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Flagellates
Presented by Ekta Gupta
Chilomastix
Restortamonas
Dientamoeba
Chilomastix Mesnili
 Chilomastix mesnili is a harmless commensal
of cecum and colon in man.
 It is world wide in distribution, found more
frequently in warm climate.
 It has two stages
1. Trophozoite
2. Cyst
1) Trophozoites
1. Pear-shaped
2. Measuring 5–20 µm in length
and 5–10 µm in breadth
3. At the anterior end, it has a
single nucleus called as
cytosome
4. It has four flagella
5. It shows stiff, rotatory
movement
2) CYST
1. It is the infective stage
2. Transmission by fecal-oral route
3. A distinct spiral groove is seen on one side of the nucleus.
4. The cysts are lemon-shaped having a spiral projection at the
anterior end.
5. It measures 5–10 µm in length and 4–6 µm in breadth and is
surrounded by a thick cyst wall.
6. Both trophozoites and cysts are demonstrated in the semi-
formed stool.
7. It is a harmless commensal of caecum where the organism
feeds on bacteria and food debris. Since infection is acquired
through ingestion of cysts, prevention depends on improved
personal hygiene.
Life cycle
Laboratory Diagnosis
 Both the form can be demonstrated by
permanent staining of the stool sample
 Since commensal, so no treatment is required
Retortamonas Intestinalis
 Wenyon and O’ Connor first observed the
parasite in stool in Egypt.
 R. intestinalis is a small non-pathogenic
flagellate found in the large intestine.
 It also exists in 2 forms—
1. Trophozoite
2. Cyst.
1) Trophozoite
 The trophozoite is elongagted, pyriform in
shape,
 Measuring 5–10 µm in length and 3–4 µm
in breadth. €
 The cytoplasm is granular and vacuolated. €
 It has a cleft like cytosome, spherical
nucleus, and central karyosome. €
 Two minute blepharoplasts are present
near nucleus, from which two flagella
originate. €
 It shows jerky movement.
 The trophozoite multiplies by binary fission.
2) Cyst
 It is the infective stage
 The cyst is ovoid or pyriform in
shape
 Measuring 6 µm in length and 3
µm in breadth.
 Infection is transmitted by
ingestion of contaminated cyst.
Laboratory Diagnosis
1. Both the forms can be
demonstrated by
permanent staining of
the stool samples.
2. Prevention depends on
improved personal
hygiene.
Dientamoeba Fragilis
 D. fragilis was previously considered as an
amoeba but has now been reclassifi ed as an
amoeboflagellate, based on electron
microscopic study and antigenic similarity to
Trichomonas.
 Dientamoeba Fragilis lives in the lumen of
the cecum and upper colon of humans.
 It exists in three forms-1] Trophozoite
2] Precyste
3] Cyst
1) Trophozoite
 It is irregular in shape (amoeboid)
 Measures 9 µm to 12 µm
 The name Dientamoeba fragilis is derived from the
binucleate nature of trophozoite (Dientamoeba) and
the fragmented appearance (fragilis) of its nuclear
chromatin.
 It is motile with broad hyaline leaf like pseudopodia.
2) Precyste
 It is spherical 4-5 µm size with a darkly stained
homogenous cytoplasm.
3) Cyst
 Measures 5-8 µm
 Oval to round with 1-2 nuclei.
Laboratory diagnosis
 Stool examination
 Culture
 Antigen detection in stool
 Antibody detection in serum
 Molecular methods
Treatment
 Tetracycline
 metronidazole
THANK YOU

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Flagellates

  • 1. Flagellates Presented by Ekta Gupta Chilomastix Restortamonas Dientamoeba
  • 2. Chilomastix Mesnili  Chilomastix mesnili is a harmless commensal of cecum and colon in man.  It is world wide in distribution, found more frequently in warm climate.  It has two stages 1. Trophozoite 2. Cyst
  • 3. 1) Trophozoites 1. Pear-shaped 2. Measuring 5–20 µm in length and 5–10 µm in breadth 3. At the anterior end, it has a single nucleus called as cytosome 4. It has four flagella 5. It shows stiff, rotatory movement
  • 4. 2) CYST 1. It is the infective stage 2. Transmission by fecal-oral route 3. A distinct spiral groove is seen on one side of the nucleus. 4. The cysts are lemon-shaped having a spiral projection at the anterior end. 5. It measures 5–10 µm in length and 4–6 µm in breadth and is surrounded by a thick cyst wall. 6. Both trophozoites and cysts are demonstrated in the semi- formed stool. 7. It is a harmless commensal of caecum where the organism feeds on bacteria and food debris. Since infection is acquired through ingestion of cysts, prevention depends on improved personal hygiene.
  • 6. Laboratory Diagnosis  Both the form can be demonstrated by permanent staining of the stool sample  Since commensal, so no treatment is required
  • 7. Retortamonas Intestinalis  Wenyon and O’ Connor first observed the parasite in stool in Egypt.  R. intestinalis is a small non-pathogenic flagellate found in the large intestine.  It also exists in 2 forms— 1. Trophozoite 2. Cyst.
  • 8. 1) Trophozoite  The trophozoite is elongagted, pyriform in shape,  Measuring 5–10 µm in length and 3–4 µm in breadth. €  The cytoplasm is granular and vacuolated. €  It has a cleft like cytosome, spherical nucleus, and central karyosome. €  Two minute blepharoplasts are present near nucleus, from which two flagella originate. €  It shows jerky movement.  The trophozoite multiplies by binary fission.
  • 9. 2) Cyst  It is the infective stage  The cyst is ovoid or pyriform in shape  Measuring 6 µm in length and 3 µm in breadth.  Infection is transmitted by ingestion of contaminated cyst.
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  • 11. Laboratory Diagnosis 1. Both the forms can be demonstrated by permanent staining of the stool samples. 2. Prevention depends on improved personal hygiene.
  • 12. Dientamoeba Fragilis  D. fragilis was previously considered as an amoeba but has now been reclassifi ed as an amoeboflagellate, based on electron microscopic study and antigenic similarity to Trichomonas.  Dientamoeba Fragilis lives in the lumen of the cecum and upper colon of humans.  It exists in three forms-1] Trophozoite 2] Precyste 3] Cyst
  • 13. 1) Trophozoite  It is irregular in shape (amoeboid)  Measures 9 µm to 12 µm  The name Dientamoeba fragilis is derived from the binucleate nature of trophozoite (Dientamoeba) and the fragmented appearance (fragilis) of its nuclear chromatin.  It is motile with broad hyaline leaf like pseudopodia.
  • 14. 2) Precyste  It is spherical 4-5 µm size with a darkly stained homogenous cytoplasm. 3) Cyst  Measures 5-8 µm  Oval to round with 1-2 nuclei.
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  • 16. Laboratory diagnosis  Stool examination  Culture  Antigen detection in stool  Antibody detection in serum  Molecular methods