Next generation DNA sequencing technologies have several key differences from traditional Sanger sequencing. They amplify DNA libraries in vitro instead of cloning into bacteria, determine sequences through nucleotide addition to the complementary strand rather than chain termination, and sequence many spatially segregated and amplified templates simultaneously in a massively parallel way without needing physical separation.

2. Summary
Deoxyribonucleic Acid
Watson and Crick model
DNA Sequencing
Purpose
DNA Sequencing Method
Maxam & Gilbert Method
Sanger Method
Comparison
Application of DNA Sequencing
Advantages & Disadvantages

16. Shotgun sequencing

17. Direct approach
Direct approach

18. Next generation DNA sequencing
Next generation sequencing platforms differ from traditional Sanger sequencing
technology in a number of ways.
First, the DNA sequencing libraries are clonally amplified in vitro, obviating the
need for time consuming and laborious cloning of the DNA library into bacteria
Second, the DNA is sequenced by synthesis, such that the DNA sequence is
determined by the addition of nucleotides to the complementary strand rather
through chain termination chemistry.
Finally, the spatially segregated, amplified DNA templates are sequenced
simultaneously in a massively parallel fashion without the requirement for a
physical separation step.