The document discusses bioprocess development and technology, outlining key terms, historical milestones, objectives, and processes involved in biotechnology. It highlights the stages of bioprocess development, including upstream and downstream processes, as well as challenges faced in the field, such as cost and competition with chemical industries. Additionally, it addresses the technological tools utilized in industrial biotechnology and the importance of improving sustainability and efficiency in agricultural practices.

1. Bioprocess development and
technology
Dr. Varsha C Mohanan
Assistant Professor
Department of Biotechnology
St. Mary’s College , Thrissur

2. Bioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s CollegeBioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s College
Introduction
Terms related to Bioprocess development:
Bioprocess technology- A technique that produces a
biological material
Industrial microbiology/ biotechnology- deals with all
forms of microbiology and biotechnology which have an
economic aspect.
Fermentation technology- deals with process and
techniques involved in fermentation, in which a substance
breaks down to simpler ones.
Biochemical engineering- is a field of study which involves
both chemical engineering and biological engineering.

3. History of bioprocess
Bioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s CollegeBioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s College
•History accounts way back to the documentation of production of
beer and bread in around BC 2500
Funerary stele from Amarna, circa 1350 BC.Credit: Mikhail Kokhanchikov/123RF
https://www.realmofhistory.com/2018/11/23/chronological-history-of-beer-facts/

4. Milestones of Bioprocess development
Bioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s CollegeBioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s College
https://www.slideshare.net/yongkangbirdnest/lecture-2-introduction-to-bioprocess

5. Bioprocess development
• Objectives of bioprocess
• Biomass production
• Enzyme production
• Metabolite production
• Recombinant protein production
• Metabolic biotransformation
Bioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s CollegeBioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s College

6. Products of Industrial biotechnology
Bioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s CollegeBioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s College
Primary
metabolites
Secondary
metabolites
Organic acids and
Polymers
Alcohols
Vitamins and
Nutraceuticals
Antibiotics
Biopharmaceuticals
Enzymes
SCPs, Biofuels and
Bioenergy
Fine and specialty
chemicals
New materials
Amino acidsMicrobial biomass
Microbial Enzymes
Recombinant
proteins
Biotransformation

7. Stages in Bioprocess development
• Upstream process- refers to the first step in which biomolecules are
produced by biotic systems (micro-organism, cell-lines, or single cell
chlorophytes) in bioreactors
• Downstream process- refers to the recovery and purification of biosynthetic
products, recycling salvageable components and proper effluent treatment
and disposal.
Bioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s CollegeBioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s College

8. Scale-up of Bioprocess
 The main problem area
• Inoculum development
• Medium sterilization
• Aeration- Agitation
 Stages of scale up
• Shake flasks
• Laboratory stirred Fermenters (Small bioreactors)
• Pilot scale Fermenters (Industrial bioreactors)
 The Monitor Experiment
Bioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s CollegeBioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s College

9. Bioreactor Design
Bioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s CollegeBioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s College

10. Tools of Industrial biotechnology
 Genetic Engineering
 Protein Engineering
 Metabolic Engineering
 New developments in Synthetic biology
 System biology- Omics and inSilico Approaches
Bioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s CollegeBioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s College

11. Challenges in Industrial bioprocessing
Bioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s CollegeBioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s College
•Agricultural raw materials are increasingly costly
•Low cost materials like cellulose cannot be readily
used for microbial process
•Bioprocess is still not effective as chemical
processing
•Chemical industry is also emerging competitive
•Large amount funding is needed for Bioprocess

12. •Make Agriculture more competitive and sustainable
•Improve quality of life of people while reducing impact by
development of innovative and affordable products.
•Helps increase industrial economy and environmental
efficiency and sustainability
Impact on Biotechnology
Bioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s CollegeBioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s College

13. REFERENCES
Bioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s CollegeBioprocess development and technology, Dr. Varsha C Mohanan, St.Mary’s College
• Principles of fermentation technology, Stanbury,P.F and
Whitaker
•Advances in Industrial Biotechnology, Chapter: 1 Industrial
Biotechnology: An Overview, I. K. International Publishingh
House Pvt. Ltd., New Delhi, Banglore, India Eds: Ram Sarup
Singh, Ashok Pndey, Christian Larroche
•Bioprocess technology, fundamentals and applications, KTH,
Stockholm