This webinar will provide pesticides residue analysts with valuable information on the development and optimization of chromatographic separations and mass spectrometry methods for the analysis of pesticide residues in food. The expert speakers will share their knowledge in understanding the critical aspects of the method, assisting analysts in optimizing their methods for the most challenging analyses.
Join the experts as they discuss the use of accelerated solvent extraction and QuEChERS techniques for the extraction of pesticide residues from a diverse range of food samples. Tips and tricks for improving the extraction efficiency will be covered, along with selection criteria for each technique by sample type, assisting analysts in modifying existing methods or developing new methods to tackle their analytical challenges
Join the experts as they discuss the use of accelerated solvent extraction and QuEChERS techniques for the extraction of pesticide residues from a diverse range of food samples. Tips and tricks for improving the extraction efficiency will be covered, along with selection criteria for each technique by sample type, assisting analysts in modifying existing methods or developing new methods to tackle their analytical challenges
In this slides contains deep introduction about pesticides and analysis of pesticide residue in vegetables.
Presented by: M. Malarvannan (Department of pharmaceutical analysis),
RIPER, anantapur.
In this slide contains pesticide used in grains, limits as per FSSAI , general detection method for pesticide in Grains and extraction procedures.
Presented by: P.Pavan Kalyan. (Department of pharmaceutical analysis).
RIPER, anantapur.
mass spectrometry for pesticides residue analysis- L1sherif Taha
This is the first lecture in series of lectures on mass spectrometry for pesticides residue analysis. This lecture (1) include Pesticides classification, introduction to mass spectrometry, vacuum system for Agilent GC MS/ MS and AB SCIEX LC MS/ MS
In this slide contains introduction, Pesticide Cycle and Quantification of Organochlorine Pesticides.
Presented by: K.Sandhya Rani. ( Department of pharmaceutical analysis),
RIPER, anantapur.
Elemental analysis does not just start by inserting the sample into the combustion tube! Sample preparation is an essential part of elemental analysis and influences analysis results directly. This webinar addresses all users of elemental analysis and communicates important basics of sample preparation – from homogenization of the sample to wrapping it.
INTip SPE utilizes a patented technology known as Dispersive Pipette XTRaction. This device is unique from all other SPE devices because sorbent is loosely contained within a pipette tip.
This technology enables INTip solid phase extraction for easy sample preparation. The disperser helps to perturb the sample solution and loose sorbent during aspirate and dispense steps. This mixing provides a highly efficient interaction of the sorbent with the analyte of interest resulting in ideal analyte recoveries.
This webinar will provide pesticides residue analysts with valuable information on the development and optimization of gas chromatographic separations and mass spectrometry methods for the analysis of pesticide residues in food. The expert speakers will share their knowledge in understanding the critical points of the method, assisting analysts in modifying existing methods, and understanding instrumental and software technologies with the goal of improving laboratory productivity and reducing the overall cost per sample. The results of experiments for both screening and quantification workflows, using the latest technology, will be presented.
In this slides contains deep introduction about pesticides and analysis of pesticide residue in vegetables.
Presented by: M. Malarvannan (Department of pharmaceutical analysis),
RIPER, anantapur.
In this slide contains pesticide used in grains, limits as per FSSAI , general detection method for pesticide in Grains and extraction procedures.
Presented by: P.Pavan Kalyan. (Department of pharmaceutical analysis).
RIPER, anantapur.
mass spectrometry for pesticides residue analysis- L1sherif Taha
This is the first lecture in series of lectures on mass spectrometry for pesticides residue analysis. This lecture (1) include Pesticides classification, introduction to mass spectrometry, vacuum system for Agilent GC MS/ MS and AB SCIEX LC MS/ MS
In this slide contains introduction, Pesticide Cycle and Quantification of Organochlorine Pesticides.
Presented by: K.Sandhya Rani. ( Department of pharmaceutical analysis),
RIPER, anantapur.
Elemental analysis does not just start by inserting the sample into the combustion tube! Sample preparation is an essential part of elemental analysis and influences analysis results directly. This webinar addresses all users of elemental analysis and communicates important basics of sample preparation – from homogenization of the sample to wrapping it.
INTip SPE utilizes a patented technology known as Dispersive Pipette XTRaction. This device is unique from all other SPE devices because sorbent is loosely contained within a pipette tip.
This technology enables INTip solid phase extraction for easy sample preparation. The disperser helps to perturb the sample solution and loose sorbent during aspirate and dispense steps. This mixing provides a highly efficient interaction of the sorbent with the analyte of interest resulting in ideal analyte recoveries.
This webinar will provide pesticides residue analysts with valuable information on the development and optimization of gas chromatographic separations and mass spectrometry methods for the analysis of pesticide residues in food. The expert speakers will share their knowledge in understanding the critical points of the method, assisting analysts in modifying existing methods, and understanding instrumental and software technologies with the goal of improving laboratory productivity and reducing the overall cost per sample. The results of experiments for both screening and quantification workflows, using the latest technology, will be presented.
The purpose of this course is to develop participant fish identification skills and knowledge of regional freshwater fish species. Participants will learn an overall system for identifying fish. Characteristics of major taxonomic groups within each
family will provide the basis to approach species-level identification. Although emphasis will be placed on the families Cyprinidae, Percidae, Centrarchidae, Catostomidae, and Ictaluridae, specimens from 25 North American freshwater fish
families will be available for study. This course is "hands on" and lab-intensive. Field exercises will provide fresh specimens for identification.
Key Learning Objectives
- Learn how the use of automated software can make SRM development faster and more highly optimized.
- Learn how the use of a compound data store can further simplify method creation.
- Learn how the use of retention time-based SRM acquisition can increase MS/MS sensitivity and make method maintenance easier.
Event Overview:
In recent years, Gas Chromatography-triple quadrupole mass spectrometry has increased in popularity due to its ability to offer lower detection limits in complex matrices, simplified sample prep requirements, and faster analysis times. Of course, new instrument technology presents the need for the acquiring of new skills to harness the advantages offered by its adoption into current workflows.
In this webinar, a strategy for addressing both of these challenges is discussed in the context of new software designed to automate common method development and method maintenance tasks. Also, in addition to making the triple quadrupole easier to use, this strategy can increase sensitivity of the analysis, which will be demonstrated using a complex SRM pesticide method as an example.
For more information: www.thermoscientific.com/tsq8000
Waters analytical technologies enable laboratories to generate more information, complete analyses more rapidly and reduce overall costs throughout key steps in the agrochemical development workflow - including Synthetic Chemistry, Purification, Formulation, Trace Detection, Metabolite ID.
This presentation compares wo methods for the detection of low-level pesticide residues in fruit juice. One involves the use of QuEChERS sample preparation and the other a 'dliute and shoot' approach. Sample preparation is utilised to remove the matrix effects associated with mass spectrometry (MS), using a 'dilute and shoot' approach requires the use of highly sensitive MS detection. It can be seen from the results shown that the 'dilute and shoot' approach can be used in many cases.
LC-MS/MS Solutions for Toxicology and Clinical ResearchSCIEX
La spectrométrie de masse en tandem est devenue un outil essentiel pour les applications cliniques et la recherche biomédicale impliquant l’analyse de biomarqueurs. Contrairement aux méthodes classiques de dosage immunologique, l’analyse par chromatographie liquide couplée à la spectrométrie de masse (LC-MS/MS) permet une analyse très sélective et spécifique de composés multiples en un seul passage, conduisant à une plus grande confiance dans les résultats et permettant de nouvelles découvertes. La LC-MS/MS est idéalement adaptée pour identifier les hormones stéroïdes, la vitamine D et ses métabolites, des peptides ou des protéines et d'autres composés dans des matrices complexes tels que le sang, l'urine, la salive et les lysats céllulaires. Aujourd'hui, la LC-MS/MS est également la méthode préférée de l'analyse médico-légale surpassant les techniques d'analyse traditionnelles - à la fois à des fins de dépistage et de confirmation.
Cette présentation vous présentera comment SCIEX pourrait contribuer à améliorer le monde dans lequel nous vivons en permettant aux scientifiques et aux analystes de laboratoire de trouver des réponses aux défis analytiques complexes auxquels ils sont confrontés.
mass spectrometry for pesticides residue analysis- L2sherif Taha
This is the second lecture in series of lectures on mass spectrometry for pesticides residue analysis. This lecture (2) include: Electron ionization and Chemical ionization
Carbon 14 and archeological ages, Christian and Intelligent Design discussion of source, measurement, results, interpretation, and errors in Carbon-14 dating.
MASS SPECTROMETRY IN THE FIELD OF FOOD INDUSTRYErin Davis
This is a powerpoint presentation solely to give a brief idea about the role of Mass Spectrometry (MS) which is one of the powerful analytical technique.This presentation describes the role of Mass Spectrometry in the field of food industry.These slides deals with the basic principle,working,components,detailed analysis etc.
Mike Riddle has put together a brilliant slideshow examining the truths and misnomers of dating methods currently used by scientists eager to propogate their evolutionary agenda. I hope you enjoy Mr. Riddle's slidshow as much as I have.
mass spectrometry for pesticides residue analysis- L3sherif Taha
This is the third lecture in series of lectures on mass spectrometry for pesticides residue analysis. This lecture (3) include: Electrospray ionization and Atmospheric pressure chemical ionization
Industrial Laboratories around the world are trying to find ways to minimize sample preparation and enhance productivity. The adaptation of modern mass spectrometry instrumentation is desired due to the high sensitivity and selectivity they provide. This presentation will describe how different sample preparation techniques can be simplified and automated for LC/MS/MS analyses.
Implementing a Fully Single-Use, Integrated mAb Biosimilars Purification Plat...MilliporeSigma
Access the interactive recording here: https://bit.ly/2DONZaQ
Webinar summary:
1000L-scale implementation of fully connected, disposable, advanced DSP platform for next generation mAb production.
Within the biopharmaceutical industry, there is a significant shift toward higher productivity processes resulting in improved economics without compromising robustness. Therefore, integrated continuous production technologies are of greatest interest.
Next Generation Biopharmaceutical Downstream Process is a European-funded collaborative project that aims at implementing a fully integrated manufacturing platform for biosimilar mAb based on continuous chromatography, in combination with single-use disposable technologies for all unit operations of DSP on pilot/small production scale together with incorporation of advanced analytical tools.
In this webinar, you will see:
* new DSP purification template producing > 3.3 kg of mAb in 2.5 days in less than 30m²
* proof of concept for the mAb manufacturing of tomorrow
Implementing a Fully Single-Use, Integrated mAb Biosimilars Purification Plat...Merck Life Sciences
Access the interactive recording here: https://bit.ly/2DONZaQ
Webinar summary:
1000L-scale implementation of fully connected, disposable, advanced DSP platform for next generation mAb production.
Within the biopharmaceutical industry, there is a significant shift toward higher productivity processes resulting in improved economics without compromising robustness. Therefore, integrated continuous production technologies are of greatest interest.
Next Generation Biopharmaceutical Downstream Process is a European-funded collaborative project that aims at implementing a fully integrated manufacturing platform for biosimilar mAb based on continuous chromatography, in combination with single-use disposable technologies for all unit operations of DSP on pilot/small production scale together with incorporation of advanced analytical tools.
In this webinar, you will see:
* new DSP purification template producing > 3.3 kg of mAb in 2.5 days in less than 30m²
* proof of concept for the mAb manufacturing of tomorrow
Automated sample preparation using the GERSTEL MPS Series and MAESTRO softwar...GERSTEL
During This Free Presentation, You Will Learn:
How the GERSTEL MPS and MAESTRO software can be used to automate a variety of sample preparation techniques as well as injection of samples.
Specific applications that use automated sample preparation prior to injection into an LCMSMS system.
The wide variety of automated sample preparation options offered by GERSTEL.
Automated Dried Blood Spot extraction and analysis using LC/MS/MS.
Automated Liquid-Liquid extraction and analysis by LC/MS/MS of vitamin B compounds from fortified drinks.
Automated SPE of drugs of abuse from oral fluid samples and analysis by LC/MS/MS.
Enhancing Sensitivities and Peak Capacities for UHPLC-MS Fast Gradient Analys...Sandy Simmons
When compared to 1.7 μm fully porous materials, the ultra-high
efficiency and low backpressures provided by Kinetex core-shell
2.6 μm columns, provides users opportunities to go beyond what
is traditionally accepted for UHPLC runs
Determination of Common Counterions and Impurity Anions in Pharmaceuticals Using a Capillary HPIC System with Suppressed Conductivity and Charge Detection
Recently, identification and quantification of ions in early stage drug development has gained increasing attention, because the APIs maybe contaminated with different counter ions from synthesis steps, and because selecting the counter ion to enhance APIs’ solubility and stability is becoming a key step in formulation development. This presentation demonstrates the identification and quantification of 22 commonly found anions in pharmaceuticals in a single run using a high-pressure capillary IC system (HPIC) with 4-μm particle ion –exchange column, and CD-QD dual detectors.
Using LC-MS/MS and Advanced Software Tools to Screen for unknown and Non-targ...AB SCIEX India
LC-MS/MS is a powerful tool for the analysis of Pharmaceuticals and Personal Care Products in environmental samples. The combination of high resolution LC separation and high sensitivity MS/MS is the most powerful tool to screen and quantify targeted compounds.
Spps and its applications for bioactive peptides(Rajendra Sonawane)Rajendra Sonawane
Bioactive peptides are protein fragments which have a positive impact on the functions and conditions of
living beings. Peptides have shown several useful properties for human health, including antimicrobial, antifungal, antiviral,
and antitumor activities.
High-throughput capillary-flow LC-MS proteomics with maximum MS utilisationAlexander Boichenko
The slide deck that describes a set of high-throughput low-flow LCMS applications and setups using Thermo Scientific UltiMate 3000 RSLCnano system, Q Exactive HF-X mass spectrometer, and EASY-Spray or linear Acclaim PepMap columns. We explain the improvements of ESI MS signal while the flow rate is reduced and address the most important topics for nano- and capillary-flow LCMS applications: robustness, sensitivity, throughput. Additionally to optimizing methods for standard pre-concentration (onto-trap) injection setup we also showcase a novel tandem capillary-flow LCMS setup the is easy-to-use and allows to achieve near 100% MS utilization of MS time.
Modern liquid chromatography hardware and software embrace larger parts of our laboratory workflows than ever before. From sample preparation to sample vial labeling, from setting-up Liquid Chromatography runs to instant result calculation – everywhere along the workflow software and hardware automate work steps which have required manual action before. Next to better productivity, the automation and improved technologies also result in enhanced quality and result consistency.
The seminar reviews very practical examples which all users can relate too. It covers an attractive variety of application areas and analytical challenges.
Similar to Pesticide Residue Analysis Webinar Series: Tips and Tricks for the Whole Workflow (20)
This webinar will provide pesticides residue analysts with valuable information on software method development and data processing for the analysis of pesticide residues in food for both LC–MS and GC–MS. Technical experts will review the latest in software advances to help with data interpretation and reporting.
This presentation will focus on the new USP Chapter <2232> on elemental contaminants in dietary supplements. In particular, it will discuss the permitted daily exposure (PDE) limits of the four heavy metals of toxicological concern defined in the chapter and the different options for measurement strategies to meet these limits. In addition it will give an overview of the new USP Chapter <233>, which describes the suggested sample preparation, instrumental techniques and validation protocols required to demonstrate compliance of the analytical procedure used.
In this webinar Dr. Bertrand Rochat of Faculté de Biologie et de Médecine of the Centre Hospitalier Universitraire Vaudois (CHUV) at Lausanne discusses the paradigm shift to high resolution mass spectrometry (HRMS) in clinical research for quantitative analyses (sensitivity, selectivity, etc.). Quantifications in high resolution full scan or MS/MS mode will be compared with triple quadrupole MS. He will present Quan/Qual analysis with a study on the fate of an anti-cancer agent in human: with over 40 metabolites being identified and quantified; as well as metabolomics data underscoring the versatility of high resolution Orbitrap MS.
Many factors impacting the measurement precision of ICP-OES and ICP-MS are still often neglected for everyday operation, however. Sample preparation is one of the factors that play a crucial role in the success of high-quality sample analysis. In this webinar, our experts will discuss sample preparation to: 1) improve analysis precision 2) make difficult samples easy to be analyzed 3) eliminate sample dilution to minimize error introduction.
For more information, please visit here: http://chrom.ms/CtRtKpw
The webinar is all about Ultra High Pressure Liquid Chromatography (UHPLC) performance and how new column technology can deliver the best separation power and be married with the best UHPLC system to ensure an outstanding result. It covers how chromatographers can ensure that even very complex and unfamiliar samples are assayed with the highest scrutiny possible? The webinar discusses how to get the most out of solid core column technology with the right UHPLC system. It covers the use of an extremely long column approach for ultra-high resolution assays and the outlines the importance of robustness and retention time stability.
In the pharmaceutical arena there is great interest in solid core technology, where there is a broad range of sample types as well as requirements throughout the process of developing new chemical entities. The presentation looks at how solid core technology can be readily adapted to cope with the challenges associated with the pharmaceutical sector, looking at various sample matrices and molecular entities, from small molecules to large biomolecules. The presentation gives an insight into how varying the solid core to porous layer allows the user to optimize separation performance by reducing extra band broadening. Data presented demonstrates how this technology is more robust than fully porous systems when analyzing biological extracts, routinely used in DMPK departments, resulting in longer column lifetimes.
Stationary Phase and Mobile Phase Selection for Liquid Chromatography
The presentation focuses on how to choose the appropriate mode of separation, the correct column and highlights the importance of the correct mobile phase. This approach will be applied to a wide selection of compound types ranging from proteins, peptides, glycans to small pharmaceutical molecules and their metabolites. It will also look at specific application areas for monoclonal antibody analysis, namely: titer, aggregation, charge and oxidation variant. Platform methods for biologics characterization are also discussed.
Investigation into the design and application of solid core stationary phases has led to a better understanding of how the phases work and has resulted in their design aligned to the structure of the analytes being separated. The current range of columns available is discussed both in terms of selectivities, and also morphologies, allowing informed decisions to be made by the chromatographer. Using real life examples, coupled with advanced modeling, the effects of the particle size and morphology will be given for both small and large molecules, offering an insight into what the future holds for solid core products.
Over the past decade, the number of mAb candidates entering the clinical pipeline has grown significantly. In addition, the number of ADCs that use mAb specificity to carry drug payloads to target sites has increased. As a result, analytical characterization is in high demand.
This webinar discusses new innovations in sample preparation, column technology, UHPLC, and high resolution mass spectroscopy (HRMS) that allow the development of analytical methods with run times of less than 5 minutes for all routine methods.
Over the past decade, there have been a growing number of mAb candidates entering the clinical pipeline. This results in a large increase on the demand for analytical characterization. This seminar discusses advances in analytical method development with analytical run times below 10 minutes for all routine methods with intelligent, integrated chromatography workflows. Orbitrap technology has been established as the most powerful MS technology for protein characterization. How this can be incorporated into a complete workflow for bio-pharma analysis is also discussed.
Overview of webinar:
Rechargeable, manganese-based, lithium-ion batteries (LiBs) are environmentally friendly, have a good safety record, and can be made at a lower cost than other metal-based LiBs. However, they have a shorter lifetime. Much research has been spent on improving product safety, cycle life, and product performance, yet understanding fundamental processes and degradation mechanism in LiBs remains a challenge. Identifying breakdown products and understanding degradation processes can lead to enhancing battery performance, improvements in product safety, and insight into component failure mechanisms.
Analysis of Disinfection Byproducts by Ion Chromatography
In this presentation, the use of ion chromatography for the determination of bromate, chlorate and haloacetic acids for compliance monitoring according to various ISO standards (15061, 11206, 10304-4, 23631) and U.S. EPA Method 557 will be discussed. Examples will include IC methods using electrolytically generated hydroxide eluents on an RFIC™ system.
Analysis of Anions and Cations in Produced Water from Hydraulic Fracturing Using Ion Chromatography
This presentation describes the use of ion chromatography (IC) to determine anions and cations in produced water from three different hydraulic fracturing sites. Considerable variation in ion concentration was found, which was attributed to differences in the geology of the locations from which samples were obtained.
Analysis of Cations in Hydraulic Fracturing Flowback Water from the Marcellus Shale Using Ion Chromatography
This presentation describes the determination of cations in hydraulic fracturing flowback water using ion chromatography. In this work, sodium was most abundant, followed by calcium, strontium, magnesium, potassium, barium, ammonium, and then lithium, respectively. The quantity of scale-forming ions, such as calcium, strontium, and barium, is particularly informative because it can be used to determine the amount of anti-scaling agent in fracturing fluid mix that will maximize hydrocarbon recovery.
Determination of Carbohydrates in Various Matrices by Capillary High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD)
This presentation describes the combined advantages of a reagent-free capillary format Ion Chromatography (IC) to determine monosaccharides and disaccharides in various applications, from low concentrations in synthetic urine samples to high concentrations in beverage samples. In a reagent-free IC system, the hydroxide eluent is electrolytically generated inline to deliver accurate and precise concentrations for isocratic or gradient separations by only adding deionized water. Eluent generation eliminates carbonate contamination and errors from manual preparation. A capillary scale system with µL/min flow rates can run 24/7, always on and always ready for samples.
High-performance anion-exchange chromatography with pulsed amperometric detection is valuable for oligosaccharide analysis with the value derived from the high-resolution separation followed by sensitive detection of native oligosaccharides. In this presentation the application of HPAE-PAD to oligosaccharides released from glycoproteins is demonstrated.
Today’s analytical laboratory is faced with tight deadlines to produce results from testing environmental samples. Too often, solid-phase extraction (SPE) presents a bottleneck in the analytical testing process and may cause poor analyte recoveries and highly variable. Despite advances in analytical instrumentation, sample prep often relies on tedious, manual, and expensive techniques such as liquid-liquid extraction.
Sample preparation of environmental water samples can be automated, however.. Use of automated sample preparation addresses the many challenges that laboratories face when preparing samples and can help improve sample processing turnaround times.
Chromatography presentation goes with this free on-demand webinar. Link to webinar: https://event.on24.com/eventRegistration/EventLobbyServlet?target=registration.jsp&eventid=832348&sessionid=1&key=7401504685427A0804ABBD1F956E617C&partnerrefthermo=undefined&sourcepage=register
Total workflow solutions that cater every budget, performance or throughput requirement for confirmatory dioxin analysis were discussed in the Thermo Scientific Lunch Seminar at the Dioxin 2014 conference. D. Hope, CEO & Owner Pacific Rim Laboratoris, presented about the economies of POPs analysis from the point of view of a leading laboratory using the very latest dioxin method kits. C. Cojocariu, Thermo Fisher Scientific, discussed recent changes in EU regulations which bring new opportunities for more labs to participate in dioxin analysis and about validating methods using Gas Chromatography triple quadrupole for PCDD/Fs with reference to the new EU Commission Regulation No. 709/2014.
More from Chromatography & Mass Spectrometry Solutions (20)
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Pesticide Residue Analysis Webinar Series: Tips and Tricks for the Whole Workflow
1. Pesticide Residue Analysis Webinar
Series PART TWO: Workflow Guide
for the Use of LCMS
Richard Fussell
Vertical Marketing Manager,
Food and Beverage
Claudia Martins
Applications Program Manager,
Environmental and Food Safety
2. 2
Webinar Series Objectives
• To provide pesticide residue analysts with valuable
information on development and optimization of method
workflows for the analysis of pesticide residues in food.
• To understand the challenges, limitations and advantages of
multi-residue workflows for pesticides residues in food
• To review the latest developments in:
• sample preparation
• targeted and screening techniques using GC-MS/MS and LC-MS/MS
• software advances to help with data interpretation and reporting.
3. 3
1. Sample Prep: March 24th 2. LC-MS Analysis: April 29th
3. GC-MS Analysis: June 17th 4. Data Processing/Analysis: July 15th
Typical Pesticides Workflow
Register at www.chromatographyonline.com/LCGCwebseminars
4. 4
Thermo ScientificTM TSQ
QuantivaTM and TSQ EnduraTM
Next generation Triple Quadrupole
MS/MS
LC-MS Timeline: Developments and Impact
Electrospray QQQ-MS
1995
2015
APCI, Single quadrupole MS
Karen A Barnes et al, Rapid Comunications in
Mass Spectrometry (1995) 9, 1441-1445.
Christel L. Hetherton et al, Rapid
Communications in Mass
Spectrometry, 2004, 18, 2443-2450.
Quantification Limits
QuEChERS
2002
-‐
2011
Total #
Analytical
Results (x1000)
Avg # pesticides
analyzed per
sample
Total # residues
detected (x10)
# of different
residues
detected
Thermo ScientificTM
Q ExactiveTM Focus
Routine HRAM MS/MS
2013
2000
2005
2010
5. 5
Pesticide Residues Analysis Laboratory
Sample &
Analyte
Variability
Number of
Analytes
High
Throughput
Cost
Reduction
Screening
Identification
Quantitation
LC-MS
&
GC-MS
Sensitivity
6. 6
Different Steps in Analytical Methodology
Sample
Preparation
Chromatographic
Separation
Detection
Data
Processing
&
Reporting
7. Original QuEChERS Protocol*
Weight 10 g of sample
* Anastassiades et al. J AOAC Int., 86 (2) 412-431
Analysis – GC-MS and LC-MS
Shake Vigorously 1 min
Shake Vigorously 1 min
Shake 30s + Centrifuge
Shake vigorously 30s + Centrifuge
Add 10 mL Acetonitrile
Add ISTD - Solution
Add 4 g MgSO4 + 1 g NaCl
Take Aliquot and Mix with MgSO4 and PSA
8. Pros & Cons – Most-used Sample Prep Techniques
QuEChERS + Standard Method
+ Effective
+ Simple
- Contains manual
steps
- Difficulties when
dealing with high fat
content and
carotinoides or
chlorophyll rich
samples
Dilute & Shoot
(Liquid Samples)
+ Simple
- Dilution needed
- Highly Sensitive
and Robust
methods needed
- Regular
maintenance of
instrumentation
needed
Turbulent Flow
Chromatography
+ Automated
- Needs upgrade of standard configuration
- Setup can be time-consuming
OFFLINE
ONLINE
9. 9
9
How Does Thermo Scientific TurboFlow Technology
Work?
• Big particle size + high flow rate turbulent flow
• Small molecules diffuse into pores faster than large
molecules
• Components of interest (analytes) are retained
• Matrix components are flushed through
• A change in mobile phase composition
elutes analytes of interest to the column (or
an analytical detector)
small moleculescolumn pores
analytes
analytes
10. 10
10
Examples of the Use of Turbulent Flow Chromatography
Analytes
Application Field
Sample
TFC column
Loading Flow Rate
Injection Volume
Detection
Sensitivity
PFOS
Environmental Analysis
River Water
50 x 1.0 mm, 50µm C18
1 mL/min
1mL
APPI-MS
5.35 ng/L (LOD)
Anti-Infectives
Environmental Analysis
Waste Water
50 x 1.0 mm, 50µm C18 XL
3 mL/min
1mL
ESI-MS/MS
15-53 ng/L (LOD)
Enrofloxacin
Ciprofloxacin
Food Analysis
Edible Tissues
50 x 1.0 mm, 50µm C18
Cyclone
5 mL/min
20µL
ESI-MS/MS
25 µg/Kg (LOQ)
Quinolones
Food Analysis
Honey
50 x 0.5 mm, 60µm Cyclone
1.5 mL/min
160µL
ESI-MS/MS
5 µg/Kg (MLOQ)
Veterinary drugs
Food Analysis
Milk
50 x 0.5 mm, 60µm Cyclone –
Cyclone P (connected in
tandem)
1.5 mL/min
50µL
ESI-MS/MS
0.1-5.2 µg/L
O.Núñez et al. J Chrom A
2012, 1228, 298-323.
14. 0
0
50
100
Polar Pesticides in Food Extracts
Acephate in acetonitrile
Acephate in acetonitrile-H2O
Polar pesticides – dilution of QuEChERS extract necessary before injection
to achieve good peak shape
Alternatives:
• Reduce injection volume
• Increase tubing diameter
between injector and column
15. Time
(min)
% H2O 5mM Ammonium Formate
0.1% FA
% MeOH 5 mM Ammonium Formate
0.1% FA
0.0 98 2
0.5 98 2
2.0 60 40
20.0 5 95
22.0 5 95
22.1 98 2
25.0 98 2
Thermo Scientific™ Accucore™ aQ
(100 x 2.1 mm, 2.6 µm)
Chromatographic Separation of 440 Pesticides
19. 19
19
• Recoveries close to 100%
most of compounds in range 80-110%
• Small initial sample size
<1 g homogenised sample
• Multiresidual assay
48 pesticides of different classes - extendable
• Minimal Matrix Effects
• High Sensitivity
all (except chlormequate) fullfills MRL criteria –below 10 ug/kg
• Good Intermediate Precision
below 25%
• High speed
total run time 13 min/sample (excl. extraction time)
Method Validation Results
Laszlo Hollosi et al. Chromatographia, 2012, 75:1377-1393.
21. 21
What Workflow Should I Follow?
SAMPLE
Target Analysis Non-target Analysis
Identification
Screening
Profiling
Fingerprinting
Differential Analysis
Database & Spectrum
Libraries
Identification
Quantitation
22. 22
The Industry’s Leading Portfolio of MS Solutions
HRAM
MS, MSn
Applied
Markets
Research
Markets
Non-targeted
Analysis
Targeted
Analysis
Quantitative Qualitative
• Biomarker Discovery
• Proteomics
• Metabolism
• Metabolomics
• Proteomics
• Bioanalysis
• Food Safety
• Environmental
• Clinical/Toxicology
• Metabolomics
• PTM Analysis
• Lipidomics
Transform
Your Science
Ion TrapsTriple Quads
Tribrid OrbitrapExactives
23. 23
Thermo Scientific TSQ Quantiva and Thermo
Scientific TSQ Endura
Thermo Scientific™ TSQ Endura™
Triple Quadrupole Mass Spectrometer
Thermo Scientific™ TSQ
Quantiva™ Triple Quadrupole
Mass Spectrometer
TSQ Quantiva TSQ Endura
Mass Range 10-1850 10-3400
Max SRM Number 30,000 SRMs 30,000 SRMs
SRM/Sec 500 SRMs/sec 500 SRMs/sec
Ion Optics Active Ion
Management (AIM)
• Ion Max NG source
• Electrodynamic
ion funnel
• ion beam guide with
neutral blocker
• 6 mm HyperQuad
quadrupoles with
asymmetric RF drive
S-LENS with Beam
Blocker Technology
Quadrupole Design 4mm Quadrupoles
with Asymetric RF
Reserpine
Specification (RFQ)
100,000 : 1 S/N for
1 pg Reserpine
10,000 : 1 S/N for
1 pg Reserpine
Extreme Quantitative Value
• Designed for non-stop operation.
• For scientists who need to run
routine samples day in and day out.
Extreme Quantitative
Performance
• Designed for the most
challenging assays.
• For scientists needing to stay at the
forefront of analytical technology.
24. 24
What’s the Alternative to Optimization by Infusion?
Select compounds from Thermo Scientific™
TraceFinderTM Compound Database to create
instrument method
Select Pre-configured TSQ method with
LC and SRM conditions included - Load and Go!
Edit existing pre-configured method to create new
OR
OR
25. 25
Typical Setup of a LC-QqQ-MS/MS Method
1. Compound Database 2. Master method
4. Data Review & Report 3. Acquisition
29. 29
Advantages of H-SRM in the Analysis of Pesticides
O.Núñez et al. J Chrom A ., 2012, 1249, 164-180.
m/z
• Remove background
interferences
• Smooth baselines
• Improve % CV
• Increase signal-to-noise
30. 30
T-SRM: Pictorial Representation
0 5 10 15 20 25 30 35 40 45 50 55
Time (min)
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
RelativeAbundance
29.85
15.15 29.68
27.16 39.01
34.40
26.28 40.72 51.6734.02
32.99
48.243.99 42.9315.53 36.34
30.18
7.93
55.3922.97
20.40 49.616.44 14.5310.61 47.98 52.102.83
NL:
3.59E6
TIC MS
• Instead of continually monitoring all transitions across the entire
chromatographic window, SRMs can be monitored in distinct time windows
(based on the retention time of the analyte).
• Increases number of transitions that can be monitored.
• Increases dwell time for each transition monitored.
• Therefore improved %CV.
32. 32
The Use of QqQ for Pesticide Residue Analysis
• Compatibility with simple sample preparation: QuEChERS, extract-n-shoot
• Compatibility with UHPLC
• High Sensitivity
BUT…
• Targeted analysis only
• Limited screening capability of MS/MS (~ 200 compounds)
• Data evaluation is time consuming
• Multiple instruments to cover whole scope of method
34. 34
Skip the Matrix: Dummy Transitions Concentrations
“…the selectivity of LC-HRMS exceeds LC-MS/MS, if
high resolution mass spectrometry (HRMS) data is
recorded with a resolution of 50,000 full width at half
maximum (FWHM) and a corresponding mass
window…”
Kaufmann et al: Anal. Chim. Acta., 2010, 673, 60-72.
35. 35 COMPANY CONFIDENTIAL
Q Exactive &
Q Exactive Plus
• Orbitrap analyzer
• Mass Range m/z 50 - 6000
• Mass Accuracy <1ppm
• Max. Mass Resolution
>140,000
• Scan speed up to 12Hz
MS and MS/MS
• Spectral Multiplexing
Q Exactive HF
• Ultra High Field Orbitrap
analyzer
• Mass Range m/z 50 - 6000
• Mass Accuracy <1ppm
• Max. Mass Resolution
>240,000
• Scan speed up to 18Hz MS
and MS/MS
Exactive Plus (EMR)
• Orbitrap analyzer
• Mass Range m/z 50 - 6000
• Mass Range EMR: 300 -
20,000
• Mass Accuracy: <1ppm
• Mass Resolution >140,000
• Scan Speed up to 12 Hz
Q Exactive Focus
• Orbitrap analyzer
• Mass Range m/z 50 - 2000
• Mass Accuracy <1ppm
• Max. Mass Resolution
>70,000
• Scan speed up to 12Hz MS
and 12 Hz MS/MS
• Polarity switching
• Top 2 ddMS2
• No Multiplexing
• Refined workflows that are
optimal for routine
laboratories
VALUE
PERFORMANCE
What’s the Alternative to LC-QqQ-MS/MS?
37. 37
Pesticides in Fruits and Vegetables by LC-HRMS
• QuEChERS
• 5 X Dilution
Sample
Treatment
• Accucore aQ (13min)
• FS/ddMS2 (Inclusion List)
LC-HRMS
Data presented at EPRW 2014
40. 40
What Scan Mode is Right for Your Workflow?
PRM/T-MS2
Full Scan MS
Matrix
Leek extract spiked with 0.1 ppb imazalil
Precursorion@R=70,000Production@R=70,000
3.275 ppm
Product Ion
M.M.Gomez-Ramos et al. J Chrom A., 2013, 1287, 24-37.
41. 41
SANCO/12571/2013
• Method Validation & Quality Control Procedures for Pesticide Residues
Analysis in Food & Feed
• Implemented 1/1/2014 HRAM criteria
≥ 2 ions (pref. including quasi molecular ion)
< 5 ppm mass accuracy
At least one fragment ion
Resolution typically >20000FWHM
42. 42
EU 2002/657/
EC
SANCO
12571/2013
EU-RL-
MB SOP
Gerssen
(2010)
Mol
(2012)
Domènech
(2014)
Kumar
(2014)
Pitarch
(2007)
Analytes
-
Pesticides
Lipophilic
Toxins
Lipophilic
Toxins
Pesticides
Lipophilic Toxins
Ronidazole
Nitroimidazoles
Priority organic
micropollutants
Matrix
Food
Food and
Feed
Molluscs
Shellfish
Vegetables &Fruits
Mussels
Muscle
Water
Technique
HRMS
HRMS
LC-MS/MS
LC-MS/MS
LC-HRMS/MS
LC-HRMS/MS
LC-HRMS/MS
GC-MS/MS
Mass Accuracy
-
˂ 5ppm
-
-
˂ 5ppm
˂ 5ppm
˂ 5ppm
-
Mass Resolving
Power (FWHM)
-
≥20,000
-
-
≥20,000
≥20,000
≥70,000
-
Retention Time
(RT) Tolerance
2.5%
2.5%
Not exceed
3%
5%
1%
Mean ±3SD (not
relative to time)
±1%
Agreement - RT
samples &
standards
Diagnostic Ions
≥ 2
≥ 2
1 precursor
1 precursor
≥ 2
1
2
1 or 2 precursors
Fragment Ions
-
At least one
At least 2
precursor-
product
transitions
2 products
At least one
1
At least 1
>20,000FWHM
At least 2
precursor-product
transitions
Isotope Ions
-
-
-
-
M+1
M+2
M+1
-
-
Ion Ratio
Relative
intensity (% at
base peak)
Relative
intensity (%
at base peak)
Must be
recorded
As described
2002/657/EC
Fragment Ion Ratio:
Diagnostic/Fragment
Isotope Ion Ratio:
Diagnostic/M+1 (M
+2)
Fragment Ion
Ratio: Diagnostic/
Fragment
Isotope Ion Ratio:
Diagnostic/M+1
At least one
Ratio between
quantitative and
confirmation
transition
Fragment-Isotope
Ion Ratio Tolerance
2 IPs for
precursor ion
2.5 IPs for a
product
As described
2002/657/EC
-
As described
2002/657/EC
Independent of
relative intensity
between ions: ±50%
As described
2002/657/EC
-
As described
2002/657/EC
Identification Criteria - Other ExamplesP. Lucci and C.P.B. Martins in Fast Liquid Chromatography – Mass Spectrometry Methods in Food and Environmental
Analysis - World Scientific Publishing Company (March 2015)
43. 43
N. Cortés-Francisco, C. Flores, E. Moyano, J. Caixach. Anal. Bioanal. Chem., 2011 400(10):3595-606.
Importance of Mass Accuracy on Unknown Screening
0
50
100
150
200
250
300
350
0.05 0.1 0.5 1 2 3 4 5 6 7 8 9 10
Mass Accuracy (ppm)
NumberofPossibleCandidates
CHO
CHON
CHONS
CHONSF
m/z 498.9302
44. Trends in pesticide residue analysis
• IC-MS/MS for polar pesticides
• Low flow separations (microflow) for highest sensitivity
• Fast separation (< 15 min/run)
• Matrix effects compensation by dilution (1:100-1000?)
• The use of LC-HRMS as an alternative to LC-MS/MS
• Multi-analyte screening (eg. pesticide & mycotoxin)
45. Conclusions
• It is critical to adapt methodology for maximum performance
• Sample preparation, chromatography, configuration, method set-up, etc
• Different technologies and configurations complement each other to
suit multiple needs
46. 46
Thermo Scientific Food and Environmental
Communities: Resources
• View application notes, on-demand webinars, product information, and
many more resources on our Pesticides and Food Communities Libraries:
www.thermoscientific.com/pesticides www.thermoscientific.com/foodandbeverage