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Mouse Microsurgery and 
echocardiography 
workshop 
Vahagn Ohanyan, MD, PhD 
vohanyan@neomed.edu 
Northeast Ohio Medical University 
Rootstown, Ohio, 44272
 Echo guided intra-myocardial and intra-ventricular 
injection 
 Stress echocardiography: measurement 
of cardiac function and blood pressure 
changes during stress test
Echo guided intra-myocardial and intra-ventricular injection 
A B 
Surgical towel Ultrasound 
scanhead 
Preparation of mouse:
Selection of needle and syringes
Intra-ventricular injection 
Volume for bolus injection: 
Mice: < 50 ul 
Rats~300g: <150 ul 
For small volume injection use Hamilton needle(27-30G) and syringes (sterilize before using) 
Hamilton 10 μl syrung C
Echo guided insertion of 27G needle 
27 G Needle The tip of the needle
LV Ventricle injection 
Video 
Before Needle inside the LV Cavity
Injection of saline into LV Ventricle 
Video
Intra-myocardial Injection 
• Use very small amount of solution to inject in 
one site. Large amount of the drug (solution 
can damage or rupture the ventricular wall). 
• For mice 10-15 ul in one site 
• For rats 20-30 ul (~300 g rat) 
• Sterilize the needle before insertion. 
• Prepare the animal as described in intra-ventricular 
injection section
Positioning needle inside the LV wall 
The tip of the needle LV anterior wall 
The tip of the needle 
A 
Video
Intra-myocardial injection 
Vedeo 
Needle is inside the LV cavity Needle inside the LV Wall
PV Loop catheter insertion into LV 
ventricle trough carotid artery 
• For carotid artery isolation, catheterization see 
page 53. 
• Clean the mouse chest for echocardiography as 
described in transthoracic echocardiography 
section. 
• Find parasternal long axis view and clear aortic 
root.
Parasternal long axis view 
Video
PV catheter inside the LV cavity 
Video 
Pressure-volume catheter 
Left ventricle
Incorrect and correct position of the 
probe 
Incorrect position Correct position
Stress echocardiography 
• If you want to remove vagal reflex, treat the 
animals with ganglionic blocker 
Norepinephrine or Dobutaminecontinuous 
injection is commonly used for experimental 
protocols. 
• Get all your measurement at plateau, which 
usually reaches after 30 second injection
Position of echo scanhead 
• After finding the desirable position (M mode 
or Doppler) fix the probe and do not change 
any adjustments. All this must be constant. 
Baseline During NE injection
SEE ONE , 
DO ONE , 
THEACH ONE, 
BECOME A CONTRIBUTRE
• For more information visit our website: 
• www.neomed.edu/microecho 
• https://www.linkedin.com/groups/Microsurgery- 
Small-Animal-Echocardiography- 
5022068?home=&gid=5022068&trk=my_groups-tile- 
grp 
• https://www.facebook.com/pages/Mouse- 
Microsurgery-and-Echocardiography- 
Workshop/398774443577599

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Mouse echocardiography, echo guided intraventricular and intramyocardial injection

  • 1. Mouse Microsurgery and echocardiography workshop Vahagn Ohanyan, MD, PhD vohanyan@neomed.edu Northeast Ohio Medical University Rootstown, Ohio, 44272
  • 2.  Echo guided intra-myocardial and intra-ventricular injection  Stress echocardiography: measurement of cardiac function and blood pressure changes during stress test
  • 3. Echo guided intra-myocardial and intra-ventricular injection A B Surgical towel Ultrasound scanhead Preparation of mouse:
  • 4. Selection of needle and syringes
  • 5. Intra-ventricular injection Volume for bolus injection: Mice: < 50 ul Rats~300g: <150 ul For small volume injection use Hamilton needle(27-30G) and syringes (sterilize before using) Hamilton 10 μl syrung C
  • 6. Echo guided insertion of 27G needle 27 G Needle The tip of the needle
  • 7. LV Ventricle injection Video Before Needle inside the LV Cavity
  • 8. Injection of saline into LV Ventricle Video
  • 9. Intra-myocardial Injection • Use very small amount of solution to inject in one site. Large amount of the drug (solution can damage or rupture the ventricular wall). • For mice 10-15 ul in one site • For rats 20-30 ul (~300 g rat) • Sterilize the needle before insertion. • Prepare the animal as described in intra-ventricular injection section
  • 10. Positioning needle inside the LV wall The tip of the needle LV anterior wall The tip of the needle A Video
  • 11. Intra-myocardial injection Vedeo Needle is inside the LV cavity Needle inside the LV Wall
  • 12. PV Loop catheter insertion into LV ventricle trough carotid artery • For carotid artery isolation, catheterization see page 53. • Clean the mouse chest for echocardiography as described in transthoracic echocardiography section. • Find parasternal long axis view and clear aortic root.
  • 13. Parasternal long axis view Video
  • 14. PV catheter inside the LV cavity Video Pressure-volume catheter Left ventricle
  • 15. Incorrect and correct position of the probe Incorrect position Correct position
  • 16. Stress echocardiography • If you want to remove vagal reflex, treat the animals with ganglionic blocker Norepinephrine or Dobutaminecontinuous injection is commonly used for experimental protocols. • Get all your measurement at plateau, which usually reaches after 30 second injection
  • 17. Position of echo scanhead • After finding the desirable position (M mode or Doppler) fix the probe and do not change any adjustments. All this must be constant. Baseline During NE injection
  • 18. SEE ONE , DO ONE , THEACH ONE, BECOME A CONTRIBUTRE
  • 19.
  • 20.
  • 21. • For more information visit our website: • www.neomed.edu/microecho • https://www.linkedin.com/groups/Microsurgery- Small-Animal-Echocardiography- 5022068?home=&gid=5022068&trk=my_groups-tile- grp • https://www.facebook.com/pages/Mouse- Microsurgery-and-Echocardiography- Workshop/398774443577599