This document provides an overview of Myxomycotina (slime molds). It discusses that they are fungus-like organisms characterized by an amoeboid vegetative phase without cell walls. The document outlines the key characteristics and life cycles of the four classes: Acrasiomycetes, Hydromyxomycetes, Myxomycetes, and Plasmodiophoromycetes. It also briefly discusses their economic importance in nutrient cycling and use in laboratory studies due to their protoplasm without cell walls.
Heterothallic species have sexes that reside in different individuals. . The term is applied particularly to distinguish heterothallic fungi, which require two compatible partners to produce sexual spores, from homothallic ones, which are capable of sexual reproduction from a single organism.
Heterothallic species have sexes that reside in different individuals. . The term is applied particularly to distinguish heterothallic fungi, which require two compatible partners to produce sexual spores, from homothallic ones, which are capable of sexual reproduction from a single organism.
Introduction,In some fungi ,true sexual cycle comprising of nuclear fusion and meiosis is absent.
These fungi derive the benefits of sexuality through a cycle know as parasexuaL cycle.
First Reported by- Gudio Pontecorvo and J.A.Roper(1952)
Parasexual cycle was reported in
Aspergillus nidulans,the imperfect stage of Emericella nidulans.
Since then parasexual cycle has been discovered not only in several members of Deutromycetes but also in fungi belonging to Ascomycetes and Basidiomycetes.
DEFINETION - Parasexuality is defined as a cycle in which Plasmogamy, Karyogamy and Meiosis [Haploidization] take place in sequence but not at a specified time or at specified points in the life cycle of an organism.
Generally parasexual cycle occurs in those fungi in which true sexual cycle does not take place.
Parasexualcycle also know as Somatic recombination. PASEXUALITY ALSO REPORTED IN SOME ORGANISMS- Aspergillus nigar, Penicillium crysogenum, STEPS OF PARASEXUAL CYCLE - 1) ESTABLISHMENT OF HETEROKARYOSIS, 2) Formation of Heterozygous DIPLOIDS, 3) occasional mitotic crossing-over during multiplication of diploid nuclei, 4)occasional haplodization through aneuploidy , COMPARISION BETWEEN SEXUAL AND PARASEXUAL CYCLE, IMPORTANCE OF PARASEXUALITY, C0NCLUSION
This is an illustrated account for Unit 1 of Coure Course III Mycology and Phytopathology of Bsc Hons Program - Introduction to True fungi including characters, affinities, thallus, cell wall, nutrition and classification
The "Telome theory" of Walter Zimmermann (1930, 1952) is the most accepted theory that is based on fossil record and synthesizes the major steps in the evolution of vascular plants.
It describes how the primitive type of vascular plants developed from Rhynia like plants.
• Gymnosperms (Gymnos = naked, Sperma = seed) include the small group of plants with naked seeds.
• The Gymnosperms originated in the Devonian period of the Paleozoic Era and formed the supreme vegetation in the Mesozoic Era.
Introduction,In some fungi ,true sexual cycle comprising of nuclear fusion and meiosis is absent.
These fungi derive the benefits of sexuality through a cycle know as parasexuaL cycle.
First Reported by- Gudio Pontecorvo and J.A.Roper(1952)
Parasexual cycle was reported in
Aspergillus nidulans,the imperfect stage of Emericella nidulans.
Since then parasexual cycle has been discovered not only in several members of Deutromycetes but also in fungi belonging to Ascomycetes and Basidiomycetes.
DEFINETION - Parasexuality is defined as a cycle in which Plasmogamy, Karyogamy and Meiosis [Haploidization] take place in sequence but not at a specified time or at specified points in the life cycle of an organism.
Generally parasexual cycle occurs in those fungi in which true sexual cycle does not take place.
Parasexualcycle also know as Somatic recombination. PASEXUALITY ALSO REPORTED IN SOME ORGANISMS- Aspergillus nigar, Penicillium crysogenum, STEPS OF PARASEXUAL CYCLE - 1) ESTABLISHMENT OF HETEROKARYOSIS, 2) Formation of Heterozygous DIPLOIDS, 3) occasional mitotic crossing-over during multiplication of diploid nuclei, 4)occasional haplodization through aneuploidy , COMPARISION BETWEEN SEXUAL AND PARASEXUAL CYCLE, IMPORTANCE OF PARASEXUALITY, C0NCLUSION
This is an illustrated account for Unit 1 of Coure Course III Mycology and Phytopathology of Bsc Hons Program - Introduction to True fungi including characters, affinities, thallus, cell wall, nutrition and classification
The "Telome theory" of Walter Zimmermann (1930, 1952) is the most accepted theory that is based on fossil record and synthesizes the major steps in the evolution of vascular plants.
It describes how the primitive type of vascular plants developed from Rhynia like plants.
• Gymnosperms (Gymnos = naked, Sperma = seed) include the small group of plants with naked seeds.
• The Gymnosperms originated in the Devonian period of the Paleozoic Era and formed the supreme vegetation in the Mesozoic Era.
Mycology is the branch of biology concerned with the study of fungi.
The word 'myco' is derived from the Greek word mýkēs meaning “mushroom, fungus”.
Heinrich Anton de Bary is the father of Mycology.
Fungi are eukaryotic organisms that include such as yeasts, moulds and mushrooms. These organisms are classified under kingdom fungi.
Fungi are diverse and widespread.
This maybe of help for UG+PG Botany students studying mycology. It's about the general account of class Chytridiomycetes. Good for quick revision and information.
*Critics are very welcomed*
1) Strategies and structuresIn Protozoans the method of movement .pdfaptelecom16999
1) Strategies and structures:
In Protozoans the method of movement is determined by the type of organism and the
surrounding environment. Protozoans mainly move by cell extension, flagella or pseudopodia
and cilia, the movement as per the presence of structure can be classified as ciliary, flagellar and
amoeboid movement.
Ciliates : Ciliates form the largest group of protozoa. These organisms vary in size and often live
in watery environments, including oceans, marshes, bays and streams. Ciliates move using tiny
cilia, which are hair-like strands that act as sensors and tiny limbs.
Flagella are longer and less numerous that cilia, they use their long tail like flagella to move.
Amoebas : In these two cytoskeleton get polymerized . This creates a vacancy and cytoplasmice
material flow to cover the vacancy created. When amoeba moves cytoplasm moves to the arm
like extension called pseudopodium. This pseudopodium extends and enlarge and hence this
push the animal body towards that respective direction.
2) A) Flagellates can live as single cells, in colonies, or as parasites.
Commonly live in niche\'s of water.
They conduct photosynthesis and have a cell wall.
They contain flagella for propulsion or to create a current to bring in food.
They can inhabit the reproductive tract, alimentary canal, tissue sites and also the blood stream,
lymph vessels and cerebrospinal canal.
B) Pseudopods : Also called as false feet , are projections that can appear and disappear from the
organism\'s body. These are used for movement and to engulf prey and digest them using
enzymes.
C) Apicomplexa : Unicellular and spore forming, most of them possess a unique form of
organelle that comprises a type of plastid called an apicoplast, and an apical complex structure.
They have apicoplast(non photosynthetic plastid) , mitochondria and nuclear genomes.
Lack of cilia, sexual reproduction, use micropores for feeding, and the production of oocysts
containing sporozoites as the infective form.
They have unique gliding capability which enables them to cross through tissues and enter and
leave their host cells. This gliding ability is made possible by the use of adhesions and small
static myosin motors.
3) Key characteristics of fungi :
Fungi are unicellular or multicellular.
Most of the fungi grow as tubular filaments called hyphae
They are haploid.
Fungus are heterotrophs (they can obtain nutrients by absorption) . They absorb food and secrete
enzymes to digest complex molecules
Propogate by spores
Asexual or sexual reproduction
They can be multinucleated
Fungi are achlorophyllous (lack of cholorphyll pigment)
Both Fungi and protists belong to same kingdom but fungi is different from protist, protists are
able to live in an anaerobic environment without oxygen but fungi need aerobic respiration to
survive.
Protists are unicellular but fungi are multicellular. Protists are autotrophic (make their own
energy) and heterotrophic (rely on outside source to get energy), but fungi a.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
2. Introduction-
1.The Myxomycota or slime molds, are fungus-like organisms.
2.They are characterised by the absence of cell wall from their
amoeboid,’ animal-like vegetative or assimilatory phase.
3.The amoeboid assimilatory phase may consist of a free-
living multinucleate mass of protoplasm called a plasmodium
or simple uninucleate amoeboid cells, the myxamoebae (sing,
myxamoeba) often aggregating into a pseudoplasmodium or
amoeboid cells interconnected by slime filaments giving rise
to a structure known as net plasmodium or filoplasmodium.
4.They exhibit characteristic of both animals and plants.
5.The true slime molds are mostly saprophytic(formation of
3. fructifications) with a few parasitic ones.(absence of development of
fructifications, the plasmodium is converted into masses of spores).
6. All the true slime molds produce flagellated cells, the swarm cells.
7. The formation swarm cells is absent in the cellular slime molds. In the
cellular slime molds fructifications are developed from the
pseudoplasmodia.
8. In the net slime molds each cell is converted into a mucilaginous
sporocyte in which spores are developed.
9.The spores escape as biflagellate zoospores which after swimming for
some time, lose their flagella become surrounded by delicate envelope
and give rise to new net plasmodium.
10. They are found on moist dead wood, rotting logs, damp soil, leaf
mold, moist sawdust, bark of trees, decaying fleshy fungi, or other
4. organic matter. They often spend most of their lives within the
substrate and emerge only when about to produce sporangia.
Following periods of rainy weather they may occur on leaves
of grasses or other plants on lawns.
moist dead wood rotting logs damp soil, leaf mold,
moist sawdust bark of trees decaying fleshy fungi
on leaves of grasses or
other plants on lawns.
Other organic matter
5. Phylogeny-
•Macbride (1899) believed that the slime molds are fungi.
• Karling (1944) suggested that the slime molds are closely related to
Protozoa than to fungi.
•Martin (1932, 1960-61) has pointed out that if the algal origin of fungi
is accepted, then the slime molds are not fungi; but if the fungi have been
descended from colourless flagellates, the slime molds can well be
regarded as fungi.
• Sparrow (1958) proposed that the parasitic slime molds be regarded as
a separate Class the Plasmodiophoromycetes.
•Talbot (1971), Webster (1983), and others grouped all the four classes of
slime molds under the Division Myxomycota close to fungi.
•Bonner considers the Acrasiomycetes to have had their origin from the
free-living amoebae of the soil.
6. Classification- Kingdom Fungi
Division Myxomycota
(Plasmodium or pseudoplasmodium present) Eumycota
Classes
Acrasiomycetes Hydromyxomycetes Myxomycetes Plasmodiophoromycetes
(Amoeboid
cells
aggregating
into a
pseudoplasmo
dium)
(Amoeboid cells
spindle-shaped to oval
interconnected by
slime filaments
forming a net
commonly known as
‘net plasmodium’ or
‘filo-plasmodium’)
(Free-having
plasmodium bearing
haploid or diploid
nuclei, Plasmodium
non-parasitic,
fructification present.)
Plasmodium
parasitic,
fructification
lacking
7. A. Class: Acrasiomycetes:
Acrasiomycetes are commonly known as cellular
or amoeboid slime molds, and are found
profusely in the upper layer of humus in
deciduousforests and also in cultivated lands.
The characteristic features are:
1. Somatic phase commonly consists of amoeboid
cells or myxamoebae.
2. Myxamoebae aggregate to form a pseudoplasmodium, which develops fruit body.
3. Lack of flagellated cells, except in Pocheina rosea.
4. Spore wall contains cellulose.
5. Fruit bodies may be sorocarps (in Dictyostelium) or sporocarp (in Protostelium).
Raper (1973) divided the class into 3 subclasses: Protostelidae, Acrasidae and
Dictyostelidae.
Later, Olive (1975) raised the rank subclasses into classes as Protosteliomycetes,
Acrasiomycetes and Dictyosteliomycetes.
Reproduction- When resources such as water or food become limiting, the amoeba
will release pheromones such as acrasin to aggregate amoebal cells in preparation for
movement as a large (thousands of cells) grex or pseudopod. When in the grex, the
amoeboids reproduce, resulting in fruit-like structures called spores, which develop
into unicellular molds of the same species.
8. B. Class: Hydromyxomycetes:
The thallus forms net plasmodium or filoplasmodium. They are commonly aquatic
and saprobic, but rarely parasitic.
The characteristic features are:
1.The thallus consists of uninucleate spindle shaped cells, forming extensive
filaments.
2. The filaments are tubular and form net-like structure, the net-plasmodium or
filoplas- modium.
3. Reproduction by cyst formation, zoospore formation or by congregation.
The class consists of two orders Hydro- myxales and Labyrinthulales. But
Hawksworth et al. (1983) excluded Hydromyxales from fungi and included it in
Protozoans. The members of Labyrinthulales are parasitic on higher plants and also
on marine algae. Labyrinthula macrocystis is a very common species, found as parasite
on Zostera marina, the eel-grass.
9. Class-Myxomycetes /true slime molds /slime molds/ slime fungi /Mycetozoa. /
plasmodial slime molds (Fig. 4.10).
They are commonly found in damp places, especially on old wood and other
decomposing plant parts. T. Panckow in 1654 was the first to discover the
Myxomycetes.
The characteristic features are:
1. The vegetative body is a free-living plasmodium.
2. They feed on yeast cells, protozoa, fungal spores and other substances.
3. Reproduction takes place by asexual and sexual means. The reproductive process
is plantlike producing spores with definite cell walls.
4. Asexual reproduction takes place by fragmentation of plasmodium or binary
fission in myxamoebae.
5.Sexual reproduction takes place by fusion between flagellated zoospores or
myxamoeba to form zygote, from which multinucleate plasmodium develops by
mitotic divisions. They develop different types of fructification. These are
sporangium, aethalium and plasmodiocarp (Fig. 4.11D-G). Meiosis takes place
during spore formation in the fructification.
Alexopoulos (1973) divided the class Myxomycetes into three subclasses: Ceratio-
myxomycetidae, Myxogastromycetidae and Stemonitomycetidae.
10.
11.
12.
13. D. Class: Plasmodiophoromycetes:
This group is commonly known as endo- parasitic slime molds. They are obligate
parasites, grow on algae, aquatic fungi and higher plants (commonly in the roots).
The characteristic features are:
1. Members of this class are obligate (i.e., biotrophic) parasites of fresh water algae,
aquatic fungi and higher plants (commonly in the roots).
2. Somatic body consists of a naked holocarpic plasmodium.
3. Plasmodia are of two types in their life cycle: sporangiogenous plasmodium
(form sporangia) and cytogenous plasmodium (gives rise to cysts i.e., resting
spores).
4. Zoospores biflagellate, having unequal flagella of whiplash type, situated in
opposite direction, the shorter one in anterior and longer one in posterior side.
The class consists of a single order Plasmodiophorales with a single family Plasmo-
diophoraceae. The class consists of 16 genera and 45 species. Some like
Plasmodiophora brassicae causes club-root of crucifer; Spongospora subterranea
causes powdery scab of potato.
14. Sexual reproduction-
•The myxamoebae or swarm cells , behave as gametes and as such take part in the
sexual reproduction.
•Fusion may take place between the two swarm cells or two myxamoebae or between a
swarm cell and a myxamoeba resulting in the formation of a zygote, when plasmogamy
is followed by karyogamy.
•Fusion between the two swarm cells or between a swarm cell and a myxamoeba leads
to the development of a flagellated zygote which sheds its flagella and becomes
amoeboid.
• The zygote formed in any one of the processes grows, along with which the diploid
nucleus also divides mitotically giving rise to a multinucleate amoeboid structure, the
plasmodium.
•According to some, karyogamy takes place during zygote formation. To them, the
plasmodium represents the diplophase in the life history of a myxomycete and meiosis
takes place during the formation of spores in the fructification (fruit body).Whereas,
others are of opinion that karyogamy takes place just before meiosis during the
development of spores in the fructification and as such the plasmodium is in the
dikaryophasic condition.
15. Life cycle-
•The spores on germination produce one to four swarm cells or
myxamoebae.
•The swarm cells or myxamoebae behave as gametes. They fuse in pairs,
plasmogamy is followed by karyogamy. The zygote so formed grows
accompanied with repeated mitotic division of the diploid zygotic
nucleus resulting in the development of a plasmodium.
•Many zygotes may also coalesce to produce a plasmodium.
• A mature plasmodium thickens and gives rise to the fructification of
varied nature.
• During the development of the fructification (fruiting body) and spore
formation the diploid nuclei divide reductionally. Each haploid nucleus
with a portion of cytoplasm enveloped by a thick wall develops into a
spore.
•The spores vary greatly in colour and characteristics of the wall which
are of taxonomic importance.(Figure 327).
16.
17. Economic Importance of Myxomycota:
1.They have been the subject of intensive laboratory studies.
2. They contribute to the carbon and nitrogen cycles by
using various organic matter including bacteria as food.
3. They provide a large amount of protoplasm free from
cell walls which has been used as an ideal medium to solve
variety of fundamental problems of biochemists,
biophysicists, mycologists and even the geneticists.