13.04.09 Dr..D.Arvind Prasanth1
Classification & General
Properties of Fungi
13.04.09 2
Introduction
 Mykes (Greek word) : Mushroom
 Fungi are eukaryotic protista; differ from
bacteria and other prokaryotes.
1. Cell walls containing chitin (rigidity & support),
mannan & other polysaccharides
2. Cytoplasmic membrane contains ergosterols
3. Possess true nuclei with nuclear membrane &
paired chromosomes.
4. Divide asexually, sexually or by both
5. Unicellular or multicellular
13.04.09 3
Introduction
 Simplest fungus :- Unicellular budding
yeast
 Hypha :- Elongation of apical cell
produces a tubular, thread like structure
called hypha
 Mycelium :- Tangled mass of hyphae is
called mycelium. Fungi producing
mycelia are called molds or filamentous
fungi.
 Hyphae may be septate or non-septate
13.04.09 4
CLASSIFICATION
 Depending on cell morphology
1. Yeasts
2. Yeast like fungi
3. Molds
4. Dimorphic fungi
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1. Yeasts
 Unicellular fungi which
reproduce by budding
 On culture - produce
smooth, creamy colonies
e. g Cryptococcus
neoformans (capsulated
yeast)
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2. Yeast like fungi
 Grow partly as yeasts and partly as
elongated cells resembling hyphae
which are called pseudohyphae.
e.g. Candida albicans
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3. Molds/ Filamentous fungi
 Form true mycelia &
reproduce by formation
of different types of
spores.
 Vegetative/ aerial
hyphae
e.g. Rhizopus, mucor
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4. Dimorphic fungi
 Occur in 2 forms
Molds (Filaments) – 25C (soil)
Yeasts – 37C (in host tissue)
Most fungi causing systemic infections
are dimorphic:
 Histoplasma capsulatum
 Blastomyces dermatidis
 Paracoccidioides brasiliensis
 Coccidioides immitis
 Penicillium marneffei
 Sporothrix schenkii
13.04.09 9
Reproduction in fungi
 Sexual - formation of zygospores,
ascospores or basidiospores
 Asexual reproduction – budding or fission
 Asexual spores are formed on or in
specialized structures.
 Vary in size, shape & colour but these
characteristics are constant for a particular
species.
13.04.09 10
Reproduction in fungi
 Micro conidia -
Small, single
celled
 Macro conidia –
Large, single or
many celled
13.04.09 11
Systematic classification
 Based on sexual spore formation: 4
classes
1. Zygomycetes
2. Ascomycetes reproduce sexually
3. Basidiomycetes
4. Deuteromycetes (fungi imperfectii)
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1. Zygomycetes
 Lower fungi
 Broad, nonseptate hyphae
 Asexual spores -
Sporangiospores: present
within a swollen sac- like
structure called
Sporangium
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1. Zygomycetes
 Sexual spores -
Zygospore: a resting,
thick walled cell in
between hyphae
e.g. Rhizopus, Mucor
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2. Ascomycetes
 Includes both yeasts & filamentous fungi
 Narrow, septate hyphae
 Asexual spores are called conidia borne on conidiophore
13.04.09 15
2. Ascomycetes
 Sexual spores called ascospores
are present within a sac like
structure called Ascus.
 Several asci may be seen within a
fruiting body as seen in Penicillium,
Aspergillus
 Each ascus has 4 to 8 ascospores.
13.04.09 16
3. Basidiomycetes
 Sexual fusion results in the formation of a
club shaped organ called base or basidium
which bear spores called basidiospores
13.04.09 17
4. Deuteromycetes
or Fungi imperfectii
 Group of fungi whose sexual phases are
not identified.
 Grow as molds as well as yeasts.
 Asexual stage – conidia
e.g. Candida, Cryptococcus
13.04.09 18
Vegetative Structures of
Fungi
 Arthrospores –
formed by
segmentation &
condensation of
hyphae
 Chlamydospores –
thick walled resting
spores
e.g. C.albicans
13.04.09 19
Fungal Infections/ Mycoses
 Superficial mycoses:
 2 types: surface and cutaneous mycoses
 Skin, hair & nails.
 Mild but chronic disease
 Deep mycoses:
 2 types: subcutaneous & systemic mycoses
 Caused by soil saprophytes
 Infection is accidental
 Range from a symptomatic infection to fatal disease
13.04.09 20
Superficial: Surface mycoses
 Live exclusively on dead surfaces of
skin and its appendages
 No contact with living tissue, hence no
inflammatory response
1. Tinea versicolor
2. Tinea nigra
3. Piedra
13.04.09 21
Superficial: Cutaneous mycoses
 Cornified layer of skin & its appendages
 Contact with living tissue, hence
inflammatory & allergic responses seen
1. Dermatophytes – skin, hair & nails
2. 3 genera - Trichophyton
Microsporum
Epidermophyton
13.04.09 22
Deep mycoses
Subcutaneous mycoses
1. Mycotic Mycetoma
2. Chromoblastomycoses
3. Sporotrichosis
4. Rhinosporidiosis
Systemic mycoses
1. Cryptococcoses
2. Blastomycosis
3. Coccidioidomycoses
4. Histoplasmoses
13.04.09 23
Candidiasis
 Caused by candida sps, forms a bridge
between superficial & deep mycoses as
it can cause cutaneous as well as
systemic infections
 Can also cause opportunistic infections
13.04.09 24
Opportunistic infections
 Pts with debilitating disease, altered
physiological state
 Mainly caused by fungi which are common lab
contaminant on culture media
 Aspergillus
 Pencillium
 Mucor
 Rhizopus
 Produce serious & fatal infections
13.04.09 25
Useful Properties of Fungi
Source of food
e.g. mushrooms
Fermentation - Production of
alcohol, bread, cheese
e.g. Sacchromyces spps
Antibiotic production
e.g. Penicillin from
Penicillium notatum
13.04.09 26
Useful Properties of Fungi
Ergot from Claviceps
purpurea, used to induce
uterine contractions
Vaccines for Hepatitis B –
Sacchromyces cerevisiae

Classification & properties of fungi

  • 1.
  • 2.
    13.04.09 2 Introduction  Mykes(Greek word) : Mushroom  Fungi are eukaryotic protista; differ from bacteria and other prokaryotes. 1. Cell walls containing chitin (rigidity & support), mannan & other polysaccharides 2. Cytoplasmic membrane contains ergosterols 3. Possess true nuclei with nuclear membrane & paired chromosomes. 4. Divide asexually, sexually or by both 5. Unicellular or multicellular
  • 3.
    13.04.09 3 Introduction  Simplestfungus :- Unicellular budding yeast  Hypha :- Elongation of apical cell produces a tubular, thread like structure called hypha  Mycelium :- Tangled mass of hyphae is called mycelium. Fungi producing mycelia are called molds or filamentous fungi.  Hyphae may be septate or non-septate
  • 4.
    13.04.09 4 CLASSIFICATION  Dependingon cell morphology 1. Yeasts 2. Yeast like fungi 3. Molds 4. Dimorphic fungi
  • 5.
    13.04.09 5 1. Yeasts Unicellular fungi which reproduce by budding  On culture - produce smooth, creamy colonies e. g Cryptococcus neoformans (capsulated yeast)
  • 6.
    13.04.09 6 2. Yeastlike fungi  Grow partly as yeasts and partly as elongated cells resembling hyphae which are called pseudohyphae. e.g. Candida albicans
  • 7.
    13.04.09 7 3. Molds/Filamentous fungi  Form true mycelia & reproduce by formation of different types of spores.  Vegetative/ aerial hyphae e.g. Rhizopus, mucor
  • 8.
    13.04.09 8 4. Dimorphicfungi  Occur in 2 forms Molds (Filaments) – 25C (soil) Yeasts – 37C (in host tissue) Most fungi causing systemic infections are dimorphic:  Histoplasma capsulatum  Blastomyces dermatidis  Paracoccidioides brasiliensis  Coccidioides immitis  Penicillium marneffei  Sporothrix schenkii
  • 9.
    13.04.09 9 Reproduction infungi  Sexual - formation of zygospores, ascospores or basidiospores  Asexual reproduction – budding or fission  Asexual spores are formed on or in specialized structures.  Vary in size, shape & colour but these characteristics are constant for a particular species.
  • 10.
    13.04.09 10 Reproduction infungi  Micro conidia - Small, single celled  Macro conidia – Large, single or many celled
  • 11.
    13.04.09 11 Systematic classification Based on sexual spore formation: 4 classes 1. Zygomycetes 2. Ascomycetes reproduce sexually 3. Basidiomycetes 4. Deuteromycetes (fungi imperfectii)
  • 12.
    13.04.09 12 1. Zygomycetes Lower fungi  Broad, nonseptate hyphae  Asexual spores - Sporangiospores: present within a swollen sac- like structure called Sporangium
  • 13.
    13.04.09 13 1. Zygomycetes Sexual spores - Zygospore: a resting, thick walled cell in between hyphae e.g. Rhizopus, Mucor
  • 14.
    13.04.09 14 2. Ascomycetes Includes both yeasts & filamentous fungi  Narrow, septate hyphae  Asexual spores are called conidia borne on conidiophore
  • 15.
    13.04.09 15 2. Ascomycetes Sexual spores called ascospores are present within a sac like structure called Ascus.  Several asci may be seen within a fruiting body as seen in Penicillium, Aspergillus  Each ascus has 4 to 8 ascospores.
  • 16.
    13.04.09 16 3. Basidiomycetes Sexual fusion results in the formation of a club shaped organ called base or basidium which bear spores called basidiospores
  • 17.
    13.04.09 17 4. Deuteromycetes orFungi imperfectii  Group of fungi whose sexual phases are not identified.  Grow as molds as well as yeasts.  Asexual stage – conidia e.g. Candida, Cryptococcus
  • 18.
    13.04.09 18 Vegetative Structuresof Fungi  Arthrospores – formed by segmentation & condensation of hyphae  Chlamydospores – thick walled resting spores e.g. C.albicans
  • 19.
    13.04.09 19 Fungal Infections/Mycoses  Superficial mycoses:  2 types: surface and cutaneous mycoses  Skin, hair & nails.  Mild but chronic disease  Deep mycoses:  2 types: subcutaneous & systemic mycoses  Caused by soil saprophytes  Infection is accidental  Range from a symptomatic infection to fatal disease
  • 20.
    13.04.09 20 Superficial: Surfacemycoses  Live exclusively on dead surfaces of skin and its appendages  No contact with living tissue, hence no inflammatory response 1. Tinea versicolor 2. Tinea nigra 3. Piedra
  • 21.
    13.04.09 21 Superficial: Cutaneousmycoses  Cornified layer of skin & its appendages  Contact with living tissue, hence inflammatory & allergic responses seen 1. Dermatophytes – skin, hair & nails 2. 3 genera - Trichophyton Microsporum Epidermophyton
  • 22.
    13.04.09 22 Deep mycoses Subcutaneousmycoses 1. Mycotic Mycetoma 2. Chromoblastomycoses 3. Sporotrichosis 4. Rhinosporidiosis Systemic mycoses 1. Cryptococcoses 2. Blastomycosis 3. Coccidioidomycoses 4. Histoplasmoses
  • 23.
    13.04.09 23 Candidiasis  Causedby candida sps, forms a bridge between superficial & deep mycoses as it can cause cutaneous as well as systemic infections  Can also cause opportunistic infections
  • 24.
    13.04.09 24 Opportunistic infections Pts with debilitating disease, altered physiological state  Mainly caused by fungi which are common lab contaminant on culture media  Aspergillus  Pencillium  Mucor  Rhizopus  Produce serious & fatal infections
  • 25.
    13.04.09 25 Useful Propertiesof Fungi Source of food e.g. mushrooms Fermentation - Production of alcohol, bread, cheese e.g. Sacchromyces spps Antibiotic production e.g. Penicillin from Penicillium notatum
  • 26.
    13.04.09 26 Useful Propertiesof Fungi Ergot from Claviceps purpurea, used to induce uterine contractions Vaccines for Hepatitis B – Sacchromyces cerevisiae