A recent version of my CV (no address)

1. Jason C. Poole, PH.D
Tel (949) 302-9980 jasoncpoole@gmail.com
Profile
I am a molecular biologist with 17 years experience developing novel molecular and genetic assays for
research and diagnostic use. My research background is diverse spanning aging, cancer, infectious
disease and mitochondrial genetics. My work in these fields has focused on signal transduction and gene
regulation. I have extensive technical skills and personnel management experience in industry and
academia. My technical expertise includes qPCR, Next Generation Sequencing, SNP analysis, Nucleic
acid prep and molecular cloning strategies (extraction/purification, expression constructs, transgene
expression systems). I’ve also performed high throughput screening in mammalian cells.
My most recent experience at EMD Millipore involves commercial product development for the qPCR and
NextGen Sequencing markets. My team successfully developed and released qPCR-based RNA
expression assays for stem cell and mitochondrial research. In the Next Generation Sequencing space
we recently completed the development of a DNA sample prep and validation solution for DNA
sequencing libraries. These kits are sold in the United States and international markets.
I’m currently seeking a project leader or senior scientist position in an intellectually stimulating
environment. My previous experience in developing molecular assays would be a strong asset in a
diagnostic development environment, particularly in the qPCR or Next Generation Sequencing fields. I
encourage you to contact me to discuss our common goals and interests and believe my skills,
knowledge, and enthusiasm for working in a collaborative environment fit the profile of someone who can
make a positive contribution to your company. I look forward to hearing from you.
Experience
July2009-Present EMD Millipore Inc. Senior Scientist
Lab supervisor for the molecular biology group with direct and indirect personnel management
responsibilities. I lead the development of qPCR, expression and next generation sequencing assays. I’m
responsible for many aspects of product development from concept and market assessment to R&D and
product launch activities. Other responsibilities include QC assay development and optimization,
troubleshooting current product manufacturing issues, and the technology evaluation for novel qPCR and
molecular genetic technologies.
Aug2008-July09 Nanogen Inc. Senior Development Scientist
Process optimization for a high-throughput clinical diagnostic assay. Development of an influenza pPCR
diagnostic test founded on proprietary minor groove binding probes. Development and troubleshooting
of a diagnostic testing platform using microfluidic reaction cartridges and a microthermal circuitboard.
Managing research associates in a BSL-2e facility and molecular testing environment working with live
Influenza virus. Excelling in a fast-paced environment and working under deadlines for a CDC contract.
At Nanogen I honed my experimental troubleshooting skills through the need for strict controls and
reporting requirements as we moved toward the approval of a molecular diagnostic device for clinical
diagnostic use (510k).
2004-2008 University of California, Irvine. Postdoctoral Researcher
Studies on the physiological and genetic changes of mitochondria as a result of adaptive selection and
human migration. I developed in vitro screening assays for investigating physiological facets of
mitochondrial function (membrane potential, free radicals, oxygen utilization) in human embryonic stem
cells and other human cell lines. I also development a clinical diagnostic assay for use in the genetic
diagnosis of mitochondrial disease. Directed the Center for Mitochondrial Medicine’s human cell culture
facility.

2. 2000-2001 Integrated Genomics Inc. Senior Research Associate
High-throughput sample handling for the sequencing and genomics group using a CRS robotics platform.
Operated high-throughput robot table containing orbital stackers, automated liquid handler, plate reader
and thermocycler and capable of a variety of genetic and molecular assays.
1998-2004 University of Illinois College of Medicine, Chicago. PhD Candidate
Biomarker discovery of cell cycle checkpoint genes through microarray screens of human tumor cells.
WAF1
Investigation of the effects of p21 mediated changes in senescence related gene expression.
Identified cis-acting promoter elements in novel genes regulated by p21 and identified transcription
factors important in their regulation. Confirmed the validity of these biomarkers with retroviral expression
vectors and development of an in vitro small molecule screening platform.
1996-1998 Northwestern University, Evanston, IL. Masters Student
Characterization, sequencing and cloning of mouse placental hormones identified through a
bioinformatics search for homologous proteins and cloned with the use of custom degenerate primers.
Education
2004 Doctorate of Philosophy Molecular Genetics, University of Illinois, College of Medicine, Chicago.
1998 Master of Science Molecular Biology, Northwestern University Evanston, IL.
1995 Bachelor of Arts Behavioral Neuroscience, University of Colorado, Boulder, CO.
1994 Study Abroad, German/Psychology, Universitat Regensburg, Germany.
Skill Sets
Biomarker Discovery
Next Generation Sequencing and analysis (MiSeq and Galaxy software)
Experience with compound screening in mammalian cells in 96-well formats
Identification of drug sensitive biomarkers from a microarray screen in human tumor cells.
Expert in the design and implementation of gene expression experiments.
Development of biochemical and kinetic in vitro cellular assays to confirm biomarker function.
Experience with inducible genetic systems using retroviral transduction, mammalian cell
transfection, electroporation, gene reporter assays and stable transgenic cell development.
Experienced with high throughput environments in an industrial setting. Developed high-
throughput assays on a robotics table for use in biological applications.
Molecular Diagnostics/Genotyping
SNP biomarker identification for use in human clinical diagnostics.
10 years experience in human cell culture and the development of cell based screening assays
including the development of novel flow cytometric assays.
Extensive experience in the SNP data analysis for clinical diagnostic use including the
development and design of multiplex primer extension assays.
Design and implementation of a clinical diagnostic assay for use in a high complexity CLIA
certified laboratory.
Co-author of one of the largest published trees of human mitochondrial polymorphisms
including clinically relevant SNPs.
Management Experience
Project team leader for Commercialized Genetic Assays
Planning and troubleshooting experiments and guidance of junior scientists in the development
of molecular diagnostic assays

3. Establishment and management of a BSL2+ and stem cell facility utilized by a dozen research
associates
Successful competition for government grants. Authoring progress reports for submission to a
granting institution. Complete analysis and synthesis of data from our science group each
month.
Grant Awards
2004-2007. NIH F32 NRSA Postdoctoral Training Award: National Institute on Aging. Project Title:
Mitochondrial Regulated Longevity Assurance Genes.
2005-2007 UC, Irvine Campus Representative for the California Council of Postdoctoral Scholars.
2002 Ellison Medical Foundation New Scholars Award: Molecular Biology of Aging.
2002 Scholar Travel Award: Buck Institute Symposium on Age Research.
2001 Molecular Biology of Aging Scholarship; Marine Biological Labs, Woods Hole, MA.
1999-2001 NIH T32 doctoral training grant: Signal Transduction and Cellular Endocrinology.
Patents
Igor Roninson, Jason C. Poole, and Bey-Dih Chang (2001). WO/2003/073062 Reagents and Methods for Identifying and Modulating
Expression of Genes Regulated by CDK Inhibitors.
Jason C. Poole and Adrian Vilalta (2012). WO/2012/097849 Mitochondrial Genome-Restricted DNA Sequences.
Publications
Jason C. Poole, Tony Gaige, Jackie Stupack, Nicole A. Leonetti, Adrian Vilalta (2011). Biomarker fur den Zustand einer Zelle (Biomarker
for the state of a cell).Nachrichten aus der Chemie 59(10). 983-984.
Jason C. Poole, Vincent Procaccio, Martin C. Brandon, Greg Merrick, Douglas C. Wallace (2010). Multiplex Analysis of
mitochondrial DNA pathogenic and polymorphic sequence variants. Biological Chemistry. (ePub October 2010).
Suissa S, Wang Z, Poole J, Wittkopp S, Feder J, et al. 2009 Ancient mtDNA Genetic Variants Modulate mtDNA Transcription and
Replication. PLoS Genet 2009 5(5): e1000474.
Sher L. Hendrickson, Lawrence Kingsley, Eduardo Ruiz-Pesini, Jason C. Poole, Douglas C. Wallace and Stephen J. O’Brien.
Mitochondrial DNA Haplogroups influence lipoatrophy after Highly Active Anti-retroviral Therapy. JAIDS. 2009 Jun 1;51(2):111-6.
Hendrickson SL, Hutcheson HB, Ruiz-Pesini E, Poole JC, Wallace DC, O'Brien SJ. Mitochondrial DNA haplogroups influence AIDS
progression. AIDS. 2008 Nov 30;22(18):2429-39.
S. Bannwarth, V. Procaccio, C. Rouzier, K. Fragaki, J. Poole, D.C. Wallace, J.C Lambert and V. Paquis-Flucklinger. (2008). Rapid
identification of mitochondrial DNA (mtDNA) mutations in neuromuscular disorders by using Surveyor strategy. Mitochondrion
Mar;8(2):136-45.
Eduardo Ruiz-Pesini, Marie T. Lott, Vincent Procaccio, Jason C. Poole, Marty C. Brandon, Pierre Baldi, and Douglas C. Wallace.
(2007) An enhanced MITOMAP with a global mtDNA mutational phylogeny. Nucleic Acids Res. Jan;35(Database issue):D823-8.
L.M. De La Maza, J.C.Poole, A.K.Sarcon, S. Pal, D. Li, V. Procaccio, D. Wallace. (2006) Dependence of Chlamydia on the host cell
mitochondrial DNA for growth and maturation. Proceedings of the Eleventh International Symposium on Human Chlamydial
Infections. Jun(11), 261-264.
Procaccio V, Neckelmann N, Paquis-Flucklinger V,Bannwarth S, Jimenez R, Davila A, Poole JC, Wallace DC. (2006). Detection of
Low Levels of the Mitochondrial tRNA(Leu(UUR)) 3243A>G Mutation in Blood Derived from Patients with Diabetes. Molecular
Diagnosis and Therapy. 10(6):381-9.
Jason C. Poole, Alison Thain, Neil D. Perkins and Igor B. Roninson. (2004). Induction of transcription by p21Waf1/Cip1/Sdi1: role
of NFkappaB and effect of non-steroidal anti-inflammatory drugs. Cell Cycle Jul;3(7):931-940.
David J. Gregory, Elisa Garcia-Wilson, Jason C. Poole, Andrew W. Snowden, Igor B. Roninson & Neil D. Perkins. (2002). Induction
of Transcription Through the p300 CRD1 Motif by p21WAF1/CIP1 Is Core Promoter Specific and Cyclin Dependent Kinase Independent.
Cell Cycle. 1:5, 343-350.
Chang, B.D., Watanabe, K., Broude, E.V., Fang, J., Poole, J.C., Kalinichenko, T.V., and Roninson, I.B. (2000). Effects of
p21WAF1/CIP1 on cellular gene expression: implications for carcinogenesis, senescence, and age-related diseases. Proc Natl Acad Sci
USA 97, 4291-6.
Chang, B.D., Broude, E.V., Fang, J., Kalinichenko, T.V., Abdryashitov, R., Poole, J.C., and Roninson, I.B. (2000). p21WAF1/CIP1-
induced growth arrest is associated with depletion of mitosis-control proteins and leads to abnormal mitosis and endoreduplication in
recovering cells. Oncogene 19, 2165-70.
Lin, J., Poole, J., and Linzer, D. I. (1997). Three new members of the mouse prolactin/growth hormone family are homologous to
proteins expressed in the rat. Endocrinology 138, 5541-9.

4. Lin, J., Poole, J., and Linzer, D. I. (1997). Two novel members of the prolactin/growth hormone family are expressed in the mouse
placenta. Endocrinology 138, 5535-40.
Sutton L.C., Lea S.E., Will M.J., Schwartz B.A., Hartley C.E., Poole J.C., Watkins L.R., Maier S.F. (1997). Inescapable shock-
induced potentiation of morphine analgesia. Behav Neuroscience 111, 1105-13.