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Epithelial tissues I.
1.   Simple squamous epithelium
2.   Simple cuboidal epithelium
3.   Simple columnar epithelium
4.   Pseudostratified columnar
     epithelium with cilia
Characteristics of the epithelial cells:
They are tightly packed together with very little intercellular
matrix. (Intercellular junctions: t ight j unct ions, zonulae
adherent es, desm osom es, gap j unct ions).
Epithelia are derived from all t hree em bryonic germ layers.
The principal functions of epithelial tissues are covering and
lining of surfaces (cove r in g e pit h e lia ), absorpt ion of
subtances     (a bsor pt ive    e pit h e liu m ), secret ion  of
substances (gla n du la r e pit h e liu m ), sensation (sensory
epithelium/neuroepithelium).
The epithelial cells rest on a basem ent m em brane (lamina
lucida + lamina densa + lamina fibroreticularis), and are
connected to this membrane by hemi- desmosomes.
The apical surface of the epithelial cells may be equipped
with structures that greatly increase t he surface area
(microvilli,  stereocilia),   and       structures   which    are
responsible for motility (kinocilia, flagellum).
The simple squamous epithelium consists of a single row of
flattened cells. In cross section the cytoplasm of these cells
is extremely attenuated, so that it is hardly visible. The part
of the cell containing the nucleus is enlarged, the nucleus
stands out in relief.

Location: squamous alveolar cells in the lung (type I
pneumocytes), parietal wall of the renal Bowman capsule,
the narrow segment of renal loop of Henle.

Special types of simple squamous epithelium:
1. Endothelium: epithelium lining the inner surface of the
heart and blood vessels
2. Mesothelium: epithelium of serous membranes lining
certain body cavities (pericardium, pleura, peritoneum)
Simple squamous epithelium
    1.Mesenterium (Ag)
                Actually it is not a section,
                but a very thinly stretched
                membrane (mesenterium
                of frog or rat), and each
                side of this membrane
                presents mesothel cells
                arranged mosaically, side
                by side.
Mesothel
cells
                The cell-boundaries are
                visible by silver nitrate
                treatment as silver
                granules deposit in the
                intercellular spaces.
It is formed by one row of cuboidal cells that possess
spherical nuclei .

Location:
   Follicles of the thyroid gland
   Distal and proximal renal tubules
   Epithelium germinativum covering the ovary
   Amniotic epithelium

The cuboidal cells may have resorbing function,
presenting m icr o villi attached to their apical surface
(e.g. proximal renal tubules).
Simple cuboidal epithelium:
                68. Thyroid gland (HE)
                              The simple cuboidal
                              epithelium of the follicles
                              represent an epithelium of
                              both covering and
                              endocrine glandular type
                              involved in the secretion of
                              thyroxin. The cells are
                              flattened in hypofunction
                              of the gland, and they are
Cell nuclei     Follicles     taller in hyperfunction.
                              The cell boundaries are
                              hardly visible, and the
                              cuboidal epithelium might
                              be recognized according to
                              the spherical shape of the
                              nuclei.
Simple columnar epithelium:
It consists of a single row of cells that have cylindrical or
prismatic shape and oval nuclei.

Location:
   In the digestive tract from the cardia to the anal canal.
   Striated ducts of the salivary glands
   Epithelium of the uterus

Cells that have resorbing function present brush border
formed by microvilli that are disposed on the apical surface.

Location of the simple columnar epithelium with brush
border:
   Small and large intestine

Location of the simple ciliated columnar epithelium:
   Oviduct
Simple columnar epithelium:
               49. Gallbladder (HE)
Oval cell nuclei            The inner surface of the
                            gallbladder is lined by
                            simple columnar
                            epithelium equipped with
                            brush border.

                            The cell boundaries are not
                            clearly visible, but this
                            form of epithelium may be
                            recognized by the oval
                            nuclei disposed in one row.
                            The brush border exhibits
                            positive PAS reaction.
The cells form a single row, but their nuclei are disposed at
different levels so that it gives the illusion of stratification.
Every cell is lying on the basal membrane, that s why this is
a type of simple epithelium. Cells that reach the surface
usually present cilia.

Location of the pseudostratified epithelium with stereocilia:
   Ductus epididymidis
   Ductus deferens

Location of the pseudostratified epithelium with kinocilia:
   Respiratory tract to bronchioles
   Auditory tube, lining of the tympanic cavity
   Inner surface of the lacrimal sac
The inner surface of the
               The           trachea is covered by
               epithelial    pseudostratified
               lining of
                             epithelium with kinocilia.
               the trachea
                             Goblet cells appear
                             among the ciliated
                             columnar cells; their
                             secretion product is not
                             stainable with H.E., but
                             exhibits positive PAS
                             reaction.
Unstained
goblet cells
I de n t ify t h e t ype of t h e e pit h e liu m
         in t h e follow in g se ct ion s!
1.    Simple columnar epithelium (gallbladder)
2.    Simple cuboidal epithelium (medulla of kidney)
3.    Pseudostratified ciliated epithelium (respiratory tract)
4.    Simple columnar epithelium (small intestine)
5.    Simple ciliated columnar epithelium (oviduct)
6.    Simple cuboidal epithelium (medulla of kidney)
7.    Pseudostratified ciliated epithelium (respiratory tract)
8.    Simple columnar epithelium (uterus)
9.    Pseudostratified ciliated epithelium (tail of the
      epididymis)
10.   Mesothelium
11.   Pseudostratified ciliated epithelium (tail of the
      epididymis)

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Epithelium i

  • 2. 1. Simple squamous epithelium 2. Simple cuboidal epithelium 3. Simple columnar epithelium 4. Pseudostratified columnar epithelium with cilia
  • 3. Characteristics of the epithelial cells: They are tightly packed together with very little intercellular matrix. (Intercellular junctions: t ight j unct ions, zonulae adherent es, desm osom es, gap j unct ions). Epithelia are derived from all t hree em bryonic germ layers. The principal functions of epithelial tissues are covering and lining of surfaces (cove r in g e pit h e lia ), absorpt ion of subtances (a bsor pt ive e pit h e liu m ), secret ion of substances (gla n du la r e pit h e liu m ), sensation (sensory epithelium/neuroepithelium). The epithelial cells rest on a basem ent m em brane (lamina lucida + lamina densa + lamina fibroreticularis), and are connected to this membrane by hemi- desmosomes. The apical surface of the epithelial cells may be equipped with structures that greatly increase t he surface area (microvilli, stereocilia), and structures which are responsible for motility (kinocilia, flagellum).
  • 4. The simple squamous epithelium consists of a single row of flattened cells. In cross section the cytoplasm of these cells is extremely attenuated, so that it is hardly visible. The part of the cell containing the nucleus is enlarged, the nucleus stands out in relief. Location: squamous alveolar cells in the lung (type I pneumocytes), parietal wall of the renal Bowman capsule, the narrow segment of renal loop of Henle. Special types of simple squamous epithelium: 1. Endothelium: epithelium lining the inner surface of the heart and blood vessels 2. Mesothelium: epithelium of serous membranes lining certain body cavities (pericardium, pleura, peritoneum)
  • 5. Simple squamous epithelium 1.Mesenterium (Ag) Actually it is not a section, but a very thinly stretched membrane (mesenterium of frog or rat), and each side of this membrane presents mesothel cells arranged mosaically, side by side. Mesothel cells The cell-boundaries are visible by silver nitrate treatment as silver granules deposit in the intercellular spaces.
  • 6. It is formed by one row of cuboidal cells that possess spherical nuclei . Location: Follicles of the thyroid gland Distal and proximal renal tubules Epithelium germinativum covering the ovary Amniotic epithelium The cuboidal cells may have resorbing function, presenting m icr o villi attached to their apical surface (e.g. proximal renal tubules).
  • 7. Simple cuboidal epithelium: 68. Thyroid gland (HE) The simple cuboidal epithelium of the follicles represent an epithelium of both covering and endocrine glandular type involved in the secretion of thyroxin. The cells are flattened in hypofunction of the gland, and they are Cell nuclei Follicles taller in hyperfunction. The cell boundaries are hardly visible, and the cuboidal epithelium might be recognized according to the spherical shape of the nuclei.
  • 8. Simple columnar epithelium: It consists of a single row of cells that have cylindrical or prismatic shape and oval nuclei. Location: In the digestive tract from the cardia to the anal canal. Striated ducts of the salivary glands Epithelium of the uterus Cells that have resorbing function present brush border formed by microvilli that are disposed on the apical surface. Location of the simple columnar epithelium with brush border: Small and large intestine Location of the simple ciliated columnar epithelium: Oviduct
  • 9. Simple columnar epithelium: 49. Gallbladder (HE) Oval cell nuclei The inner surface of the gallbladder is lined by simple columnar epithelium equipped with brush border. The cell boundaries are not clearly visible, but this form of epithelium may be recognized by the oval nuclei disposed in one row. The brush border exhibits positive PAS reaction.
  • 10. The cells form a single row, but their nuclei are disposed at different levels so that it gives the illusion of stratification. Every cell is lying on the basal membrane, that s why this is a type of simple epithelium. Cells that reach the surface usually present cilia. Location of the pseudostratified epithelium with stereocilia: Ductus epididymidis Ductus deferens Location of the pseudostratified epithelium with kinocilia: Respiratory tract to bronchioles Auditory tube, lining of the tympanic cavity Inner surface of the lacrimal sac
  • 11. The inner surface of the The trachea is covered by epithelial pseudostratified lining of epithelium with kinocilia. the trachea Goblet cells appear among the ciliated columnar cells; their secretion product is not stainable with H.E., but exhibits positive PAS reaction. Unstained goblet cells
  • 12. I de n t ify t h e t ype of t h e e pit h e liu m in t h e follow in g se ct ion s!
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  • 25. 1. Simple columnar epithelium (gallbladder) 2. Simple cuboidal epithelium (medulla of kidney) 3. Pseudostratified ciliated epithelium (respiratory tract) 4. Simple columnar epithelium (small intestine) 5. Simple ciliated columnar epithelium (oviduct) 6. Simple cuboidal epithelium (medulla of kidney) 7. Pseudostratified ciliated epithelium (respiratory tract) 8. Simple columnar epithelium (uterus) 9. Pseudostratified ciliated epithelium (tail of the epididymis) 10. Mesothelium 11. Pseudostratified ciliated epithelium (tail of the epididymis)