The hamstring muscles are a group of muscles located on the back of the thigh. They include the semitendinosus, semimembranosus, and biceps femoris muscles. Together these muscles act as flexors of the knee and extensors/medial rotators of the hip. They all originate from the ischial tuberosity and are innervated by the tibial nerve. The document provides details on the origin, insertion, action and innervation of each individual hamstring muscle. It also discusses the clinical significance of hamstring injuries and testing.