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DISACCHARIDES
DISACCHARIDES
• Sugars composed of two mono saccharide
units joined by α -Glycosidic bond.
• MALTOSE14
• SUCROSE 12
• LACTOSE 14
• LACTULOSE14
• TREHALOSE11
MALTOSE
• COMPOSITION
• O α- D- Glucopyranosyl (1-4) α-D-
Glucopyranosyl
• MALT sugar
• SOURCES
• Malt, germinating cereals, enzymatic
hydrolysis of starch by salivary & pancreatic
Amylases.
• Maltase enzyme hdrolyzes Maltose into
Glucose.
• strong reducing agent.
• Shows muta rotation.
• Forms sun flower shaped Osazone crystals.
ISOMALTOSE
• Enzymatic hydrolysis of branch points of the
AMYLOPECTIN unit of Starch.
• Shows α-(1-6) linkage.
LACTOSE
• SOURCES
• Milk ,pharmaceutical preparations.
• COMPOSITION
• O-β -D- Galactopyranosyl (1-4)-β- D- Glucopyranosyl
• Synthesized in mammary glands ,can appear in the
urine of lactating mothers.---LACTOSURIA.
• COW S’ milk contains 45% Lactose.
• Human milk contains 74% Lactose.
• Reducing sugar ,shows muta rotation.
LACTOSE INTOLERANCE
• Lactase deficiency
• Diarrhea ,flatulence , dehydration .
• Treatment
• Stop breast feeding/ milk intake.
• Yoghurt ,cheese & green vegetables for Ca++
intake.
• Lactase pills.
LACTULOSE
• O-α –D Galactopyranosyl (1-4 )-β-D Fructopyranosyl
• SOURCES
• Heated Milk , synthetically produced .
• Can not be hydrolyzed by intestinal enzymes .
• Fermentation by intestinal bacteria .
• USES
• Mild Diuretic /osmotic laxative .
SUCROSE (TABLE SUGAR )
• O α -D –Glucopyranosyl (1-2) β- D- fructofuranose
sources
• Cane ,beetroot,sorgham,fruits & vegetables.
• Non reducing sugar ,
• Does not form Osazone crystals.
• Does not exhibit muta rotation .
• Dextro rotatory sugar ( + 66.5 )
• On hydrolysis it becomes strongly Levorotatory
(- 28.2)
• INVERT SUGAR
• Hydrolytic products of Sucrose are called invert sugars.
• Sucrose hydrolysis by Sucrase/ dilute acids liberates
one molecule each of α D- Glucopyranose & β –D
Fructofuranose.
• β D- Fructofuranose form is unstable & immediately
gets converted to β –D Fructopyranose form, which is
strongly Levorotatory ( -92 )
• Overall effect is INVERSION of dextro rotatory Sucrose
(+66.5) to Levorotatory.( - 22.8)
• Invert sugars are 33% more sweet than Sucrose i.e.
Honey
TREHALOSE
• O α-D Glucopyranosyl (1-1) α-D Glucopyranosyl
• SOURCES
• Fungi, yeast
OLIGO SACCHARIDES
• 3-10 carbohydrate units.
• Components of integral membrane proteins,
present on their extra cellular domain.
• Mostly contain Mannose /Sialic acid residues.
• Help in degradation of these integral proteins.
• EXAMPLES
• Glycoproteins
• Antibodies
• Coagulation factors

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Disaccharides Invert sugar and other

  • 2. DISACCHARIDES • Sugars composed of two mono saccharide units joined by α -Glycosidic bond. • MALTOSE14 • SUCROSE 12 • LACTOSE 14 • LACTULOSE14 • TREHALOSE11
  • 4. • COMPOSITION • O α- D- Glucopyranosyl (1-4) α-D- Glucopyranosyl • MALT sugar • SOURCES • Malt, germinating cereals, enzymatic hydrolysis of starch by salivary & pancreatic Amylases. • Maltase enzyme hdrolyzes Maltose into Glucose. • strong reducing agent. • Shows muta rotation. • Forms sun flower shaped Osazone crystals.
  • 5.
  • 6. ISOMALTOSE • Enzymatic hydrolysis of branch points of the AMYLOPECTIN unit of Starch. • Shows α-(1-6) linkage.
  • 7. LACTOSE • SOURCES • Milk ,pharmaceutical preparations. • COMPOSITION • O-β -D- Galactopyranosyl (1-4)-β- D- Glucopyranosyl • Synthesized in mammary glands ,can appear in the urine of lactating mothers.---LACTOSURIA. • COW S’ milk contains 45% Lactose. • Human milk contains 74% Lactose. • Reducing sugar ,shows muta rotation.
  • 8.
  • 9. LACTOSE INTOLERANCE • Lactase deficiency • Diarrhea ,flatulence , dehydration . • Treatment • Stop breast feeding/ milk intake. • Yoghurt ,cheese & green vegetables for Ca++ intake. • Lactase pills.
  • 10.
  • 11.
  • 12. LACTULOSE • O-α –D Galactopyranosyl (1-4 )-β-D Fructopyranosyl • SOURCES • Heated Milk , synthetically produced . • Can not be hydrolyzed by intestinal enzymes . • Fermentation by intestinal bacteria . • USES • Mild Diuretic /osmotic laxative .
  • 13. SUCROSE (TABLE SUGAR ) • O α -D –Glucopyranosyl (1-2) β- D- fructofuranose sources • Cane ,beetroot,sorgham,fruits & vegetables. • Non reducing sugar , • Does not form Osazone crystals. • Does not exhibit muta rotation . • Dextro rotatory sugar ( + 66.5 ) • On hydrolysis it becomes strongly Levorotatory (- 28.2)
  • 14.
  • 15. • INVERT SUGAR • Hydrolytic products of Sucrose are called invert sugars. • Sucrose hydrolysis by Sucrase/ dilute acids liberates one molecule each of α D- Glucopyranose & β –D Fructofuranose. • β D- Fructofuranose form is unstable & immediately gets converted to β –D Fructopyranose form, which is strongly Levorotatory ( -92 ) • Overall effect is INVERSION of dextro rotatory Sucrose (+66.5) to Levorotatory.( - 22.8) • Invert sugars are 33% more sweet than Sucrose i.e. Honey
  • 16. TREHALOSE • O α-D Glucopyranosyl (1-1) α-D Glucopyranosyl • SOURCES • Fungi, yeast
  • 17. OLIGO SACCHARIDES • 3-10 carbohydrate units. • Components of integral membrane proteins, present on their extra cellular domain. • Mostly contain Mannose /Sialic acid residues. • Help in degradation of these integral proteins. • EXAMPLES • Glycoproteins • Antibodies • Coagulation factors