This document discusses plant tissue culture techniques. It describes different types of plant cell, tissue and organ cultures including micropropagation. It outlines the basic steps of tissue culture which are 1) initiation/explant preparation, 2) multiplication, 3) rooting and 4) acclimatization. Common growth media used include Murashige and Skoog medium and Woody Plant Medium. Equipment used in tissue culture includes laminar flow cabinets, autoclaves, centrifuges and incubators.

2. Plant Tissue Culture

3. Plant Tissue Culture
Plant Cell Culture(
Plant Tissue Culture(
Plant Organ Culture( (
Micropropagation

4. 1
2
3
4
5
6
7
8PH
9

5. -
-
Murashige & Skoog(MS
Woody Plant Medium(WPM(

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Micropipette
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7. autoclave-
- .
-
-
-
-
Ph

8. Laminar flow air 1
2
3
1,541
55,5
Tween20
6
Centrifuge 7
Protoplast isolation
8

9. Incubators 2
Cell suspension culturesShaker 3
.Embryo cultures
4
5

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5
5,5Tween 20.

11. Incubators
ShakerCell suspension culturesEmbryo cultures

12. 1Explant
Murashige1974.
30

13. .
-10-50Clorox0.5-5.255-30
-Tween-80 - Tween-20

14. redox potential
0.5-1
3-5
2-4

15. 2Multiplication Stage:
-
Somatic Cell Embryogenesis
Enhanced Axillary Development
Adventitious shoot Development

16. -Sub culturing

17. 3
In Vitro Rooting and Conditioning:
2-4

18. 4
In Vitro Rooting and Acclimatization
Rooting mixture or Soil mixture

19. 1
2
3
4
5
6
7
somatic Hybridization(
8

20. 1culture of Meristem-tip
2culture of Shoot-tip
3culture of Axillary (lateral) bud
4/Single node culture
5Protoplast Culture
6Cell Suspension Culture
7Onther Culture
8Overview of Callus (Tissue)and Organ Culture

21. (autoclave)

22. (autoclave)

23. (autoclave)

24. Laminar flow air

25. Laminar flow air

26. Laminar flow air

41. Explant
Somatic embryogenesis
Organogenesis
Regenerants
)Plantlets( Callus

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