This document outlines the steps for performing a Gram stain technique lab, which is aimed to differentiate between Gram positive and Gram negative bacteria based on differences in their cell wall structures. The lab report sections include objectives, theory, hypothesis, materials, reagents, procedure, data collection, calculations, analysis, and conclusions. Materials listed are glass slides, inoculating loop, Bunsen burner, bibulous paper, microscope, lens cleaner, immersion oil, distilled water, and 18-24 hour bacterial cultures. Reagents include crystal violet stain, Gram's iodine mordant, ethyl alcohol decolorizer, and safranin secondary stain.

1. GRAM STAIN TECHNIQUE
LAB PRACTICE
EIGHTH GRADE
Guided Stage.
LAB REPORT
1. Title: Gram Stein Technique.
2. Objectives:
 Identify the lab practices as axis of work in the biology
 Differentiate between the two major categories of bacteria: Gram positive and Gram negative.
 understand how the Gram stain reaction affects Gram positive and Gram negative bacteria based
on the biochemical and structural differences of their cell walls.
 Consolidate the knowledge with a lab practice.
3. Theory: Information related with Bacteria and the Gram Stain Technique.
4. Hypothesis: a prediction about the experiment, which will be the result.
5. Equipment, instruments and materials used: Description, function and drawing of each one.
6. Procedure.
7. Data and information.
8. Calculations and results.
9. Analysis.
10. Conclusions.
11. References.
MATERIALS
1. Clean glass slides
2. Inoculating loop
3. Bunsen burner
4. Bibulous paper
5. Microscope
6. Lens paper and lens cleaner
7. Immersion oil
8. Distilled water
9. 18 to 24 hour cultures of organisms

2. REAGENTS
1. Primary Stain - Crystal Violet
2. Mordant - Grams Iodine
3. Decolourizer - Ethyl Alcohol
4. Secondary Stain - Safranin
Available on: http://vlab.amrita.edu/?sub=3&brch=73&sim=208&cnt=2