Attuluri Vamsi Kumar, MLT, SMAS, Galgotais Unversity.

1. Module- 5
Topic name
Hepatitis virus
Sub code: BMLT5001
Sub Name: Virology and mycology
Department: Department of MLT, SMAS
Faculty: A. Vamsi Kumar
Designation : Assistant professor
Sem- V

2. SYLLABUS OVERVIEW
Unit-1 Mycology 8 hours
Introduction to medical mycology, basic concepts about superficial and deep mycoses taxonomy , classification &
general characteristics of Various medically important fungi.
Unit-2 Fungal infection 8 hours
Names of fungi & diseases caused by them; superficial mycoses, candida, dermatophytes, opportunistic fungi,
subcutaneous mycoses
Unit-3 Virology 7 hours
Introduction, classification of virus, collection, Transport, & Storage of sample for Viral diagnosis. Staining Techniques
used in virology,
Processing of sample for viral diagnosis (Egg Inoculation & Tissue culture)
Unit-4 RNA Viruses 9 hours
RNA Viruses: Polio myelitis, Rhino virus, Influenza, Rabies, Arbo viruses, Measles Mumps, Rubella, HIV, COVID-19
Unit-5 DNA Viruses 8 hours
DNA viruses: Small Pox, HSV, CMV, EBV, Varicella Zoster, Hepatitis, Adeno virus
Unit - 6 Next generation PCR and it’s modren applications 8 Hours
PCR and Next genration DNA Secquencing techniques to identify viral genome: History of PCR, Priciple of PCR, Stages
of PCR, procedure of PCR, Concept of optimization of PCR, Applications of PCR, Advancements in PCR technology, DNA
sequecing techniques, Methods of DNA Sequencing, Next generation DNA sequencing techniques

3. Unit-4 RNA Viruses 9
hours
RNA Viruses: Polio myelitis, Rhino virus, Influenza, Rabies,
Arbo viruses, Measles, Mumps, Rubella, HIV, COVID-19
Unit 4

4. Unit-5 DNA Viruses
DNA viruses: Small Pox, HSV, CMV, EBV, Varicella Zoster,
Hepatitis, Adeno virus
Unit - 5
Unit - 5

5. World Hepatitis Day
July 28th
A webinar to raise awareness of viral Hepatitis
Organized by
Department of Medical Lab Technology
SMAS
Date & Time: 28th July 1:30PM Platform: MS Teams
Join us
@
https://teams.microsoft.com/l/meetup-
join/19%3a4e47cc08b3464a17ba4fe75105df909c%40thread.tacv2/1595890416
535?context=%7b%22Tid%22%3a%22d988180a-487c-497e-b3d8-
ee0146495fcb%22%2c%22Oid%22%3a%225297b80c-7ba3-4188-a699-
d7c57d66ccd2%22%7d

6. Functions of Liver

7. For every 40 seconds 1 person dies due to
viral Hepatitis
• Hepatitis causes
1. Liver Inflammation
2. Liver Damage
3. Liver Cancer
4. Liver Death
It claims over 1.5 million deaths per year.
Nearly 325 million people suffering with hepatitis infection worldwide.

8. In Canada…
• About 1 in 5 people infected with hepatitis C do not
know they are infected and remain undiagnosed
• An estimated 290 million people have viral
hepatitis B or C, with many unaware of
their status

9. 2020 Theme
Worldwide, 290 million people are living with viral hepatitis unaware.
Without finding the undiagnosed and linking them to care, millions will
continue to suffer, and lives will be lost.
On World Hepatitis Day, 28 July, we call on people from across the world
to take action and raise awareness to find the “missing
millions”.

10. Significance of World hepatitis day
• World Hepatitis Day is recognized annually on July
28th, the birthday of Dr. Baruch Blumberg (1925–
2011).
• Dr. Blumberg discovered the hepatitis B virus in 1967,
and 2 years later he developed the first hepatitis B
vaccine.
• These achievements culminated in Dr. Blumberg
winning the Nobel Prize.
Dr. Baruch Blumberg

13. What is Hepatitis?
• Disease caused by viruses that attack the liver
• There are 5 different types of hepatitis:
Hepatitis
A
B
C
D
E
Cause
Liver damage (cirrhosis)

15. What are the Symptoms?
• Viral hepatitis’ symptoms may not be obvious until serious
liver damage has occurred
Most
common
symptoms
yellowing
of the
skin &
eyes
dark
urine
nausea
&
vomiting
abdominal
pain
fatigue

16. How Do You Get Hepatitis?
Transmission
Sharing drug
using
equipment
Exposure to bodily
fluids of an infected
person (e.g. sexual
intercourse, mother
to child)
Sharing
personal
hygiene
items
Using non-
sterile
equipment for
tattoos & body
piercing
During medical
procedures where
infection control
precautions are
not observed

17. Are there Vaccines?
Hepatitis A & B can be prevented by a vaccine.
There is NO licensed vaccine for hepatitis C.

19. Are there Treatments?
Treatments are available for
both chronic hepatitis B and C.

20. A hepatitis-free future is achievable with a united effort
• PREVENT infection among newborns. All newborns should be vaccinated
against hepatitis B at birth, followed by at least 2 additional doses.
• STOP TRANSMISSION from MOTHER to CHILD. All pregnant women should
be routinely tested for hepatitis B, HIV and syphilis and receive treatment if
needed.
• LEAVE NO ONE BEHIND. Everyone should have access to hepatitis prevention,
testing and treatment services, including people who inject drugs, people in
prisons, migrants, and other highly-affected populations.
• EXPAND access to testing and treatment. Timely testing and treatment of viral
hepatitis can prevent liver cancer and other severe liver diseases.
• MAINTAIN essential hepatitis services during COVID-19. Prevention and care
services for hepatitis - such as infant immunization, harm reduction services and
continuous treatment of chronic hepatitis B - are essential even during the
pandemic.

21. Thank You

22. Families of viruses
DNA Viruses
HAP3
Herpes viridae
1. Alpha – HS1, HS2, VZ
2. Beta – CMV
3. Gama - EBV
DNA “HAS” the potential
ii. Adenovirus
iii. Small pox
iv. Hepatitis
H

23. General points
1. Introduction
2. Classification
3. Morphology (General properties)
• Size
• Shape
• Symmetry
• Capsomers
• Genome
• Envelop
• Special viral proteins
4. Susceptibility & Resistance
5. Isolation / Cultivation - Animal/tissue/cell
culture
6. Pathogenesis + mode of transmission +
Mechanism + incubation period
7. Signs & Symptoms + Complications
8. Laboratory diagnosis
• Sample collection (antemortem + Post
mortem )
• Transport
• Direct microscopy
• Culture / isolation of virus
• Cytopathic effects
• Inclusion bodies or other tests
• Immunofluorescence tests
• Molecular tests
• Ag detection tests
• Detection of viral specific antibodies
9. Prophylaxis (Vaccination)
• Active immunization
• Passive immunization
10. Treatment
1. I
2. C
3. M
4. S&R
5. Cul
6. Patho
7. Signs
8. LD
9. Px
10.T

24. INTRODUCTION

29. Contribution of Dr. Blumberg
• World Hepatitis Day is recognized annually on July
28th, the birthday of Dr. Baruch Blumberg (1925–
2011).
• Dr. Blumberg discovered the hepatitis B virus in 1967,
and 2 years later he developed the first hepatitis B
vaccine.
• These achievements culminated in Dr. Blumberg
winning the Nobel Prize.
Dr. Baruch Blumberg

32. INTRODUCTION

34. Hepatitis A

35. Typical course of Hepatitis A Infection

36. Lab Diagnosis

37. Prophylaxis & Treatment

39. Hepatitis B Virus

40. INTRODUCTION

43. Morphology
1. Size – 22nm/ 27nm / 42nm
2. Shape – Spherical
3. Symmetry –Icosahedral
4. Genome – dsDNA
5. Envelop – Present
6. Viral proteins –
1. HBs Ag
2. HBc Ag
3. Hbe Ag

46. Morphology
1. Size – 22nm/ 27nm / 42nm
2. Shape – Spherical
3. Symmetry –Icosahedral
4. Genome – dsDNA
5. Envelop – Present
6. Viral proteins –
1. HBs Ag
2. HBc Ag
3. Hbe Ag

49. Clinical Symptoms

51. Viral Markers

52. Serological tests in Hepatitis B

53. Laboratory diagnosis

57. Serological tests in Hepatitis B

60. Prophylaxis

63. Treatment