This document summarizes the education and research experience of Jaeho Lee. He earned a B.S. in Environmental Horticulture from the University of Seoul and an M.S. in Entomology from Seoul National University. For his graduate thesis, Lee developed a film-assisted honeybee egg collection system to improve honeybee genome editing techniques. He then used these techniques to successfully knockout the nAChR alpha6 gene in honeybees, proving the concept of creating pesticide-resistant honeybees. Lee has also published research on head louse adhesion proteins and acetylcholine esterase paralogs in bedbugs and honeybees.

1. Introduction to
the Specialty of
Jaeho Lee

2. I. Higher Education
Classes Contents
Undergraduate
school
Plant genetics • Basic knowledge of genetics and molecular biology
Experiment of
Molecular Biology
and Microbiology
• Micro organism cultivation and aseptic techniques
• Optical microscopy, blood type detection using PCR.
Molecular
Biotechnology
• Explains concepts and principles of various
experimental techniques
• qPCR, sequencing, phage display, protein synthesis
• aptamer screening, MS, MAB, etc.
(EX) BIO-410:
Manipulation of
Recombinant DNA
• Practice techniques required for basic molecular
biology research.
• PCR, cloning, protein expression, western blot, etc
• Practicing in group of two person. Lab note writing
and review.
⁞ ⁞
Graduate
school
Genetic
Engineering
• Explain genetic engineering techniques in business-
oriented perspective. Development history of
techniques, patent strategy etc.
Animal Cell
Culture
• Cell Biology (adhesion, proliferation, cycle, signal
transfer, metabolism etc.)
• Cell Culture related techniques (gears, containers,
media, cell types, charactorization, selection,
separation etc)
Insect Pathology
• Explains entomopathogenic micro-organisms and
baculovirus expression system
⁞ ⁞
- B.S at University of Seoul (Environmental horticulture Major)
- Exchange Student at North Carolina State University (spring 2015)
- M.S at Seoul National University (Entomology Major in Department of
Agricultural Biotechnology)
- Attended multiple lectures held by department of life science
Table of important classes
2

3. II. Graduate School Experience & Thesis
- Affiliation: Lab of Insect Molecular Biology and Toxicology
- Thesis: Development of Film-assisted Honeybee Egg Collection System (FECS) and
Its Application to Honeybee Genome Editing.
- Thesis Summary: Improve honeybee genome editing protocol and prove
concept of pesticide resistant honeybee
PART1: Film-assisted Honeybee Egg Collection System (FECS)
Developed massive honeybee egg(embryo) collection system exploiting 3D
printing technique and silicone coating. Expected to be utilized in various
honeybee research such as toxicity evaluation and developmental biology
aside from genome editing
* Published in Apidologie (SCI journal) as the first author.
Silicone coated
film-attaching
surface
Queen excluder
Comb box
Transparent film
a
System and components design
Post-processing and
Operatoin
3D printing
Efficient research enabled
by massive collected
embryos 3

4. II. Graduate School Experience
PART II: Knock Out of Honeybee nAChR alpha6 gene
Edited honeybee genome to prove the concept of pesticide resistant honeybee
which can be free from the fear of residual pesticides (in agricultural
environment) and parasitic mites resistant to conventional miticides. nAChR
alpha 6 gene (which express the target protein of the pesticide Spinosad) was
successfully knocked-out (confirmed knock-out from F1)
* nAChR is Cation Channel acting on neurons and consist of five subunits. Knock-Out or G275E point mutation
on alpha6 subunit gene is a well-known inducer of Spinosad resistance in Insects.
E
A v e ra g e A g e o f E m b ry o a t in je c tio n (h )
H
a
t
c
h
in
g
R
a
t
e
(
%
)
0 3 6 9 1 2 1 5
0
2 0
4 0
6 0
8 0 P o s te rio r In je c tio n
M e d ia l in je ctio n
Analyze existing method
Theoretic optimization of
microinjection through
fluorescent microscopic
observation of early embryos
Dupuis et al, 2012
Compare survival rate depending on
injection site and time
Repeat microinjection
4
II. Graduate School Experience & Thesis

5. G S Y F N C I M F M V A S S L T V L V
WT
12-20
18-1
18-2
18-23
Transmembrane Domain 3
G275
18-23
Number of
Injected
Embryos
Hatched
Larvae
Larvae introduced
into colony
Emerged
Queens
Queens with
offspring
Queens with genome edited of
fspring
1909 272 (14.2%) 91 15 (16.5%) 3 2 (66.7%)
Confirmation of F1 Knock-Out through DNA sequencing and the result
of experiment.
Follow-up studies
Reproducing one-step knock-out introduced in recent studies (Queen
Knock-Out of white gene)
5
II. Graduate School Experience
II. Graduate School Experience & Thesis

6. • Identification and Analysis of Headlouse Knit Adhesion Protein
-Scientific Reports volume 9, Article number: 48 (2019)
• Study of Acetylcholine Esterase Paralogs in Bedbug and Honeybee
-Scientific Reports volume 9, Article number: 10342 (2019)
• Study of pest management using RNAi (thrips, two-spotted spider mites)
• Research to discover unique antigen to detect pine wilt
III. Other Graduate School Studies
IV. External Training
2017. Jul 22-27
IGTRCN Technical workshop held at University of Maryland
- completed 50-hour course on Transgenesis and Genome editing
6