The document discusses primary and secondary metabolites, alkaloids, glycosides, and tannins. It defines these compounds and outlines their key properties. Alkaloids are nitrogen-containing compounds that have physiological effects. They are classified based on biosynthetic pathways, pharmacology, taxonomy, and heterocyclic ring structures. Glycosides are compounds containing a sugar and non-sugar moiety. They are classified by the type of sugar or aglycone. Tannins are polyphenolic compounds that can combine with proteins. They are classified as hydrolysable or condensed based on their chemical structures. Extraction and isolation methods are also presented.
Secondary metabolites from plants like Alkaloids, Glycosides, Tannins, Resins, Volatile oils and Flavonoids. with definition, introduction, Chemical properties, Classification and Identification test
1) BASED ON THE CHEMICAL NATURE OF NON SUGAR MOIETY
2) BASED ON TE NATURE OF SUGAR MOITY
3) BASED ON LINKAGE BETWEEN GLYCON AND AGLYCON PORTION
4) BASED ON THERAPEUTIC NATURE OF GLYCOSIDE
Commonly known as its anionic form shikimate, is a cyclohexene, a cyclitol and a cyclohexanecarboxylic acid.
It is an important biochemical metabolite in plants and microorganisms.
Its name comes from the Japanese flower shikimi the Japanese star anise, Illicium anisatum), from which it was first isolated in 1885 by Johan Fredrik Eykman.
The elucidation of its structure was made nearly 50 years later.
Shikimic acid is also the glycoside part of some hydrolysable tannins.
The shikimate pathway is a seven step metabolic route used by bacteria, fungi, algae, parasites, and plants for the biosynthesis of aromatic amino acids (phenylalanine, tyrosine, and tryptophan).
This pathway is not found in animals; therefore, phenylalanine and tryptophan represent essential amino acids that must be obtained from the animal's diet
Animals can synthesize tyrosine from phenylalanine, and therefore is not an essential amino acid except for individuals unable to hydroxylate phenylalanine to tyrosine).
Secondary metabolites from plants like Alkaloids, Glycosides, Tannins, Resins, Volatile oils and Flavonoids. with definition, introduction, Chemical properties, Classification and Identification test
1) BASED ON THE CHEMICAL NATURE OF NON SUGAR MOIETY
2) BASED ON TE NATURE OF SUGAR MOITY
3) BASED ON LINKAGE BETWEEN GLYCON AND AGLYCON PORTION
4) BASED ON THERAPEUTIC NATURE OF GLYCOSIDE
Commonly known as its anionic form shikimate, is a cyclohexene, a cyclitol and a cyclohexanecarboxylic acid.
It is an important biochemical metabolite in plants and microorganisms.
Its name comes from the Japanese flower shikimi the Japanese star anise, Illicium anisatum), from which it was first isolated in 1885 by Johan Fredrik Eykman.
The elucidation of its structure was made nearly 50 years later.
Shikimic acid is also the glycoside part of some hydrolysable tannins.
The shikimate pathway is a seven step metabolic route used by bacteria, fungi, algae, parasites, and plants for the biosynthesis of aromatic amino acids (phenylalanine, tyrosine, and tryptophan).
This pathway is not found in animals; therefore, phenylalanine and tryptophan represent essential amino acids that must be obtained from the animal's diet
Animals can synthesize tyrosine from phenylalanine, and therefore is not an essential amino acid except for individuals unable to hydroxylate phenylalanine to tyrosine).
Crude drugs are plant, animal or their parts which after collection are subjected only to drying or making them into transverse/ longitudinal slices pieces or peeling them in some cases. They exist in natural form.
Crude drugs may be derived from various natural sources like Plants, Animals, Minerals, Marine and Micro-organisms etc
Flavonoids classification, isolation and identificationMona Ismail
Flavonoids are groups of polyphenolic compounds which are found in fruits, flowers, seeds & vegetable.
(named from the Latin word flavus meaning yellow, their colour in nature)
Occurrence and classification and function of alkaloidsJasmineJuliet
Alkaloids introduction, Alkaloids classification, Alkaloids function, pharmaceutical applications of alkaloids, Examples of alkaloids, Some review questions related to alkaloids.
Crude drugs are plant, animal or their parts which after collection are subjected only to drying or making them into transverse/ longitudinal slices pieces or peeling them in some cases. They exist in natural form.
Crude drugs may be derived from various natural sources like Plants, Animals, Minerals, Marine and Micro-organisms etc
Flavonoids classification, isolation and identificationMona Ismail
Flavonoids are groups of polyphenolic compounds which are found in fruits, flowers, seeds & vegetable.
(named from the Latin word flavus meaning yellow, their colour in nature)
Occurrence and classification and function of alkaloidsJasmineJuliet
Alkaloids introduction, Alkaloids classification, Alkaloids function, pharmaceutical applications of alkaloids, Examples of alkaloids, Some review questions related to alkaloids.
Alkaloids: Introduction, Property and classification OP VERMA
Alkaloids are major secondary metabolites reported in higher plants. In this PPT I have covered Introduction, Property, Clarification and identification tests.
This ppt contains a suitable contents. This notes is effective for B.Pharm & D.Pharm students. In this notes we all the related topic of secondary metabolites. In this notes we all sub topics of secondary metabolites such as Alkaloids, Glycosides, Terpenoids, Taninns, Resins, Flavonoids, etc.
Alkaloids are nitrogenous compounds of low molecular weight. They are mainly produced by plants and animals for defense. Examples of alkaloids include morphine, codeine, coniine, quinine, scopolamine, hyoscamine, atropine, caffeine, sangunarine, berberine, etc.
anthraquinone, coumarin, cyanogens (cyanohydrin), flavonoids, glucosinolates (or thioglycosides), phenols, steroidal, terpenoids, and saponins.
A type of chemical found in plants and in certain foods, such as fruits, vegetables, nuts, wine, and tea.
This presentation is all about Alkaloids which are organic compounds. Their occurence, where they found in nature. Various physical and chemical properties of alkaloids. Why they are basic in nature, How they are classified and several tests for alkaloids. The extraction process used for alkaloids extraction. Finally few alkaloids with their medicinal uses,structures and few hetreocyclic compounds present in alkaloids.
Pharmaceutical Inorganic chemistry UNIT-V Radiopharmaceutical.pptx
Isotopes Types of decay
Alpha rays, which could barely penetrate a piece of paper
Beta rays, which could penetrate 3 mm of aluminium
Gamma rays, which could penetrate several centimetres of lead
Units of Radioactivity:
Measurement of Radioactivity
The measurement of nuclear radiation and detection is an important aspect in the identification of type of radiations (, , ) and to assay the radionuclide emitting the radiation, suitable detectors are required. The radiations are identified on the basis of their properties.
e.g. Ionization effect is measured in Ionization Chamber, Proportional Counter and Geiger Muller Counter.
The scintillation effect of radiation is measured using scintillation detector and the photographic effect is measured by Autoradiography.
Gas Filled Detectors:
Ionization Chamber:
Proportional Counters:
Geiger-Muller Counter
Properties of α, β, γ radiations
Half –life of Radioelement
Sodium Iodide (I131)
Handling and Storage of Radioactive Material:
Storage of Radioactive Substances –
Precautions For Handling Radioactive Substances
Labelling of Radioactive Substances
Pharmaceutical Application Of Radioactive Substances
Major extra and intracellular electrolytes. Pharmaceutical Inorganic chemistr...Ms. Pooja Bhandare
Major extra and intracellular electrolytes. Pharmaceutical Inorganic chemistry UNIT-II (Part-II)
Electrolyte: Intracellular fluid
Interstitial fluid
Plasma (Vascular fluid)
Anionic electrolytes- HCO₃⁻, Cl⁻, SO₄²⁻, HPO₄²⁻
Cationic electrolytes- Na⁺, K⁺, Ca²⁺, Mg²⁺
Concentration of important Electrolytes:
Electrolytes used in the replacement therapy: Sodium
chloride*, Potassium chloride, Calcium gluconate* and Oral Rehydration Salt
(ORS), Physiological acid base balance.
Acids, Bases And Buffers Pharmaceutical Inorganic chemistry UNIT-II (Part-I)
Acids, Bases are defined by Four main theories,
1.Traditional theory / concept
2.Arrhenius theory
3.Bronsted and Lowry theory
4.Lewis theory
Importance of acids and bases in pharmacy
Buffers: Buffer action
Buffer capacity Buffers system
Types of Buffers : Generally buffers are of two types:
1. Acidic buffers
2. Basic buffers
There are some other buffer system:
3. Two salts acts as acid-base pair. Ex- Potassium hydrogen phosphate and potassium dihydrogen phosphate.
4. Amphoteric electrolyte. Ex- Solution of glycine.
5. Solution of strong acid and solution of strong base. Ex- Strong HCl with KCl Mechanism of Buffer action: Mechanism of Action of acidic buffers: Buffer equation-Henderson-Hasselbalch equation:
Standard Buffer Solutions Preparation of Buffer Solutions: Buffers in pharmaceutical systems or Application of buffer: Stability of buffers Buffered isotonic solution Types of Buffer Isotonic solution
1. Isotonic Solutions:
2. Hypertonic Solutions:
3. Hypotonic Solution:
Measurement of Tonicity: 1. Hemolytic method: 2. Cryoscopic method or depression of freezing point:
Methods of adjusting the tonicity:
Class I methods:
In this type, sodium chloride or other substances are added to the solution in sufficient quantity to make it isotonic. Then the preparation is brought to its final volume withan isotonic or a buffered isotonic diluting solution.
These methods are of two types:
Cryoscopic method
Sodium chloride equivalent method.
Class II methods:
In this type, water is added in sufficient quantity make the preparation isotonic. Then the preparation is brought to its volume with an isotonic or a buffered isotonic diluting solution.
These methods are of two types:
White-Vincent method
Sprowls method.
Limt test Pharmaceutical Inorganic chemistry UNIT-I (Part-III) Limit Test.
Limit tests:- Factors affecting limit tests:
Specificity of the tests
Sensitivity
Control of personal errors (Analyst errors)
Test in which there is no visible reaction
Comparison methods
Quantitative determination
Limit test for Chloride: Principle, Procedure, observation and result.
Limit test for Sulphate: Principle, Procedure, observation and result
Limit test for Iron: Principle, Procedure, observation and result.
Limit test for Heavy metal: Principle, Procedure, observation and result.
Limit test for Lead: Principle, Procedure, observation and result.
Limit test for Arsenic: Principle, Gutzet test Procedure, detail in Gutzet Apparatus. observation and result.
Modifies Limit test for Chloride: Principle, Procedure, observation and result.
Modified Limit test for sulphate: Principle, Procedure, observation and result.
Types and Sources of impurities.pptx Pharmaceutical Inorganic chemistry UNIT-...Ms. Pooja Bhandare
Types and Sources of impurities. Pharmaceutical Inorganic chemistry UNIT-I (Part-II) Impurities:
Impure Chemical Compound
Pure Chemical Compound.
Types of impurities: Organic Impurity, Inorganic impurity, Residual solvent, Sources of Impurities in Pharmaceuticals
The different sources of impurities in pharmaceuticals are listed below:
Raw material used in manufacture
Reagents used in manufacturing process
Method/ process used in manufacture or method of manufacturing
Chemical processes used in the manufacture
Atmospheric contamination during the manufacturing process
Intermediate products in the manufacturing process
Defects in the manufacturing process
Manufacturing hazards
Inadequate Storage conditions
Decomposition of the product during storage
Accidental substitution or deliberate adulteration with spurious or useless materials.
Test for purity: Pharmacopoeia prescribes the “Test for purity” for pharmaceutical substances to check their freedom from undesirable impurities.
Pharmacopoeia will decide and fix the limit of tolerance for these impurities.
For certain common impurities for which pharmacopoeia prescribes the test of purity are:
Colour, odour, taste
Physicochemical constants (Iodine value, saponification value, melting point, refractive index etc.)
Acidity, alkalinity, pH
Humidity (Estimation of moisture)
Cations and anions
Insoluble Constituent or Residue.
Ash, Water insoluble ash
Arsenic or lead
Loss on drying
Loss on ignition.
Effect of Impurities
Introduction of Inorganic Chemistry, History of Pharmacopoeia.pptxMs. Pooja Bhandare
Introduction of Inorganic Chemistry, History of Pharmacopoeia, Pharmaceutical Chemistry, Inorganic Chemistry:
IMPORTANTS OF INORGANIC CHEMISTRY, Introduction of Pharmacopoeia, Types of Pharmacopoeia, History of pharmacopoeia, HISTROY OF INDIAN PHARMACOPOEIA
Content of pharmacopoeia Introduction including general Notices
Monographs of the official drugs
Appendices
Polyploidy, mutation and hybridization with reference to medicinal plants. PH...Ms. Pooja Bhandare
Polyploidy, mutation and hybridization with reference to medicinal plants. PHARMACOGNOSY & Phytochemistry-I (BP405T)Unit-IIPart-4
Polyploidy reference to medicinal plants.
Types Of Polyploidy
A. Euploidy
a.Autopolyploidy
b. Allopolyploidy
B. Aneuploidy
1. Causes Of Polyploidy
2. Non-disjunction in mitosis
3. Non-reduction in meiosis
4. Polyspermy
5. Endo-replication or Endo- reduplication.
Factors Promoting Polyploidy
1. Physical factor
2. Chemical factor
3. Biological factor
Physical factor:-
Temperature :- heat temperature & cold temperature
Centrifugation
X-rays
Gamma rays
Cosmic rays
Ionizing & non-ionizing radiations
UV-radiations
Chemical factor:-
Alkylating agents:- nitrogen & sulphur mustard
Acridines
Proflavins
Nitrous acid
Colchicines[6]
Colchicines (Poisonous alkaloids):-
Biological factor
Mode of reproduction
Mode of fertilization
Breeding system present (Hybridization)
Growth habit of the plant
Size of chromosomes
Application Of Polyploidy
Mutation breeding
Seedless fruits production
Bridge crossing
Ornamental & forage breeding
Disease resistance through aneuploidy
Industrial application of polyploidy
mutation reference to medicinal plants
Type of mutations:
1. Spontaneous and induced mutations.
2. Recessive and dominant mutations.
3. Somatic and germinal mutations.
4. Forward, back and suppressor mutation.
5. Chromosomal, genomic and point mutations
Application Of Mutation:
Hybridization reference to medicinal plants
The following steps are involved in hybridization of plant:
Choice Of Parents:.
Selfing Of Parents
Emasculation:.
Bagging:
Crossing Or Cross Pollination
Labelling
Collection Of Hybrid Seeds
Significance of Hybridization
PHARMACOGNOSY & Phytochemistry-I (BP405T)Unit-IIPart-2.FACTORS AFFECTING CULTIVATION
1. Altitude
2.Temperature
3. Rainfall
4. Day Length and Day Light
5. Soil
6. Soil Fertility
7. Fertilizers and Manures
a) Chemical fertilizers
(b) Manures
(c) Biofertilizers
8. Pests and Pests Control
a. Microbes
b) Insects
C) Non insect pests
d) Weeds
9. Other Factors that Affect the Cultivated Plants
a. Air Pollution
b. Herbicide
Cultivation and collections of drugs of natural origin..pptxMs. Pooja Bhandare
PHARMACOGNOSY & Phytochemistry-I (BP405T)Unit-IIPart-1Cultivation and collections of drugs of natural origin.
Advantages of cultivation
Methods of Plant Propagation
1.Sexual method (seed propagation)
2. Asexual method
Methods of sowing the seeds
Broadcasting Dibbling Miscellaneous
Special treatment to seeds
Asexual method.
Asexual method of vegetative propagation consists of three types:
a) Natural methods of vegetative propagation.
b) Artificial methods of vegetative propagation.
c) Aseptic method of micropropagation (tissue-culture).
COLLECTION OF CRUDE DRUGS
HARVESTING OF CRUDE DRUGS
DRYING OF CRUDE DRUGS
(1) natural (sun drying) and (2) artificial
Artificial Drying
Drying by artificial means includes drying the drugs in
(a) an oven; i.e. tray-dryers;
(b) vacuum dryers and
(c) spray dryers.
GARBLING (DRESSING)
PACKING OF CRUDE DRUGS
STORAGE & PRESEVATION OF CRUDE DRUGS
Quality control of Drugs of Natural Origin. PHARMACognosy & Phytochemistry-I ...Ms. Pooja Bhandare
Quality control of Drugs of Natural Origin PHARMACognosy & Phytochemistry-I (BP405T)Unit-I Part-3.
CONTENTS
Adulteration
Evaluation of adulteration
Morphological / Organoleptic evaluation
Microscopic evaluation
Quantitative evaluation
Physical evaluation
Chemical evaluation
Biological evaluation
Adulteration is of two types:
Indirect or Unintentional adulteration
Direct or Intentional adulteration
Intentional adulteration may be due to the following reasons
adulteration using manufactured substances
substitution using inferior commercial varieties
substitution using exhausted drugs
substitution of superficially similar inferior natural substance
adulteration using the vegetative part of the same plant
addition of toxic materials
adulteration of powders
addition of synthetic principles
Evaluation of Crude Drugs
1. ORGANOLEPTIC EVALUATION
2. MICROSCOPICAL EVALUATION
Stomatal index Vein-islet number
Veinlet termination number
Palisade ratio
Quantitative Microscopy (Lycopodium Spore Method)
3.CHEMICAL EVALUATION
4. Physical Evaluation
I. Solubility
II. Optical Rotation
III. Refractive Index
III. Specific Gravity
IV Viscosity
V. Melting Point
VI. Moisture Content
VII. Ultraviolet Light
VIII. Ash Values
Total ash
Acid-insoluble ash
The water-soluble ash
IX. Extractive Values
X. Foreign Organic Matters
5. BIOLOGICAL EVALUATION
Toxicity
Oxytocic activity
Microbiological assays
Classification of Crude Drugs. HARMACognosy & Phytochemistry-I (BP405T)Unit-I...Ms. Pooja Bhandare
Classification of Crude Drugs.PHARMACognosy & Phytochemistry-I (BP405T)Unit-I Part-2.
A method of classification should be:
a) simple,
b) easy to use, and
c) free from confusion and ambiguities.
TYPES OF CLASSIFICATION.
1.Alphabetical classification
2.Taxonomical classification
3.Morphological classification
4.Pharmacological classification
5.Chemical classification
6.Chemotaxonomical classification
7. Serotaxanomical Classification
Animal Cell Culture: Growth of animal cells in culture. PHARMACEUTICAL MICROB...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-VPart-4
Animal Cell Culture: Growth of animal cells in culture.
Introduction: Histroy, The culture media used for animal cell culture are classified as,
Natural, Artificial, Synthesized
Natural Culture Media:
a. Blood Plasma:
b. Blood Serum:
c. Tissue Extracts:
Artificial Media
Some common examples of artificial media are,
Minimal Essential Medium (MEM),
CMRL 1066,
RPMI 1640.
Synthetic media re classified as,
Serum Containing Media.
Serum Free Media.
a. Serum Containing Media:
b. Serum Free Media:
Physicochemical Parameters needed for growth animal cell culture:
General procedure for cell Culture.
Isolation of the tissue:
Disaggregation of the Tissue:
Mechanical disaggregation
b. Enzymatic Disaggregation
. Trypsin based disaggregation or trypsinization:
Warm trypsinization:
Cold trypsinization:
Drawbacks of trypsin disaggregation:
B. Collagenase based disaggregation:
C. Chelating Agents:
3. Seeding of Culture:
Preservation of pharmaceutical products using antimicrobial agents. PHARMACEU...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-VPart-3
Preservation of pharmaceutical products using antimicrobial agents.
Introduction. Ideal Properties of Preservatives:
Antimicrobial Chemical Preservatives
Development of a Preservative System.
Factors affecting efficacy of a preservative: 1. Interaction With components of the formulation
2. Properties of the Preservatives:
3) Effect of Containers.
4) Type of microbes:
5) Influence of pH:
Challenge Test: Efficacy Test of Preservative : Medium used, Choice of test organism:
Preparation of the inoculum:
Procedure:
Interpretation of Results:
Assessment of microbial contamination and spoilage. PHARMACEUTICAL MICROBIOLO...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-VPart-2
Assessment of microbial contamination and spoilage.
Assessment of microbial contamination and spoilage
1. Physical and chemical changes:
2. Assessment of viable microorganisms in non-sterile products:
3. Sterility test:
4. Estimation of pyrogens:
Microbial Limit Tests:
Total Aerobic Microbial Count:
Membrane Filtration.
Plate Count Methods.
Pour Plate Method.
Surface spread Method.
Most Probable Number(MPN)
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
How to Split Bills in the Odoo 17 POS ModuleCeline George
Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Ethnobotany and Ethnopharmacology:
Ethnobotany in herbal drug evaluation,
Impact of Ethnobotany in traditional medicine,
New development in herbals,
Bio-prospecting tools for drug discovery,
Role of Ethnopharmacology in drug evaluation,
Reverse Pharmacology.
2. PRIMARY AND SECONDARY METABOLITE
PRIMARY METABOLITE
1. Widely distriuted in nature practically in all
oraganism
2. Directly involved in growth, development
and reproduction.
2. They are not poisonous.
3. Example Carbohydrate, lipids, proteins and
amino acid.
SECONDARY METABOLITE
1. Biosynthesized derived from primary
metabolites more limited distributed in
taxanomic plant.
eg. Reserpine from Rauwolfia serpentine
2. They do not directly involved in the normal
growth, development and reproduction
3. Some of these compounds are poisonous
4. Example Alkaloids, Glycosides, tannins,
Volatile oil , Resins and Resins
combination
3. Alkaloids
Coined by W. Meissner German Pharmacist in 1819.
The term derived from the word “alkali like” so they have some character similar to
naturally occurring complex amines.
Defination
Alkaloids are the organic products of plant origin, basic in nature and contain one or more
nitrogenous atoms normally of heterocyclic in nature, and have marked physiological
action when administered internally
4. GENERAL PROPERTIES OF THE ALKALOIDS
PHYSICAL PROPERTIES
Colourless, Crystalline solid and posses a sharp melting point
Some alkaloids like nicotine and connine are liquid and volatile in nature.
Some alkaloids are coloured like berberine is yellow and bentanidin is red.
Solubility: Free alkaloids bases are fairly soluble in O S, Non polar solvent and lower alcohol and practically
insoluble OR sparingly soluble in water.
salt alkaloids are freely soluble in water, less soluble in alcohol and very springily soluble in organic solvent.
5. CHEMICAL PROPERTIES
The normal elements present in the alkaloids are carbon, hydrogen and oxygen
but every alkaloid should essential contain at least one nitrogen imparts for basic
properties.
Chemical Test
1. Mayer’s reagents: Potassium – Mercuric iodide solution gives cream coloured
precipitate
2. Wagner’s reagents: Potassium Tri iodide solution gives reddish brown
precipitate.
3. Dragendorff’s reagent: Potassium bismuth iodide solution gives reddish brown
precipitate or Orange red precipitate.
4. Hager’s reagent: Saturated solution of picric acid gives Yellow coloured
precipitate.
6. CLASSIFICATION OF ALKALOIDS
Biosynthetic Classification:
Pharmacological Classification
Taxonomic Classification
Chemical Classification
7. Biosynthetic classification
Given to the Precursor from which the alkaloids are produced in plant biosynthetically.
So all the alkaloids derived from the same precursor can be brought under same group even they have
different taxonomic distribution and pharmacological activity.
e. g. piperidine alkaloids derived from lysin, pyrrolidin alkaloids derived from ornithin and indole derived
from tryptophan.
Pharmacological Classification
Classification depends upon Pharmacological action e.g. CNS stimulant or depression and anti malarias etc
Individual alkaloids may exhibit different action within the same drug for e.g. in cinchona, quinine is an anti
malarial where as quinidine is a cardiac depressant. In opium morphine is narcotic analgesic where as codine
is antitussive.
8. Taxonomic classification:
Classification deals with the ‘Taxon’
Like genus, subgenus, species and subspecies etc
Large number of alkaloids are classified on their like various family like rubiaceous
alkaloids and solanceous alkaloids.
Some phytochemist have stepped further and classified alkaloids based on
chemotaxonomic classification
9. Chemical Classification
Widely accepted classification of alkaloids.
The basic of classification is the ring structure (normally heterocyclic ring) present in the alkaloids.
Divided into two categories.
1. Non Heterocyclic or Proto alkaloids.
2. Heterocyclic or typical alkaloids.
Large number of alkaloids which posse heterocyclic ring structure are mentioned below
Type Example
1. Pyrrolidine Hygrine Stachydrine
2. Pyridine Ricinine, Arecoline
3. Piperdine Lobeline, Connine
4. Tropane Atropine, Cocaine
5. Quinoline Cinchonine, Quinine, Qunidine
6. Isoquinoline Papaverine, Morphine,Emetine
10. GENERAL METHODS OF EXTRACTION AND
ISOLATION OF ALKALOIDS
Methods 1 :-
Drug (Powdered) Moisten with H2O extracted with organic solvent Filtrate
&treated with lime (Petroleum spirit or ether)
Add water Separate Shaken with aqueous Reject the organic layer Aqueous layer
Organic layer acid(allow to separate) (Contains the
alkaloids salts.
Filter
11. Methods 2:-
Drug (Powdered) Moisten with H2O extracted with alcohol Filtrate
&treated with acid or water
Add acetone Reject the organic layer Aqueous layer ammonia or sodium
(Contains several impurities) (contains alkaloids) bicarbonate
Add organic Reject the aqueous layer Organic layer contains
Solvent alkaloids
Filter
Treated with
12. FUNCTIONS OF ALKALOIDS IN PLANT
They may have a vital role in growth regulatory factor.
The alkaloids are poisonous in nature thus they protect the plant grazing
animals or insects
They act as a reserve substance in plant and supply nitrogen or other
elements.
They might be the by-product of various detoxification reaction in plants
and by this way they cease the formation of harmful substance in plant.
They are present in association with plant acid like quinic acid,
cinchotannic acid etc. Hence they may provide the means of storing or
transportation of such acids.
13. GLYCOSIDES
Defination
The organic compounds mainly of plant origin and rarely of animal which
on enzymatic or acid hydrolysis yields one or more sugar
moieties(Glycon) and a non sugar moieties (Aglycon or Genin).
The sugar (glycon) present in glycosides are monosaccharides like
glucose, rhamnose and rarely deoxysugars such cymarose found in cardiac
glycosides.
The aglycon part may be alcohol, phenol or amines.
The linkage between glycon and and aglycon is know as glycosidic
linkage.
14. Properties
Colourless but some them are coloured like flavonoids are yellow and anthracene
glycosides are red.
They are crystalline or amorphous solid compounds.
Glycosides are optically active and normally levo form is more active
Soluble in water and alcohol but insoluble insoluble in chloroform and
ether.
Glycosides can be hydrolysed by mineral acid, water and enzymes.
15. ISOLATION-(STAS-OTTO METHOD)
Powder crude drug
Extracted with alcohol and
filter it
Residue Filtrate
(Tannis+Impurities+Glycosides)
Add lead acetate solution
(Excess of lead acetate is precipitated
by passing hydrogen sulphide gas)
Filter it
Tannin and Impurity Filtrate
Fractional crystallization
Fraction Solubility Chromatography
Pure glycoside
16. Classification
1. On the basis of the type of the sugar or the glycone part e.g. glucoside with glucose, fructoside with
fructose and pentosides with pentose etc.
2. Glycosides are classified on the basis of the pharmacological action exhibited by them For e,g
Purgative glycosides – Aloe, Senna
Cardiac glycosides – Digitalis, Thevetia
Diuretics – Gokhru
3. Glycosides are also classified on the basis of linkage between glycone and aglycone part
A. O- glycosides- In these glycosides sugar is connected to OH or phenol group
-OH + HO-C6H11O5 -O-C6H11O5 + H2O
Example- Rhubarb and Senna etc
B. C- glycosides – In this type of glycosides sugar is connected to carbon atom
CH + HO-C6H11O5 -C-C6H11O5 + H2O
Example- Aloe and Cascara
17. C. S- glycosides – In this type of glycosides sugar is connected to sulphur of SH group
-SH + HO-C6H11O5 S-C6H11O5 + H2O
Example- Sinigrin from black mustard
D. N- glycosides – In this type of glycosides sugar is connected to nitrogen of NH group
N-H + HO-C6H11O5 N-C6H11O5 + H2O
Example- Nucleosides
4. Glycosides classified on the basis chemical nature
1) Anthracene glycoside
2) Saponin glycosides
3) Cardiac glycosides
4) Cyanogenetic glycosides
5) Isothiocynate glycosides
6) Coumarin Glycosides
7) Aldehyde glycosides
8) Steriodal glycoside
9) Phenol glycosides
10) Flavonoid glycosides
11) Bitter and Miscellaneous glycosides
18. TANNINS
INTRODUCTION:-
Tannins are polyphenolic substances found in many plants product of secondary metabolites. As
the name indicate they posses the property of tan i.e to convert hide and skin into the leather
Detected by qualitatively by tannins test i.e the Goldbeater’s skin test
Present in cell sap and vacuoles.
They act as astringent they have capacity to combine with protein and precipitate them.
DEFINATION:-
The complex, organic, non-nitrogenous, phenolic substance of higher molecular weight.
• They are used as antiseptics and in gastro-intestinal disease like diarrhoea. They are also
used in leather indust
19. • PROPERTIES
Tannins are soluble in water, alcohol, dil alkalies, glycerine and acetone but insoluble in
organic solvent such as benzene ether and chloroform,
They should posses tanning properties
Tannins with ferric salt gives blue, violet or green colour.
They have precipitate with alkaloids and have heavy metals therefore they are used as
antidotes in alkaloidal and heavy metal poisoning.
In aqueous solution tannin produce acidic reaction and have astringent test.
20. • CLASSIFICATION
Classification based on chemical nature
1. Hydrolysable tannins
2. Condensed tannins
1. Hydrolysable tannins:-
These tannins are hydrolysed by acids or enzymes and produce gallic acid or ellagic acid
Chemically they are esters of sugar usually glucose with one or more trihydroxybenzene
carboxylic acid.
With ferric chloride they produce they produce blue colour hence they are used in
manufactured in ink.
Example:- gallotannin from rhubarb, chestnut, nutgall and clove and ellagitannin from
myrobalam of oak
21. 2. Condensed tannins
They are also called as phlobatannins or proanthocyanidins.
They are related to flavonoids pigments and have the polymeric formula flavone-3-ol.
On the treatment with acids or enzymes these tannins are decomposed into red insoluble
compounds called as phlobaphens.
With ferric chloride they produce green colours.
On heating these tannins produce catechol, therefore they are also called as catechol tannins.
The drugs which contain condensed tannins are cinchona bark, cinnamon bark, pale and black
catechu, cocoa, kola seeds etc.
22. Pseudotannins-
Pseudotannins are low molecular weight compounds and do not respond to Goldbeater’s skin
test.
Example of pseudotannins are catechins from cocoa and chlorogenic acid from nuxvomica and
coffee.
23. Extraction and isolation of tannins.
The various type of the methods of extraction depending the source of tannins are employed.
As the tannins are high molecular weight compounds so it becomes difficult to isolate the
tannins in pure form.
Thus the solvent used are the mixture of polar, non polar and semi polar solvent like alcohol,
ether, water, acetone etc.
24. IDENTIFICATION TEST
Goldbeater’s skin test- The Goldbeater’s skin (membrane prepared from the intestine of
ox) is soaked with HCl. Then it is rinsed with distilled water and is added to the tannin
solution (sample) for 5 minutes. It is washed with distilled water and transferred to 1%
ferrous sulphate solution. A brown black colour on the skin confirms the presence of
tannins.
Phenazone test- 10ml of aqueous extract of tannins is prepared and 1gm of sodium acid
phosphate is added. Warm it, cool and filter it. To the filtrate 2% phenazone solution is
added. All the tannins present are precipitated.
Gelatin test- To the solution of tannins add 1% gelatin solution containing 10% sodium
chloride. The precipitate obtain confirms the presence of tannin.
25. Test with ferric chloride solution- To the solution of tannins add ferric chloride solution.
A blue, black, violet, or green precipitate or colour confirm the presence of tannin.
Match-Stick test- Dip a match stick in plant extract and dry it. Moisten it with conc. Hcl
and warm near the flame. The wood of match stick turns to pink or red in colour which
confirms the presence of tannins (On heating tannins with conc. Hcl produce
phloroglucinol. Further phloroglucinol react with the lignin of wood and produce pink
colour