HPTLC- Principle, Instrumentation and Software (Abhishek Gupta)Abhishek Gupta
HPTLC is the improved method of TLC which utilizes the conventional technique of TLC in more optimized way
It is also known as planar chromatography or Flat-bed chromatography.
HPTLC- Principle, Instrumentation and Software (Abhishek Gupta)Abhishek Gupta
HPTLC is the improved method of TLC which utilizes the conventional technique of TLC in more optimized way
It is also known as planar chromatography or Flat-bed chromatography.
The presentation contains basic introduction to mostly used and versatile reversed phase chromatography, its instrumentation, working and applications. It will be useful for you to understand basic concepts about RP-HPLC.
v called as “medium pressure chromatography”
“An air pressure driven hybrid of medium and short column chromatography optimized for rapid separation"
Popularized by Clark Still of Columbia University
An alternative to slow and often inefficient gravity-fed chromatography
The presentation contains basic introduction to mostly used and versatile reversed phase chromatography, its instrumentation, working and applications. It will be useful for you to understand basic concepts about RP-HPLC.
v called as “medium pressure chromatography”
“An air pressure driven hybrid of medium and short column chromatography optimized for rapid separation"
Popularized by Clark Still of Columbia University
An alternative to slow and often inefficient gravity-fed chromatography
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
3. • It is a one step process compared to LLE
• complete removal of interferences
• Disadvantages:
• Variability of SPE cartridges
• Irreversible adsorption of analytes in some
cases
• Poor reproducibility ,because cartridges will
vary from lot to lot while solvents in LLE are
pure so results are reproducible with LLE
5. • Column Killers such as hyd4rophobic
substances, polymeric materials can either
plug or deactivate HPLC column
• If k value greater than 1 then loading large
amount of sample and eluting with stronger
solvent will result in trace enrinchment
increases in detection sensitivity
6. • strong solvent such as ACN or MeOH ELUTES these
analytes from the cartridge in a small concentrated
volume ,which saves evaporation time.
• Used to desalt the samples prior to ion exchange
chromatography
• Conditions of pH and organic phase are selected to
retain the analyte initially, which allows to wash the
inorganic salts from cartridge with water. later
analyte can be washed with organic solvent
7. SPE DEVICES
cartridge, Disk, coated fiber
Cartridges/columns
Solid phase extraction cartridges are available with a
variety of stationary phases, each of which can
separate analytes according to different chemical
properties. Often, the barrel of a medical syringe
made of polypropylene packed with a small amount
of sorbent, usually less than a gram, although
cartridges with up to 10g are commercially
available. The packing is contained in the barrel by
frits, just like an HPLC column.
8. • The outlet of the barrel contains a leur tip so that
needle can be fixed to a direct effluent to a vial or
container
• Frits maintaining particle bed in the cartridge are of
PTFE, polypropylene or stainless steel
• The diameter of the packing material, usually in the
40-micron range, is larger than used in HPLC.
Because efficiency is relatively unimportant in SPE
and cost is more central, irregularly shaped particle
packings rather than spherical packings are used.
• inexpensive
• single time
•
9.
10.
11.
12.
13. • Disks: disks closely resembles membrane
filters
• They are usually flat 1mm in thichness
• Diameter 4mm to 90mm
• Packing :60-90% of total wt
• Sold individually or preloaded
• Packed with pvc or PTFE resins with silica
• Channeling is absent because packing material
embedded in the disc
• Widely used in environment applications
14.
15. • Coated fibers are used for solid phase
microextraction.
• Solid fused silica fiber coated with stationary
phase such as polyacrylate or polydimethoxy
silane
• The fiber dipped in the solution to be analysed
• And the analytes will diffuse in to stationary
phase and get partition in to the coating. the
fiber is removed from the solution and injected
in to the HPLC port.
24. Conditioning of packing
• Methanol –cyano,amino and diol not to be
used for silica gel (methylene chloride)as it is
deactivated by the solvent
• or acetonitrile is passed through the bed
• Purpose : removal of impurities on cartridge
• Solvation of adsorbent
• drying of adsorbent- decreased sample
retention, non reproducible results
25. • After packing is conditioned excess solvent is
removed by blowing air until the solvent drips
from cartridge
• Preconditioning also done by water wash.
• Step 2
• Sample application : sample is dissolved in a weak
solvent added to the cartridge
• Weak solvent allows strong retention of the
sample
• For RP-SPE : weak solvent –water or buffer
• With 10% of organic phase
26. • Application of sample : pipette ,syringe or
pumping
• Step 3: removal of interferences
• Interference removal by using solvent of
intermediate strength
• small amount of organic phase added to
remove hydrophobic substances
• Step 4: elution and collection of the analyte
• Strong solvents are used
