Media for general

1. S. No. Content
1. Plant Tissue Culture Media
2. Types of media
3. Media composition
4. Inorganic supplements
5. Organic supplements
6. Other supplements
7. Preparation of media
Sandhya Upadhyay

2. PLANT TISSUE CULTURE MEDIA
Media is a substance of supporting
and provide energy and food to the
developing explant.
They are of following type-
1. Solid and Liquid (Suspension
culture Media) depending in their
appearance.
2. Depending on their concentration
as well as constituents it can
varies by the type of explant and
species.

3. TYPES OF MEDIA ACCORDING TO THEIR
COMPOSITION
Murashige and Skoog used for plant regeneration and
organogenesis
White`s Medium used for root and ovule culture
B5 /Gamborg Medium is a type of cell suspension
used for callus culture
N6/ Chu for cereal anther culture
Nitsch`s used for anther culture

4. MEDIA COMPOSITION

5. INORGANIC SUPPLEMENTS- Macronutrients
Nitrogen
Potassium
Phosphorus
Calcium
Magnesium
Sulphur

6. • Calcium requires in cell wall formation and cell division further of it works as
enzyme and enzyme activator.
• Magnesium is a component of chlorophyll, enzyme activator as well as help in
energy utilization.
• Sulphur is a part of amino acids and important for respiration of plants.

7. INORGANIC SUPPLEMENTS -Micronutrient

8. Micronutrients

9. Organic Supplements
Vitamins
Amino
Acids
Energy
Sources
Activated
Charcoal
Antibiotics
Organic
Acids
Organic
Extract

10. Importance of Organic Supplements
Vitamins are used as enzyme and its
cofactors
Amino acids are the
constituents of proteins
Activated charcoal are used for
absorbing toxin and inhibitory
compounds secreted by plant
Sucrose is majorly required
as energy source
Organic acid helps in
regeneration and
development
Antibiotics are used for
maintaining
contaminated free
environments
Organic extract also
contain macro and
micronutrient but
in unknown
amount.

11. Others Constituents

12. Growth
Regulators
• Auxin
• Cytokinins
• Gibberellic Acid
• Abscisic Acid
Solidifying
Agents
• Agar
• Agarose
• Gel rite
• Clearagel
pH
• Range between 5.6
to 6
• Not lower than 4.5
and not higher than
7
Distil Water is used for mixing all the constituents

13. Preparation of Media
• Types of
concentration
• 10X
• 100X
Preparation
of stock
solution
• Addition of dry and wet
content separately
• Addition of gelling agent
after measuring pH
Mixing of
Constituents

15. STERILIZATION OF MEDIA