No risk of No risk of
rejection – used teratoma Pluripotent
across HLA formation
barrier
Precise
No ethical identificatio
issues n
Benefits of Ease of
Immune mesenchymal isolation
and
privileged
stem cell scale up
Greater Genetic
potency of stability
cultured
expanded Lower cost Homogeneous
product Efficient large
of population and
scale
cell culture high rate of cell
expansion
process division
23. Characterization of human umbilical
cord mesenchymal stem cells
KI67 / Nuclei SMA / Nuclei Nestin Nuclei DesminNuclei Vimentin
Nuclei
Majority of the cells obtained from the cord showed homogenous mesenchymal
population (stained positive for CD44, 73, 90 and 117). The cells expressed the
mesenchymal markers such as nestin, vimentin, desmin and smooth muscle
actin. Oct4, an embryonic stem cells marker was also found to be expressed in
these cord derived MSCs.
24. Islet neogenesis from cord MSCs
Day 10 DTZ positive ILC
Day 0 Day 2
Nucleus/Glucagon/Insulin
Cord MSCs migrate to form Islet like Clusters (ILCs) upon exposure to Serum Free
Medium (SFM) supplemented with growth factors involved in pancreatic
development.
The ILCs are positive for DTZ staining and exhibit immunopositivity for pancreatic
hormones viz; insulin and glucagon.
37. Diabetic Nephropathy
• Injected MSCs engraft in damaged kidneys,
differentiate into renal cells, and regulate the
immune response resulting in an efficient treatment
of diabetic nephropathy .
• Additionally, the small percentage of hMSCs in the
transplanted kidneys differentiated into endothelial
cells as evidenced by de novo expression of CD31
• MSCs are able to reconstitute necrotic segments of
diabetic kidneys
38. Wound Healing
• Systemic (I.V) and local administration of bone
marrow-derived MSCs significantly
increased collagen levels followed by increased
wound-breaking strength I
moderate (TGF-β, KGF) or significant (EGF, PDGF,
and VEGF) increase.
neovascularization and formation of inflammation
infiltrate, containing predominantly mononuclear
cells, without tissue necrosis
formed sweat or sebaceous gland-like structures of
the skin.
39. Diabetic foot
• Infusions of stem cells intra-arterially and I.M
in each foot possibly avoids the need for
amputation.
• Stem cell injection supposedly results in 70-80
percent improvement in pus and wounds
40.
41. Limitations of MSCs
• Poor engraftment and limited differentiation
under in vivo conditions
• The frequency of spontaneous differentiation
of MSCs in the host tissue is extremely rare
• Additional limitation is the potential of MSCs
to differentiate into unwanted mesenchymal
lineages
• Possible malignant transformation and
cytogenetic aberrations of MSCs.
42. Conclusion
• Because of their immunomodulatory ability,
self-renewal, and differentiation capacity,
MSCs are expected to become promising
therapeutic agents for improvement of
diabetes, it’s complications like DCM,
nephropathy, DPN, and wounds in diabetic
patients.