Solid phase extraction (SPE) is a sample preparation technique used to extract analytes from liquid samples. It offers advantages over liquid-liquid extraction such as being more efficient, quantitative, easy to perform, rapid, and able to be automated with reduced solvent use and time. SPE works by retaining analytes on a solid stationary phase while allowing interfering substances to pass through. Key steps involve conditioning the SPE material, adding the sample, washing away unwanted materials, and then eluting the target analytes. Common SPE materials include reversed phase, normal phase, ion exchange, and adsorbent types which separate compounds based on interactions like hydrophobicity, polarity, and charge.
High-performance liquid chromatography (HPLC), is a technique in analytical chemistry used to separate, identify, and quantify individual components from a mixture.
THE PRESENTATION ABOUT COUNTER CURRENT EXTRACTION GIVINGBDETAIL ON ITS INTRODUCTION, THEORY, EQUATION, EXAMPLES ARE SIMPLIFIED IN EASY WAY TO THE STUDENTS AND READERS LEARNING A TOPIC AT A PLACE ACCORDING TO PCI SYLLABUS FOLLOWED BY CHEMISTRY STUDENTS OF OTHER BRANCHES.
High-performance liquid chromatography (HPLC), is a technique in analytical chemistry used to separate, identify, and quantify individual components from a mixture.
THE PRESENTATION ABOUT COUNTER CURRENT EXTRACTION GIVINGBDETAIL ON ITS INTRODUCTION, THEORY, EQUATION, EXAMPLES ARE SIMPLIFIED IN EASY WAY TO THE STUDENTS AND READERS LEARNING A TOPIC AT A PLACE ACCORDING TO PCI SYLLABUS FOLLOWED BY CHEMISTRY STUDENTS OF OTHER BRANCHES.
Presentation extraction ms. juhi bandreJuhiBandre1
Extraction is defined as a process where one or more solute(s) are removed from one liquid phase (technically called a diluent) by transferring that/those the solute(s) to another liquid phase (or a solvent).
HPLC- high performance liquid chromatographyhirenthakkar4
HPLC- high performance liquid chromatography or high pressure liquid chromatography overall review
good animation & GIF for presentation
detectors in detail
basic instrumentation with detectors
Osmotic drug delivery system by Mr. kailash vilegaveKailash Vilegave
INTRODUCTION
ADVANTAGES OF OSMOTIC DRUG DELIVERY SYSTEM
DISADVANTAGES OF OSMOTIC DRUG DELIVERY SYSTEM
REPORTED CASES REGARDING LIMITATIONS AND ADVERSE EFFECTS OF OSMOTIC DRUG DELIVERY SYSTEM
PRINCIPLE OF OSMOSIS
BASIC COMPONENTS OF OSMOTIC PUMP
HPLC PPT for you If You want to download it then download it .
Its is in original PPT form
Share it & improve your Knowledge.
INTRODUCTION
HPLC is a form of liquid chromatography used to separate compounds that are dissolved in solution.
HPLC instruments consist of a reservoir of mobile phase, a pump, an injector, a separation column, and a detector.
Compounds are separated by injecting a sample mixture into the column. The different component in the mixture pass through the column at differentiates due to differences in their partition behavior between the mobile phase and the stationary phase.
The mobile phase must be degassed to eliminate the formation of air bubbles.
Today’s analytical laboratory is faced with tight deadlines to produce results from testing environmental samples. Too often, solid-phase extraction (SPE) presents a bottleneck in the analytical testing process and may cause poor analyte recoveries and highly variable. Despite advances in analytical instrumentation, sample prep often relies on tedious, manual, and expensive techniques such as liquid-liquid extraction.
Sample preparation of environmental water samples can be automated, however.. Use of automated sample preparation addresses the many challenges that laboratories face when preparing samples and can help improve sample processing turnaround times.
Chromatography presentation goes with this free on-demand webinar. Link to webinar: https://event.on24.com/eventRegistration/EventLobbyServlet?target=registration.jsp&eventid=832348&sessionid=1&key=7401504685427A0804ABBD1F956E617C&partnerrefthermo=undefined&sourcepage=register
Newer technologies have gained popularity and expanded over the last one decade.
Effective separation is crucial in the operation of processes of any industry. A major question is how best can these processes solve the problems and what are the edges which we can push these new technologies. Achievements have been made in (waste) water treatment. Some of the successes are; low cost of operation, high efficiency, less energy consumption and smaller spaces of operation.
Membrane separation processes have been adopted throughout the world. They are
divided based on the size of particles they can let to pass through and the driving force that is used. Talking of pressure driven processes like microfiltration, ultrafiltration and reverse osmosis, they are processes which changed the whole history of water treatment. For example, reverse osmosis has been used in the desalination of brackish water.
Advantages of reverse osmosis in drinking water treatment include: physically removal
of pathogens, effective removal of substrates in the treated water, less biofilm growth, less disinfectant chemical requirement and less disinfection of the byproduct. However, there are some unanswered questions like the exact dosage of the disinfectants we can use and since the disinfectants will be of less amount, how can we compare it to classic technologies? What are the other advantages of using the reverse osmosis?
Presentation extraction ms. juhi bandreJuhiBandre1
Extraction is defined as a process where one or more solute(s) are removed from one liquid phase (technically called a diluent) by transferring that/those the solute(s) to another liquid phase (or a solvent).
HPLC- high performance liquid chromatographyhirenthakkar4
HPLC- high performance liquid chromatography or high pressure liquid chromatography overall review
good animation & GIF for presentation
detectors in detail
basic instrumentation with detectors
Osmotic drug delivery system by Mr. kailash vilegaveKailash Vilegave
INTRODUCTION
ADVANTAGES OF OSMOTIC DRUG DELIVERY SYSTEM
DISADVANTAGES OF OSMOTIC DRUG DELIVERY SYSTEM
REPORTED CASES REGARDING LIMITATIONS AND ADVERSE EFFECTS OF OSMOTIC DRUG DELIVERY SYSTEM
PRINCIPLE OF OSMOSIS
BASIC COMPONENTS OF OSMOTIC PUMP
HPLC PPT for you If You want to download it then download it .
Its is in original PPT form
Share it & improve your Knowledge.
INTRODUCTION
HPLC is a form of liquid chromatography used to separate compounds that are dissolved in solution.
HPLC instruments consist of a reservoir of mobile phase, a pump, an injector, a separation column, and a detector.
Compounds are separated by injecting a sample mixture into the column. The different component in the mixture pass through the column at differentiates due to differences in their partition behavior between the mobile phase and the stationary phase.
The mobile phase must be degassed to eliminate the formation of air bubbles.
Today’s analytical laboratory is faced with tight deadlines to produce results from testing environmental samples. Too often, solid-phase extraction (SPE) presents a bottleneck in the analytical testing process and may cause poor analyte recoveries and highly variable. Despite advances in analytical instrumentation, sample prep often relies on tedious, manual, and expensive techniques such as liquid-liquid extraction.
Sample preparation of environmental water samples can be automated, however.. Use of automated sample preparation addresses the many challenges that laboratories face when preparing samples and can help improve sample processing turnaround times.
Chromatography presentation goes with this free on-demand webinar. Link to webinar: https://event.on24.com/eventRegistration/EventLobbyServlet?target=registration.jsp&eventid=832348&sessionid=1&key=7401504685427A0804ABBD1F956E617C&partnerrefthermo=undefined&sourcepage=register
Newer technologies have gained popularity and expanded over the last one decade.
Effective separation is crucial in the operation of processes of any industry. A major question is how best can these processes solve the problems and what are the edges which we can push these new technologies. Achievements have been made in (waste) water treatment. Some of the successes are; low cost of operation, high efficiency, less energy consumption and smaller spaces of operation.
Membrane separation processes have been adopted throughout the world. They are
divided based on the size of particles they can let to pass through and the driving force that is used. Talking of pressure driven processes like microfiltration, ultrafiltration and reverse osmosis, they are processes which changed the whole history of water treatment. For example, reverse osmosis has been used in the desalination of brackish water.
Advantages of reverse osmosis in drinking water treatment include: physically removal
of pathogens, effective removal of substrates in the treated water, less biofilm growth, less disinfectant chemical requirement and less disinfection of the byproduct. However, there are some unanswered questions like the exact dosage of the disinfectants we can use and since the disinfectants will be of less amount, how can we compare it to classic technologies? What are the other advantages of using the reverse osmosis?
This webinar will provide pesticides residue analysts with valuable information on the development and optimization of chromatographic separations and mass spectrometry methods for the analysis of pesticide residues in food. The expert speakers will share their knowledge in understanding the critical aspects of the method, assisting analysts in optimizing their methods for the most challenging analyses.
Join the experts as they discuss the use of accelerated solvent extraction and QuEChERS techniques for the extraction of pesticide residues from a diverse range of food samples. Tips and tricks for improving the extraction efficiency will be covered, along with selection criteria for each technique by sample type, assisting analysts in modifying existing methods or developing new methods to tackle their analytical challenges
This webinar will provide pesticides residue analysts with valuable information on the development and optimization of gas chromatographic separations and mass spectrometry methods for the analysis of pesticide residues in food. The expert speakers will share their knowledge in understanding the critical points of the method, assisting analysts in modifying existing methods, and understanding instrumental and software technologies with the goal of improving laboratory productivity and reducing the overall cost per sample. The results of experiments for both screening and quantification workflows, using the latest technology, will be presented.
The presentation contains basic introduction to mostly used and versatile reversed phase chromatography, its instrumentation, working and applications. It will be useful for you to understand basic concepts about RP-HPLC.
it is consist osmotic drug delivery system. and its new approaches. its advantage & disadvantage.. principle. etc
and basic camponents and osmotic pump......
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
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These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
2. INTRODUCTION
• SPE is used most often to prepare liquid samples and extract
semivolatile or nonvolatile analytes, but also can be used with
solids that are pre-extracted into solvents.
• SPE products are excellent for sample extraction, concentration,
and cleanup.
• They are available in a wide variety of chemistries, adsorbents,
and sizes. Selecting the most suitable product for each
application and sample is important.
2
3. • Solid phase extraction (SPE) is an increasingly useful sample
preparation technique.
• With SPE, many of the problems associated with liquid/liquid
extraction can be prevented, such as incomplete phase
separations, less-than-quantitative recoveries, use of expensive,
breakable specialty glassware, and disposal of large quantities of
organic solvents.
• SPE is more efficient than liquid/liquid extraction,yields
quantitative extractions that are easy to perform, is rapid, and can
be automated. Solvent use and lab time are reduced.
3
4. USES
• Removal of interferences and column killers
• Concentration or trace enrichment of the analyte
• Desalting
• Solvent exchange
• In situ derivatization
• Sample storage and transport
4
6. Reversed Phase
Reversed phase separations involve a polar (usually aqueous; or
moderately polar sample matrix (mobile phase) and a non-polar
stationary phase.
Several SPE materials, such as the alkyl- or aryl-bonded silicas (LC-
18, ENVI-18, LC-8, ENVI-8, LC-4) are in the reversed phase
category)
Here, the hydrophilic silanol groups at the surface of the raw silica
packing (typically 60Å pore size, 40μm particle size) have been
chemically modified with hydrophobic alkyl or aryl functional
groups by reaction with the corresponding silanes
6
8. • Retention of organic analytes from polar solutions (e.g. water)
onto these SPE materials is due primarily to the attractive forces
between the carbon-hydrogen bonds in the analyte and then
functional groups on the silica surface.
• These nonpolar-nonpolar attractive forces are commonly called
van der Waals forces, or dispersion forces.
• To elute an adsorbed compound from a reversed phase SPE tube
or disk, use a nonpolar solvent to disrupt the forces that bind the
compound to the packing.
8
9. • LC-18 andLC-8 are standard, monomerically bonded silicas.
Polymerically bonded materials, such as ENVI-18 and ENVI-8,
result in a more complete coverage of the silica surface and
higher carbon loading.
• Polymeric bonding is more resistant to pH extremes, and thus is
more suitable for environmental applications for trapping
organic compounds from acidified aqueous samples.
9
10. Normal phase
• Normal phase SPE procedures typically involve a polar analyte, a
mid- to nonpolar matrix (e.g. acetone, chlorinated solvents, and
hexane), and a polar stationary phase.
• Polar-functionalized bonded silicas (e.g. LC-CN, LC-NH2, and LC-
Diol), and polar adsorption media (LC-Si, LC-Florisil, ENVI-
Florisil, and LC-Alumina) typically are used under normal phase
conditions.
• Retention of an analyte under normal phase conditions is primarily
due to interactions between polar functional groups of the analyte and
polar groups on the sorbent surface.
10
11. • These include hydrogen bonding ,pi-pi interactions, dipole-
dipole interactions, and dipole-induced dipole interactions,
among others.
• A compound adsorbed by these mechanisms is eluted by
passing a solvent that disrupts the binding mechanism —
usually a solvent that is more polar than the sample’s original
matrix
11
12. Ion exchange
• The primary retention mechanism of the compound is based
mainly on the electrostatic attraction of the charged functional
group on the compound to the charged group that is bonded to
the silica surface.
• In order for a compound to retain by ion exchange from an
aqueous solution, the pH of the sample matrix must be one at
which both the compound of interest and the functional group
on the bonded silica are charged.
12
14. Anion Exchange SPE
• The LC-SAX material is comprised of an aliphatic
quaternary amine group that is bonded to the silica surface.
• A quaternary amine is a strong base and exists as a
positively-charged cation that exchanges or attracts anionic
species in the contacting solution — thus the term strong
anion exchanger (SAX).
14
15. • The pKa of a quaternary amine is very high (greater than
14), which makes the bonded functional group charged at
all pH’s when in an aqueous solution.
• As a result, LC-SAX is used to isolate strong anionic
(very low pKa, <1) or weak anionic (moderately low pKa
>2) compounds, as long as the pH of the sample is one at
which the compound of interest is charged.
15
16. Cation Exchange SPE
• The LC-SCX material contains silica with aliphatic sulfonic
acid groups that are bonded to the surface.
• The sulfonic acid group is strongly acidic (pKa <1), and attracts
or exchanges cationic species in a contacting solution – thus the
term strong cation exchanger (SCX).
16
17. • The bonded functional group is charged over the whole pH
range, and therefore can be used to isolate strong cationic (very
high pKa, >14) or weak cationic (moderately high pKa, <12)
compounds, as long as the pH of the solution is one at which
the compound of interest is charged.
• For a cationic (basic) compound of interest, the pH of the
matrix must be 2 pH units below its pKa for it to be charged. In
most cases, the compounds of interest are strong or weak bases
17
19. SPE Is a Five-Step Process
Select the Proper SPE Tube or
Disk
Condition the SPE Tube or Disk
Add the Sample
Wash the Packing
Elute the Compounds of Interest
19
21. • To condition the SPE tube packing, rinse it with up to one tube-
full of solvent before extracting the sample. For disks, use a
volume of 5-10mL
21
22. • Accurately transfer the sample to the tube or reservoir, using a
volumetric pipette or micropipette. The sample must be in a
form that is compatible with SPE.
22
23. • If compounds of interest are retained on the packing, wash off
unwanted,unretained materials using the same solution in which
the sample was dissolved, or another
solution that will not remove the desired
compounds.
• Usually no more than a tube volume of
wash solution is needed, or 5-10mL for
SPE disks.
23
24. • Rinse the packing with a small volume (typically 200μL to 2mL
depending on the tube size, or 5-10mL depending on the disk
size) of a solution that removes compounds of interest, but leaves
behind any impurities not removed in the wash step. Collect the
eluate and further prepare as appropriate
24
25. • SPE tubes can be processed individually using a single tube
processor (Figure A) or with a syringe and an adapter (Figure B).
• The liquid sample is placed in the SPE tube, and the processor or
syringe is used to provide positive pressure to force the liquid
through the tube.
• Positive pressure from an air or nitrogen line also may be used to
force the solutions through the tube.
25
27. • A solution also can be processed through a single SPE tube
using a vacuum flask and rubber stopper.
27
28. • Several SPE tubes can be processed using a centrifuge The solutions
are placed in the SPE tubes and the centrifuge forces the solutions
through the tubes into test tubes.
• Appropriate spin rates must be determined; they can vary depending
on the type and mass of the packing in the tube and the volume of
sample.
28
30. • Choosing the proper SPE device for your application depends
on:
• Sample volume
• Degree of contamination
• Complexity of sample matrix
• Quantity of compounds of interest
• Type and solvent strength of sample matrix
30
31. References
• Guide to solid phase extraction page nos 1-12.
• http://www.sigmaaldrich.com/Graphics/Supelco/objects/4600/
4538.pdf
• http://www.whatman.com/SolidPhaseExtractionSPE.aspx
31