Isolation and purification of Cyanobacteria... Introduction Procedure Mass cultivation by various methods Field application Production Crops response

1. Name: ANJALI PRIYDARSHNI
Class. :M.SC(BOT) Sem-ll
Roll No:- 02 Session:- 2022-24
Paper:- MBOTCC-5
Paper name:- Biofertilizer and technology
Department of Botany
College of Commerce, Arts & Science
ISOLATION AND PURIFICATION
OF CYANOBACTERIA

2. •***CONTENT ***
#CYANOBACTERIA
#ISOLATION OF CYANOBACTERIA
#MASS MULTIPLICATION OF
CYANOBACTERIA BY FOLLOWING
METHOD:-
• 1-TROUGH METHOD
2-FIELD METHOD
3-TANK METHOD
4-PIT METHOD
#PURIFICATION
#FIELD APPLICATION

3. # CYANOBACTERIA Introduction:-
•Prokaryotic cell
•1st photosynthetic bacteria existed on Earth
•Lack membrane bound organelle
•Consist of thylakoid –scattered inside the cell
•Cyanobacteria is blue blue green algae.
Chlorophyll (green)
Phycocyanin(blue)

4. • Tolerate wide range In environment i.e low to high temperature
Sustain in low salt conc.
#Diversity:-🌺non filamentous
🌺Filamentous without specialized cell e.g Oscillotoria
🌺Filamentous with specialized cell. e.g Anabaena

6. OUTLINE OF THE PROCEDURE to
isolate Cyanobacteria:-

10. # Mass multiplication of Cyanobacteria
🌱The Following four method are used For mass
multiplication of cyanobacteria:-
1.Tank method
2.Trough method
3.Pit method
4.Field method

12. Mass cultivation of cyanobacteria is done by
using any of the four methods under the
following steps:
a). Prepare the cemented tanks, shallow trays of iron sheets
or polythene lined pits in an open area. Width of tanks or
pits should not be more than 1.5m. This will facilitate proper
handling of culture.
B). Transfer 2-3 kg soil and add 100 g of superphosphate.
Water the pit to about 10cm height. Add 2ml of insecticide
e.g malathion to protect the culture from mosquitoes. Mix
well and allow to settle down soil particle.

13. C) when water become clear, sprinkle 100 g of
starter inoculum on the surface of water.
D) when temperature remains between 35-40°C
during summer, optimum growth of
cyanobacteria is achieved. Always maintain the
water level to about 10cm during this period.
E) After drying the algal mat will get separated
from the soil and forms flakes. During summer
about 1kg pure algal mat per m2 area is
produced. These are collected, powdered,
kept in sealed polythene bags and supplied to
the farmers.

14. #Purification of Cyanobacteria:-
🌹cyanobacteria can be purified by a variety of
techniques
such as centrifugation, filtration or
chromatography.
- These purified cultures can then be used for
research purposes or for industrial applications
such as biofuel production or bioremediation.

15. 🌱Field application of BGA
Inoculants
For one hectare of rice field, 10 Kg of
BGA inoculant is applied. However, after
10 days of transplantation Flakes is
dispersed which grows luxuriantly in
water.
🍃Crop response: –
-It has been found that BGA
incrases crop yield by 34% and
save nitrogen fertilizer by 30%