This document discusses different scales of fermentation including small, large, and pilot scales. Small scale fermenters are bench or lab scale systems that are smaller but highly automated and used as precursors for large plants. Pilot scale fermenters are small industrial systems used to generate information to design larger facilities. Large scale fermenters can have variable capacities and commonly use continuous stirred tank reactor designs. The document also covers the components, objectives, materials, and designs of fermentation systems at different scales.
A bioreactor is an installation for the production of microorganisms outside their natural but inside an artificial environment. The prefix “photo” particularly describes the bio-reactor's property to cultivate phototrophic microorganisms, or organisms which grow on by utilizing light energy.
These organisms use the process of photosynthesis to build their own biomass from light and carbon dioxide. Members of this group are Plants, Mosses, Microalgae, Cyanobacteria and Purple Bacteria.
Photobioreactor or PBR, is the controlled supply of specific environmental conditions for respective species.
Photobioreactor allows much higher growth rates and purity levels than anywhere in natural or habitats similar to nature.
The function of the bioreactor is to provide a suitable environment in
which an organism can efficiently produce a target product—the target product might be.
Cell biomass
Metabolite
Bioconversion Product
The performance of any bioreactor depends on the following key factors:
Agitation rate
Oxygen transfer
pH
Temperature
There is no universal bioreactor.
The general requirements of the bioreactor are as follows:
The design and construction of bioreactors must keep sterility from the start point to end of the process.
Optimal mixing with low, uniform shear.
Adequate mass transfer, oxygen.
Clearly defined flow conditions.
Feeding substrate with prevention of under or overdosing.
Suspension of solids.
Gentle heat transfer.
Compliance with design requirements such as: ability to be sterilized; simple construction; simple measuring, control, regulating techniques; scale-up; flexibility; long term stability; compatibility with up- downstream processes; antifoaming measures.
Microbial Kinetics in Batch Culture
Culture system containing a limited amount of nutrient, which is inoculated with the microorganism. Cells grow until some component is exhausted or until the environment changes so as to inhibit growth. Biomass concentration defined in terms of cell dry weight measurements (g/l) or total cell number (cells/ml).
Lineweaver-Burke Equation.....We remember the Monod Equation
Invert…
The equation now has the form of a straight line with intercept.
Y = MX + C
By plotting as a function of
You get a straight line, where the slope is , and the y–axis intercept is .
Product Yield Coefficient
Maintenance:
Cells use energy and raw materials for two functions, production of new cells and the maintenance of existing cells. In general, consumption of materials for maintenance is small w.r.t. the amount of materials used in the synthesis of new biomass.
Generally it is assumed that the use of materials for maintenance is proportional to the amount of cells present.
A bioreactor is an installation for the production of microorganisms outside their natural but inside an artificial environment. The prefix “photo” particularly describes the bio-reactor's property to cultivate phototrophic microorganisms, or organisms which grow on by utilizing light energy.
These organisms use the process of photosynthesis to build their own biomass from light and carbon dioxide. Members of this group are Plants, Mosses, Microalgae, Cyanobacteria and Purple Bacteria.
Photobioreactor or PBR, is the controlled supply of specific environmental conditions for respective species.
Photobioreactor allows much higher growth rates and purity levels than anywhere in natural or habitats similar to nature.
The function of the bioreactor is to provide a suitable environment in
which an organism can efficiently produce a target product—the target product might be.
Cell biomass
Metabolite
Bioconversion Product
The performance of any bioreactor depends on the following key factors:
Agitation rate
Oxygen transfer
pH
Temperature
There is no universal bioreactor.
The general requirements of the bioreactor are as follows:
The design and construction of bioreactors must keep sterility from the start point to end of the process.
Optimal mixing with low, uniform shear.
Adequate mass transfer, oxygen.
Clearly defined flow conditions.
Feeding substrate with prevention of under or overdosing.
Suspension of solids.
Gentle heat transfer.
Compliance with design requirements such as: ability to be sterilized; simple construction; simple measuring, control, regulating techniques; scale-up; flexibility; long term stability; compatibility with up- downstream processes; antifoaming measures.
Microbial Kinetics in Batch Culture
Culture system containing a limited amount of nutrient, which is inoculated with the microorganism. Cells grow until some component is exhausted or until the environment changes so as to inhibit growth. Biomass concentration defined in terms of cell dry weight measurements (g/l) or total cell number (cells/ml).
Lineweaver-Burke Equation.....We remember the Monod Equation
Invert…
The equation now has the form of a straight line with intercept.
Y = MX + C
By plotting as a function of
You get a straight line, where the slope is , and the y–axis intercept is .
Product Yield Coefficient
Maintenance:
Cells use energy and raw materials for two functions, production of new cells and the maintenance of existing cells. In general, consumption of materials for maintenance is small w.r.t. the amount of materials used in the synthesis of new biomass.
Generally it is assumed that the use of materials for maintenance is proportional to the amount of cells present.
Basic Knowledge about industrial microorganism. why industry choose microorganism rather than chemical. isolation technique of microorganism. source of microorganisms. Process of using microorganism. Disadvantages of using microorganisms in industry. Process of genetic modification of microorganisms. Storage process of microorganism. preservation methods of microorganism. Reculture methods of microorganism.
Bioprocess development and technology-Introduction,History of bioprocess,Milestones of Bioprocess development,Bioprocess development,Impact on Biotechnology
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
Basic Knowledge about industrial microorganism. why industry choose microorganism rather than chemical. isolation technique of microorganism. source of microorganisms. Process of using microorganism. Disadvantages of using microorganisms in industry. Process of genetic modification of microorganisms. Storage process of microorganism. preservation methods of microorganism. Reculture methods of microorganism.
Bioprocess development and technology-Introduction,History of bioprocess,Milestones of Bioprocess development,Bioprocess development,Impact on Biotechnology
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
The heart of the fermentation or bioprocess technology is the Fermentor or Bioreactor. A bioreactor is basically a device in which the organisms are cultivated to form the desired products. it is a containment system designed to give right environment for optimal growth and metabolic activity of the organism.
A fermentor usually refers to the containment system for the cultivation of prokaryotic cells, while a bioreactor grows the eukaryotic cells (mammalian, insect cells, etc).
‘Cider wine production using different yeast strains & comparing their efficiency, testing their antimicrobial activity towards E.coli’ fortification with honey for increasing vit b content and helping initial clarification. project author :Ritwik Bhatatcharya, MSc Food technology, BSc (hons)Microbiology. Done as part of Final smseter research project from Punjabi university , Patiala.wokring in QC(wine).Prodcution(FMCG), R&D. check website www.indobrews.com for more information,
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This PPT dicusses about the Stirred Tank Bioreactor and its features mainly used in Fermentation process.
Useful for students doing their Bachelor's in Life Science
fermentation, chemical process by which molecules such as glucose are broken down anaerobically. More broadly, fermentation is the foaming that occurs during the manufacture of wine and beer, a process at least 10,000 years old.
bioreactors and fermentors are culture systems to produce cells or organisms. They are used in various applications, including basic research and development, and the manufacturing of biopharmaceuticals, food and food additives, chemicals, and other products. A broad range of cell types and organisms can be cultivated in bioreactors and fermentors, including cells (like mammalian cell lines, insect cells, and stem cells), microorganisms (like bacteria, yeasts, and fungi), as well as plant cells and algae.Bioreactor and fermentor are two words for basically the same thing. Scientists who cultivate bacteria, yeast, or fungi often use the term fermentor. The term bioreactor often relates to the cultivation of mammalian cells but is also generically used.
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Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
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2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
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2. CONTENT
• Introduction
• Component of fermenter
• Scale of Fermentation
–Small scale fermentation
• Objectives
• Material and method
• Fermentation design
–Large scale fermentation
–Pilot scale fermentation
• Advantages & disadvantages of fermenter
3.
4. To produce microbial cells or biomass
To produce microbial enzymes.
To produce microbial metabolites.
To produce recombinant products.
To modify a compound which is added to the fermentation
( transformation)
5. FERMENTER
• A fermenter is basically a device in which the organism
are cultivated to form the desired product.
• It is containment system design to give right environment
for optimal growth and metabolic activity of the organism
6.
7.
8.
9. SCALE UP OF FERMENTER
Small scale
fermentor
Large scale
fermentor
Pilot scale
fermentor
10. SMALL SCALE FERMENTER
• Small scale fermenter are same as large fermenter (bioreactor).
• They are two type : bench scale or lab scale system.
• They are small but highly automated and customized for the application.
• Precursor of large plant.
• They can lab as CSTR or autoclave fixed, fixed on fluidized bed reactor application.
11. OBJECTIVE
• Minimize manual handling.
• Releasing economic on scale.
• Production if biopharmaceutical.
• Large scale experimentation are time consuming and expensive.
• They are use to exphase key variable before a move to large scale bioreactor is
made.
12. MATERIAL AND METHOD
• Tank- bioreactor
• Media- media for animal (serum, serum free)
for plant (MS, B5)
for microbes (urea enrichment)
• Power requirement-
• Inoculums- Inoculation of small scale fermenter of 250-
1000 liter capacity.
Fermenter inoculating using submerged inoculums for seed
fermenters.
13. • Maintains and optimum condition-
pH
Temperature
Agitation
Aeration
oxygen demand
• Harvesting – Product are two type-
Intracellular (Biomass)
Extracellular (Remove cell)
14. • If the product are extracellular, product is removal
of large solid particle and microbes cell usually be
centrifugation.
• The broth is fractionated or extracted in to major
fraction using ultracentrifugation, reverse osmosis,
gel filtration/ ion exchange/ adsorption.
15. FERMENTER DESIGN
• This largely determines the ranges of temperature and
pH that can considered, the degree of aerobiocity
required and the probable effect of contamination.
• The selection of fermenter configuration, i.e. a form of
batch stirred, continuous stirred tank or tubular
fermenter.
16. • The determination of fermenter dimension, e.g. volume
and diameter, and the values of the operating variables,
temp., and pH .
• The extent of the heat transfer surface and the mixing
devise required.
• Power and aeration requirements.
• The mechanical design, including selection of materials of
construction and maintenance of aseptic conditions.
17. SMALL SCALE FERMENTATION
DESIGN
• Two type of fermenter-
Bench fermenter
Lab scale fermenter.
Capacity-(1 – 100 liter).
Height Ratio 2:1.
Portable and fixed.
18. LARGE SCALE FERMENTER
• They are large capacity is variable.
• Many size variable.
• 2:1 ratio is common.
• Common type CSTR.
• E.g.:- bioreactor.
19.
20. PILOT SCALE FERMENTER
A pilot scale fermenter is a small
industrial system, which is operated to
generate information about the
behavior of the system for use
in design of larger facilities.
Pilot plant is a relative term in the
sense that plants are typically smaller
than full-scale production plants, but
are built in a range of sizes.