Oocyte pick up and Embryo transfer
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This document discusses oocyte pick up and embryo transfer procedures. It describes the equipment, techniques, tips, and potential complications for oocyte pick up, which involves using ultrasound-guided needles to aspirate follicles and retrieve oocytes. It also outlines the timing, catheters, techniques, ultrasound guidance, and factors considered for embryo transfer, which involves placing embryos into the uterine cavity. Mock embryo transfers are recommended to practice catheter placement before the real procedure.
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This document discusses the technique of oocyte pickup (OPU). It begins by outlining the basic principles of the OPU technique, including proper patient and operator positioning, transducer handling techniques, and needle guidance. It then provides checklists for ensuring equipment is properly prepared and functioning before and after the procedure. Troubleshooting tips are provided for issues that may arise during OPU like failure of suction or no oocytes retrieved. Assessing competency and areas for future research in OPU techniques are also discussed.
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Improving embryo transfer technique is crucial in the field of assisted reproductive technology (ART) as it significantly influences the success of in vitro fertilization (IVF) procedures. A well-executed embryo transfer is vital for the optimal implantation of embryos, leading to a higher likelihood of a successful pregnancy. Here are several key strategies to enhance the embryo transfer technique:
Firstly, proper training and skill development for the fertility specialist or clinician performing the procedure are essential. Training programs that emphasize hands-on experience and guidance from experienced practitioners contribute to proficiency in embryo transfer. Continuous professional development ensures that practitioners stay updated on the latest advancements in the field.
Secondly, utilizing ultrasound guidance during embryo transfer enhances precision. Real-time visualization enables the clinician to accurately navigate the catheter through the cervix and deposit the embryos in the ideal location within the uterine cavity. This minimizes the risk of trauma and increases the chances of successful implantation.
Maintaining a relaxed and comfortable environment during the procedure is equally important. Studies suggest that minimizing stress and anxiety in both the patient and the clinician can positively impact the success of embryo transfer. This involves effective communication with the patient, addressing any concerns, and ensuring a supportive atmosphere in the clinic.
Optimizing the timing of embryo transfer concerning the woman's menstrual cycle is another critical factor. Synchronization between the embryo's developmental stage and the endometrial receptivity is vital. Personalized protocols and careful monitoring of hormonal levels contribute to better timing, enhancing the chances of successful implantation.
Lastly, considering individualized patient factors, such as uterine anatomy and the woman's overall health, is essential. Tailoring the embryo transfer technique to the specific needs of each patient increases the likelihood of a positive outcome.
In conclusion, continuous education, technological advancements, personalized approaches, and a patient-centered focus are key elements in improving the embryo transfer technique. Implementing these strategies can contribute to higher success rates in IVF procedures, bringing hope to individuals and couples seeking to build their families through assisted reproductive technologies.
OBSTETRICAL OPERATIONS & PROCEDURES.pptx
This document discusses various obstetric operations and procedures including induction of labor, forceps delivery, ventouse delivery, and version. It provides information on:
- The different methods of inducing labor, including medical, surgical, and combined methods using prostaglandins, oxytocin, amniotomy, and more.
- The prerequisites, steps, complications, and nursing role in induction of labor, forceps delivery, and ventouse delivery.
- The types, indications, contraindications, and procedures for external and internal versions.
Whopartograph
The document provides an overview of the WHO partograph, which is a graphical record used to monitor the progress of labor and the condition of the mother and fetus. It describes the history and development of the partograph, including Friedman's original design from 1954 and later additions by Philpott and Castle in 1972, who introduced the "alert line" and "action line". The document then explains the components, objectives, and proper use and interpretation of the partograph to monitor labor progress and determine if intervention is needed.
Whopartograph
The document provides an overview of the partograph, which is a graphical record used to monitor the progress of labor and the condition of the mother and fetus. It describes the history and development of the partograph, including Friedman's original partogram from 1954 and later modifications by Philpott and Castle that introduced alert and action lines. The WHO partograph is explained in detail, outlining its components for monitoring fetal condition, labor progress, and maternal condition. Guidelines are provided for normal labor progression and management based on the partograph, as well as how to identify and respond to abnormal labor progress. Key considerations for using oxytocin augmentation are also reviewed.
複製 Embryo-090423111342-phpapp02 (1)
This document discusses the process of embryo transfer in beef cattle. It involves collecting embryos from a superovulated donor cow through artificial insemination and flushing, and then transferring the embryos to synchronized recipient cows to complete gestation. The key steps are superovulating the donor cow, artificially inseminating her, flushing her uterus 7 days later to collect embryos, processing and evaluating the embryos, and then transferring high quality embryos into synchronized recipient cows 16 days after their estrus cycles have been aligned through hormone treatments.
Embryo 090423111342-phpapp02 (1)
This document discusses the process of embryo transfer in beef cattle. It involves collecting embryos from a superovulated donor cow through artificial insemination and flushing, and then transferring the embryos to synchronized recipient cows to complete gestation. The key steps are superovulating the donor cow, artificially inseminating her, flushing her uterus 7 days later to collect embryos, processing and evaluating the embryos, and then transferring high quality embryos into synchronized recipient cows 16 days after their estrus cycles have been aligned through hormone treatments. While expensive, embryo transfer allows for increasing the number of offspring from genetically superior cows and marketing their embryos.
Embryo 090423111342-phpapp02
This document discusses the process of embryo transfer in beef cattle. It involves collecting embryos from a superovulated donor cow through artificial insemination and flushing, and then transferring the embryos to synchronized recipient cows to complete gestation. The key steps are superovulating the donor cow, artificially inseminating her, flushing her uterus 7 days later to collect embryos, processing and evaluating the embryos, and then transferring high quality embryos into synchronized recipient cows 16 days after their estrus cycles have been aligned through hormone treatments.
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Menopause for the mrcog and beyond, second edition
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Ctg
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Oocyte pick up and Embryo transfer
- 1. Oocyte pick up and Embryo transfer Emad Darwish MD Professor of Obstetrics and Gynecology Integrated Fertility Centre Alexandria
- 2. Oocyte Pick up • Equipments • Technique • Complications • Approaches
- 3. Equipments • Ultrasound Machine with trans-vaginal probe. • Needle guide to be attached to the trans-vaginal probe. • Aspiration needle: single lumen or double lumen. • Follicle aspiration set.( ready made needle with tubing and collection tube) ready to be attached to suction pump. • Suction pump. ( can be replaced by 10 ml syringes).
- 4. Technique • General anaesthesia. Most common. • Aspiration of the follicles: Push needle through needle guide on the guide line. Push needle tip inside follicle and aspirate. All follicles to be aspirated. • Flushing of follicles: When no. of follicles less than 6-8. Up to 8 times till oocyte is retrieved.
- 5. Important tips • Tip of the needle should be seen all the times • Do not move probe side to side while needle inside ovaray. • Try to aspirate more than one follicle with single puncture in the vagina or the ovary. • Do not aspirate chocolate cysts and avoid to puncture it. • If bleeding from vaginal punctures, usually controlled easily by pressure and rarely needs diathermy. • Rotate needle when blocked by follicle wall.
- 6. Complications • Bleeding : Vaginal Intr-Abdominal • Infection • Pain • No oocytes
- 7. Approaches • Laparoscopic. • U/S guided: Trans-vaginal or Trans-abdomonal
- 10. Embryo Transfer • Timing • Catheters • Technique • Ultrasound guided • Bed rest after ET • Number of replaced embryos • Mock ET
- 11. Timing • Day 2-3 • Day 5-6
- 12. Catheters • Soft • Hard • Two steps
- 13. Technique • No need of anaesthesia. Very rare. • Clean cervix with saline. • Remove mucus from cervical canal. Not important • Flush cervix and cervical canal with culture media • Gentle manipulation • Tip of outer catheter just above internal os • Advance inner catheter to about 1-2 cm below upper end of cavity • Push piston and keep pressure for about 5-10 seconds • Withdraw catheter while keeping pressure
- 14. If difficult to pass internal os, shift to 2 steps transfer. • Use outer sheath with stellate and pass it from internal os. • Remove stellate • Advance inner catheter with embryos till 1-2 cm below fundus and replace embryos
- 15. Ultrasound • ET under U/S guidance improves pregnancy rate. • Full bladder for TAS • Very important for begginers • Needs expert assistant
- 16. Bed Rest • No evidence to support bed rest after ET .
- 17. Numbers of replaced embryos • Depends on many factors Age, history, embryo quality • 2-3 usual number. • Problem of multiple pregnancy • Fetal reduction • Freezing program • Cumulative pregnancy rate
- 18. Mock embryo transfer • On day of retrieval • Just before real ET • Before starting stimulation • eg 4+3 at 3 oclock shoulder of outer catheter at 4 cm, inner catheter pushed for 3 cm and introduction of catheter direction is at 3 oclock.