This document summarizes key information about photoreceptors in plants. It describes the major classes of photoreceptors - phytochromes, cryptochromes, and phototropins. Phytochromes detect red and far-red light and regulate processes like seed germination, shade avoidance, and flowering. Cryptochromes detect blue and UV light and mediate responses like inhibition of hypocotyl elongation. Phototropins are blue light receptors that mediate phototropism and chloroplast movement. The document outlines classic genetic studies in Arabidopsis that identified important photoreceptor genes and mutants defective in light responses.
These are the lecture slides, but unfortunately we have to stick only to the external coincidence model and the regulation of transition to flowering- Coupland/Valverde paper. Science 2004
These are the lecture slides, but unfortunately we have to stick only to the external coincidence model and the regulation of transition to flowering- Coupland/Valverde paper. Science 2004
Here is the presentation about Skotomorphogenesis & Photomorphogensis.
A seedling that emerge in darkness is known as skotomorphogenesis which is characterized by etiolation. A seedling that emerge in light is known as photomorphogenesis which is characterized by de-etiolation. effect of duration of photoperiod on floral indication is known as Photoperiodism. Phytochrome present in the chloroplast is responsible for the photoperiodic responses. Phytochromes in chloroplast exist in two interconvertible forms. That is PR and PFR. The sunlight converts PR into PFR that results to initiate transcription of a variety of genes that eventually effect the process of photoperiodism.
intro-hostory and discovery-characteristics of phytochrome-chemical nature of phytochrome-mode of action-mechanism-phytochrome mediated physiological responses-phytochrome is a pigment system:some evidences-role of phytochrome
Meaning of Florigen
Characteristics of Florigen
Mechanism of action
Production of inflorescence meristem
Other functions of Florigen
Mechanism of action during other functions
Pigments which are not directly involved in photosynthesis but have the ability to absorb light and transfer it to the principal photosynthetic pigments are called as Accessory pigments. Phycobilins are water soluble pigments that are fairly joined to a protein, which acts as a accessory pigment in photosynthesis. Phycocyanobilin and Phycoerythrobilin are major phycobilins.
Technique that is used to elucidate mechanism of a reaction or in a metabolic pathway and in a cell. The labeling takes place by exchanging a specific atom with their isotope. The detecting of the isotopes in the product helps to understand the possible mechanism and the stereochemistry in this sequence of the reaction. The detection of the isotopic labels is dependent on the kind of isotope. Radioactive isotopes like 3H 14C are measured radiochemical. Stable isotopes like 2H and 13C are detected for example with NMR- and IR-spectroscopy.
Kristina Melnik & Stephanie Felten (Undergraduate Students)
University of Utah
2014
photoperiodism its discovery,significance,classifications,mechanism,critical day length,quality of light, night break phenomenon,phytochrome.florigen,floering genes, circadian rhythm
Here is the presentation about Skotomorphogenesis & Photomorphogensis.
A seedling that emerge in darkness is known as skotomorphogenesis which is characterized by etiolation. A seedling that emerge in light is known as photomorphogenesis which is characterized by de-etiolation. effect of duration of photoperiod on floral indication is known as Photoperiodism. Phytochrome present in the chloroplast is responsible for the photoperiodic responses. Phytochromes in chloroplast exist in two interconvertible forms. That is PR and PFR. The sunlight converts PR into PFR that results to initiate transcription of a variety of genes that eventually effect the process of photoperiodism.
intro-hostory and discovery-characteristics of phytochrome-chemical nature of phytochrome-mode of action-mechanism-phytochrome mediated physiological responses-phytochrome is a pigment system:some evidences-role of phytochrome
Meaning of Florigen
Characteristics of Florigen
Mechanism of action
Production of inflorescence meristem
Other functions of Florigen
Mechanism of action during other functions
Pigments which are not directly involved in photosynthesis but have the ability to absorb light and transfer it to the principal photosynthetic pigments are called as Accessory pigments. Phycobilins are water soluble pigments that are fairly joined to a protein, which acts as a accessory pigment in photosynthesis. Phycocyanobilin and Phycoerythrobilin are major phycobilins.
Technique that is used to elucidate mechanism of a reaction or in a metabolic pathway and in a cell. The labeling takes place by exchanging a specific atom with their isotope. The detecting of the isotopes in the product helps to understand the possible mechanism and the stereochemistry in this sequence of the reaction. The detection of the isotopic labels is dependent on the kind of isotope. Radioactive isotopes like 3H 14C are measured radiochemical. Stable isotopes like 2H and 13C are detected for example with NMR- and IR-spectroscopy.
Kristina Melnik & Stephanie Felten (Undergraduate Students)
University of Utah
2014
photoperiodism its discovery,significance,classifications,mechanism,critical day length,quality of light, night break phenomenon,phytochrome.florigen,floering genes, circadian rhythm
The 1999 Treaty of Amsterdam states that the European Union is to be an area of freedom, security, and justice. Establishing this freedom within the European Union confronts the EU with the problem of its external borders and the need to safeguard them. Indeed, Europe’s emergent border regime with ever stricter visa policies, tighter border controls, the border agency Frontex and the (partial) externalization of responsibilities to Morocco or Libya have earned the EU the ungainly title “Fortress Europe”.
Yet even though the harmonization of immigration, asylum and refugee policies was an explicit aim of the Amsterdam Treaty already ten years ago, a coherent and legitimate European approach to migration is still wanting, not to speak of the appalling state of the protection of the migrants’ human rights. On the contrary, fragmentation and bilateral agreements are proliferating, the recent agreement between Italy and Libya being a prominent case in point.
The majority of migrants heading towards Europe use regular routes. But with ever increasing legal restrictions also more and more people try to get into Europe irregularly. Especially in the Mediterranean, this has created the phenomenon of the boat people, who try to reach Malta, Lampedusa, and other shores. In the past few years thousands have died trying to reach Europe in their makeshift boats. Others find themselves in desolate camps in Italy or in the Libyan desert. Countless are the cases in which the basic human rights of migrants and refugees are systematically ignored, be it by prohibiting them to apply for asylum, by keeping them in camps indefinitely or by lack of access to health care.
The issues related to migration pose manifold challenges to all affected countries, sending, transit, and regions of destination alike. With the “Global Approach to Migration” (GAM), adopted by the EU in December 2005 at least rhetorically steps have been made towards linking migration and development, as for example in the case of mobility partnerships. This also shows the stronger bargaining position of a number of African states. Structural imbalances –especially Europe’s agricultural policy- persist, though, and it remains to be seen if diplomatic progress will also translate in a deeper respect for the rights of migrants.
The articles in this dossier shed critical light on several related sites of Euro-Mediterranean border management. They look at the “border within” as well as the effects of the exterritorialisation strategy in the Libyan example. They show both the deadly and the “productive” aspects of the border regime. And they analyse the rationale and impacts of such measures as the mobility partnerships between EU and African states or new attempts of cooperation in the Central Mediterranean.
This dossier takes up and pursues central questions discussed at the international conference “Fortress or Area of Freedom? Euro-Mediterranean Border Management” which took place in May 2009 in Berlin.
As summer in Australia is getting longer & hotter in the recent years, it’s extremely important that Australian seniors are well-informed about the consequences the heat can brought upon their well-being, and what they can do to properly cope with hot weather.
This presentation describes in details how photosynthesis works along with its process. It also explains in details on the light-dependent and light-independent reactions.
Cell basic unit of life Biology Chapter No 4Sajjad Mirani
This Slide Contain All Basic Terms and Detail Of Cell With Pictures New Course Of Sindh Text Book Board
By Sajjad Hussain Mirani Sindh University Jamshoro
Chapter 15
The basic unit of life
Characteristics of Life
Macromolecules Needed for Life
Cell Types: Prokaryotic and Eukaryotic
The Microscope
Tour of a Eukaryotic Cell
The Cell Membrane
Transport into and out of Cells
Cell Communication
How Cells Reproduce
How Cells Use Energy
ATP and Chemical Reactions in Cells
Photosynthesis
Cellular Respiration and Fermentation
Learn about a different culture. The culture of risk and love. The culture of caring and teaching. Becoming a missionary is already a selfless way of life. Learn about those willing to do whatever it takes to bring the gospel to those in remote areas.
Los días 20 y 21 de octubre de 2016, la Fundacion Ramón Areces organizó un simposio internacional para analizar las 'Enfermedades raras de la piel: de la clínica al gen y viceversa'. El doctor Fernando Larcher Laguzzi, del CIEMAT-Universidad Carlos III de Madrid-IIS Fundación Jiménez Díaz, ejerció de coordinador.
Models of Human Diseases Conference (2010) Tetrahymena model by Dr. R. Pearl...Medical Education Advising
The Ciliate Protozoan Tetrahymena thermophila as an important animal model organism
Dr. R.E. Pearlman, York University
Models of Human Diseases Conference
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Similar to photomorphogenesis, photosensors, light signal transduction (20)
Consumer-Assisted Selection: Making New Plants that Look, Smell and Taste Be...Kevin Folta
The Plant Innovation Center at the University of Florida is an interdisciplinary group of researchers that span five colleges, conspiring to make fruits and vegetables more attractive. The work uses the model of Consumer Assisted Selection, monitoring consumer preferences and then defining breeding and production priorities based on observed outcomes. The concept is a simple one-- Use what we have learned from analysis of market segments to define what food improvements might look like, then use the best technologies to achieve those goals. (Slides created by Dr. Dave Clark for the PIC, used by Folta with permission).
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How to Change the Hearts and Minds of a Concerned PublicKevin Folta
Slides from 2016 Crop Connect in Winnipeg, Manitoba. The presentation talks about the current problem in the discussion of genetic engineering, and how farm producers need to be part of the solution. presented February 11, 2016
Ethnobotany and Ethnopharmacology:
Ethnobotany in herbal drug evaluation,
Impact of Ethnobotany in traditional medicine,
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The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
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This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
How to Split Bills in the Odoo 17 POS ModuleCeline George
Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
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Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
3. -synthesized as Pr in darkness
-converted by red light (max=
666 nm) to Pfr
-Pfr is biologically active form
-Far-red light (730 nm) converts
to Pr
4. Early Developmental Effects of Phytochromes
Phytochromes regulate (100’s of processes
described)
-- stem elongation
-- cotyledon expansion
-- chloroplast development (greening)
-- apical hook opening
-- gene expression
Microarray analyses show that the early
effects of phytochromes are to induce the
expression of transcription factors that then
alter the expression of genes involved in
photomorphogenic development
(Tepperman et al., 2001).
5. Shade avoidance responses
--- Induced under FR enriched circumstances
---EOD-FR(End of Day Far Red) can induce a
similar response
---Can be induced by shade or light reflected from
neighboring plants (“density sensor?”)
---Controlled by Type II phytochromes
---Plants possess long petioles, longer stems and
unusually elongated organs in general.
Regulation of Flowering time
---phyB mutant shows an early flowering in LD (long day 16H/8D) condition.
---phyA mutant shows an late flowering in Extended SD (8L/8 FR-enrich/8D) condition.
Long-term Roles for Phytochrome in Regulation of Plant Physiology
6. ENTER ARABIDOPSIS
Koornneef M., Rolff E. and Spruit C.J.P. (1980) Genetic control of light-
inhibited hypocotyl elongation in Arabidopsis thaliana (L.) Heynh. Z.
Pflanzenphysiol. 100: 147-160.
hy Mutants normal phy?
hy1 yes
hy2 yes
hy3 no
hy4 yes
hy5 yes
additional hy’s isolated later
hy8 no
Mutation in heme oxygenase (Davis et al., 2001)
mutation in heme oxygenase
mutation of phytochrome B
mutation of cryptochrome1, a photolyase-like protein
mutation in a B-zip transcription factor
mutation of phytochrome A (Dehesh et al, 1993)
Would turn out to encode:
7. Has topology similar to
histidine kinases, yet
exhibits ser-thr kinase
activity.
phytochrome binds ATP
diverged from prokaryotic
orthologs after duplication
of kinase transmitter domain
(Yeh and Lagarius, 1998).
BUT IS IT A KINASE?
8. Type I phytochrome :
photo-labile (phyA)
Type II phytochrome :
photo-stable (phyB,
phyC, phyD, phyE)
Two Major Types of Phytochrome:
B, R, FR
PrA PfrA
Dark reversion
Destruction
PrB PfrB
Dark reversion
R
FR
FR
9. The phy chromophore is a tetrapyrrole
that is reliant on heme oxygenase for its
synthesis; mutants in heme oxygenase
1 exhibit phy-like phenotypes.
The phenotypes can be rescued by
exogenous application of biliverdin
(Parks and Quail, 1991).
increasing BV
Brian Parks, circa 2000
and Brian’s homespun
Dobsonian mount telescope,
circa 2003.
10. Parks and Quail, 1993
Peter Quail
hy8 and hy1 mutants exhibit
specific inability to sense
far-red;
hy8 mutants sense red light
just fine…
11. Parks and Quail, 1993
The phenotypes of the phytochrome mutants
defined as hy loci.
Growth is unaffected in darkness.
hy3 and hy1 are impaired in red light
sensing
hy8 and hy1 are impaired in far-red light
sensing
12. Red – Import within minutes
Import of phyA to nucleus by red light also occurs within minutes; followed
by disappearance of the protein.
A phyB::GFP fusion protein is strongly
detectable in the nucleus of transgenic
tobacco cells beginning after three
hours (Gil et al., 2000).
13. Far-red– import after 1.5 h; retained
throughout time course (Hisada et
al, 2000).
phyA effects FR physiology with a
similar time course; protein constantly
detectable in WT background.
parks and spalding, 1999
14. Null phyB mutants are tested for complementation using the GR fusions.
The results indicate that both light and nuclear import are required for full
restoration of phyB activity.
15. Interaction between PIF3 and PHYB is dependent upon state of PHYB
PIF3 and PHYB
only interact if
phyB is in Pfr
state.
Ni et al., 1999
16. PIF3 Interaction with Promoter is Sequence Specific, and is Mediated
Through the DNA Binding Domains
Martinez-Garcia et al., 2000
17. Martinez-Garcia et al., 2000
Binding of phyB to the Promoter Requires PIF3, the phyB Chromoprotein
in the Pfr Form, and PIF3 Domains Outside of the DNA-Binding Domains
24. Cryptochromes
The sequence of Hy4 was reported in 1993, and it was similar to DNA
photolyase (Ahmad and Cashmore 1993, Sancar 1994) yet has no
photolyase activity.
Re-designated “Cryptochrome 1” (Lin et al., 1995)
Involvement in circadian rhythms; led to discovery of animal crys
(Cashmore, 2003)
Many plant responses were not R-FR
reversible and had action spectra with
peaks in the blue and near-UV. There
must be a BL receptor(s).
Due to their elusive nature, Gressel
(1977) described BL receptors as
“cryptochromes”.
hy4 mutants showed a lack of
hypocotyl growth inhibition under blue
light,but were normal under red and
far-red.
Dark BL
Wild-type
BL
cry1
adapted from Neff and Chory, 1998
25. Photoactivation of cryptochromes is (likely) similar to what
is observed in photolyases. Both a folate (MTHF) and a
flavin serve as chromophores in photolyase, and these
two compounds purify
with cry when it is expressed
in E.coli (Malorta et al.,
1995).
model of activity in photolyase
Cashmore, 2003.
26. cry2 mutants
The Cry2 gene was identified in the genome by homology to Cry1 (Jarillo et
al., 1996).
The cry2 mutants were isolated as seedlings not
expressing CRY2 protein. The mutants flowered late and
were shown to be allelic to other flowering-time genes
(below; Guo et al., 1998).
cry2 mutants lack a
conspicuous end-point
phenotype under high-
fluence blue light (right;
Mockler et al., 1998)
50 µmol m-2 s-1
5.5 µmol m-2 s-1
0.6 µmol m-2 s-1
27. CRY2 is phosphorylated in a light-dependent
manner (Shalitin et al., 2002)
1. Test for light-dependent phosphorylation
-- incubate with γ32P-ATP
2. Test to verify presence of CRY2 in same
conditions
3. Specific gel buffer/ionic
conditions allow resolution of the
phosphorylated form of the protein.
Again, the phosphorylated form (P)
is detectable only after BL
exposure.
P
28. The timing of CRY1 and CRY2
phosphorylation generally
coincides with CRY2’s influence
on early growth suppression.
Shalitin et al., 2002, 2003
Folta and Spalding, 2001
CRY2 phosphorylation
CRY1 phosphorylation
29. CRY2 accumulates in cop mutants
The phosphorylated
form accumulates in
cop1– and both the
phosphorylated and
unphosphorylated forms
disappear in WT. Propose
a mechanism.
30. Xing Wang Deng, Yale
COP Mutants
First isolated by Deng et al., 1991
--Constitutive Photomorphogenic
phenotype:
expanded cotyledons, short hypo-
cotyls, light-regulated gene
expression patterns in darkness
1996 Mayer et al show that COP1
mutation affects expression of
many genes
OLD SLIDE REVISITED!
31. The hy5 mutant – long hypocotyl under light conditions, particularly blue
(Koorrneef et al., 1980). Encodes a B-zip transcription factor that is
presumably a positive regulator of photomorphogenesis.
HY5 accumulates rapidly in light and is not
as detectable upon transfer of plants to
darkness
fluence rate
Plants grown for days in light show different
levels of HY5– HY5 level correlates with advanced
photomorphogenic development.
Osterlund et al., 2000
How does HY5 regulate photomorphogenesis?
Is it simply present only in light and acting as
a positive regulator? Is it more complex? It
seems to be acting in a manner that is
antagonistic to COP1….
32. HY5 mRNA accumulates in both
dark and light conditions, but the
protein is detected only in light.
Osterlund et al., 2000
Is this another example of ubiquitin-mediated proteolysis?
How can this be tested?
33. 1. Test using genetic tools – cop1 mutants
2. Test using
pharmacological tools
– proteosome inhibitors
Proteosome inhibitors allow
increased accumulation of
HY5.
mRNA levels are the same in cop and WT
plants in response to light treatment, yet
protein levels are very different.
This strongly suggests that COP1 has a role in
regulating the accumulation of HY5, a positive
regulator of photomorphogenesis.
Osterlund et al,. 2000
34. These results support a model where HY5, a positive regulator of
photomorphogenesis is degraded in darkness via COP1 and the
proteosome.
Osterlund et al., 2000
35. The C-Terminus of cry1 Regulates Photomorphogenesis
Ectopic overexpression of the CRY1
C-terminal extension results in a
constitutive-photomorphogenic
phenotype.
Cashmore, 2003
Is cry functioning through
a mechanism involving
COP1?
36. Does COP1 Interact with CRY?
Yeast 2-hybrid assay between COP1
truncations and CCT2 (CRY2 c-terminus)
Conclusions: The WD repeat domain is
necessary and sufficient for interaction,
yet binding is strongly enhanced by the
coiled-coil and Zn binding domains.
CCT2-GUS interacts similarly.
Coimmunoprecipitation –
Using anti-CRY2, COP1 can be
coimmunoprecipitated, demonstrating
likely interaction in vivo.
Wang et al., 2001
37. Hellmann and Estelle, 2002
Proposed Model for Cryptochrome Function – COP1, CRY, HY5
Interaction to Regulate Degradation of HY5
38. Phototropism
Noted by Darwin in observations of dark-grown canary grass
Colodny-Went model proposed that auxin would be secreted from the
tip and light would guide a lateral gradient of auxin to accumulate,
leading to differential growth.
A split in the plant biology community– phototropism just a phy response
vs a specialized BL receptor
39. Propose a Forward Genetic Screen - what might you find?
photoreceptor(s)– the actual molecule that receives the photon
signaling mutants– non-redundant genes required for transduction of the
signal from activated photoreceptor to target genes.
response mutants – those that have impaired cell wall expansion, auxin
transporters required for differential auxin redistribution… etc. ?
upstream elements – possibly molecules required for photoreceptor
synthesis and/or function.
40. phot1- BL-activated, membrane-associated, autophosphorylating ser/thr
kinase with two LOV domains (Christie and Briggs, 1998).
NPH3 - novel protein with possible BTB-POZ transcription activation
domains. NPH3 functions downstream of phot1, possibly participating as
a scaffold protein in the phosphorelay.
RPT2 – deficient in root phototropism, very similar to NPH3 .
NPH4- the same gene as ARF7 (Harper et al., 1999).
The phototropic mutant loci would be shown to encode:
41. Phototropins Regulate:
Phototropism (Huala et al., 1997; Christie et al., 1998)
Chloroplast accumulation and avoidance (Kagawa, 2001; Jarillo, 2001)
Primary hypocotyl growth inhibition (0-30 min; Folta and Spalding, 2001,
Folta et al., 2003)
Stomatal opening (Kinoshita et al., 2001)
Leaf expansion (Sakamoto and Briggs, 2002)
Blue-light destabilization of specific mRNA’s (Folta and Kaufman, 2003)
Solar tracking?
42. Phototropin – The Phototropism Photoreceptor
Mediates responses to blue and
near UV light.
Binds two flavin mononucleotide
molecules as chromophore
(Christie et al., 1998).
phosphorylation.. signaling or
desensitization?
Palmer et al., 1993
43. Phot Topology
Contains two specialized PAS domains called LOV domains (light, oxygen,
voltage) and a kinase domain (Christie and Briggs, 2001).
The protein can autophosphorylate on many sites (Salomon et al., 2003).
PHOT1 and PHOT2 share 60% sequence homology.
,
44. A Summary of Phot-Mediated Responses
phototropism chloroplast relocation stomatal aperture
low fluence-rate blue
high fluence-rate blue
Christie and Briggs, 2001
Sakai et al., 2001
accum avoid
45. Chloroplast accumulation and avoidance responses
LFR BL HFR BL
Treatment of leaf surfaces with low-fluence-rate blue light results in migration
of chloroplasts to the anticlinal surfaces of the leaf, perpendicular to incident
light. Irradiation with high-fluence-rate blue light results in migration of
chloroplasts to the periclinal cell surfaces, parallel to incident light.
This is important because it balances photosynthetic potential against
photoinhibition (Kasahara et al., 2002).
46. phot2 (aka. cav1 and npl1)
cav mutants obtained from forward
genetic screen for plants that failed
to show chloroplast avoidance.
Gene mapped to a region containing
NPL1, an NPH1 (phot1)- like gene.
A reverse genetic screen for NPL1
resulted in plants that failed to
relocate chloroplasts.
47. Photoreceptors of Adiantium
Adiantium contains 2 phototropins, and
shows normal phototropism and chloroplast
accumulation responses to BLUE LIGHT…
But the rap2 mutant does not exhibit
chloroplast accumulation or curvature in
response to red light.
-- Red light mediates phototropism and
chloroplast accumulation in Adiantium via a
hybrid phy-phot photoreceptor.
48. Cryptochrome mysteries to be resolved:
What is the mechanism of cry1 activation? How do redox changes affect
signaling?
cry1 and cry2 function within seconds of irradiation at the membrane, a
process that is required for inhibition of stem growth (Folta and Spalding,
2001). Do cry’s have a signaling mechanism independent of COP1?
cry interaction with phy, complete with cross phosphorylation has been
reported (Ahmad et al., 1997; Mas et al., 2000; Jarillo et al., 2000).
49. Integrated Scheme
of cry and phy function
Quail, April 2002
What has changed
already?
Don’t memorize this for
the exam! It may be very
different in a year!