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PROTOZOA
Lab/1 Microbiology II Asst. Lecturer Sara S. H.
Parasitology : is the study of relationships
between parasites and their host , all parasitic
organisms are eukaryotes .
Parasites : are living organisms, which depend
on a living host for their nourishment and survival
.
 parasites can be classified as: (Ectoparasite
inhabit only the body surface of the host without
penetrating the tissue e.g. lice, ticks) or
(Endoparasite which lives within the body of the
host e.g. malaria, giardia ).
 parasites may be simple unicellular protozoa or
complex multicellular metazoa.
Host : an organism, which harbors the
parasite and provides nourishment and is
relatively larger than the parasite .
Definitive host: the organism in which the
adult or sexually mature stage of the parasite
lives.
Intermediate host : the organism in which the
parasite lives during a period of its development
only.
Vector: a living carrier that transports a
pathogenic organism from an infected to non
infected host (e.g. the female Anopheles
mosquito that transmits malaria).
Host – parasite relationships
Symbiosis: both host and parasite are
dependent upon each other, none of them suffers
any harm from the association.
Commensalism: only the parasite derives
benefit from association without causing any
injury to the host.
parasitism: the parasite derives benefites and
the host is always harmed due to the association.
Transmission of parasites
1-Food or water contamination (Round worm,
Amoeba,Giardia).
2-Vectors (Sand fly Leishmaniasis, Tse tse fly
Trypanosomiasis).
3-Sexual contact (Trichomonas).
4-Inhalation of contaminated dust or air Pinworm.
5-Skin penetration (Hook worms, Schistosomes).
Structure of protozoa
Nucleus : protozoa contain one nucleus (some
contain more than one in some their life stages)
the nucleus consist of nucleoplasm in which it
contain internal body called nucleolus that
contain DNA & surround by nuclear membrane .
Nucleus is 2 types :
 Vesicular types
 Compact types
Cytoplasm: part outside the nucleus & it
contains a great variety of organelles:
endoplasmic reticulum & ribosomes as in
eukaryotic cells , mitochondria , Golgi apparatus
, contractile vacuoles that stored substances
such as glycogen & food vacuoles .
Locomotion of protozoa
There are many types of locomotion:
1- By pseudopodia : like Entamoeba
2 - By flagella: like Trypanosoma & Leishmania.
3- By cilia : like Balantidium.
4-Gliding : like Toxoplasma & Sarcocystis.
Flagellum : thread like structure arising from
basal granule or kinetoplast , it consist of central
thread & plasmic sheath .
Cilia : small short structure like flagellum arise
from kinetosome , cilia use for Locomotion ,
Feeding , Sensation.
Pseudopodia : temporary locomotion structure
arising and disappear when use.
Feeding of protozoa
There are many type of feeding or nutrition:
1-autotrophic nutrition: in which the
organism is able to live entirely on inorganic
compounds synthesizing its proteins,
carbohydrates & lipids from them.
2-Holophytic nutrition: (plant like) nutrition
in which the organism synthesizes its
carbohydrates by means of chlorophyll.
3-Holozoic nutrition: (animal like) nutrition in
which particulate food is ingested that a
temporary or permanent mouth (cytosome)
temporary opening may be formed by
pseudopods .
4-Saprozoic nutrition: by osmosis or
diffusing across the cell wall.
Or by pinocytosis , in which fluid may be
pinched off thru temporary openings in the body
wall.
 Excretion is by contracted vacuoles through
cell membrance by diffusion or by cytopage.
Reprodaction of protozoa
There are 2 types of reproduction
1- Asexual:
A- Binary fission: most common when the
mother cell divided longitudinally (nucleus &
cytoplasm divided) Trypanosoma in flagellates &
amoebal & ciliates .
B- Multiple fission: its also called schizogony
, in which the nucleus divides repeadelly ,
cytoplasm gathers around each nucleus & then
cytoplasm divides . The dividing cell is known as
aschizont. Each nucleus called merozoite .
C– Budding: in which a small daughter
individual is separated off from the side of the
mother & then grows to full size .
2- Sexual:
A–Conjugation: Tow individuals come
together temporarily & fuse along part of their
length . Exchange of genetic material . Occurs
among ciliates.
B–Syngamy: connection between male
gametes & female gametes or macrogametes to
form zygote. The process of gamete formation
called gametogony.
AMOEBIASIS
Amoebas primitive unicellular microorganisms with
a relatively simple life cycle which can be divided
into two stages:
 Trophozoite – actively motile feeding stage.
 Cyst – resistant, infective stage.
 Their reproduction is through binary fission, e.g.
splitting of the trophozoite or through the
development of numerous trophozoites within
the mature multinucleated cyst.
 Motility is accomplished by extension of
pseudopodia (false foot).
Entamoeba histolytica
Disease name : Amebic dysentery or
Amebiasis.
Site of infection : Large intestine.
 pathogenic ameba have two stages Trophozoite
(vegetative and diagnostic stage ) and Cyst (
infective and diagnostic stage ) .
Morphology of trophozoite:
 Trophozoite of E. histolytica is (15-60)
micrometer in diameter, has a single nucleus
with a small centrally placed karyosome .
 The nuclear chromatin is evenly distributed
along the periphery of the nucleus .
 The fine granular endoplasm may contain
ingested RBCs.
 In the case of dysentery, however, RBCs may
be visible in the cytoplasm, and this feature
is diagnostic for E. histolytica.
Trophozoites
Morphology of cyst:
 Cyst of E. histolytica is (10-20) micrometer in
diameter and contain one to four nuclei.
 Chromatoid bodies are usually present in young
cysts as elongated bars with bluntly rounded
ends.
 Glycogen is usually diffuse, but in young cysts it
is often present as a concentrated mass, staining
reddish brown with iodine.
Cyst
Life cycle of Entamoeba histolytica :
 Infection occurs by ingestion of cysts on focally
contaminated food or hands. The cyst is resistant
to the gastric environment and passes into small
intestine where it decysts.
 The metacyst divides into four and then eight
amoebae which move to the large intestine.
 The organisms encyst for mitosis and are passed
through with feces.(there are no intermediate or
reservoir host).
Life cycle of Entamoeba histolytica
Symptoms : including diarrhea with blood
and mucus, fever and dehydration.
Laboratory diagnosis:
1-Laboratory diagnosis by finding the
characteristic cysts in an iodine stained or
formolether concentration method or a
permanent stained preparation.
Direct microscopy should be done by mixing a
small amount of the specimen in 0.9% sodium
chloride solution.
2-The tests of indirect fluorescent antibody test
(IFAT), or (ELISA) .
Control
Treatment: Metronidazole.
Prevention:
 Amoebiasis is difficult to eradicate because of
the substantial human reservoir of infection.
 The only progress will be through improved
standards of hygiene and better access to clean
water.
 Cysts are destroyed by boiling, but chlorine and
iodine sterilizing tablets are not always effective.
Entamoeba coli
Entamoeba coli are anon-pathogenic ameba with
world wide distribution. Its life cycle is similar to
that of E. histolytica but it does not have an
invasive stage and does not ingest red blood cells.
Morphology of trophozoite:
 Trophozoite is larger than of E. histolytica
ranging from (15-50) micrometer in diameter .It
exhibits blunt pseudopodia with sluggish
movement.
 A permanently stained preparation shows a
nucleus with a moderately large eccentric
karyosome with the chromatin clumped on the
nuclear membrane.
 The cytoplasm appears granular containing
vacuoles with ingested bacteria and other food
particles.
Trophozoites
Morphology of cyst:
 Cyst of E.coli is (15-30) micrometer in diameter
and contain (1-8) nuclei with irregular peripheral
chromatin, karyosomes not central.
 Chromatoid bodies are not frequently seen but
when present they are usually splinter-like with
pointed ends.
 Glycogen is usually diffuse but in young cyst is
occasionally found as a well defined mass, which
stains reddish brown with iodine.
Cyst
Trophozoites
Cyct
 However, the cysts are remarkably variable in
size.
 The mature cyst (with more than four nuclei) is
the distinctive stage to differentiate E. coli from
the pathogenic E .histolytica.
 Entamoeba coli is transmitted in its viable cystic
stage through fecal contamination.
 Ε. coli is non-pathogenic and produces no
symptoms.
 Specific treatment is not indicated since this
amoeba is non-pathogenic.
 The presence of E .coli in stool specimen is
evidence for fecal contamination.
 Prevention depends on better personal hygiene
and sanitary disposal of human excreta.
LAB 1 Protozoa  Protozoa  Protozoa .pptx

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LAB 1 Protozoa Protozoa Protozoa .pptx

  • 1. PROTOZOA Lab/1 Microbiology II Asst. Lecturer Sara S. H.
  • 2. Parasitology : is the study of relationships between parasites and their host , all parasitic organisms are eukaryotes . Parasites : are living organisms, which depend on a living host for their nourishment and survival .  parasites can be classified as: (Ectoparasite inhabit only the body surface of the host without penetrating the tissue e.g. lice, ticks) or (Endoparasite which lives within the body of the host e.g. malaria, giardia ).  parasites may be simple unicellular protozoa or complex multicellular metazoa.
  • 3. Host : an organism, which harbors the parasite and provides nourishment and is relatively larger than the parasite . Definitive host: the organism in which the adult or sexually mature stage of the parasite lives.
  • 4. Intermediate host : the organism in which the parasite lives during a period of its development only. Vector: a living carrier that transports a pathogenic organism from an infected to non infected host (e.g. the female Anopheles mosquito that transmits malaria).
  • 5. Host – parasite relationships Symbiosis: both host and parasite are dependent upon each other, none of them suffers any harm from the association. Commensalism: only the parasite derives benefit from association without causing any injury to the host. parasitism: the parasite derives benefites and the host is always harmed due to the association.
  • 6. Transmission of parasites 1-Food or water contamination (Round worm, Amoeba,Giardia). 2-Vectors (Sand fly Leishmaniasis, Tse tse fly Trypanosomiasis). 3-Sexual contact (Trichomonas). 4-Inhalation of contaminated dust or air Pinworm. 5-Skin penetration (Hook worms, Schistosomes).
  • 7. Structure of protozoa Nucleus : protozoa contain one nucleus (some contain more than one in some their life stages) the nucleus consist of nucleoplasm in which it contain internal body called nucleolus that contain DNA & surround by nuclear membrane . Nucleus is 2 types :  Vesicular types  Compact types
  • 8. Cytoplasm: part outside the nucleus & it contains a great variety of organelles: endoplasmic reticulum & ribosomes as in eukaryotic cells , mitochondria , Golgi apparatus , contractile vacuoles that stored substances such as glycogen & food vacuoles .
  • 9. Locomotion of protozoa There are many types of locomotion: 1- By pseudopodia : like Entamoeba 2 - By flagella: like Trypanosoma & Leishmania. 3- By cilia : like Balantidium. 4-Gliding : like Toxoplasma & Sarcocystis.
  • 10. Flagellum : thread like structure arising from basal granule or kinetoplast , it consist of central thread & plasmic sheath . Cilia : small short structure like flagellum arise from kinetosome , cilia use for Locomotion , Feeding , Sensation. Pseudopodia : temporary locomotion structure arising and disappear when use.
  • 11. Feeding of protozoa There are many type of feeding or nutrition: 1-autotrophic nutrition: in which the organism is able to live entirely on inorganic compounds synthesizing its proteins, carbohydrates & lipids from them. 2-Holophytic nutrition: (plant like) nutrition in which the organism synthesizes its carbohydrates by means of chlorophyll.
  • 12. 3-Holozoic nutrition: (animal like) nutrition in which particulate food is ingested that a temporary or permanent mouth (cytosome) temporary opening may be formed by pseudopods . 4-Saprozoic nutrition: by osmosis or diffusing across the cell wall.
  • 13. Or by pinocytosis , in which fluid may be pinched off thru temporary openings in the body wall.  Excretion is by contracted vacuoles through cell membrance by diffusion or by cytopage.
  • 14. Reprodaction of protozoa There are 2 types of reproduction 1- Asexual: A- Binary fission: most common when the mother cell divided longitudinally (nucleus & cytoplasm divided) Trypanosoma in flagellates & amoebal & ciliates .
  • 15. B- Multiple fission: its also called schizogony , in which the nucleus divides repeadelly , cytoplasm gathers around each nucleus & then cytoplasm divides . The dividing cell is known as aschizont. Each nucleus called merozoite . C– Budding: in which a small daughter individual is separated off from the side of the mother & then grows to full size .
  • 16. 2- Sexual: A–Conjugation: Tow individuals come together temporarily & fuse along part of their length . Exchange of genetic material . Occurs among ciliates. B–Syngamy: connection between male gametes & female gametes or macrogametes to form zygote. The process of gamete formation called gametogony.
  • 17. AMOEBIASIS Amoebas primitive unicellular microorganisms with a relatively simple life cycle which can be divided into two stages:  Trophozoite – actively motile feeding stage.  Cyst – resistant, infective stage.
  • 18.  Their reproduction is through binary fission, e.g. splitting of the trophozoite or through the development of numerous trophozoites within the mature multinucleated cyst.  Motility is accomplished by extension of pseudopodia (false foot).
  • 19. Entamoeba histolytica Disease name : Amebic dysentery or Amebiasis. Site of infection : Large intestine.  pathogenic ameba have two stages Trophozoite (vegetative and diagnostic stage ) and Cyst ( infective and diagnostic stage ) .
  • 20. Morphology of trophozoite:  Trophozoite of E. histolytica is (15-60) micrometer in diameter, has a single nucleus with a small centrally placed karyosome .  The nuclear chromatin is evenly distributed along the periphery of the nucleus .  The fine granular endoplasm may contain ingested RBCs.  In the case of dysentery, however, RBCs may be visible in the cytoplasm, and this feature is diagnostic for E. histolytica.
  • 22. Morphology of cyst:  Cyst of E. histolytica is (10-20) micrometer in diameter and contain one to four nuclei.  Chromatoid bodies are usually present in young cysts as elongated bars with bluntly rounded ends.  Glycogen is usually diffuse, but in young cysts it is often present as a concentrated mass, staining reddish brown with iodine.
  • 23. Cyst
  • 24.
  • 25. Life cycle of Entamoeba histolytica :  Infection occurs by ingestion of cysts on focally contaminated food or hands. The cyst is resistant to the gastric environment and passes into small intestine where it decysts.  The metacyst divides into four and then eight amoebae which move to the large intestine.  The organisms encyst for mitosis and are passed through with feces.(there are no intermediate or reservoir host).
  • 26.
  • 27. Life cycle of Entamoeba histolytica
  • 28. Symptoms : including diarrhea with blood and mucus, fever and dehydration. Laboratory diagnosis: 1-Laboratory diagnosis by finding the characteristic cysts in an iodine stained or formolether concentration method or a permanent stained preparation. Direct microscopy should be done by mixing a small amount of the specimen in 0.9% sodium chloride solution. 2-The tests of indirect fluorescent antibody test (IFAT), or (ELISA) .
  • 29. Control Treatment: Metronidazole. Prevention:  Amoebiasis is difficult to eradicate because of the substantial human reservoir of infection.  The only progress will be through improved standards of hygiene and better access to clean water.  Cysts are destroyed by boiling, but chlorine and iodine sterilizing tablets are not always effective.
  • 30. Entamoeba coli Entamoeba coli are anon-pathogenic ameba with world wide distribution. Its life cycle is similar to that of E. histolytica but it does not have an invasive stage and does not ingest red blood cells.
  • 31. Morphology of trophozoite:  Trophozoite is larger than of E. histolytica ranging from (15-50) micrometer in diameter .It exhibits blunt pseudopodia with sluggish movement.  A permanently stained preparation shows a nucleus with a moderately large eccentric karyosome with the chromatin clumped on the nuclear membrane.  The cytoplasm appears granular containing vacuoles with ingested bacteria and other food particles.
  • 33. Morphology of cyst:  Cyst of E.coli is (15-30) micrometer in diameter and contain (1-8) nuclei with irregular peripheral chromatin, karyosomes not central.  Chromatoid bodies are not frequently seen but when present they are usually splinter-like with pointed ends.  Glycogen is usually diffuse but in young cyst is occasionally found as a well defined mass, which stains reddish brown with iodine.
  • 34. Cyst
  • 36.
  • 37.  However, the cysts are remarkably variable in size.  The mature cyst (with more than four nuclei) is the distinctive stage to differentiate E. coli from the pathogenic E .histolytica.  Entamoeba coli is transmitted in its viable cystic stage through fecal contamination.  Ε. coli is non-pathogenic and produces no symptoms.
  • 38.  Specific treatment is not indicated since this amoeba is non-pathogenic.  The presence of E .coli in stool specimen is evidence for fecal contamination.  Prevention depends on better personal hygiene and sanitary disposal of human excreta.