SlideShare a Scribd company logo
1 of 49
1
Presentation
on
Commercial Exploitation of
Micro-propagation in fruit crops & its Techniques
Presentation
on
Commercial Exploitation of
Micro-propagation in fruit crops & its Techniques
• Course No.:- FsC 505
• Course TiTle:- ProPagaTioN & Nursery
MaNageMeNT For FruiT CroPs
subMiTTed To subMiTTed by
dr. M.M. Masu PawaN KuMar Nagar
assisTaNT researCh sCieNTisT M.sC. (horTi) PoMology
uNiversiTy bhavaN, reg. 04-2690-2015
aau, aNaNd baCa, aau, aNaNd 2
MiCro-ProPagaTioN
3
whaT iswhaT is
MiCroProPagaTioNMiCroProPagaTioNA whole plant can be regenerated from a small
tissue or plant cells in a suitable culture
medium under controlled environment. The
plantlets so produced are called tissue-culture
raised plants.
The advantage is that in a
relatively short time and space
a large number of plants are
obtained.
The advantage is that in a
relatively short time and space
a large number of plants are
obtained.
 TyPe oF MiCroProPagaTioNTyPe oF MiCroProPagaTioN
1.Direct micropropagation
2.Indirect micropropagation
 TyPe oF MiCroProPagaTioNTyPe oF MiCroProPagaTioN
1.Direct micropropagation
2.Indirect micropropagation
4
why dowhy do
MiCroProPagaTioNMiCroProPagaTioN
why dowhy do
MiCroProPagaTioNMiCroProPagaTioN
 A single explant can be multiplied into several thousand plants in less
than a year - this allows fast commercial propagation of new cultivars.
 Once established, a plant tissue culture line can give a continuous supply
of young plants throughout the year.
 In plants prone to virus diseases, virus free explants (new meristem
tissue is usually virus free) can be cultivated to provide virus free plants.
 Plant ‘tissue banks’ can be frozen, then regenerated through tissue
culture.
 Plant cultures in approved media are easier to export than are soil-grown
plants, as they are pathogen free and take up little space (most current
plant export is now done in this manner).
 A single explant can be multiplied into several thousand plants in less
than a year - this allows fast commercial propagation of new cultivars.
 Once established, a plant tissue culture line can give a continuous supply
of young plants throughout the year.
 In plants prone to virus diseases, virus free explants (new meristem
tissue is usually virus free) can be cultivated to provide virus free plants.
 Plant ‘tissue banks’ can be frozen, then regenerated through tissue
culture.
 Plant cultures in approved media are easier to export than are soil-grown
plants, as they are pathogen free and take up little space (most current
plant export is now done in this manner).
5
Advantages of micropropagationAdvantages of micropropagation
 From one to many propagules rapidly
 Multiplication in controlled lab conditions
 Continuous propagation year round
 Potential for disease-free propagules
 Inexpensive per plant once established
 Precise crop production scheduling
 Reduce stock plant space
 Long-term germplasm storage
 Production of difficult-to-propagate species
DisadvantagesDisadvantagesDisadvantagesDisadvantages
 Specialized equipment/facilities required
 More technical expertise required
 Protocols not optimized for all species
 Plants produced may not fit industry standards
 Relatively expensive to set up
6
7
genetically
modified
plant breeding
does not
produce seeds
does not respond
well to vegetative
reproduction8
Steps of MicropropagationSteps of Micropropagation
9
Micropropagation involved in 5 steps:
10
Steps involved in theSteps involved in the in vitroin vitro MicropropagationMicropropagation
Cleaning of glassware
Preparation of nutrient medium
Selection and sterilization of explant.
Inoculation of aseptic explant in to nutrient medium.
Proliferation of shoots on a multiplication medium.
Transfer of shoots for sub-culturing.
Rooting and hardening of plantlets
Field trials.
11
12Source : Cadila pharmaceuticals limited
Stage 0 : Selection and maintenance of stock plants for culture
initiation
EXPLANTEXPLANT
SHOOT TIPS FROM YOUNG SUCKERSHOOT TIPS FROM YOUNG SUCKER
APICAL MERISTEM (1-2 Cm3)APICAL MERISTEM (1-2 Cm3)
SURFACED STERILIZATIONSURFACED STERILIZATION
13
ExplantExplant
Cell, tissue or organ
of a plant that is
used to start in
vitro cultures.
14
15
Stage- I : Initiation and establishment of aseptic culture
16
Source: Jain Irrigation Systems Ltd
17
18
Initiation
19
20
Stage –II : Multiplication of shoot
21
22
Stage -III: Rooting
23
24
Stage –IV: Acclimatization / Hardening
25
 Healthy/elite plantlets are exposed to the natural conditions in a step wise manner.
 It is a gradual acclimatization of in vitro grown plants to in vivo condition.
 The plantlets are transferred to the pots/polyghene bag and immediately irrigated with
inorganic/nutrient solution.
 Plants are kept in the hardening room where controlled conditions of light, humidity
and temperature are maintained.
 Plants are maintained under high humidity for 10-20 days and subsequently
transferred in the field so as to grow under natural conditions. The success rate of
micropropagation depends on the survival of the plantlets when transferred from
culture to the soil (field).
26
Secondary Hardening
27
Plants transferred to nursery bagsPlants transferred to nursery bags
Kept for 6 to 8 weeks under 50% shadeKept for 6 to 8 weeks under 50% shade
Regular foliar spraysRegular foliar sprays
Variation if observed is discardedVariation if observed is discarded
Plant ready for sale (1 feet height)Plant ready for sale (1 feet height)
28
29
Factors Affecting micro-propagation
1. Growth Media
2. Environmental Factors
3. Explants Source
4. Genetics
30
Method of Micro-propagationMethod of Micro-propagation
31
Method of Micro-propagation
A. Meristem tip cultureA. Meristem tip culture
B. Callus cultureB. Callus culture
C. Suspension cultureC. Suspension culture
E. Protoplast cultureE. Protoplast culture
D. Embryo cultureD. Embryo culture
F. Ovule cultureF. Ovule culture
G. Anther cultureG. Anther culture
H. Cell cultureH. Cell culture
32
In addition to major use of tissue culture techniques
for rapid clonal multiplication of plants, this
technique is highly important for several purpose as
under:
1. Production and maintenance of pathogen free stock
plants;
2. Long term in vitro conservation of germplasm.
3. Selection and regeneration of transgenic plants.
4. Conservation of germplasm.
Other Implications of Micro-propagationOther Implications of Micro-propagation
33
Micro-propagation Techniques & ProcedureMicro-propagation Techniques & Procedure
34
A. Micro-propagation by NodesA. Micro-propagation by Nodes
B. Tip Axillary Bud cultureB. Tip Axillary Bud culture
C. Micro-cross Section technologyC. Micro-cross Section technology
G. Micro-propagation by Node cuttings in a liquid
medium
G. Micro-propagation by Node cuttings in a liquid
medium
D. Shoot tip cultureD. Shoot tip culture
E. Seed cultureE. Seed culture
F. Pseudo-bulb segment cultureF. Pseudo-bulb segment culture
35
1. Sterilize Petri dishes and prepare the laminar flow chamber by
disinfecting the internal surfaces with alcohol. Sterilize the tools
with an instrument sterilizer and place them on a sterile dish.
2. Open the tube, take off the plantlet and place it on a Petri dish
with the help of forceps.
3. Remove the leaves and cut the nodes.
4. Open a tube containing fresh sterile medium and place a node
inside, trying to plunge it slightly into the medium with the bud up.
Close the tube.
5. Seal the tube with a gas-permeable plastic tape and label it
correctly.
It is recommended to place two explants in 16 x 125 mm tubes,
three in 18 x 150 mm tubes, five in 25 x 150 mm tubes, and 20-30
in magenta vessels.
1. Sterilize Petri dishes and prepare the laminar flow chamber by
disinfecting the internal surfaces with alcohol. Sterilize the tools
with an instrument sterilizer and place them on a sterile dish.
2. Open the tube, take off the plantlet and place it on a Petri dish
with the help of forceps.
3. Remove the leaves and cut the nodes.
4. Open a tube containing fresh sterile medium and place a node
inside, trying to plunge it slightly into the medium with the bud up.
Close the tube.
5. Seal the tube with a gas-permeable plastic tape and label it
correctly.
It is recommended to place two explants in 16 x 125 mm tubes,
three in 18 x 150 mm tubes, five in 25 x 150 mm tubes, and 20-30
in magenta vessels.
procedureprocedure
Applications of Tissue Culture
1. Embryo culture
2. Meristem culture
3. Micropropagation
4. Somatic embryogenesis and Organogenesis
5. Somaclonal variation and in vitro selection
6. Anther culture Haploid & Dihaploid Production
7. Protoplast culture (In vitro hybridization –
Protoplast Fusion)
8. Germplasm preservation
36
Micro-propagation technology developed
for some Horticultural Crops in India
Sr.
No.
Crop Institute
1. Annona IIHR, Banglore; BARC, Mumbai; NCL,
Pune
2. Banana NCL, Pune; IIHR, Banglore; TNAU,
Coimbatore
3. Citrus NBRI, Lucknow; NRC- Citrus, Nagpur
4. Grape IARI, New Delhi
5. Guava GBPUAT, Pantnagar
6. Papaya IARI, New Delhi
7. Pineapple BARC, Mumbai
8. Strawberry TERI, New Delhi
37
38
Tissue culture BananaTissue culture Banana
39
Tissue culTure DaTe palm
40
Tissue culTure pomegranaTeTissue culTure pomegranaTe
41
TissueTissue culTureculTure papayapapaya
42
Tissue culTure sTrawberry
43
44
clonal- propagaTion in guavaclonal- propagaTion in guava
45
micro- propagaTion in aonlamicro- propagaTion in aonla
46
micro- propagaTion in Jamunmicro- propagaTion in Jamun
47
micro- propagaTion in bealmicro- propagaTion in beal
48
micro- propagaTion in Figmicro- propagaTion in Fig
49

More Related Content

What's hot

Seed Health Testing
Seed Health TestingSeed Health Testing
Seed Health TestingSeeds
 
Isolation distance IN SEED PRODUCTION
Isolation distance IN SEED PRODUCTIONIsolation distance IN SEED PRODUCTION
Isolation distance IN SEED PRODUCTIONMahammed Faizan
 
principles of seed production
principles of seed productionprinciples of seed production
principles of seed productionHARISH J
 
Floral biology of important cucurbits
Floral biology of important cucurbitsFloral biology of important cucurbits
Floral biology of important cucurbitsAvisha Budhani
 
Seed certification and type of losses caused by seed borne diseases in true a...
Seed certification and type of losses caused by seed borne diseases in true a...Seed certification and type of losses caused by seed borne diseases in true a...
Seed certification and type of losses caused by seed borne diseases in true a...Summer
 
Seed Generation system.pdf
Seed Generation system.pdfSeed Generation system.pdf
Seed Generation system.pdfVanangamudiK1
 
Advances breeding of Papaya
 Advances breeding of Papaya Advances breeding of Papaya
Advances breeding of PapayaGANGARAM RANA
 
Berseem: Fodder Crop
Berseem: Fodder CropBerseem: Fodder Crop
Berseem: Fodder CropYuvraj Singh
 
Hybrid seed production in castor and maize
Hybrid seed production in castor and maizeHybrid seed production in castor and maize
Hybrid seed production in castor and maizeRAHULCHANDERA1
 
Pl. PATH-605 Introduction to certification. International scenario of certifi...
Pl. PATH-605 Introduction to certification. International scenario of certifi...Pl. PATH-605 Introduction to certification. International scenario of certifi...
Pl. PATH-605 Introduction to certification. International scenario of certifi...Harshvardhan Gaikwad
 
release and notification of variety in india
release and notification of variety in indiarelease and notification of variety in india
release and notification of variety in indiaMahammed Faizan
 
Hybrid seed technology
Hybrid seed technology Hybrid seed technology
Hybrid seed technology mirzausman555
 
Male Sterility in Pigeon pea
Male Sterility in Pigeon peaMale Sterility in Pigeon pea
Male Sterility in Pigeon peaDhaval Bhanderi
 

What's hot (20)

cucurbits seed production
cucurbits seed productioncucurbits seed production
cucurbits seed production
 
Seed Health Testing
Seed Health TestingSeed Health Testing
Seed Health Testing
 
Isolation distance IN SEED PRODUCTION
Isolation distance IN SEED PRODUCTIONIsolation distance IN SEED PRODUCTION
Isolation distance IN SEED PRODUCTION
 
principles of seed production
principles of seed productionprinciples of seed production
principles of seed production
 
Jaydev Kumar Upadhyay
Jaydev Kumar UpadhyayJaydev Kumar Upadhyay
Jaydev Kumar Upadhyay
 
Breeding of Maize
Breeding of MaizeBreeding of Maize
Breeding of Maize
 
Floral biology of important cucurbits
Floral biology of important cucurbitsFloral biology of important cucurbits
Floral biology of important cucurbits
 
Seed certification and type of losses caused by seed borne diseases in true a...
Seed certification and type of losses caused by seed borne diseases in true a...Seed certification and type of losses caused by seed borne diseases in true a...
Seed certification and type of losses caused by seed borne diseases in true a...
 
Seed Generation system.pdf
Seed Generation system.pdfSeed Generation system.pdf
Seed Generation system.pdf
 
Brinjal
BrinjalBrinjal
Brinjal
 
Advances breeding of Papaya
 Advances breeding of Papaya Advances breeding of Papaya
Advances breeding of Papaya
 
Berseem: Fodder Crop
Berseem: Fodder CropBerseem: Fodder Crop
Berseem: Fodder Crop
 
Hybrid seed production in castor and maize
Hybrid seed production in castor and maizeHybrid seed production in castor and maize
Hybrid seed production in castor and maize
 
Seed testing of laboratry
Seed testing of laboratrySeed testing of laboratry
Seed testing of laboratry
 
Seed processing and storage
Seed processing and storageSeed processing and storage
Seed processing and storage
 
Pl. PATH-605 Introduction to certification. International scenario of certifi...
Pl. PATH-605 Introduction to certification. International scenario of certifi...Pl. PATH-605 Introduction to certification. International scenario of certifi...
Pl. PATH-605 Introduction to certification. International scenario of certifi...
 
release and notification of variety in india
release and notification of variety in indiarelease and notification of variety in india
release and notification of variety in india
 
Hybrid seed production of pigeonpea
Hybrid seed production of pigeonpea Hybrid seed production of pigeonpea
Hybrid seed production of pigeonpea
 
Hybrid seed technology
Hybrid seed technology Hybrid seed technology
Hybrid seed technology
 
Male Sterility in Pigeon pea
Male Sterility in Pigeon peaMale Sterility in Pigeon pea
Male Sterility in Pigeon pea
 

Viewers also liked

Commercial exploitation of micropropagation& its tech in fruit crops
Commercial exploitation of micropropagation& its tech in fruit cropsCommercial exploitation of micropropagation& its tech in fruit crops
Commercial exploitation of micropropagation& its tech in fruit cropsPawan Nagar
 
Commercial exploitation of micro its technology
Commercial exploitation of micro its technologyCommercial exploitation of micro its technology
Commercial exploitation of micro its technologyPawan Nagar
 
Recent advances and commercial propagation technique in fruit crops
Recent advances and commercial propagation technique in fruit cropsRecent advances and commercial propagation technique in fruit crops
Recent advances and commercial propagation technique in fruit cropsPawan Nagar
 
Clonal propagation tissueculture
Clonal propagation tissuecultureClonal propagation tissueculture
Clonal propagation tissuecultureReal Creations
 
Micropropagation. adil.
Micropropagation. adil.Micropropagation. adil.
Micropropagation. adil.Adil Mazeed
 
Techniques of in vitro clonal propagation for fruit crops
Techniques of in vitro  clonal propagation for fruit cropsTechniques of in vitro  clonal propagation for fruit crops
Techniques of in vitro clonal propagation for fruit cropsPawan Nagar
 
Laurens et al., 2010. the new eu project fruit breedomics an approach for inc...
Laurens et al., 2010. the new eu project fruit breedomics an approach for inc...Laurens et al., 2010. the new eu project fruit breedomics an approach for inc...
Laurens et al., 2010. the new eu project fruit breedomics an approach for inc...fruitbreedomics
 
In situ/On farm Conservation and Use of Agricultural Biodiversity (Horticultu...
In situ/On farm Conservation and Use of Agricultural Biodiversity (Horticultu...In situ/On farm Conservation and Use of Agricultural Biodiversity (Horticultu...
In situ/On farm Conservation and Use of Agricultural Biodiversity (Horticultu...Bioversity International
 
Varietal improvement and conservation the economics of orchard renewal - da...
Varietal improvement and conservation   the economics of orchard renewal - da...Varietal improvement and conservation   the economics of orchard renewal - da...
Varietal improvement and conservation the economics of orchard renewal - da...MacadamiaSociety
 
Impacts of climate change on important fruit crops of Rosaceae family
Impacts of climate change on important fruit crops of Rosaceae familyImpacts of climate change on important fruit crops of Rosaceae family
Impacts of climate change on important fruit crops of Rosaceae familyChongtham Allaylay Devi
 
Area & production, productivity,export potential,and national scenario of fru...
Area & production, productivity,export potential,and national scenario of fru...Area & production, productivity,export potential,and national scenario of fru...
Area & production, productivity,export potential,and national scenario of fru...Delince Samuel
 
Propagation of cardamom
Propagation of cardamomPropagation of cardamom
Propagation of cardamomVishnu Mishra
 
abiotic stress and its management in fruit crops
abiotic stress and its management in fruit cropsabiotic stress and its management in fruit crops
abiotic stress and its management in fruit cropsrehana javid
 
Cultivation practice of Arabian Date Palm P. dactylifera
Cultivation practice of Arabian Date Palm P. dactyliferaCultivation practice of Arabian Date Palm P. dactylifera
Cultivation practice of Arabian Date Palm P. dactyliferaMd. Nazim Uddin Mondal
 
Fruit breeding method- introduction(IN INDIA)- Panchaal B.
Fruit breeding method- introduction(IN INDIA)- Panchaal B.Fruit breeding method- introduction(IN INDIA)- Panchaal B.
Fruit breeding method- introduction(IN INDIA)- Panchaal B.Panchaal Bhattacharjee
 

Viewers also liked (20)

Commercial exploitation of micropropagation& its tech in fruit crops
Commercial exploitation of micropropagation& its tech in fruit cropsCommercial exploitation of micropropagation& its tech in fruit crops
Commercial exploitation of micropropagation& its tech in fruit crops
 
Commercial exploitation of micro its technology
Commercial exploitation of micro its technologyCommercial exploitation of micro its technology
Commercial exploitation of micro its technology
 
Shwetha
ShwethaShwetha
Shwetha
 
Recent advances and commercial propagation technique in fruit crops
Recent advances and commercial propagation technique in fruit cropsRecent advances and commercial propagation technique in fruit crops
Recent advances and commercial propagation technique in fruit crops
 
Clonal propagation tissueculture
Clonal propagation tissuecultureClonal propagation tissueculture
Clonal propagation tissueculture
 
Micropropagation. adil.
Micropropagation. adil.Micropropagation. adil.
Micropropagation. adil.
 
Techniques of in vitro clonal propagation for fruit crops
Techniques of in vitro  clonal propagation for fruit cropsTechniques of in vitro  clonal propagation for fruit crops
Techniques of in vitro clonal propagation for fruit crops
 
Laurens et al., 2010. the new eu project fruit breedomics an approach for inc...
Laurens et al., 2010. the new eu project fruit breedomics an approach for inc...Laurens et al., 2010. the new eu project fruit breedomics an approach for inc...
Laurens et al., 2010. the new eu project fruit breedomics an approach for inc...
 
Climate Resilient Horticultural Crops of Future
Climate Resilient Horticultural Crops of FutureClimate Resilient Horticultural Crops of Future
Climate Resilient Horticultural Crops of Future
 
In situ/On farm Conservation and Use of Agricultural Biodiversity (Horticultu...
In situ/On farm Conservation and Use of Agricultural Biodiversity (Horticultu...In situ/On farm Conservation and Use of Agricultural Biodiversity (Horticultu...
In situ/On farm Conservation and Use of Agricultural Biodiversity (Horticultu...
 
Varietal improvement and conservation the economics of orchard renewal - da...
Varietal improvement and conservation   the economics of orchard renewal - da...Varietal improvement and conservation   the economics of orchard renewal - da...
Varietal improvement and conservation the economics of orchard renewal - da...
 
Iraq
IraqIraq
Iraq
 
Underutilized fruit crops
Underutilized fruit cropsUnderutilized fruit crops
Underutilized fruit crops
 
Impacts of climate change on important fruit crops of Rosaceae family
Impacts of climate change on important fruit crops of Rosaceae familyImpacts of climate change on important fruit crops of Rosaceae family
Impacts of climate change on important fruit crops of Rosaceae family
 
Area & production, productivity,export potential,and national scenario of fru...
Area & production, productivity,export potential,and national scenario of fru...Area & production, productivity,export potential,and national scenario of fru...
Area & production, productivity,export potential,and national scenario of fru...
 
Propagation of cardamom
Propagation of cardamomPropagation of cardamom
Propagation of cardamom
 
Bael presentation
Bael presentationBael presentation
Bael presentation
 
abiotic stress and its management in fruit crops
abiotic stress and its management in fruit cropsabiotic stress and its management in fruit crops
abiotic stress and its management in fruit crops
 
Cultivation practice of Arabian Date Palm P. dactylifera
Cultivation practice of Arabian Date Palm P. dactyliferaCultivation practice of Arabian Date Palm P. dactylifera
Cultivation practice of Arabian Date Palm P. dactylifera
 
Fruit breeding method- introduction(IN INDIA)- Panchaal B.
Fruit breeding method- introduction(IN INDIA)- Panchaal B.Fruit breeding method- introduction(IN INDIA)- Panchaal B.
Fruit breeding method- introduction(IN INDIA)- Panchaal B.
 

Similar to Commercial Exploitation of Micro-propagation in fruit crops & its Techniques

4.2 Micropropagation-Concept and techniques3.pptx
4.2 Micropropagation-Concept and techniques3.pptx4.2 Micropropagation-Concept and techniques3.pptx
4.2 Micropropagation-Concept and techniques3.pptxYoGeshSharma834784
 
citrus.pptx ent jvjvguzugfuffugcgchcyfhvhv
citrus.pptx ent jvjvguzugfuffugcgchcyfhvhvcitrus.pptx ent jvjvguzugfuffugcgchcyfhvhv
citrus.pptx ent jvjvguzugfuffugcgchcyfhvhvshashankshashank3736
 
PLANT TISSUE CULTURE.pptx
PLANT TISSUE CULTURE.pptxPLANT TISSUE CULTURE.pptx
PLANT TISSUE CULTURE.pptxVanangamudiK1
 
Micropropagation.pptx
Micropropagation.pptxMicropropagation.pptx
Micropropagation.pptxRenuJangid3
 
Lecture 13 & 14 (student copy).pptx
Lecture 13 & 14 (student copy).pptxLecture 13 & 14 (student copy).pptx
Lecture 13 & 14 (student copy).pptxSarahStephenie2
 
Plant tissue culture ⅱ
Plant tissue culture  ⅱPlant tissue culture  ⅱ
Plant tissue culture ⅱbhoomishah45
 
Plant tissue culture techniques
Plant tissue culture techniquesPlant tissue culture techniques
Plant tissue culture techniquesArvind Yadav
 
Micropropagation and commercial exploitation in horticulture crops
Micropropagation and commercial exploitation in horticulture cropsMicropropagation and commercial exploitation in horticulture crops
Micropropagation and commercial exploitation in horticulture cropsDheeraj Sharma
 
Presentation1 rishu micropropagation.pptx
Presentation1 rishu micropropagation.pptxPresentation1 rishu micropropagation.pptx
Presentation1 rishu micropropagation.pptxanandanandbhai2017
 
Presentation1 rishu micropropagation.pptx
Presentation1 rishu micropropagation.pptxPresentation1 rishu micropropagation.pptx
Presentation1 rishu micropropagation.pptxanandanandbhai2017
 
Clonal Propagation: Introduction, Techniques, Factors, Applications and Disad...
Clonal Propagation: Introduction, Techniques, Factors, Applications and Disad...Clonal Propagation: Introduction, Techniques, Factors, Applications and Disad...
Clonal Propagation: Introduction, Techniques, Factors, Applications and Disad...A Biodiction : A Unit of Dr. Divya Sharma
 
Meristem and shoot tip culture in horticultural crops
Meristem and shoot tip culture in horticultural cropsMeristem and shoot tip culture in horticultural crops
Meristem and shoot tip culture in horticultural cropsHORTIPEDIA INDIA
 

Similar to Commercial Exploitation of Micro-propagation in fruit crops & its Techniques (20)

Micropropagation
MicropropagationMicropropagation
Micropropagation
 
Tissue culture
Tissue cultureTissue culture
Tissue culture
 
4.2 Micropropagation-Concept and techniques3.pptx
4.2 Micropropagation-Concept and techniques3.pptx4.2 Micropropagation-Concept and techniques3.pptx
4.2 Micropropagation-Concept and techniques3.pptx
 
Plant tissue culture pharmacongosy-1 Semester 4
Plant tissue culture pharmacongosy-1 Semester 4Plant tissue culture pharmacongosy-1 Semester 4
Plant tissue culture pharmacongosy-1 Semester 4
 
citrus.pptx ent jvjvguzugfuffugcgchcyfhvhv
citrus.pptx ent jvjvguzugfuffugcgchcyfhvhvcitrus.pptx ent jvjvguzugfuffugcgchcyfhvhv
citrus.pptx ent jvjvguzugfuffugcgchcyfhvhv
 
PLANT TISSUE CULTURE.pptx
PLANT TISSUE CULTURE.pptxPLANT TISSUE CULTURE.pptx
PLANT TISSUE CULTURE.pptx
 
Micropropagation
MicropropagationMicropropagation
Micropropagation
 
Micropropagation.pptx
Micropropagation.pptxMicropropagation.pptx
Micropropagation.pptx
 
Plant Tissue Culture
Plant Tissue Culture Plant Tissue Culture
Plant Tissue Culture
 
Lecture 13 & 14 (student copy).pptx
Lecture 13 & 14 (student copy).pptxLecture 13 & 14 (student copy).pptx
Lecture 13 & 14 (student copy).pptx
 
Plant tissue culture ⅱ
Plant tissue culture  ⅱPlant tissue culture  ⅱ
Plant tissue culture ⅱ
 
Plant tissue culture
Plant tissue culturePlant tissue culture
Plant tissue culture
 
Plant tissue culture techniques
Plant tissue culture techniquesPlant tissue culture techniques
Plant tissue culture techniques
 
Micropropagation
MicropropagationMicropropagation
Micropropagation
 
Micropropagation
MicropropagationMicropropagation
Micropropagation
 
Micropropagation and commercial exploitation in horticulture crops
Micropropagation and commercial exploitation in horticulture cropsMicropropagation and commercial exploitation in horticulture crops
Micropropagation and commercial exploitation in horticulture crops
 
Presentation1 rishu micropropagation.pptx
Presentation1 rishu micropropagation.pptxPresentation1 rishu micropropagation.pptx
Presentation1 rishu micropropagation.pptx
 
Presentation1 rishu micropropagation.pptx
Presentation1 rishu micropropagation.pptxPresentation1 rishu micropropagation.pptx
Presentation1 rishu micropropagation.pptx
 
Clonal Propagation: Introduction, Techniques, Factors, Applications and Disad...
Clonal Propagation: Introduction, Techniques, Factors, Applications and Disad...Clonal Propagation: Introduction, Techniques, Factors, Applications and Disad...
Clonal Propagation: Introduction, Techniques, Factors, Applications and Disad...
 
Meristem and shoot tip culture in horticultural crops
Meristem and shoot tip culture in horticultural cropsMeristem and shoot tip culture in horticultural crops
Meristem and shoot tip culture in horticultural crops
 

More from Pawan Nagar

Seminar (Pawan Kumar Nagar)
Seminar (Pawan Kumar Nagar)Seminar (Pawan Kumar Nagar)
Seminar (Pawan Kumar Nagar)Pawan Nagar
 
Advanced production technology of wood apple
Advanced  production technology of wood appleAdvanced  production technology of wood apple
Advanced production technology of wood applePawan Nagar
 
Advanced production technology of walnut
Advanced  production technology of walnutAdvanced  production technology of walnut
Advanced production technology of walnutPawan Nagar
 
Advanced production technology of pear
Advanced  production technology of pearAdvanced  production technology of pear
Advanced production technology of pearPawan Nagar
 
Advanced production technology of peach
Advanced  production technology of peachAdvanced  production technology of peach
Advanced production technology of peachPawan Nagar
 
Advanced production technology of mangosteen
Advanced  production technology of mangosteenAdvanced  production technology of mangosteen
Advanced production technology of mangosteenPawan Nagar
 
Advanced production technology of litchi
Advanced  production technology of litchiAdvanced  production technology of litchi
Advanced production technology of litchiPawan Nagar
 
Advanced production technology of kiwi fruit
Advanced  production technology of kiwi fruitAdvanced  production technology of kiwi fruit
Advanced production technology of kiwi fruitPawan Nagar
 
Advanced production technology of jamun crop
Advanced  production technology of jamun cropAdvanced  production technology of jamun crop
Advanced production technology of jamun cropPawan Nagar
 
Advanced production technology of bael
Advanced  production technology of baelAdvanced  production technology of bael
Advanced production technology of baelPawan Nagar
 
Advanced production technology of almond
Advanced  production technology of almondAdvanced  production technology of almond
Advanced production technology of almondPawan Nagar
 
Principle & different method of cutting & layering]
Principle & different method of cutting & layering]Principle & different method of cutting & layering]
Principle & different method of cutting & layering]Pawan Nagar
 
Factor affecting of seed germination
Factor affecting of seed germinationFactor affecting of seed germination
Factor affecting of seed germinationPawan Nagar
 
Direct organogenesis, embryogenesis, micro grafting, meristem culture and its...
Direct organogenesis, embryogenesis, micro grafting, meristem culture and its...Direct organogenesis, embryogenesis, micro grafting, meristem culture and its...
Direct organogenesis, embryogenesis, micro grafting, meristem culture and its...Pawan Nagar
 
Direct organogenesis, embryogenesis, micro grafting, meristem culture and its...
Direct organogenesis, embryogenesis, micro grafting, meristem culture and its...Direct organogenesis, embryogenesis, micro grafting, meristem culture and its...
Direct organogenesis, embryogenesis, micro grafting, meristem culture and its...Pawan Nagar
 
walnut production technology
walnut production technologywalnut production technology
walnut production technologyPawan Nagar
 
Harvesting practices for special market purpose
Harvesting practices for special market purposeHarvesting practices for special market purpose
Harvesting practices for special market purposePawan Nagar
 
Synthetic and composite variety
Synthetic and composite varietySynthetic and composite variety
Synthetic and composite varietyPawan Nagar
 

More from Pawan Nagar (20)

Canopy
CanopyCanopy
Canopy
 
Canopy
CanopyCanopy
Canopy
 
Seminar (Pawan Kumar Nagar)
Seminar (Pawan Kumar Nagar)Seminar (Pawan Kumar Nagar)
Seminar (Pawan Kumar Nagar)
 
Advanced production technology of wood apple
Advanced  production technology of wood appleAdvanced  production technology of wood apple
Advanced production technology of wood apple
 
Advanced production technology of walnut
Advanced  production technology of walnutAdvanced  production technology of walnut
Advanced production technology of walnut
 
Advanced production technology of pear
Advanced  production technology of pearAdvanced  production technology of pear
Advanced production technology of pear
 
Advanced production technology of peach
Advanced  production technology of peachAdvanced  production technology of peach
Advanced production technology of peach
 
Advanced production technology of mangosteen
Advanced  production technology of mangosteenAdvanced  production technology of mangosteen
Advanced production technology of mangosteen
 
Advanced production technology of litchi
Advanced  production technology of litchiAdvanced  production technology of litchi
Advanced production technology of litchi
 
Advanced production technology of kiwi fruit
Advanced  production technology of kiwi fruitAdvanced  production technology of kiwi fruit
Advanced production technology of kiwi fruit
 
Advanced production technology of jamun crop
Advanced  production technology of jamun cropAdvanced  production technology of jamun crop
Advanced production technology of jamun crop
 
Advanced production technology of bael
Advanced  production technology of baelAdvanced  production technology of bael
Advanced production technology of bael
 
Advanced production technology of almond
Advanced  production technology of almondAdvanced  production technology of almond
Advanced production technology of almond
 
Principle & different method of cutting & layering]
Principle & different method of cutting & layering]Principle & different method of cutting & layering]
Principle & different method of cutting & layering]
 
Factor affecting of seed germination
Factor affecting of seed germinationFactor affecting of seed germination
Factor affecting of seed germination
 
Direct organogenesis, embryogenesis, micro grafting, meristem culture and its...
Direct organogenesis, embryogenesis, micro grafting, meristem culture and its...Direct organogenesis, embryogenesis, micro grafting, meristem culture and its...
Direct organogenesis, embryogenesis, micro grafting, meristem culture and its...
 
Direct organogenesis, embryogenesis, micro grafting, meristem culture and its...
Direct organogenesis, embryogenesis, micro grafting, meristem culture and its...Direct organogenesis, embryogenesis, micro grafting, meristem culture and its...
Direct organogenesis, embryogenesis, micro grafting, meristem culture and its...
 
walnut production technology
walnut production technologywalnut production technology
walnut production technology
 
Harvesting practices for special market purpose
Harvesting practices for special market purposeHarvesting practices for special market purpose
Harvesting practices for special market purpose
 
Synthetic and composite variety
Synthetic and composite varietySynthetic and composite variety
Synthetic and composite variety
 

Recently uploaded

Historical philosophical, theoretical, and legal foundations of special and i...
Historical philosophical, theoretical, and legal foundations of special and i...Historical philosophical, theoretical, and legal foundations of special and i...
Historical philosophical, theoretical, and legal foundations of special and i...jaredbarbolino94
 
ECONOMIC CONTEXT - LONG FORM TV DRAMA - PPT
ECONOMIC CONTEXT - LONG FORM TV DRAMA - PPTECONOMIC CONTEXT - LONG FORM TV DRAMA - PPT
ECONOMIC CONTEXT - LONG FORM TV DRAMA - PPTiammrhaywood
 
Presiding Officer Training module 2024 lok sabha elections
Presiding Officer Training module 2024 lok sabha electionsPresiding Officer Training module 2024 lok sabha elections
Presiding Officer Training module 2024 lok sabha electionsanshu789521
 
Computed Fields and api Depends in the Odoo 17
Computed Fields and api Depends in the Odoo 17Computed Fields and api Depends in the Odoo 17
Computed Fields and api Depends in the Odoo 17Celine George
 
Solving Puzzles Benefits Everyone (English).pptx
Solving Puzzles Benefits Everyone (English).pptxSolving Puzzles Benefits Everyone (English).pptx
Solving Puzzles Benefits Everyone (English).pptxOH TEIK BIN
 
Meghan Sutherland In Media Res Media Component
Meghan Sutherland In Media Res Media ComponentMeghan Sutherland In Media Res Media Component
Meghan Sutherland In Media Res Media ComponentInMediaRes1
 
Alper Gobel In Media Res Media Component
Alper Gobel In Media Res Media ComponentAlper Gobel In Media Res Media Component
Alper Gobel In Media Res Media ComponentInMediaRes1
 
What is Model Inheritance in Odoo 17 ERP
What is Model Inheritance in Odoo 17 ERPWhat is Model Inheritance in Odoo 17 ERP
What is Model Inheritance in Odoo 17 ERPCeline George
 
Blooming Together_ Growing a Community Garden Worksheet.docx
Blooming Together_ Growing a Community Garden Worksheet.docxBlooming Together_ Growing a Community Garden Worksheet.docx
Blooming Together_ Growing a Community Garden Worksheet.docxUnboundStockton
 
Organic Name Reactions for the students and aspirants of Chemistry12th.pptx
Organic Name Reactions  for the students and aspirants of Chemistry12th.pptxOrganic Name Reactions  for the students and aspirants of Chemistry12th.pptx
Organic Name Reactions for the students and aspirants of Chemistry12th.pptxVS Mahajan Coaching Centre
 
Like-prefer-love -hate+verb+ing & silent letters & citizenship text.pdf
Like-prefer-love -hate+verb+ing & silent letters & citizenship text.pdfLike-prefer-love -hate+verb+ing & silent letters & citizenship text.pdf
Like-prefer-love -hate+verb+ing & silent letters & citizenship text.pdfMr Bounab Samir
 
Introduction to AI in Higher Education_draft.pptx
Introduction to AI in Higher Education_draft.pptxIntroduction to AI in Higher Education_draft.pptx
Introduction to AI in Higher Education_draft.pptxpboyjonauth
 
Framing an Appropriate Research Question 6b9b26d93da94caf993c038d9efcdedb.pdf
Framing an Appropriate Research Question 6b9b26d93da94caf993c038d9efcdedb.pdfFraming an Appropriate Research Question 6b9b26d93da94caf993c038d9efcdedb.pdf
Framing an Appropriate Research Question 6b9b26d93da94caf993c038d9efcdedb.pdfUjwalaBharambe
 
Introduction to ArtificiaI Intelligence in Higher Education
Introduction to ArtificiaI Intelligence in Higher EducationIntroduction to ArtificiaI Intelligence in Higher Education
Introduction to ArtificiaI Intelligence in Higher Educationpboyjonauth
 
POINT- BIOCHEMISTRY SEM 2 ENZYMES UNIT 5.pptx
POINT- BIOCHEMISTRY SEM 2 ENZYMES UNIT 5.pptxPOINT- BIOCHEMISTRY SEM 2 ENZYMES UNIT 5.pptx
POINT- BIOCHEMISTRY SEM 2 ENZYMES UNIT 5.pptxSayali Powar
 
Roles & Responsibilities in Pharmacovigilance
Roles & Responsibilities in PharmacovigilanceRoles & Responsibilities in Pharmacovigilance
Roles & Responsibilities in PharmacovigilanceSamikshaHamane
 

Recently uploaded (20)

Historical philosophical, theoretical, and legal foundations of special and i...
Historical philosophical, theoretical, and legal foundations of special and i...Historical philosophical, theoretical, and legal foundations of special and i...
Historical philosophical, theoretical, and legal foundations of special and i...
 
ECONOMIC CONTEXT - LONG FORM TV DRAMA - PPT
ECONOMIC CONTEXT - LONG FORM TV DRAMA - PPTECONOMIC CONTEXT - LONG FORM TV DRAMA - PPT
ECONOMIC CONTEXT - LONG FORM TV DRAMA - PPT
 
Presiding Officer Training module 2024 lok sabha elections
Presiding Officer Training module 2024 lok sabha electionsPresiding Officer Training module 2024 lok sabha elections
Presiding Officer Training module 2024 lok sabha elections
 
Computed Fields and api Depends in the Odoo 17
Computed Fields and api Depends in the Odoo 17Computed Fields and api Depends in the Odoo 17
Computed Fields and api Depends in the Odoo 17
 
TataKelola dan KamSiber Kecerdasan Buatan v022.pdf
TataKelola dan KamSiber Kecerdasan Buatan v022.pdfTataKelola dan KamSiber Kecerdasan Buatan v022.pdf
TataKelola dan KamSiber Kecerdasan Buatan v022.pdf
 
Solving Puzzles Benefits Everyone (English).pptx
Solving Puzzles Benefits Everyone (English).pptxSolving Puzzles Benefits Everyone (English).pptx
Solving Puzzles Benefits Everyone (English).pptx
 
Meghan Sutherland In Media Res Media Component
Meghan Sutherland In Media Res Media ComponentMeghan Sutherland In Media Res Media Component
Meghan Sutherland In Media Res Media Component
 
9953330565 Low Rate Call Girls In Rohini Delhi NCR
9953330565 Low Rate Call Girls In Rohini  Delhi NCR9953330565 Low Rate Call Girls In Rohini  Delhi NCR
9953330565 Low Rate Call Girls In Rohini Delhi NCR
 
ESSENTIAL of (CS/IT/IS) class 06 (database)
ESSENTIAL of (CS/IT/IS) class 06 (database)ESSENTIAL of (CS/IT/IS) class 06 (database)
ESSENTIAL of (CS/IT/IS) class 06 (database)
 
Alper Gobel In Media Res Media Component
Alper Gobel In Media Res Media ComponentAlper Gobel In Media Res Media Component
Alper Gobel In Media Res Media Component
 
Model Call Girl in Bikash Puri Delhi reach out to us at 🔝9953056974🔝
Model Call Girl in Bikash Puri  Delhi reach out to us at 🔝9953056974🔝Model Call Girl in Bikash Puri  Delhi reach out to us at 🔝9953056974🔝
Model Call Girl in Bikash Puri Delhi reach out to us at 🔝9953056974🔝
 
What is Model Inheritance in Odoo 17 ERP
What is Model Inheritance in Odoo 17 ERPWhat is Model Inheritance in Odoo 17 ERP
What is Model Inheritance in Odoo 17 ERP
 
Blooming Together_ Growing a Community Garden Worksheet.docx
Blooming Together_ Growing a Community Garden Worksheet.docxBlooming Together_ Growing a Community Garden Worksheet.docx
Blooming Together_ Growing a Community Garden Worksheet.docx
 
Organic Name Reactions for the students and aspirants of Chemistry12th.pptx
Organic Name Reactions  for the students and aspirants of Chemistry12th.pptxOrganic Name Reactions  for the students and aspirants of Chemistry12th.pptx
Organic Name Reactions for the students and aspirants of Chemistry12th.pptx
 
Like-prefer-love -hate+verb+ing & silent letters & citizenship text.pdf
Like-prefer-love -hate+verb+ing & silent letters & citizenship text.pdfLike-prefer-love -hate+verb+ing & silent letters & citizenship text.pdf
Like-prefer-love -hate+verb+ing & silent letters & citizenship text.pdf
 
Introduction to AI in Higher Education_draft.pptx
Introduction to AI in Higher Education_draft.pptxIntroduction to AI in Higher Education_draft.pptx
Introduction to AI in Higher Education_draft.pptx
 
Framing an Appropriate Research Question 6b9b26d93da94caf993c038d9efcdedb.pdf
Framing an Appropriate Research Question 6b9b26d93da94caf993c038d9efcdedb.pdfFraming an Appropriate Research Question 6b9b26d93da94caf993c038d9efcdedb.pdf
Framing an Appropriate Research Question 6b9b26d93da94caf993c038d9efcdedb.pdf
 
Introduction to ArtificiaI Intelligence in Higher Education
Introduction to ArtificiaI Intelligence in Higher EducationIntroduction to ArtificiaI Intelligence in Higher Education
Introduction to ArtificiaI Intelligence in Higher Education
 
POINT- BIOCHEMISTRY SEM 2 ENZYMES UNIT 5.pptx
POINT- BIOCHEMISTRY SEM 2 ENZYMES UNIT 5.pptxPOINT- BIOCHEMISTRY SEM 2 ENZYMES UNIT 5.pptx
POINT- BIOCHEMISTRY SEM 2 ENZYMES UNIT 5.pptx
 
Roles & Responsibilities in Pharmacovigilance
Roles & Responsibilities in PharmacovigilanceRoles & Responsibilities in Pharmacovigilance
Roles & Responsibilities in Pharmacovigilance
 

Commercial Exploitation of Micro-propagation in fruit crops & its Techniques

  • 1. 1
  • 2. Presentation on Commercial Exploitation of Micro-propagation in fruit crops & its Techniques Presentation on Commercial Exploitation of Micro-propagation in fruit crops & its Techniques • Course No.:- FsC 505 • Course TiTle:- ProPagaTioN & Nursery MaNageMeNT For FruiT CroPs subMiTTed To subMiTTed by dr. M.M. Masu PawaN KuMar Nagar assisTaNT researCh sCieNTisT M.sC. (horTi) PoMology uNiversiTy bhavaN, reg. 04-2690-2015 aau, aNaNd baCa, aau, aNaNd 2
  • 4. whaT iswhaT is MiCroProPagaTioNMiCroProPagaTioNA whole plant can be regenerated from a small tissue or plant cells in a suitable culture medium under controlled environment. The plantlets so produced are called tissue-culture raised plants. The advantage is that in a relatively short time and space a large number of plants are obtained. The advantage is that in a relatively short time and space a large number of plants are obtained.  TyPe oF MiCroProPagaTioNTyPe oF MiCroProPagaTioN 1.Direct micropropagation 2.Indirect micropropagation  TyPe oF MiCroProPagaTioNTyPe oF MiCroProPagaTioN 1.Direct micropropagation 2.Indirect micropropagation 4
  • 5. why dowhy do MiCroProPagaTioNMiCroProPagaTioN why dowhy do MiCroProPagaTioNMiCroProPagaTioN  A single explant can be multiplied into several thousand plants in less than a year - this allows fast commercial propagation of new cultivars.  Once established, a plant tissue culture line can give a continuous supply of young plants throughout the year.  In plants prone to virus diseases, virus free explants (new meristem tissue is usually virus free) can be cultivated to provide virus free plants.  Plant ‘tissue banks’ can be frozen, then regenerated through tissue culture.  Plant cultures in approved media are easier to export than are soil-grown plants, as they are pathogen free and take up little space (most current plant export is now done in this manner).  A single explant can be multiplied into several thousand plants in less than a year - this allows fast commercial propagation of new cultivars.  Once established, a plant tissue culture line can give a continuous supply of young plants throughout the year.  In plants prone to virus diseases, virus free explants (new meristem tissue is usually virus free) can be cultivated to provide virus free plants.  Plant ‘tissue banks’ can be frozen, then regenerated through tissue culture.  Plant cultures in approved media are easier to export than are soil-grown plants, as they are pathogen free and take up little space (most current plant export is now done in this manner). 5
  • 6. Advantages of micropropagationAdvantages of micropropagation  From one to many propagules rapidly  Multiplication in controlled lab conditions  Continuous propagation year round  Potential for disease-free propagules  Inexpensive per plant once established  Precise crop production scheduling  Reduce stock plant space  Long-term germplasm storage  Production of difficult-to-propagate species DisadvantagesDisadvantagesDisadvantagesDisadvantages  Specialized equipment/facilities required  More technical expertise required  Protocols not optimized for all species  Plants produced may not fit industry standards  Relatively expensive to set up 6
  • 7. 7
  • 8. genetically modified plant breeding does not produce seeds does not respond well to vegetative reproduction8
  • 9. Steps of MicropropagationSteps of Micropropagation 9
  • 11. Steps involved in theSteps involved in the in vitroin vitro MicropropagationMicropropagation Cleaning of glassware Preparation of nutrient medium Selection and sterilization of explant. Inoculation of aseptic explant in to nutrient medium. Proliferation of shoots on a multiplication medium. Transfer of shoots for sub-culturing. Rooting and hardening of plantlets Field trials. 11
  • 12. 12Source : Cadila pharmaceuticals limited
  • 13. Stage 0 : Selection and maintenance of stock plants for culture initiation EXPLANTEXPLANT SHOOT TIPS FROM YOUNG SUCKERSHOOT TIPS FROM YOUNG SUCKER APICAL MERISTEM (1-2 Cm3)APICAL MERISTEM (1-2 Cm3) SURFACED STERILIZATIONSURFACED STERILIZATION 13
  • 14. ExplantExplant Cell, tissue or organ of a plant that is used to start in vitro cultures. 14
  • 15. 15
  • 16. Stage- I : Initiation and establishment of aseptic culture 16 Source: Jain Irrigation Systems Ltd
  • 17. 17
  • 18. 18
  • 20. 20
  • 21. Stage –II : Multiplication of shoot 21
  • 22. 22
  • 24. 24
  • 26.  Healthy/elite plantlets are exposed to the natural conditions in a step wise manner.  It is a gradual acclimatization of in vitro grown plants to in vivo condition.  The plantlets are transferred to the pots/polyghene bag and immediately irrigated with inorganic/nutrient solution.  Plants are kept in the hardening room where controlled conditions of light, humidity and temperature are maintained.  Plants are maintained under high humidity for 10-20 days and subsequently transferred in the field so as to grow under natural conditions. The success rate of micropropagation depends on the survival of the plantlets when transferred from culture to the soil (field). 26
  • 27. Secondary Hardening 27 Plants transferred to nursery bagsPlants transferred to nursery bags Kept for 6 to 8 weeks under 50% shadeKept for 6 to 8 weeks under 50% shade Regular foliar spraysRegular foliar sprays Variation if observed is discardedVariation if observed is discarded Plant ready for sale (1 feet height)Plant ready for sale (1 feet height)
  • 28. 28
  • 29. 29
  • 30. Factors Affecting micro-propagation 1. Growth Media 2. Environmental Factors 3. Explants Source 4. Genetics 30
  • 31. Method of Micro-propagationMethod of Micro-propagation 31
  • 32. Method of Micro-propagation A. Meristem tip cultureA. Meristem tip culture B. Callus cultureB. Callus culture C. Suspension cultureC. Suspension culture E. Protoplast cultureE. Protoplast culture D. Embryo cultureD. Embryo culture F. Ovule cultureF. Ovule culture G. Anther cultureG. Anther culture H. Cell cultureH. Cell culture 32
  • 33. In addition to major use of tissue culture techniques for rapid clonal multiplication of plants, this technique is highly important for several purpose as under: 1. Production and maintenance of pathogen free stock plants; 2. Long term in vitro conservation of germplasm. 3. Selection and regeneration of transgenic plants. 4. Conservation of germplasm. Other Implications of Micro-propagationOther Implications of Micro-propagation 33
  • 34. Micro-propagation Techniques & ProcedureMicro-propagation Techniques & Procedure 34 A. Micro-propagation by NodesA. Micro-propagation by Nodes B. Tip Axillary Bud cultureB. Tip Axillary Bud culture C. Micro-cross Section technologyC. Micro-cross Section technology G. Micro-propagation by Node cuttings in a liquid medium G. Micro-propagation by Node cuttings in a liquid medium D. Shoot tip cultureD. Shoot tip culture E. Seed cultureE. Seed culture F. Pseudo-bulb segment cultureF. Pseudo-bulb segment culture
  • 35. 35 1. Sterilize Petri dishes and prepare the laminar flow chamber by disinfecting the internal surfaces with alcohol. Sterilize the tools with an instrument sterilizer and place them on a sterile dish. 2. Open the tube, take off the plantlet and place it on a Petri dish with the help of forceps. 3. Remove the leaves and cut the nodes. 4. Open a tube containing fresh sterile medium and place a node inside, trying to plunge it slightly into the medium with the bud up. Close the tube. 5. Seal the tube with a gas-permeable plastic tape and label it correctly. It is recommended to place two explants in 16 x 125 mm tubes, three in 18 x 150 mm tubes, five in 25 x 150 mm tubes, and 20-30 in magenta vessels. 1. Sterilize Petri dishes and prepare the laminar flow chamber by disinfecting the internal surfaces with alcohol. Sterilize the tools with an instrument sterilizer and place them on a sterile dish. 2. Open the tube, take off the plantlet and place it on a Petri dish with the help of forceps. 3. Remove the leaves and cut the nodes. 4. Open a tube containing fresh sterile medium and place a node inside, trying to plunge it slightly into the medium with the bud up. Close the tube. 5. Seal the tube with a gas-permeable plastic tape and label it correctly. It is recommended to place two explants in 16 x 125 mm tubes, three in 18 x 150 mm tubes, five in 25 x 150 mm tubes, and 20-30 in magenta vessels. procedureprocedure
  • 36. Applications of Tissue Culture 1. Embryo culture 2. Meristem culture 3. Micropropagation 4. Somatic embryogenesis and Organogenesis 5. Somaclonal variation and in vitro selection 6. Anther culture Haploid & Dihaploid Production 7. Protoplast culture (In vitro hybridization – Protoplast Fusion) 8. Germplasm preservation 36
  • 37. Micro-propagation technology developed for some Horticultural Crops in India Sr. No. Crop Institute 1. Annona IIHR, Banglore; BARC, Mumbai; NCL, Pune 2. Banana NCL, Pune; IIHR, Banglore; TNAU, Coimbatore 3. Citrus NBRI, Lucknow; NRC- Citrus, Nagpur 4. Grape IARI, New Delhi 5. Guava GBPUAT, Pantnagar 6. Papaya IARI, New Delhi 7. Pineapple BARC, Mumbai 8. Strawberry TERI, New Delhi 37
  • 38. 38
  • 39. Tissue culture BananaTissue culture Banana 39
  • 41. Tissue culTure pomegranaTeTissue culTure pomegranaTe 41
  • 44. 44 clonal- propagaTion in guavaclonal- propagaTion in guava
  • 45. 45 micro- propagaTion in aonlamicro- propagaTion in aonla
  • 46. 46 micro- propagaTion in Jamunmicro- propagaTion in Jamun
  • 47. 47 micro- propagaTion in bealmicro- propagaTion in beal
  • 48. 48 micro- propagaTion in Figmicro- propagaTion in Fig
  • 49. 49