Fourier Transform Infrared Spectroscopy-:A type of infrared spectroscopy.It is method of obtaining an infrared spectrum by measuring interferogram and then performimg a Fourier Transform upon the interferogram to obtain the spectrum.
An Infrared spectrum represents a fingerprint of a sample with absorption peaks which correspond to the frequencies of vibrations between the bonds of the atoms making up the material-Because each different material is a unique combination of atoms, no two compounds produce the exact same spectrum, therefore IR can result in a unique identification of every different kind of material!
Fourier transform infrared spectroscopy: advantage and disadvantage of conventional infrared spectroscopy, introduction to FTIR ,principle of FTIR, working, advantage, disadvantage and application of FTIR.
IR SPECTROSCOPY, INTRODUCTION, PRINCIPLE, THEORY, FATE OF ABSORBED RADIATION, FERMI RESONANCE, FINGERPRINT REGION, VIBRATIONS, FACTORS AFFECTING ABSORPTION OF IR RADIATION, SAMPLING TECHNIQUES, APPLICATIONS OF IR SPECTROSCOPY.
An Infrared spectrum represents a fingerprint of a sample with absorption peaks which correspond to the frequencies of vibrations between the bonds of the atoms making up the material-Because each different material is a unique combination of atoms, no two compounds produce the exact same spectrum, therefore IR can result in a unique identification of every different kind of material!
Fourier transform infrared spectroscopy: advantage and disadvantage of conventional infrared spectroscopy, introduction to FTIR ,principle of FTIR, working, advantage, disadvantage and application of FTIR.
IR SPECTROSCOPY, INTRODUCTION, PRINCIPLE, THEORY, FATE OF ABSORBED RADIATION, FERMI RESONANCE, FINGERPRINT REGION, VIBRATIONS, FACTORS AFFECTING ABSORPTION OF IR RADIATION, SAMPLING TECHNIQUES, APPLICATIONS OF IR SPECTROSCOPY.
various parts of mAss spectroscopy, applications, principle, peaks, rules, typical mass spectra, various combinations, Fragmentation, rules of fragmentation and useful points which can help Chemical and analytical students and structural elucidation.
This presentation gives you thorough knowledge about the IR Spectroscopy. This include basic principle, type of vibrations, factors influencing vibrational frequency, instrumentation and applications of IR Spectroscopy. This is the most widely used technique for identifying unknown functional group depending on the vibrational frequency.
A method of obtaining an Infrared spectrum by measuring the interferogram of a sample using an interferometer, then performing a Fourier Transform upon the interferogram to obtain the spectrum.
Detectors are the brain of any chromatograhic system. It help us to record the chromatogram based on certain characteristics of the analyte and help us in identifying that compound both qualitatively and quantitatively.
principle, application and instrumentation of UV- visible Spectrophotometer Ayetenew Abita Desa
This Presentation powerpoint includes the principle, application, and instrumentation of UV- Visible Spectrophotometer. It covers beer-lambert low and its quantitative applications. It also includes the qualitative applications in different fields of study. Presented at Addis Ababa University, School of medicine, department of medical biochemistry.
various parts of mAss spectroscopy, applications, principle, peaks, rules, typical mass spectra, various combinations, Fragmentation, rules of fragmentation and useful points which can help Chemical and analytical students and structural elucidation.
This presentation gives you thorough knowledge about the IR Spectroscopy. This include basic principle, type of vibrations, factors influencing vibrational frequency, instrumentation and applications of IR Spectroscopy. This is the most widely used technique for identifying unknown functional group depending on the vibrational frequency.
A method of obtaining an Infrared spectrum by measuring the interferogram of a sample using an interferometer, then performing a Fourier Transform upon the interferogram to obtain the spectrum.
Detectors are the brain of any chromatograhic system. It help us to record the chromatogram based on certain characteristics of the analyte and help us in identifying that compound both qualitatively and quantitatively.
principle, application and instrumentation of UV- visible Spectrophotometer Ayetenew Abita Desa
This Presentation powerpoint includes the principle, application, and instrumentation of UV- Visible Spectrophotometer. It covers beer-lambert low and its quantitative applications. It also includes the qualitative applications in different fields of study. Presented at Addis Ababa University, School of medicine, department of medical biochemistry.
Theory and Principle of FTIR head points:
What is Infrared Region?
Infrared Spectroscopy
What is FTIR?
Superiority of FTIR
FTIR optical system diagram
sampling techniques
The sample analysis process
advantage of FTIR
References
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Ultraviolet-visible spectroscopy or ultraviolet-visible spectrophotometry (UV-Vis or UV/Vis) refers to absorption spectroscopy or reflectance spectroscopy in the ultraviolet-visible spectral region. Ultraviolet-Visible (UV-VIS) Spectroscopy is an analytical method that can measure the analyte quantity depending on the amount of light received by the analyte.
The detailed information of UV Visible Spectroscopy, it includes the information regarding electronic transitions, Electromagnetic radiations, Various shifts.
This pdf is about the Schizophrenia.
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Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
2. Spectroscopy is the science which study
the interaction between the radiation
and matter
Historically, spectroscopy originated
through the study of visible light
dispersed according to its wavelength,
by a prism
3.
4. Some Spectroscopy Types:-
Ultraviolet and Visible Spectoscopy
Fluorescence spectroscopy
Infrared and Raman Spectroscopy
X-ray Diffraction
Atomic Spectroscopy
Circular dichroism Spectroscopy
5. Infrared Spectroscopy:-
• In infrared spectroscopy, IR radiation is passed through a sample,
Some of the infrared radiation is absorbed by the sample and some
of it is passed through (transmitted). The resulting spectrum
represents the molecular absorption and transmission, creating a
molecular fingerprint of the sample.
• Like a fingerprint no two unique molecular structures produce the
same infrared spectrum.
• This makes infrared spectroscopy useful for several types of
analysis.
6. FTIR Spectoscopy:-
A method of obtaining an Infrared spectrum by measuring the
interferogram of a sample using an interferometer, then
performing a Fourier Transform upon the interferogram to obtain
the spectrum.
.
7. Principle:-
• FTIR relies on the fact that the most molecules absorb light in the
infra-red region of the electromagnetic spectrum.
• This absorption corresponds specifically to the bonds present in
the molecule. The frequency range are measured as wave
numbers typically over the range 4000 – 600 cm-1.
• The background emission spectrum of the IR source is first
recorded, followed by the emission spectrum of the IR source
with the sample in place.
8. • The ratio of the sample spectrum to the background spectrum
is directly related to the sample's absorption spectrum.
• The resultant absorption spectrum from the bond natural
vibration frequencies indicates the presence of various
chemical bonds and functional groups present in the sample.
• FTIR is particularly useful for identification of organic
molecular groups and compounds due to the range of
functional groups, side chains and cross-links involved, all of
which will have characteristic vibrational frequencies in the
infra-red range.
10. • To make an interferogram, we combine light from two
different sources. In practice, we use the same light source (a
laser ) and split the light into two beams.
• One is the reference beam, which will provide a comparison
wavefront. The other is the test beam , which is passed
through the optical system to be tested. The two beams are
combined together to make an interferogram
• Most interferometers employ a beamsplitter which takes
the incoming infrared beam and divides it into two optical
beams.
11. • One beam reflects off of a flat mirror which is fixed in place.
The other beam reflects off of a flat mirror which is on a
mechanism which allows this mirror to move a very short
distance (typically a few millimeters) away from the
beamsplitter.
• The two beams reflect off of their respective mirrors and are
recombined when they meet back at the beamsplitter.
Because the path that one beam travels is a fixed length and
the other is constantly changing as its mirror moves, the
signal which exits the interferometer is the result of these
two beams “interfering” with each other.
• The resulting signal is called an interferogram which has the
unique property that every data point (a function of the
moving mirror position) which makes up the signal has
information about every infrared frequency
12. Sampling Techniques:-
1.Liquid Samples:
Neat sample
Diluted solution
Liquid cell
2.Solid Samples:
Neat sample
Cast films
Pressed films
KBr pellets
Mull
3.Gas Samples:
Short path cell
Long path cell
14. The normal instrumental process is as follows:
1.The Source:-
Infrared energy is emitted from a glowing black-
body source. This beam passes through an aperture which
controls the amount of energy presented to the sample (and,
ultimately, to the detector).
2.The Interferometer: -
The beam enters the interferometer
where the “spectral encoding” takesplace. The resulting
interferogram signal then exits the interferometer.
15. 3.The Sample:
The beam enters the sample compartment where it
is transmitted through or reflected off of the surface of the
sample, depending on the type of analysis being accomplished.
This is where specific frequencies of energy, which are uniquely
characteristic of the sample, are absorbed.
4.The Detector:
The beam finally passes to the detector for final
measurement. The detectors used are specially designed to
measure the special interferogram signal
5.The Computer:
The measured signal is digitized and sent to
the computer where the Fourier transformation takes place. The
final infrared spectrum is then presented to the user for
interpretation.
16. Dispersive IR Fourier transform IR
1. There are many moving parts,
resulting in mechanical slippage.
2. Calibration against reference spectra
is required to measure frequency.
3. Stray light causes spurious readings.
4. To improve resolution only a small
amount of IR beam is allowed to pass.
1. Only the mirror moves during the
experiment.
2. Use of laser provides high frequency
accuracy (to 0.01 cm-1).
3. Stray radiations do not affect the
detector.
4. A much larger beam may be used at
all time. Data collection is easier.
17. Applications of FTIR:-
• Identification of simple mixtures of organic and inorganic compounds both as
solids or liquids.
• Identification of polymers and polymer blends.
• Indirect verification of trace organic contaminants on surfaces
• Analysis of Analysis of adhesives, coatings and adhesion promoters or coupling agents.
• Small visible particle chemical analysis..
• Identification of rubbers and filled rubbers.
• Determination of degrees of crystallinity in polymers (eg LDPE and HDPE).
• Compararive chain lengths in organics.
• Extent of thermal, UV or other degredation or depolymerisation of polymers and paint
coatings.
• Analysis of a gaseous samples using a gas cell for headspace analysis or environmental
monitoring.
• Analysis of unknown solvents, cleaning agents and detergents.
18. References:-
1. Principles and Techniques of Biochemistry and Molecular Biology
-Keith Wilson And John Walker
2. Introduction to Spectroscopy
-Donald A. Pavia
3. http://www.lpdlabservices.co.uk/
4. //en.wikipedia.org