Morphology Of Viruses : Viruses, structure and characteristics
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Viruses can be extremely simple in design, consisting of nuclei acid surrounded by the protein coat as a capsid. The capsid is composed of smaller protein components referred to as capsomeres. The capsid along with genome combination is called a nucleocapsid. The viruses can also posses additional components, with most common being an additional membranous layer that surrounds nucleocapsid called an envelope. This envelope is actually acquired from the nuclear or plasma membrane of the infected host cell and then Modified with viral protein called peplomere. A complete virus, with all the components needed for host cell to cause infection is referred to as virions. Viruses come in many shapes and sizes, but these are consistent and distinct for each viral family. The morphology of virus include size, shapes, genetic constituents, and the nuclear envelope An infective agent that typically consist of a nuclei acid molecule In protein coat, it is too small can not visible through naked eyes and even by light microscopy, and is able to multiply only within the living cells of a host. These are about 100 times smaller than bacteria and can only be observed by electron microscope. These are small obligate intracellular parasites, which Contain either a RNA or DNA genome surrounded by a protective, virus protein coat. These are acellular, so they are neither prokaryotes nor eukaryotes because they lack the characters of living beings except the ability to replicate. And they infect all types of cells :- humans, animals, plants, bacteria, yeast, protozoa, etc. VIRAL SIZE:- These are much smaller than bacteria for a time they were know as’filterable agents’ as they can pass through filters that can hold back bacteria. They can not be seen under light microscope hence called as'ultramicroscopic‘. These viral particles seen in this manner are know as ‘elementary bodies’. The size ranges:- 5-300nanometer. In recent years a number of viruses including mimivirus length of virus up to 600nm,and Pandoravirus Ranges from 50-100nm In length have been identified. Most viruses vary in diameter from 250-400nm;the largest, however measure about 500nm in diameter and are about 700-1,000nm in length. Paramyxoviruses can be up to 14,000nm long, Rotavirus particles measures 76.5nm in diameter. Viruses, VIRAL STRUCTURE, MORPHOLOGY
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Viruses are obligate intracellular agents that contain nucleic acids and have a protein capsid. They lack cell structures like cell walls and ribosomes. Viruses vary greatly in size and shape. The virus structure consists of the genome, capsid, and some have an envelope. The genome can be DNA or RNA. Capsids are protein shells that protect the genome and come in different symmetries like icosahedral or helical. Some viruses have an outer envelope of lipids and glycoproteins. Modern microbiology applies microbes to fields like medicine, biotechnology, food production, and more.
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This document provides an introduction to microbiology and common microorganisms. It discusses that microbiology is the study of microorganisms like bacteria, viruses, protozoa, and fungi. Most microorganisms are too small to be seen without a microscope. The document classifies microorganisms and describes some of the most common types like bacteria, viruses, protozoa, and fungi. It provides details on the structure and characteristics of different microorganisms and how they are identified.
Virus-Morphology, General structures and classification
This Power point presentation intends to explore a general structure and classification of virus, a biologist puzzle.
1 lecture virology 2021
The lecture provided an overview of virology, focusing on the physical structure and chemical composition of viruses. It discussed that viruses are submicroscopic organisms containing genetic material within a protein coat. It described the main structures of viruses, including the capsid, envelope, and viral genomes which can be DNA or RNA. The lecture also explained that viruses are composed of mostly proteins, which can be structural or enzymatic, and that the viral proteins and nucleic acids determine how viruses infect and exploit host cells.
Viruses – Morphology & Classification.pptx
This presentation gives a detail overview on Viruses - Morphology and Classification. The presentation is helpful for students of B. Pharm Second Year and those who wants to gain basic knowledge about Viruses.
Subject - Microbiology
Virus
1. The document discusses the structure and replication cycle of viruses.
2. Viruses consist of genetic material (DNA or RNA) surrounded by a protein coat called a capsid, and some have an outer lipid envelope.
3. Viral replication involves the virus entering the host cell, expressing its genes to produce viral proteins and genetic material, assembling new virus particles, and exiting to infect new host cells.
Unit i discovery of viruses ppt
This document discusses the discovery and structure of viruses. It describes how Ivanovsky and Beijerinck discovered viruses through filtration experiments in the late 1800s. Viruses were found to be filterable, invisible agents that could not be grown in culture. The structure of viruses is then explained, noting they contain nucleic acids surrounded by a protein capsid, and some have an outer envelope. Viruses are much smaller than bacteria and lack cellular structures like organelles.
Virology
Virology is the study of viruses, which are small particles that can only replicate inside living cells. Viruses are non-cellular and contain either DNA or RNA as their genetic material. They have capsids made of protein that surround their genomes and allow them to attach to and enter host cells. Viruses come in different shapes determined by the symmetry of their capsids, and some have envelopes containing glycoproteins. They are transmitted through various routes like direct contact, ingestion, inhalation, or mother-to-child. Viruses can be inactivated by physical means like heat and chemical treatments, though some may be resistant to certain solvents and disinfectants depending on their structure.
virology-201119022601 (1).pdf
Viruses were first discovered in the late 1880s through experiments with tobacco mosaic virus. Since then, many other viruses have been discovered that infect plants, animals and bacteria. Viruses are generally too small to be seen with a light microscope and have a variety of structures depending on their nucleic acid content and presence of an envelope. They replicate by infiltrating a host cell and using the cell's machinery to produce more viral particles. There is ongoing debate about whether viruses are considered living organisms.
Virology
Viruses were first discovered in the late 1880s through experiments with tobacco mosaic virus. Since then, many other viruses have been discovered that infect plants, animals and bacteria. Viruses are generally too small to be seen with a light microscope and have a variety of structures depending on their nucleic acid content and presence of an envelope. They replicate by infiltrating a host cell and using the cell's machinery to produce more viral particles. There is ongoing debate about whether viruses are considered living organisms.
Similar to Morphology Of Viruses : Viruses, structure and characteristics (20)
viruses_tobacco mosaic virus First semester part_1
viruses_tobacco mosaic virus First semester part_1
Virus-Morphology, General structures and classification
Virus-Morphology, General structures and classification
More from Shylesh M
Topological manupilation of DNA.pptx
The topology of DNA is defined by how the two complementary single strand are intertwined
DNA in a relax state usually assumes the B-Conformation with10.6 bp per turn
DNA is subjected to bends or opening of DNA over winding or unwinding, its base pair turn changes and the DNA is subjected to stress and strain
DNA Topology also encompasses super coiling
RNA – i PATHWAY
RNA interference [ RNAi] is a sequence mechanism , triggered by the introduction of ds RNA leading to mRNA degradation
It results in switching the targeted gene on and off at transcriptional or post transcriptional level3
The long double stranded RNAs enter a cellular pathway that is known as RNA interference pathway .
First ds RNAs get processed into 20-25 nucleotides small interfering RNAs [siRNAs] by an enzyme Dicer .
Small interfering RNAs assemble into RNA induced silencing complexes [RISCs] ,unwinding in the process.
The siRNAs strands subsequently guide the RISCs complementary RNA molecules , where they cleave and destroy the RNA .
Cleavage of RNA takes place near the middle of the region bound by siRNA strand .
This results into mRNA Degradation.
Gene knockdown
Double stranded RNA is synthesized with a sequence complementary to a gene of interest and introduced into a cell organism ,where it is recognized as exogenous genetic material and activates the RNAi pathway .
Using this mechanism, researchers can cause drastic decrease in the expresssion of targeted gene .
Since RNAi may not totally abolish expression of the gene , this technique is referred to as knockdown.
Glyoxylate cycle PATHWAYS REACTION
A series of metabolic reactions by which many different organism utilise fats for the synthesis of carbohydrate
Another Process Involving Glycolytic Enzymes and Metabolites
Anabolic metabolic pathway occurring in plants, and several
microorganisms , fungi not animals.
Occurs in glyoxysome
The enzymes common to the TCA cycle and the glyoxysomes are isoenzymes, one specific to mitochondria and the other to glyoxysomes.
The glyoxylate cycle allows plants to use acetyl-CoA derived from β-oxidation of fatty acids for carbohydrate synthesis (use fats for the synthesis of carbohydrates).
The glyoxylate cycle is a cyclic pathway that result in conversion of 2 carbon fragment of Acetyl CoA TO 4 carbon compound succinate then succinate is covert to oxaloacetate and then glucose involving the reaction of gluconeogenesis
VIRUSES CLASSIFICATION , LIFE CYCLE OF VIRUSES. CHARACTERISTICS OF VIRUSES
VIRUSES
LIFE CYCLE OF BACTERIOPHAGES
The word virus is derived from Latin word venom which means poisonous fluid that causes infection.
The branch of science that deals with the study of viruses is called Virology. It is the branch of Microbiology.
They show living characters inside the host and non living characters outside the host.
They contain either DNA or RNA as genetic material.
They have different size and shape. They cause diseases in plants, animals and micro-organisms .
Not cellular
Cannot carry on metabolic activities independently.
Contain either DNA or RNA, not both ( true cells contain both ).
Lack ribosomes and enzymes necessary for protein synthesis.
Reproduce only within cells they infect.
CLASSIFICATION OF VIRUSES
Holmes, in 1948, proposed a simple system of classifying viruses based on the type of cell (host) they infect:
Phytophagineae: They infect plants and they RNA as their genetic material. Eg: TMV,CaMV.
Zoophagineae: They infect animals and they have mostly DNA as their genetic material. Eg: Polio virus.
Pagineae: They infect bacterial cells, called bacteriophages they usually have DNA as genetic material.
Based on the viral envelope
Named after David Baltimore, a noble prize winning biologist n 1971.
1. dsDNA viruses Eg: Adenoviruses, Herpiviruses.
2. ssDNA viruses Eg: Paravoviruses.
3. dsRNA viruses Eg: Reoviruses.
4. (+)ssRNA viruses Eg: Picornaviruses.
5. (-)ssRNA viruses Eg: Orthomyxoviruses.
6. ssRNA-RT viruses Eg: Retroviruses.
7. dsDNA-RT viruses Eg: Hepadnaviruses.
Tobacco mosaic:
Causative agent: Tobacco mosaic virus (TMV)
Symptoms: The leaves of infected plants develop mosaic patches ,it is due to destruction of chlorophyll or due to production of abnormal chlorophyll .blisters appear in the region of dark green spots these may be regular or irregular in advanced stages leaves curl and get distorted.
Adsorption of the virion to the bacterial cell.
Penetration and decoating of the nucleic acid .
Protein synthesis.
Breakdown of bacterial DNA.
Arrest of host cell development.
Replication of phage DNA.
Maturation of infective progeny.
Lysis and release of newly formed phages.
Holmes, in 1948, proposed a simple system of classifying viruses based on the type of cell (host) they infect:
Phytophagineae: They infect plants and they RNA as their genetic material. Eg: TMV,CaMV.
Zoophagineae: They infect animals and they have mostly DNA as their genetic material. Eg: Polio virus.
Pagineae: They infect bacterial cells, called bacteriophages they usually have DNA as genetic material.
SDS- Polyacrylamide Gel Electrophoresis
SDS-Polyacrylamide Gel Electrophoresis
What is SDS?
Preparation of Gel
Process of SDS-PAGE
Visualization of protein bands
SDS-PAGE is differentiated into two systems.
*continuous sds-page
*discontinuous sds-page.
Polyacrylamide is used to form a gel, a matrix of a pores which allow the molecules migrate at different rates.
Negatively charged detergent sodium dodecyl sulfate.
Used to denature and linearize the proteins
Coated the proteins with negatively charged.
SDS-page is a technique that used to separate proteins according to their molecular size through the gel.
Proteins are unfolded and migrate from cathode to anode terminal at different rates.
Molecular weight is determined by compare the result with a standard curve of relative motility of standard proteins.
Visualizes the band under UV light.
Types of stains;
Coomassie Blue;
* Coomassie Brilliant Blue staining The Coomassie dyes R-250 and G-250 bind to proteins stoichiometrically through their sulfonic acid groups.
* . The interactions between dye and protein are Van der Waals and ionic. The sulfonic acid groups interact with positive amine groups. Therefore coomassie dye binds to wide range of proteins.
* Limited to ~100ng of protein.
Silver stain;
*most sensitive test
*detection limit 0.1-1.0ng of protein
The size of pores is determined by the concentration of acrylamide.
The higher the concentration, the smaller the size of pores.
Discontinuos sds-page consist of two different gels.
*stacking gel -4%of acrylamide
*separating gel-range from 5-15% of acrylamide.
SOLID WASTE AND MANAGEMENT BIO GAS PRODUCTION
Source And Management
Agriculture
Fisheries
Household
Commercial and Industry
MANAGEMENT :-
Storage
Collection
Transport and Handling
Recyling
Biogas production
Biogas production from biomass is an anaerobic process.
The anaerobic digestion is usually carried out by using are referred to as anaerobic digesters.
A digester may be made up of concrete bricks and cement or steel, usually built underground.
The digester has an inlet attached to a mixing tank feeding cow dung.
The methanogenic bacteria from another digester are also added with cow dung.
The digester is attached to a movable gas holding or storage tank with a gas outlet.
The used slurry comes out from the digester through an outlet. This can be used as a manure.
Process of Biogas production
By products of Sugar Industries
Molasses
Molasses is a viscous by product of refining sugarcane or sugar beets into sugar.
It contain solids, sucrose and reducing sugars.
Total sugar content is 45-55%. Hence it is a valuable raw material for the producton of many value added products.
India has the largest chemical industry in the world using sugarcane molasses to produce acetaldehyde, acetic acid, polyvinyl chloride, synthetic rubber etc.
Citric acid is produced easily from molasses by submerged fermentation.
Bagasse is the fibrous matter that remains after sugarcane stalks are crushed to extract their juice.
It is a dry pulpy residue left after the extraction of juice from sugarcane.
Bagasse is used as a biofuel and in manufacture of pulp and building materials.
STRUCTURAL ORGANIZATION OF PROTEINS
Primary structure of protein
Secondary structure of protein
Tertiary structure of protein
Quaternary structure of protein
Methods to determine protein structure
Conclusion
References
METHODS TO DETERMINE PROTEIN STRUCTURE
Each protein has a unique sequence of amino acids.
The amino acids are held together in a protein by
covalent peptide bonds or linkages.
A peptide bond are formed when amino group of an
amino acid combines with the carboxyl group of another.
The conformation of polypeptide chain by twisting or folding is referred to as secondary structure.
Two types of secondary structures α-helix and β-sheet are mainly identified.
α-Helical structure was proposed by Pauling and Corey in 1951.
It occurs when the sequence of amino acids are linked by hydrogen bonds.
Each turn of α-helix contains 3.6 amino acids.
β-pleated sheets are composed of two or more segments of fully extended peptide chains.
β-Sheets may be arranged either in parallel or anti-parallel direction.
Many globular proteins contain combinations of α-helix and β-pleated sheet secondary structure, these patterns are called supersecondary structures also called motifs.
The three dimensional arrangement of protein structure is referred to as tertiary structure.
It is a compact structure with hydrophobic side chains held interior while the hydrophilic groups are on the surface.
This type of arrangement provide stability of the molecule.
Besides the H-bongs, disulfide bonds, ionic interactions, hydrophobic interactions also contribute to the tertiary structure.
Biochemical Evidence For dna As Genetic Material
Biochemical Evidence For dna As Genetic Material
What is genetic material The genetic information in all cell is stored in DNA ,Discovery of Transformation in Bacteria , The transforming principle is DNA , HERSHEY & CHASE (1952) EXPERIMENT WITH T2 BACTERIOPHAGE, Summary of Hershey & Chase (1952 ) experiment
PRIONS AND VIROIDS
PRIONS AND VIROIDS , Diseases caused by Prions General properties , REPLICATION OF VIROIDS , viorids is 2 types
Vitamine B1 Thaimine Pyrophosphate
This document summarizes information about vitamin B1 (thiamine) and its active coenzyme form, thiamine pyrophosphate (TPP). It discusses the history of thiamine discovery and its chemical structure. Thiamine is converted to TPP in the liver and intestinal mucosa by the enzyme thiamine pyrophosphokinase using ATP. TPP acts as a coenzyme, transferring aldehyde groups in metabolic reactions. Sources of thiamine are mentioned and a deficiency can cause beriberi. Functions include roles in growth, nervous system maintenance, and metabolism.
STAINING TECHNIQUES AND TYPES PROCEDURE.
This document discusses various staining techniques used in microscopy to visualize bacteria and other microscopic organisms. It describes different types of stains including simple stains that color all structures the same and differential stains that color different structures differently. Specific staining techniques are explained, including Gram staining to distinguish between Gram-positive and Gram-negative bacteria, acid-fast staining for mycobacteria, and endospore staining. The document provides details on procedures, requirements, and results for common staining methods.
Capture of carbondioxide , entrapement of Co2
This document summarizes a seminar presentation on carbon dioxide (CO2) capture. It discusses how CO2 emissions have rapidly increased due to population growth and energy consumption. There are three main options to reduce CO2 emissions: improving energy efficiency, switching to non-fossil fuel energy sources, and developing carbon capture and storage (CCS) technologies. The document then describes the three main methods of CCS - pre-combustion, post-combustion, and oxyfuel combustion. It also discusses transporting the captured CO2 via pipelines and storing it underground through geo-sequestration in depleted oil fields, aquifers, and other rock formations. The advantages and disadvantages of CCS technologies are
FERMENTATION TYPES .
This document summarizes two main types of fermentation processes: solid state fermentation and submerged fermentation. Solid state fermentation occurs without free water and uses natural raw materials like grains as the carbon source to cultivate microorganisms. Submerged fermentation uses a liquid substrate and is best for microbes that require high moisture. Both methods have various applications, with solid state fermentation used for producing enzymes, biopesticides, and in bioremediation, while submerged fermentation is common in industrial manufacturing.
HUMAN CHROMOSOMAL ABERRATIONS AND KARYOTYPE ANALYSIS.
Chromosomal aberrations refer to changes in chromosome structure or number. The document discusses various types of structural aberrations like deletions, duplications, inversions, and translocations, and numerical aberrations involving changes in ploidy. Karyotype analysis involves staining and arranging chromosomes to identify aberrations. Abnormal karyotypes can lead to diseases like Down syndrome, Patau syndrome, and Cri du chat syndrome. The normal human karyotype contains 22 pairs of autosomes and an XX or XY sex chromosome complement.
More from Shylesh M (14)
VIRUSES CLASSIFICATION , LIFE CYCLE OF VIRUSES. CHARACTERISTICS OF VIRUSES
VIRUSES CLASSIFICATION , LIFE CYCLE OF VIRUSES. CHARACTERISTICS OF VIRUSES
HUMAN CHROMOSOMAL ABERRATIONS AND KARYOTYPE ANALYSIS.
HUMAN CHROMOSOMAL ABERRATIONS AND KARYOTYPE ANALYSIS.
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Authoring a personal GPT for your research and practice: How we created the Q...
Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.
The debris of the ‘last major merger’ is dynamically young
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
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because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
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s
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Brine shrimp (Artemia spp.) are used in marine aquaculture worldwide. Annually, more than 2,000 metric tons of dry cysts are used for cultivation of fish, crustacean, and shellfish larva. Brine shrimp are important to aquaculture because newly hatched brine shrimp nauplii (larvae) provide a food source for many fish fry (Mozanzadeh et al., 2021). Culture and harvesting of brine shrimp eggs represents another aspect of the aquaculture industry. Nauplii and metanauplii of Artemia, commonly known as brine shrimp, play a crucial role in aquaculture due to their nutritional value and suitability as live feed for many aquatic species, particularly in larval stages (Sorgeloos & Roubach, 2021).
Equivariant neural networks and representation theory
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
Sharlene Leurig - Enabling Onsite Water Use with Net Zero Water
Sharlene Leurig - Enabling Onsite Water Use with Net Zero WaterTexas Alliance of Groundwater Districts
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Isolation of pure culture, its various method.
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学校原件一模一样【微信：741003700 】《(salfor毕业证书)索尔福德大学毕业证》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
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Applied Science: Thermodynamics, Laws & Methodology.pdf
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.
The binding of cosmological structures by massless topological defects
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
Deep Software Variability and Frictionless Reproducibility
Deep Software Variability and Frictionless ReproducibilityUniversity of Rennes, INSA Rennes, Inria/IRISA, CNRS
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
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In this presentation include the prototype drug SAR on thus or with their examples .
Syllabus of Second Year B. Pharmacy
2019 PATTERN.
Compexometric titration/Chelatorphy titration/chelating titration
Classification
Metal ion ion indicators
Masking and demasking reagents
Estimation of Magnisium sulphate
Calcium gluconate
Complexometric Titration/ chelatometry titration/chelating titration, introduction, Types-
1.Direct Titration
2.Back Titration
3.Replacement Titration
4.Indirect Titration
Masking agent, Demasking agents
formation of complex
comparition between masking and demasking agents,
Indicators/Metal ion indicators/ Metallochromic indicators/pM indicators,
Visual Technique,PM indicators (metallochromic), Indicators of pH, Redox Indicators
Instrumental Techniques-Photometry
Potentiometry
Miscellaneous methods.
Complex titration with EDTA.
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- 1. MORPHOLOGY OF VIRUS Follow for More Biological Slides (LIFE –SCIENCE) Shylesh Murthy I A ( RESEARCHER) Passion About Genomics
- 2. HISTORY:- In 1897 Matinus Beijerinck coined the Latin name “VIRUS” meaning poison So he is considered as one of the founders of Virology. He studied filtered plant juice and found they caused healthy plants to become sick. They couldn’t be seen untill the electron microscope was invented in the 20th century
- 3. Wendell Stanley in 1935 crystallized sap from sick tobacco plant. He discovered viruses were made up of nuclei acid and protein. He stated that viruses were demonstrated to be a needle Shape crystalline particles, rather than a fluid.
- 4. INTRODUCTION:- An infective agent that typically consist of a nuclei acid molecule In protein coat, it is too small can not visible through naked eyes and even by light microscopy, and is able to multiply only within the living cells of a host. These are about 100 times smaller than bacteria and can only be observed by electron microscope. These are small obligate intracellular parasites, which Contain either a RNA or DNA genome surrounded by a protective, virus protein coat. These are acellular, so they are neither prokaryotes nor eukaryotes because they lack the characters of living beings except the ability to replicate. And they infect all types of cells :- humans, animals, plants, bacteria, yeast, protozoa, etc.
- 6. PROPERTIES:- Viruses can be extremely simple in design, consisting of nuclei acid surrounded by the protein coat as a capsid. The capsid is composed of smaller protein components referred to as capsomeres. The capsid along with genome combination is called a nucleocapsid. The viruses can also posses additional components, with most common being an additional membranous layer that surrounds nucleocapsid called an envelope. This envelope is actually acquired from the nuclear or plasma membrane of the infected host cell and then Modified with viral protein called peplomere. A complete virus, with all the components needed for host cell to cause infection is referred to as virions. Viruses comes in many shapes and sizes, but these are consistent and distinct for each viral family. The morphology of virus include size, shapes, the genetic constituents, and the nuclear envelope.
- 7. * VIRAL SIZE:- These are much smaller than bacteria for a time they were know as’filterable agents’ as they can pass through filters that can hold back bacteria. They can not be seen under light microscope hence called as'ultramicroscopic‘. These viral particles seen in this manner are know as ‘elementary bodies’. The size ranges:- 5-300nanometer. In recent years a number of viruses including mimivirus length of virus up to 600nm,and Pandoravirus Ranges from 50-100nm In length have been identified. Most viruses vary in diameter from 250-400nm;the largest, however measure about 500nm in diameter and are about 700-1,000nm in length. Paramyxoviruses can be up to 14,000nm long, Rotavirus particles measures 76.5nm in diameter.
- 13. REFERENCE:- 1. Medical virology by mishra b 2. Websites:- biology libre textbook 3. A textbook of biology ,by m. Sudhakar rao, cell biology and molecular biology 4. By Dr. Arumugam, saras publication. Seventh edition. 5. Www.Quantamagazine.Org/broadband-networks-of-viruses
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