The document discusses different types of biofertilizers and their production. It describes biofertilizers as microbial inoculants that establish symbiotic relationships with plants to enrich soil nutrients and promote crop growth. Major biofertilizers include rhizobia, azotobacter, algae, and phosphate-solubilizing bacteria. Rhizobium and cyanobacteria (blue-green algae) are discussed in detail, outlining their role in nitrogen fixation and methods for mass production, including trough, pit and field methods.
he rhizosphere is the narrow region of soil or substrate that is directly influenced by root secretions and associated soil microorganisms known as the root microbiome.
The phyllosphere is a term used in microbiology to refer to the total above-ground portions of plants as habitat for microorganisms.
Mycorrhiza Biofertilizer is also known as VAM (Myco = Fungal + rrhiza = roots) adheres to plants rhizoids leading to development of hyphae. Hyphae boost development and spreading of white root in to soil leading to significant increase in rhizosphere. These hyphae further penetrate and form arbuscules within the root cortical. VAM fungi form a special symbiotic relationship with roots of plant that can enhance growth and survivability of colonized plants. Mycorrhiza Biofertilizer is very useful in organic farming as well as normal commercial farming
he rhizosphere is the narrow region of soil or substrate that is directly influenced by root secretions and associated soil microorganisms known as the root microbiome.
The phyllosphere is a term used in microbiology to refer to the total above-ground portions of plants as habitat for microorganisms.
Mycorrhiza Biofertilizer is also known as VAM (Myco = Fungal + rrhiza = roots) adheres to plants rhizoids leading to development of hyphae. Hyphae boost development and spreading of white root in to soil leading to significant increase in rhizosphere. These hyphae further penetrate and form arbuscules within the root cortical. VAM fungi form a special symbiotic relationship with roots of plant that can enhance growth and survivability of colonized plants. Mycorrhiza Biofertilizer is very useful in organic farming as well as normal commercial farming
It is a biofertilizer that contains symbiotic Rhizobium bacteria which is the most important nitrogen-fixing organism. These organisms have the ability to drive atmospheric Nitrogen and provide it to plants. It is recommended for crops such as Groundnut, Soybean, Red-gram, Green-gram, Black-gram, Lentil, Cowpea, Bengal-gram and Fodder legumes, etc.
Introduction :
Mycorrhizae are mutualistic symbiotic associations formed between the roots of higher plants and fungi.
Fungal roots were discovered by the German botanist A B Frank in the last century (1855) in forest trees such as pine.
In nature approximately 90% of plants are infected with mycorrhizae. 83% Dicots,79% Monocots and 100% Gymnosperms.
Convert insoluble form of phosphorous in soil into soluble form.
It is a biofertilizer that contains symbiotic Rhizobium bacteria which is the most important nitrogen-fixing organism. These organisms have the ability to drive atmospheric Nitrogen and provide it to plants. It is recommended for crops such as Groundnut, Soybean, Red-gram, Green-gram, Black-gram, Lentil, Cowpea, Bengal-gram and Fodder legumes, etc.
Introduction :
Mycorrhizae are mutualistic symbiotic associations formed between the roots of higher plants and fungi.
Fungal roots were discovered by the German botanist A B Frank in the last century (1855) in forest trees such as pine.
In nature approximately 90% of plants are infected with mycorrhizae. 83% Dicots,79% Monocots and 100% Gymnosperms.
Convert insoluble form of phosphorous in soil into soluble form.
CONTENT
CONTENT
CONTENT
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Biofertilizer
Role of biofertilize
Types of biofertilizer
Azotobacter
Characteristics
Nitrogen fixation
Mode of action of Azotobacter
Isolation of Azotobacter
Mass production of Azotobacter
Mixing and packaging
Ideal characteristics of carrier
material
Preparation of inoculum packet
Application
Advantage and disadvantage
Reference
Till 1997-98 strong correlation is found between Fertilizer consumption and food grains production
After 1997-98, this relationship distorted
Most of States are experiencing increase in fertilizer consumption with slower pace of crop productivity
Some states witness consumption of fertilizer picking up without any conspicuous gain on agricultural crop productivity
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
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Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
2. Biofertilizers are the compounds that enrich the nutrient
quality of the soil by using microorganisms that establish
symbiotic relationships with the plants
These are the microbial inoculants which are artificially
multiplied cultures of certain soil microorganisms that can
improve soil fertility and crop productivity
Biofertilizers add nutrients through their activities like
nitrogen fixation, phosphorus solubilization and
stimulating plant growth through the synthesis of growth
promoting substances.
3. Plant growth promoting microorganisms
include bacteria such as Azospirillum spp.,
Pseudomonas spp., Bacillus spp. while fungus
include Trichoderma spp.
Biofertilizers include organic fertilizers which
are rendered in an available form due to
interactions of microorganisms or their
association with plants majorly Rhizobium spp.
4.
5. Majorly, biofertilizers include the following
types:
1. Rhizobium spp. as symbiotic nitrogen fixers
2. Azospirillum spp. and Azotobacter spp. as
asymbiotic nitrogen fixers
3. Algae biofertilizers
4. Phosphate solubilizing bacteria
5. Mycorrhizae
6.
7. Rhizobium is a Gram negative bacterium which
inhabits the root nodules of most of the leguminous
plants
Rhizobia are soil inhabiting bacteria that fix
nitrogen after becoming established inside the root
nodules
Rhizobia donot produce spores and are aerobic and
motile too
Rhizobia maintain symbiotic relationships with
legumes by responding chemotactically to flavonoid
molecules released as signals by the legume host
plant.
8. There are some steps involved in mass production of Rhizobium to use
them as biofertilizers. These are as follows:
Preparation of mother or starter culture
Preparation of broth culture
Preparation of carrier
Preparation of inoculate (Mixing)
Maturation
Filling and packaging
Quality checking
Storage
9.
10.
11. Cyanobacteria which are also known as blue-green
algae, are photoautotrophic and prokaryotic in nature
They are free living and fix the atmospheric nitrogen in
moist soils
They also include unicellular as well as filamentous
species having specialized cells known as heterocysts
such as Anabaena and Nostoc
These cells are the site for nitrogen fixation and few of
those which are non heterocystous can reduce N2 into
NH3 i.e nitrogen fixation.
12.
13.
14. The mass production of BGA can be processed
in the following ways mainly:
1. Trough method
2. Pit method
3. Field method
15. This method is basically used in laboratory where zinc and
iron troughs are used
These are dimensionally 2 x 3 cm in width and 22 cm in
height
Trough is filled with about 10kg of soil and 200g of
superphosphate is spread on it
Water is poured upto 5-10cm height and calcium carbonate
is added to adjust pH around 7
Then, starter culture is sprinkled over it
Trough is kept in sunlight where BGA is developed
Watered everyday
After sufficient growth of BGA, soil is allowed to dry and
the dry flakes are collected and packed for algalization.
16. In this method, under full sunlight, shallow pits
are maintained
To avoid percolation, polythene sheets are lined
inside the pit
Soil is filled in pit upto 20 cm and watered for 10
cm height
Then, carbofuran and saw dust are added along
with the starter culture sprinkling
Then, similarly after the growth of BGA, the dry
flakes are collected and packed.
17. This method requires an open and maintained
field of about 40 sq.mts
The plot is watered upto 15 cm height and 20kg
superphosphate is added to it
After maintaining pH around 7, carbofuran is
added
Then, the starter culture is added about 5kg and is
frequently watered
In about 3-4 weeks, BGA developed and soil is
allowed to dry
In this method, about 30kg of BGA can be
harvested.
18. NOTE:
About 10 kg of blue green algae inoculants is
recommended for one hectare of flooded
rice. The dried BGA flakes are added after
10 days of implantation. The application of
BGA is known as algalization. It is reported
that BGA increases the yield upto 34% in rice
fields.
19.
20. Seed treatment: Depending on the seed rate, the required
quantity of jaggery is boiled in water and cooled. Rhizobium
inoculum is mixed in the jaggery solution and sprinkled over
the seeds followed by mixing of seeds with inoculum over the
entire surface of seeds. Seeds are dried under shade and sown
immediately
Soil treatment: The rhizobium inoculum is mixed with soil
and spread over the field
Soil Application: If Rhizobium is not available, then, as an
alternative 200kg of surface soil can be collected from the field
where that particular leguminous crop was grown in abundance
and then can be applicated in the field where the crop is to be
sown for the first time.