1. Long-term memory is a system for permanently storing and retrieving information for later use. It codes information according to meaning, patterns, and other characteristics.
2. Tulving proposed that long-term memory consists of episodic, semantic, and procedural memory. Episodic memory stores autobiographical events, semantic memory stores general world knowledge, and procedural memory stores skills and how to perform actions.
3. Long-term memory is responsible for bringing continuity and meaning to life by storing information relatively permanently based on meaning and importance.
the presentation is about the encoding, capacity, retention duration, forgetting and retrieval of information in long term memory. it also introduce several studies done
the presentation is about the encoding, capacity, retention duration, forgetting and retrieval of information in long term memory. it also introduce several studies done
This content mainly is useful for various groups of people such as teachers, parents and others in making people or children remember well what do they learn in daily activities.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
3. MEMORY
• 1. THE ABILITY TO RECOVER INFORMATION ABOUT PAST EVENTS OR KNOWLEDGE.
• 2. THE PROCESS OF RECOVERING INFORMATION ABOUT PAST EVENTS OR
KNOWLEDGE.
• “MEMORY IS THE PROCESS OF MAINTAINING INFORMATION OVER TIME.” (MATLIN,
2005)
• “MEMORY IS THE MEANS BY WHICH WE DRAW ON OUR PAST EXPERIENCES IN ORDER
TO USE THIS INFORMATION IN THE PRESENT’ (STERNBERG, 1999).
• MEMORY IS THE TERM GIVEN TO THE STRUCTURES AND PROCESSES INVOLVED IN
THE STORAGE AND SUBSEQUENT RETRIEVAL OF INFORMATION.MEMORY IS
ESSENTIAL TO ALL OUR LIVES.
• WITHOUT A MEMORY OF THE PAST, WE CANNOT OPERATE IN THE PRESENT OR THINK
ABOUT THE FUTURE. WE WOULD NOT BE ABLE TO REMEMBER WHAT WE DID
YESTERDAY, WHAT WE HAVE DONE TODAY OR WHAT WE PLAN TO DO TOMORROW.
WITHOUT MEMORY, WE COULD NOT LEARN ANYTHING.
• MEMORY IS INVOLVED IN PROCESSING VAST AMOUNTS OF INFORMATION. THIS
INFORMATION TAKES MANY DIFFERENT FORMS, E.G. IMAGES, SOUNDS OR MEANING.
4. HOW DOES MEMORY WORK ?
HERE IS A SIMPLIFIED DESCRIPTION OF HOW MEMORY WORKS :
• ENCODING : THE INFORMATION GETS INTO OUR BRAIN A WAY THAT ALLOWS IS
TO BE STORED.
• STORAGE : THE INFORMATION IS HELD IN A WAY THAT ALLOWS IT TO LATER BE
RETRIEVED.
• RETRIEVAL : REACTIVATING AND RECALLING THE INFORMATION, PRODUCING IT
IN A FORM SIMILAR TO WHAT WAS ENCODED
5.
6. • MEMORY STORAG IS MADE UP OF THREE MEMORY SYSTEMS :
1. SENSORY MEMORY
2. SHORT TERM MEMORY
3. LONG TERM MEMORY
• SENSORY MEMORY - DURATION: ¼ TO ½ SECOND,
CAPACITY: ALL SENSORY EXPERIENCE (V. LARGER CAPACITY)
ENCODING: SENSE SPECIFIC (E.G. DIFFERENT STORES FOR EACH SENSE)
• SHORT TERM MEMORY - DURATION: 0-18 SECONDS
CAPACITY: 7 +/- 2 ITEMS
ENCODING: MAINLY AUDITORY
• LONG TERM MEMORY - DURATION: UNLIMITED
CAPACITY: UNLIMITED
ENCODING: MAINLY SEMANTIC (BUT CAN BE VISUAL AND AUDITORY)
7.
8. CHARACTERISTICS OF GOOD MEMORY
• IMMEDIATE UNDERSTANDING
• IMMEDIATE RECALL
• IMMEDIATE RECOGNITION
• STABILITY OF RECOGNITION
• FORGETTING USELESS THINGS
9. LONG TERM MEMORY
• LONG-TERM MEMORY: A SYSTEM FOR PERMANENTLY STORING, MANAGING,
AND RETRIEVING INFORMATION FOR LATER USE. ITEMS OF INFORMATION
STORED AS LONG-TERM MEMORY MAY BE AVAILABLE FOR A LIFETIME.
• LTM CODES INFORMATION ACCORDING TO MEANING,PATTERN AND OTHER
CHARACTERISTICS
• IT BRING CONTINUITY AND MEANING IN OUR LIFE.
• STORING INFORMATION RELATIVELY PERMANENTLY .
• STORING ON BASES OF MEANING AND IMPORTANT.
• FUNCTION : ORGANIZES AND STORES INFORMATION MORE PASSIVE FROM
STORAGE THAN WORKING MEMORY
10. • ENCODING : PROCESS THAT CONTROLS MOVEMENT FROM WORKING TO LONG
TERM STORE.
• RETRIEVAL : PROCESS THAT CONTROLS FLOW OF INFORMATION FROM LONG
TERM TO WORKING MEMORY STORE
11. • LONG-TERM MEMORY WAS PROPOSED BY TULVING (1972).
• HE PROPOSED A DISTINCTION BETWEEN EPISODIC, SEMANTIC AND PROCEDURAL
MEMORY.
• PROCEDURAL MEMORY - PROCEDURAL MEMORY IS A PART OF THE LONG-TERM
MEMORY IS RESPONSIBLE FOR KNOWING HOW TO DO THINGS, I.E. MEMORY OF
MOTOR SKILLS . IT DOES NOT INVOLVE CONSCIOUS (I.E. IT’S UNCONSCIOUS -
AUTOMATIC) THOUGHT AND IS NOT DECLARATIVE. FOR EXAMPLE, PROCEDURAL
MEMORY WOULD INVOLVE KNOWLEDGE OF HOW TO RIDE A BICYCLE.
• SEMANTIC MEMORY - SEMANTIC MEMORY IS A PART OF THE LONG-TERM MEMORY
RESPONSIBLE FOR STORING INFORMATION ABOUT THE WORLD. THIS INCLUDES
KNOWLEDGE ABOUT THE MEANING OF WORDS, AS WELL AS GENERAL
KNOWLEDGE.FOR EXAMPLE, LONDON IS THE CAPITAL OF ENGLAND . IT INVOLVES
CONSCIOUS THOUGHT AND IS DECLARATIVE . THE KNOWLEDGE THAT WE HOLD IN
SEMANTIC MEMORY FOCUSES ON “KNOWING THAT” SOMETHING IS THE CASE (I.E.
DECLARATIVE). FOR EXAMPLE, WE MIGHT HAVE A SEMANTIC MEMORY FOR
KNOWING THAT PARIS IS THE CAPITAL OF FRANCE.
12. • EPISODIC MEMORY - EPISODIC MEMORY S A PART OF THE LONG-TERM MEMORY
RESPONSIBLE FOR STORING INFORMATION ABOUT EVENTS (I.E. EPISODES) THAT
WE HAVE EXPERIENCED IN OUR LIVES. IT INVOLVES CONSCIOUS THOUGHT AND
IS DECLARATIVE .
AN EXAMPLE WOULD BE A MEMORY OF OUR 1ST DAY AT SCHOOL . THE
KNOWLEDGE THAT WE HOLD IN EPISODIC MEMORY FOCUSES ON “KNOWING THAT”
SOMETHING IS THE CASE (I.E. DECLARATIVE). FOR EXAMPLE, WE MIGHT HAVE AN
EPISODIC MEMORY FOR KNOWING THAT WE CAUGHT THE BUS TO COLLEGE
TODAY.
13. • COHEN AND SQUIRE (1980) DREW A DISTINCTION BETWEEN DECLARATIVE
KNOWLEDGE AND PROCEDURAL KNOWLEDGE.
• PROCEDURAL KNOWLEDGE INVOLVES “KNOWING HOW” TO DO THINGS. IT
INCLUDED SKILLS, SUCH AS “KNOWING HOW” TO PLAYING THE PIANO, RIDE A
BIKE; TIE YOUR SHOES AND OTHER MOTOR SKILLS.IT DOES NOT INVOLVE
CONSCIOUS THOUGHT (I.E. ITS UNCONSCIOUS - AUTOMATIC). FOR EXAMPLE,
WE BRUSH OUR TEETH WITH LITTLE OR NO AWARENESS OF THE SKILLS
INVOLVED.
• DECLARATIVE KNOWLEDGE INVOLVES “KNOWING THAT”, FOR EXAMPLE LONDON
IS THE CAPITAL OF ENGLAND, ZEBRAS ARE ANIMALS, YOUR MUMS BIRTHDAY
ETC. RECALLING INFORMATION FROM DECLARATIVE MEMORY INVOLVES SOME
DEGREE OF CONSCIOUS EFFORT – INFORMATION IS CONSCIOUSLY BROUGHT TO
MIND AND “DECLARED”.