Rice breeding at IRGA in Rio Grande do Sul, Brazil focuses on developing cultivars adapted to different regions through breeding programs for general adaptation, hybrids, herbicide tolerance, and abiotic stress tolerance. The breeding program evaluates over 5,000 lines annually for yield, grain quality, disease resistance, and other traits. Recent releases include the high yielding, herbicide tolerant cultivars IRGA 427 and IRGA 428. The hybrid program has released the high yielding hybrid QM 1010 and is developing Clearfield hybrids. Breeding efforts aim to improve yield potential, grain quality, lodging resistance, and tolerance to stresses like iron toxicity and cold temperatures.
The document discusses the DSP BlackRock World Mining Fund, which is structured through BlackRock Global Funds. It invests the assets of Indian investors totaling USD 17.8 billion and focuses on sectors like iron ore, copper, aluminum, coal, gold, nickel, platinum, and diamonds. Charts show the evolution of world GDP over time with countries like China and India growing substantially in recent decades. Finally, it outlines the year-to-date performance of various mining equity indexes as of early 2011.
Presentation of Steffen Noleppa, Agripol, at Food, Fertilizers and Natural Re...Fertilizers Europe
This document discusses how agricultural productivity growth in the EU may help address environmental issues by reducing pressure on global land use. The EU currently imports a significant amount of virtual land through food imports. Increasing EU production and productivity, such as closing yield gaps, could reduce these imports by around 18 million hectares. This would help limit expansion of agriculture into sensitive ecosystems and reduce greenhouse gas emissions from land use change. The document argues policy should refocus on boosting productivity through investments in research and a stable regulatory environment.
The document analyzes Facebook posting strategies and provides recommendations. Key findings include:
1) Engagement rates are declining, with an average of 0.79 engagements per thousand.
2) Post frequency of 6-8 posts per week is optimal.
3) Posts with under 11 words performed best.
Recommended actions include creating relevant engaging content, setting benchmarks, experimenting with different post types, and focusing on quality over quantity of posts.
This document analyzes the relationship between methylation levels and gene expression levels for various MAGE genes. Scatter plots and trend lines show that methylation inhibits or reduces the expression of many MAGE genes, though the relationship varies between genes. Higher methylation is correlated with lower expression for most genes analyzed.
This document provides an overview and summary of ING Group's third quarter 2008 results presentation. Some key points:
- ING reported its first ever quarterly loss due to impacts from market turmoil, though commercial performance remained resilient.
- ING took actions to reinforce its capital base in line with increased international capital standards following government interventions in other banks.
- While earnings were affected by volatility, underlying commercial results were stable with EUR 38 billion in net client balance growth. Expenses remained under control despite challenges in the operating environment.
Perspectives on the Future of Food Security, Review 1 by Geraldo Martha, EMBRAPA on 11 April 2013 at the Food Security Futures I Conference in Dublin, Ireland.
Sorghum breeding for Improved productivity nutrition and INDUSTRIAL useRUFORUM
This document summarizes research on sorghum breeding for improved productivity, nutrition, and industrial use. Over 1,500 new sorghum and maize genotypes have been developed with traits like waxy endosperm and quality protein. A transformation protocol for sorghum was also developed. Marker-assisted selection is being used to map loci for maize streak virus resistance, with one resistance locus identified based on dominance. Modern tools like SSR analysis are assisting the breeding program. The overall goal is to develop specialized sorghum and maize technologies to increase food security and market opportunities in a sustainable way.
The document discusses the DSP BlackRock World Mining Fund, which is structured through BlackRock Global Funds. It invests the assets of Indian investors totaling USD 17.8 billion and focuses on sectors like iron ore, copper, aluminum, coal, gold, nickel, platinum, and diamonds. Charts show the evolution of world GDP over time with countries like China and India growing substantially in recent decades. Finally, it outlines the year-to-date performance of various mining equity indexes as of early 2011.
Presentation of Steffen Noleppa, Agripol, at Food, Fertilizers and Natural Re...Fertilizers Europe
This document discusses how agricultural productivity growth in the EU may help address environmental issues by reducing pressure on global land use. The EU currently imports a significant amount of virtual land through food imports. Increasing EU production and productivity, such as closing yield gaps, could reduce these imports by around 18 million hectares. This would help limit expansion of agriculture into sensitive ecosystems and reduce greenhouse gas emissions from land use change. The document argues policy should refocus on boosting productivity through investments in research and a stable regulatory environment.
The document analyzes Facebook posting strategies and provides recommendations. Key findings include:
1) Engagement rates are declining, with an average of 0.79 engagements per thousand.
2) Post frequency of 6-8 posts per week is optimal.
3) Posts with under 11 words performed best.
Recommended actions include creating relevant engaging content, setting benchmarks, experimenting with different post types, and focusing on quality over quantity of posts.
This document analyzes the relationship between methylation levels and gene expression levels for various MAGE genes. Scatter plots and trend lines show that methylation inhibits or reduces the expression of many MAGE genes, though the relationship varies between genes. Higher methylation is correlated with lower expression for most genes analyzed.
This document provides an overview and summary of ING Group's third quarter 2008 results presentation. Some key points:
- ING reported its first ever quarterly loss due to impacts from market turmoil, though commercial performance remained resilient.
- ING took actions to reinforce its capital base in line with increased international capital standards following government interventions in other banks.
- While earnings were affected by volatility, underlying commercial results were stable with EUR 38 billion in net client balance growth. Expenses remained under control despite challenges in the operating environment.
Perspectives on the Future of Food Security, Review 1 by Geraldo Martha, EMBRAPA on 11 April 2013 at the Food Security Futures I Conference in Dublin, Ireland.
Sorghum breeding for Improved productivity nutrition and INDUSTRIAL useRUFORUM
This document summarizes research on sorghum breeding for improved productivity, nutrition, and industrial use. Over 1,500 new sorghum and maize genotypes have been developed with traits like waxy endosperm and quality protein. A transformation protocol for sorghum was also developed. Marker-assisted selection is being used to map loci for maize streak virus resistance, with one resistance locus identified based on dominance. Modern tools like SSR analysis are assisting the breeding program. The overall goal is to develop specialized sorghum and maize technologies to increase food security and market opportunities in a sustainable way.
NAL Energy Corporation is an oil and gas producer focused on light oil with assets in western Canada. Some key points:
- Market cap of $1.2 billion with monthly dividend of $0.07/share and current yield of 10.4%
- Produces over 28,000 boe/day from assets in Alberta, southeast Saskatchewan, and British Columbia. Reserves of 104 MMBoe with 50% liquids.
- Focused on oil drilling for its 2011 $240 million capital program to maintain production of around 28,500 boe/day for the year. Hedges over 50% of oil volumes.
- Operates across different oil resource plays like the Cardium, V
Mage genes independent biomarker for prognosis 14-02-2012Cheng Luo
1. The document analyzes the correlation between methylation and gene expression of various MAGE genes.
2. Graphs show the relationships between methylation levels and gene expression levels for different MAGE genes.
3. Methylation is found to inhibit the expression of some MAGE genes, which could increase patient survival times.
Deutsche EuroShop is Germany's only public company that invests solely in shopping centers. It owns interests in 18 shopping centers located primarily in Germany, with a total lettable space of around 848,000 square meters. Deutsche EuroShop aims for long-term growth and stable increases in the value of its shopping center portfolio. Its key strategies include a "buy and hold" approach and targeting an annual portfolio extension of 10% primarily through acquisitions in Germany and Europe.
Preliminary participatory on-farm sorghum variety selection for tolerance to ...ILRI
Presented by S. Gudu, E.O. Ouma, A.O. Onkware, E.J. Too, B.A. Were, J.O. Ochuodho, C.O. Othieno, J.R. Okalebo, J. Agalo and S.M. Maina at the First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27 February 2013
The document provides highlights and financial results from Grupo Pão de Açúcar's (GPA) earnings release for fiscal year 2012.
- GPA saw 20.9% revenue growth to R$3,094.3 million in 2012, with same-store sales growth of 14.1%.
- Adjusted EBITDA increased 23% to R$191 million in 2012 with margins of 6.2%.
- GPA opened 96 new owned stores in 2012 and ended the year with 708 owned stores and 388 franchise stores.
- Capex was R$135 million in 2012 focused on organic growth, store renovations, and new distribution centers.
1. REEEP is an international NGO with over 200 partners across governments, businesses, and NGOs committed to accelerating renewable energy and energy efficiency.
2. REEEP is currently funded by 11 governments and the EU and aims to reduce market barriers for renewables and energy efficiency through 50 projects across 40 countries.
3. REEEP focuses on emerging markets and developing countries and believes action and technology transfer are improved through on the ground projects that target policy and innovative financing.
MLN Workshop: Breeding for maize lethal necrosis -- B DasCIMMYT
CIMMYT's maize breeding program focuses on developing varieties tolerant to biotic and abiotic stresses for tropical regions where maize is an important food crop. The program has screened over 45,000 varieties for tolerance to Maize Lethal Necrosis (MLN), finding that around 10% show some tolerance. Promising tolerant inbred lines and hybrids have been identified and some recommended for release. CIMMYT is expanding its MLN screening capacity through additional sites in East Africa that experience natural MLN infection. The goal is to accelerate the development and release of MLN-tolerant varieties to address the devastating impacts of the disease.
Botany is the study of plants. Key points from the document include:
- Plants are multicellular organisms that photosynthesize and produce their food from sunlight. They live on land and are anchored in place.
- Plant cells contain rigid cell walls, central vacuoles, and chloroplasts for photosynthesis.
- Plants are composed of tissues including dermal, ground, and vascular tissues which transport water and nutrients.
- The main plant organs are roots, stems, leaves, flowers, and fruits. Roots take in water and minerals while leaves perform photosynthesis. Flowers contain reproductive structures and fruits hold seeds.
The document discusses how poor nutrition is linked to many health problems and deaths. It promotes nutritional products, claiming they can help control weight, hunger, energy levels and overall health by improving nutrient absorption and balancing blood sugar levels. The products are described as providing a balanced breakfast that is affordable, convenient and can lead to benefits like hunger control and stable energy within 30 days.
1. The document reports on developing a micropropagation protocol for Bacopa monnieri (Brahmi).
2. Various tissue culture techniques were tested, including callus culture and meristem culture, to optimize shoot initiation and proliferation using different concentrations of BAP and TDZ.
3. The results showed that MS medium supplemented with 2% BAP produced the highest number and length of shoots, making it the most effective treatment for shoot multiplication.
The document discusses photosynthesis and the key pigments and structures involved. It explains that:
- Photosynthesis occurs in the chloroplasts of plants, which contain chlorophyll, the pigment that absorbs light energy essential for photosynthesis.
- The two main stages of photosynthesis are the light-dependent reactions, where light energy is captured to make ATP and NADPH using water as the electron donor, and the light-independent reactions where CO2 is fixed into sugars using the ATP and NADPH produced.
- The main photosynthetic pigments are chlorophyll a and b as well as carotenoids, and each absorbs different wavelengths of light with peaks in the blue and red regions
This document summarizes an AP Biology lab review covering several topics:
- Lab 1 discusses diffusion and osmosis, describing experiments with dialysis tubing and potato cores in sucrose solutions. It concludes that water moves based on concentration gradients and molecule size.
- Lab 2 examines enzyme catalysis, measuring factors like pH and temperature that affect the rate of a reaction catalyzed by the enzyme catalase.
- Lab 3 covers mitosis and meiosis, describing experiments with onion root tips and fungi to observe cell stages and genetic recombination.
This document outlines a method for isolating genomic DNA from plant shoot tissue. The method involves grinding plant tissue, suspending it in extraction buffer, incubating, extracting with chloroform, precipitating the DNA with isopropyl alcohol, and re-suspending the DNA in buffer. On the second day, the isolated plant DNA is compared by gel electrophoresis to DNA isolated from chloroplasts using restriction enzyme digestion. The method allows isolation of nuclear, chloroplast, and mitochondrial DNA from plant cells. CTAB in the extraction buffer helps lyse cells and denature proteins while preserving DNA integrity for downstream applications.
The document discusses quality analysis in rice and focuses on micronutrient deficiencies. It notes that over 3 billion people worldwide suffer from malnutrition due to deficiencies in iron, zinc, vitamin A, and folic acid. Biofortification through traditional breeding or biotechnology can help increase micronutrients in rice to alleviate these deficiencies. The document then examines each deficiency in more detail and discusses the health problems they cause. It emphasizes the importance of analyzing rice quality and traits, including physical, milling, cooking, and nutritional properties, to address malnutrition issues.
This document provides an overview of algae. It describes that algae are thallophytes that contain chlorophyll as their primary photosynthetic pigment and lack a sterile cell covering around their reproductive cells. It classifies algae based on their habitat as aquatic, terrestrial, aerophytic, cryophytic, thermophytic, or of unusual habit. The document further discusses the structure of algal cells, types of algal thallus organization (unicellular, colonial, filamentous, dendroid), pigments, nutrition, reproduction (vegetative and sexual), examples of beneficial and harmful aspects, and references key characteristics of algae.
Biol2 Lecture 2 Dna Isolation And Agarose GelEricT1
The document discusses DNA isolation and agarose gel electrophoresis. It describes how DNA can be extracted from various biological sources and purified. The process involves lysing cells, removing proteins, and separating DNA from other molecules. Agarose gel electrophoresis is then used to separate DNA fragments by size, as smaller fragments move more quickly through the gel under an electric field. The document outlines the basic steps for preparing an agarose gel, loading samples mixed with dye, running the gel, and visualizing DNA bands under UV light.
Rice has been cultivated for over 9,000 years across Asia and is a staple food for over 5.6 billion people worldwide. There are several methods for cultivating rice, including transplanting seedlings, direct seeding of nursery beds, and dry/wet seeding. Proper crop establishment through nursery preparation and transplanting at the right time is crucial for maximizing yields. Rice cultivation requires adequate water management and fertilizer application to support the crop throughout its growth stages until ripening.
This document discusses postharvest handling of grains and cereals. It provides details on grading, treatment, packaging and marketing. It describes the different types of rice grains and their qualities. The key steps in postharvest treatment of rice include proper harvesting, drying, threshing, winnowing, storage and pest control techniques. Packaging of rice needs to protect the grain, be hygienic, convenient to handle and attract consumers. The document outlines national grading standards used in the Philippines for milled rice.
basic principles and protocol in plant tissue cultureAlia Najiha
Plant tissue culture involves growing plant cells, tissues, or organs in an artificial nutrient medium under sterile conditions. It allows for the rapid production of exact genetic copies of plants and is used to regenerate whole plants from genetically modified plant cells. The key requirements for successful tissue culture are appropriate explant tissue, a suitable growth medium, aseptic conditions to prevent microbial contamination, growth regulators, and frequent subculturing to provide nutrients and remove waste. The basic steps are selection of explant tissue from a healthy mother plant, sterilization of the tissue, establishment of the explant in a culture medium, multiplication through callus formation and shoot development, and root formation through manipulation of growth regulators. Benefits include providing pathogen-free plants
The presentation covered the basic steps of plant tissue culture, including setting up a tissue culture lab with areas for media preparation, aseptic transfer, culture, analysis, and acclimatization. It discussed media components and preparation, sterilization techniques like filtration, radiation, and chemicals, and sterilizing plant tissues using chemicals like sodium hypochlorite. Proper cleaning of glassware and sterilization of equipment and materials are essential for maintaining aseptic conditions.
The document discusses sterilization and autoclaves. It defines sterilization as removing microorganisms through processes like heat, chemicals, irradiation, or filtration. An autoclave is then described as a pressurized device that uses high temperature steam to achieve sterilization above water's boiling point. The document outlines the basic components, construction, and operating principles of autoclaves, including how increased pressure allows for higher sterilization temperatures. It also discusses autoclave usage, types, limitations, maintenance procedures, and quality control measures like indicators.
NAL Energy Corporation is an oil and gas producer focused on light oil with assets in western Canada. Some key points:
- Market cap of $1.2 billion with monthly dividend of $0.07/share and current yield of 10.4%
- Produces over 28,000 boe/day from assets in Alberta, southeast Saskatchewan, and British Columbia. Reserves of 104 MMBoe with 50% liquids.
- Focused on oil drilling for its 2011 $240 million capital program to maintain production of around 28,500 boe/day for the year. Hedges over 50% of oil volumes.
- Operates across different oil resource plays like the Cardium, V
Mage genes independent biomarker for prognosis 14-02-2012Cheng Luo
1. The document analyzes the correlation between methylation and gene expression of various MAGE genes.
2. Graphs show the relationships between methylation levels and gene expression levels for different MAGE genes.
3. Methylation is found to inhibit the expression of some MAGE genes, which could increase patient survival times.
Deutsche EuroShop is Germany's only public company that invests solely in shopping centers. It owns interests in 18 shopping centers located primarily in Germany, with a total lettable space of around 848,000 square meters. Deutsche EuroShop aims for long-term growth and stable increases in the value of its shopping center portfolio. Its key strategies include a "buy and hold" approach and targeting an annual portfolio extension of 10% primarily through acquisitions in Germany and Europe.
Preliminary participatory on-farm sorghum variety selection for tolerance to ...ILRI
Presented by S. Gudu, E.O. Ouma, A.O. Onkware, E.J. Too, B.A. Were, J.O. Ochuodho, C.O. Othieno, J.R. Okalebo, J. Agalo and S.M. Maina at the First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27 February 2013
The document provides highlights and financial results from Grupo Pão de Açúcar's (GPA) earnings release for fiscal year 2012.
- GPA saw 20.9% revenue growth to R$3,094.3 million in 2012, with same-store sales growth of 14.1%.
- Adjusted EBITDA increased 23% to R$191 million in 2012 with margins of 6.2%.
- GPA opened 96 new owned stores in 2012 and ended the year with 708 owned stores and 388 franchise stores.
- Capex was R$135 million in 2012 focused on organic growth, store renovations, and new distribution centers.
1. REEEP is an international NGO with over 200 partners across governments, businesses, and NGOs committed to accelerating renewable energy and energy efficiency.
2. REEEP is currently funded by 11 governments and the EU and aims to reduce market barriers for renewables and energy efficiency through 50 projects across 40 countries.
3. REEEP focuses on emerging markets and developing countries and believes action and technology transfer are improved through on the ground projects that target policy and innovative financing.
MLN Workshop: Breeding for maize lethal necrosis -- B DasCIMMYT
CIMMYT's maize breeding program focuses on developing varieties tolerant to biotic and abiotic stresses for tropical regions where maize is an important food crop. The program has screened over 45,000 varieties for tolerance to Maize Lethal Necrosis (MLN), finding that around 10% show some tolerance. Promising tolerant inbred lines and hybrids have been identified and some recommended for release. CIMMYT is expanding its MLN screening capacity through additional sites in East Africa that experience natural MLN infection. The goal is to accelerate the development and release of MLN-tolerant varieties to address the devastating impacts of the disease.
Botany is the study of plants. Key points from the document include:
- Plants are multicellular organisms that photosynthesize and produce their food from sunlight. They live on land and are anchored in place.
- Plant cells contain rigid cell walls, central vacuoles, and chloroplasts for photosynthesis.
- Plants are composed of tissues including dermal, ground, and vascular tissues which transport water and nutrients.
- The main plant organs are roots, stems, leaves, flowers, and fruits. Roots take in water and minerals while leaves perform photosynthesis. Flowers contain reproductive structures and fruits hold seeds.
The document discusses how poor nutrition is linked to many health problems and deaths. It promotes nutritional products, claiming they can help control weight, hunger, energy levels and overall health by improving nutrient absorption and balancing blood sugar levels. The products are described as providing a balanced breakfast that is affordable, convenient and can lead to benefits like hunger control and stable energy within 30 days.
1. The document reports on developing a micropropagation protocol for Bacopa monnieri (Brahmi).
2. Various tissue culture techniques were tested, including callus culture and meristem culture, to optimize shoot initiation and proliferation using different concentrations of BAP and TDZ.
3. The results showed that MS medium supplemented with 2% BAP produced the highest number and length of shoots, making it the most effective treatment for shoot multiplication.
The document discusses photosynthesis and the key pigments and structures involved. It explains that:
- Photosynthesis occurs in the chloroplasts of plants, which contain chlorophyll, the pigment that absorbs light energy essential for photosynthesis.
- The two main stages of photosynthesis are the light-dependent reactions, where light energy is captured to make ATP and NADPH using water as the electron donor, and the light-independent reactions where CO2 is fixed into sugars using the ATP and NADPH produced.
- The main photosynthetic pigments are chlorophyll a and b as well as carotenoids, and each absorbs different wavelengths of light with peaks in the blue and red regions
This document summarizes an AP Biology lab review covering several topics:
- Lab 1 discusses diffusion and osmosis, describing experiments with dialysis tubing and potato cores in sucrose solutions. It concludes that water moves based on concentration gradients and molecule size.
- Lab 2 examines enzyme catalysis, measuring factors like pH and temperature that affect the rate of a reaction catalyzed by the enzyme catalase.
- Lab 3 covers mitosis and meiosis, describing experiments with onion root tips and fungi to observe cell stages and genetic recombination.
This document outlines a method for isolating genomic DNA from plant shoot tissue. The method involves grinding plant tissue, suspending it in extraction buffer, incubating, extracting with chloroform, precipitating the DNA with isopropyl alcohol, and re-suspending the DNA in buffer. On the second day, the isolated plant DNA is compared by gel electrophoresis to DNA isolated from chloroplasts using restriction enzyme digestion. The method allows isolation of nuclear, chloroplast, and mitochondrial DNA from plant cells. CTAB in the extraction buffer helps lyse cells and denature proteins while preserving DNA integrity for downstream applications.
The document discusses quality analysis in rice and focuses on micronutrient deficiencies. It notes that over 3 billion people worldwide suffer from malnutrition due to deficiencies in iron, zinc, vitamin A, and folic acid. Biofortification through traditional breeding or biotechnology can help increase micronutrients in rice to alleviate these deficiencies. The document then examines each deficiency in more detail and discusses the health problems they cause. It emphasizes the importance of analyzing rice quality and traits, including physical, milling, cooking, and nutritional properties, to address malnutrition issues.
This document provides an overview of algae. It describes that algae are thallophytes that contain chlorophyll as their primary photosynthetic pigment and lack a sterile cell covering around their reproductive cells. It classifies algae based on their habitat as aquatic, terrestrial, aerophytic, cryophytic, thermophytic, or of unusual habit. The document further discusses the structure of algal cells, types of algal thallus organization (unicellular, colonial, filamentous, dendroid), pigments, nutrition, reproduction (vegetative and sexual), examples of beneficial and harmful aspects, and references key characteristics of algae.
Biol2 Lecture 2 Dna Isolation And Agarose GelEricT1
The document discusses DNA isolation and agarose gel electrophoresis. It describes how DNA can be extracted from various biological sources and purified. The process involves lysing cells, removing proteins, and separating DNA from other molecules. Agarose gel electrophoresis is then used to separate DNA fragments by size, as smaller fragments move more quickly through the gel under an electric field. The document outlines the basic steps for preparing an agarose gel, loading samples mixed with dye, running the gel, and visualizing DNA bands under UV light.
Rice has been cultivated for over 9,000 years across Asia and is a staple food for over 5.6 billion people worldwide. There are several methods for cultivating rice, including transplanting seedlings, direct seeding of nursery beds, and dry/wet seeding. Proper crop establishment through nursery preparation and transplanting at the right time is crucial for maximizing yields. Rice cultivation requires adequate water management and fertilizer application to support the crop throughout its growth stages until ripening.
This document discusses postharvest handling of grains and cereals. It provides details on grading, treatment, packaging and marketing. It describes the different types of rice grains and their qualities. The key steps in postharvest treatment of rice include proper harvesting, drying, threshing, winnowing, storage and pest control techniques. Packaging of rice needs to protect the grain, be hygienic, convenient to handle and attract consumers. The document outlines national grading standards used in the Philippines for milled rice.
basic principles and protocol in plant tissue cultureAlia Najiha
Plant tissue culture involves growing plant cells, tissues, or organs in an artificial nutrient medium under sterile conditions. It allows for the rapid production of exact genetic copies of plants and is used to regenerate whole plants from genetically modified plant cells. The key requirements for successful tissue culture are appropriate explant tissue, a suitable growth medium, aseptic conditions to prevent microbial contamination, growth regulators, and frequent subculturing to provide nutrients and remove waste. The basic steps are selection of explant tissue from a healthy mother plant, sterilization of the tissue, establishment of the explant in a culture medium, multiplication through callus formation and shoot development, and root formation through manipulation of growth regulators. Benefits include providing pathogen-free plants
The presentation covered the basic steps of plant tissue culture, including setting up a tissue culture lab with areas for media preparation, aseptic transfer, culture, analysis, and acclimatization. It discussed media components and preparation, sterilization techniques like filtration, radiation, and chemicals, and sterilizing plant tissues using chemicals like sodium hypochlorite. Proper cleaning of glassware and sterilization of equipment and materials are essential for maintaining aseptic conditions.
The document discusses sterilization and autoclaves. It defines sterilization as removing microorganisms through processes like heat, chemicals, irradiation, or filtration. An autoclave is then described as a pressurized device that uses high temperature steam to achieve sterilization above water's boiling point. The document outlines the basic components, construction, and operating principles of autoclaves, including how increased pressure allows for higher sterilization temperatures. It also discusses autoclave usage, types, limitations, maintenance procedures, and quality control measures like indicators.
Callus is an unorganized mass of undifferentiated cells that can be cultured in vitro. It is produced when plant explants are cultured on medium containing auxin and cytokinin hormones under sterile conditions. Callus tissue lacks differentiation and is unable to perform photosynthesis. It can be maintained indefinitely and used for plant regeneration through processes like organogenesis and somatic embryogenesis. Successful callus culture requires aseptic preparation of explants, a nutrient medium with proper hormone balance, and controlled physical conditions for incubation.
This document contains protocols for various plant tissue culture techniques. It discusses the introduction to plant tissue culture, sterilization techniques used, and then outlines 8 specific protocols: 1) tissue culture media preparation, 2) explant preparation and surface sterilization, 3) embryo culture, 4) culture of anther for haploid production, 5) meristem culture, 6) meristem tip culture for virus-free plants, 7) induction of somatic embryogenesis, and 8) protoplast isolation, culture, and regeneration. The goal of these protocols is to describe the principles and procedures of different plant tissue culture methods.
Tissue culture is a technique where cells, tissues or whole plants are grown in a sterile nutrient culture medium under controlled conditions. It allows for rapid vegetative propagation of plants. Key steps include sterilizing equipment and explants, preparing nutrient medium, subculturing to promote growth, and rooting and hardening plantlets. Tissue culture has many applications like mass multiplication of crops and plants, eliminating diseases, and genetic modification. It is used commercially for propagating crops but contamination and rooting difficulties can be issues.
The document discusses David Cameron's vision of the "Big Society" which aims to transfer power from the state to citizens and communities by breaking apart the old system. It also discusses the UK's deficit reduction priorities, including plans to eliminate the structural deficit through £40 billion in annual spending reductions by 2014-2015. Charts show trends in UK GDP growth, house prices, and total government expenditure between 1997-2014.
This keynote paper discusses strategies for stabilizing food prices in Bangladesh. It begins by providing context on food security and the components of a balanced diet. It then examines trends in foodgrain and commodity production, noting impressive growth in rice but stagnation in other crops. Challenges exacerbating food price volatility are explored, including population growth, poverty, and environmental factors. Unlocking potentials for price stabilization is discussed, emphasizing self-sufficiency through increased productivity, research, protecting agricultural land, and diversifying food consumption patterns. The conclusion stresses the importance of enhancing investment in agriculture and maintaining adequate public food stocks to curb market manipulation and ensure affordability.
The document summarizes the past races and pedigree of racehorse Master of Hounds, who is competing in the 143rd running of the Belmont Stakes on June 11, 2011. Master of Hounds is a 3-year old colt sired by Kingmambo out of Silk And Scarlet. His past performances include wins in maiden races in Ireland and a fourth place finish in the 2010 Breeders' Cup Juvenile Turf. He is the 10-1 morning line odds choice for the Belmont Stakes with a post position of 1 and will be ridden by jockey G K Gomez.
- Net revenue increased 29% from 1Q11 to 2Q11, while gross profit increased over 300% and EBITDA improved from a loss to a small profit. However, net losses expanded due to a dividend payment.
- The company completed R$140 million of residential projects in 2Q11, selling 88-98% of delivered units.
- Cash decreased in the quarter due to swap rights acquisition and debt payments, though receivables total R$402 million and debt is concentrated in 2011-2012.
- The land bank contains projects in São Paulo and Rio de Janeiro states with a total potential sales value (PSV) of R$2.5 billion and estimated cash recovery of
- Net revenue increased 29% from 1Q11 to 2Q11, while gross profit increased over 300% and EBITDA improved from a loss to a small profit. However, net losses expanded due to a dividend payment.
- The company completed R$140 million of residential projects in 2Q11, selling 88-98% of available units.
- Cash decreased in 2Q11 primarily due to payments for swap rights for the Cidade Paradiso project and amortization of debt. Receivables remain over R$400 million while debt will be paid down over the next few years.
- The company has several future residential projects in its land bank concentrated in Rio de Janeiro and Sao
The document discusses Tomahawk motorcycle defect conditions found per 100 motorcycles from January 2008 to December 2008. It shows that:
1) Additional inspections in February 2008 found approximately 40% more buffable defects like dirt on fairings and covers.
2) In July 2008, two additional inspectors were added to find more obvious issues and cosmetic defects.
3) By October 2008, the number of inspectors went back down to one to focus on just obvious defects.
The Belmont Stakes is a Grade I stakes race for three-year-olds held at Belmont Park on June 5, 2010. The purse is $1,000,000 with 60% to the winner, 20% to second place, 11% to third place, 6% to fourth place, and 3% to fifth place. Colts and geldings carry 126 pounds while fillies carry 121 pounds. The field includes Dave in Dixie as the 20-1 morning line favorite.
Weather Outlook - Dr. Elwynn Taylor, Climatologist, Ag Meteorologist, Iowa State University, from the 2012 World Pork Expo, June 6-8, Des Moines, Iowa, USA.
Workspace analysis of stewart platformMarzieh Nabi
The document describes research to increase the workspace of a Stewart platform using an optimized movable base. It introduces Stewart platforms and their applications. It then discusses limitations of workspace due to actuator length and joint constraints. Two questions are posed regarding platform architecture and guaranteeing continuous motion. Solutions explored include a hybrid Stewart platform with a movable middle plate, optimization of platform geometry using genetic algorithms and adaptive simulated annealing, and inverse kinematics analysis. The optimized hybrid platform is shown to increase workspace coverage compared to a standard Stewart platform.
The document is a race card for the Kentucky Derby at Churchill Downs on May 1, 2010. It lists the post positions and odds for the horses running in the race. Lookin At Lucky is the morning line favorite at 3-1 odds. Noble's Promise has odds of 5-1 and Ice Box is listed at 20-1 odds.
This document provides a template and process for conducting a root cause analysis investigation. The investigation process involves: 1) defining the problem, 2) mapping the process, 3) gathering data, 4) conducting a cause/effect analysis to identify the root cause, 5) verifying the root cause with data, 6) developing solutions and prevention steps, 7) piloting implementations, 8) full implementation, and 9) establishing a control/monitoring plan with process metrics and lessons learned. The template guides investigators through each step and includes sections for process mapping, cause/effect diagrams, recommended solutions, and an action plan for implementation.
T2 Partners Presentation On The Housing Crisis 11 9 09mattd22556
The housing crisis is evolving in several ways:
1) Defaults are shifting from subprime loans to prime and Alt-A loans.
2) Defaults are now driven more by job losses and falling home prices than adjustable rate resets.
3) Foreclosures are impacting many more states beyond the original "sand and rust states".
4) Future losses will come from banks' on-balance sheet loans rather than losses from securitized loans marked to market.
Corey Bradshaw_Assessing bias in extinction predictions from species-area rel...TERN Australia
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3. Farming Systems
South Region
Central Region
75% of Brazilian production
25% of Brazilian production
4.
5. Rice production in Brazil and RS in
the last two seasons
Seasons Variation
Cultivated area (ha)
2010/11 2011/12 (%)
Brazil 2820300 2562700 - 9.1
RS 1171600 1053000 -10.1
RS / Brazil (%) 41,5 41,1
Total production (paddy, ton.)
Brazil 13613100 11170800 -20.9
RS 8904200 7042500 -17.9
RS / Brazil (%) 65,4 63,0
6. Rice at Rio Grande do Sul State
Season 2010/11 = 8.904 millions MT
8 1,5
Paddy yield (t/ha) 1,4
Planted area (Million ha
y = 5,361 + 0,208 x El Niño
7
1,3
Paddy yield (t/ha)
1,2
6
El Niño
1,1
5 1,0
Planted area (million ha) 0,9
4 y = 0,940 + 0,01729 x
0,8
0 0,0
1
2
3
4
5
6
7
8
9
0
1
-0
-0
-0
-0
-0
-0
-0
-0
-0
-1
-1
00
01
02
03
04
05
06
07
08
09
10
20
20
20
20
20
20
20
20
20
20
20
Crop season
12. CLEARFIELD IS VERY IMPORTANT FOR RICE
CULTIVATION IN SOUTHERN BRAZIL
BEFORE CLEARFIELD TECHNOLOGY AFTER CLEARFIELD TECHNOLOGY
YILED YIELD
4.000 kg/ha 8.000 kg/ha
14. Cultivated area (%)
0
5
10
15
20
25
30
35
40
45
50
PU
ITA
INT
A-C
L
IRG
A4
24
Ep
agr
i 's
IRG
A4
17
BR
- IR
GA
409
Área cultivada (%)
0
10
20
30
40
50
60
70
INI
AO
lim Ear
ar ly
63,2
Cultivars
Hy Me
diu
bri
ds m
27,3
a) Maturity group
BR
SS
inu Lat
e
9,6
elo
CL
IRG
A4 Área cultivada (%)
0
10
20
30
40
50
22C
L
Season 2011/12
BR
SQ
Rice varieties in RS
uer CL
ê
54,2
b–v
J
BR
S7
Tai
m Co
nv .
45,8
b) CLEARFIELD cultivars
El P
ass
o 144
L
15. IRGA
Research Division
Cachoeirinha, RS - Brazil
16. Field Day at Rice
Experiment Station
2012, 08 February
18. Rice Breeding
General Program Hybrid Program
Adaptation Development
to cold Development of hybrid herbicide
Herbicides Abiotic Stresses Diseases regions of tolerant cultivars
of hybrid cultivars
Tolerance tolerance Resistance RS
Development of
Hybrid rice
parental lines (P.
evaluations
Development of Recurrent selection L.)
segregant populations breeding
Seeds production
Mutation Submersion and salinity of P. L. and hybrid
tolerance breeding cultivars
Yield and quality
grain evaluations Development of P.
L. trought
recurrent
selection
19. Breeding Partners:
• FLAR - Temperate, Tropical and RD’s (durable
resistance to blast)
• Rice Institute of Hunan and Ana Paula -
Hybrids
• EMBRAPA – Recurrent Selection
• CENA - Mutation
• FURG – Grain contaminants
• FEPAGRO – Yield trials
• BASF - CL
• BAYER - Hybrids
21. General Program
• Objective: Rice cultivars development to different
regions of RS State.
• Actions: Crossings, selections, yield trials,
evaluations of diseases, iron toxicity and grains
quality, mutations, winter generation in NE
(Penedo, AL)
• Subprojects: Pre-germinated (water seeding
system), FLAR, Recurrent Selection
22.
23. Rice yield trials
Uruguaiana
Cachoeira do Sul
Dom Pedrito
Santa Vitória do Palmar
Camaquã
30. IRGA 425
• First variety for pre-germinated system
• Iron tolerant and medium resistant to blast
• High grain quality
31. New
Cultivar
IRGA 427
High yielding, good grain quality and
adaptation to warmer regions
Average yield = 10,1 t/ha
High yielding
Excellent grain quality
32. Rice breeding trough recurrent selection
1- Population Pirga 1/0/Pr/2 : Conducted in substation of
Uruguaiana, for extracting lines with high yield potential;
2- Population Pirga 2/0/Fr/2 : Conducted in substation of Stª Vitória
do Palmar, for extractiong lines with high adaptation to cold
region (south) of RS;
3- Population Pirga 3/0/Py/0 : Conducted at Cachoeirinha and
Torres for selecting lines with resistance to blast (Pyricularia
grisea).
AGREEMENT IRGA/EMBRAPA:
EMBRAPA: CNA 11 e CNA 12;
IRGA: Pirga 1/0/Pr/2 e Pirga
2/0/Fr/2.
34. Breeding line:
1. Cultivar development with resistance to blast
(Pyricularia grisea) trough “Hot spot” method and
gene acumulation.
Researchers: Gustavo Daltrozo Funck e Dieter Kempf
Achievements: released cultivars with resistance to blast:
• RESISTENT: IRGA 423, IRGA 424 and IRGA 426
• MR / MS: IRGA 425, IRGA 427 and IRGA 428
Goals:
1. Evaluate annually around 5000 genotypes;
2. Accumulate three genes which confer resistance to
blast: Pi 1, Pi 2 and Pi 3;
3. Evaluate the incidence of major leaf spots and grain
yield in the tests (VCU, Preliminary and Advanced).
37. LABORATORY of PLANT PATHOLOGY
Diagnosis of plant diseases and seed health analysis
38. Cultivars development with
herbicide tolerance
1- Developing cultivars for the Clearfield System
2 - Induced mutation for the development of
cultivars tolerant to herbicides
39. Cultivars development with
herbicide tolerance
• 1.1- Conversion of cultivar IRGA 420:
Released in 2011
• 1.2- Conversion of cultivar IRGA 424: It will be
released in 2013
• 2.1- Screening for tolerance to Imidazolinone
herbicides – gene sequencing test
• 2.2- New mutants: Imidazolinones and Oxine
Ciclohexanodione
41. New
Cultivar
IRGA 428
High yielding, good plant, smooth leaves,
tolerant to excess of iron in the soil, and
second generation for herbicide tolerance
Average yield = 9,8 t/ha
Yield trial
Good grain quality
42. RESEARCH LINE: COLD TOLERANCE
Goal: Develop cultivars suited to colder areas of the RS
and with cold tolerance in early stages.
Achievements: cultivars released:
RGA 424 - 2007
IRGA 426 - 2011
43. IRGA 424
• High yielding variety
• Iron tolerant and blast resistant
• Adaptation to south region
44. New
Cultivar
IRGA 426
Adaptation, high yielding and good quality to
cold regions of RS
Yield = 10,0 t/ha
Great panicles High grain quality
45. Research line – Molecular Markers Assisted
Selection
Researcher - Dra. Danielle Costenaro da Silva
Objetivos:
1- MAS as a tool for conversion of cultivar IRGA 424 for the Clearfield
production system.
2- MAS for blast genes Pi-1, Pi-2 e Pi-33;
Results obtained:
1- 89 primers – 18 % polymorphic (Puitá INTA CL e IRGA 424)
2- 8 primers studied:
RM224 – Pi-1
RM404 – Pi-33
RM72 – Pi-33
RM527 – Pi-2
48. Hybrid Rice Program
Goal:
Develop hybrid rice cultivars, adapted to the conditions of the RS,
establishing a system of mechanized production of hybrid seeds
Focus:
Increase the yield potential– 20 %
Tool for introduction of new technologies:
Clearfield
etc...
49. Hybrid Rice Program
Activities:
Cachoeirinha, RS
Parental lines development (A, B e R)
Hybrids evaluation
Seeds production of improved hybrids
Penedo, AL (NE)
Parental lines development (A, B e R)
Crossings
Seeds production of improved hybrids for trials
50. New hybrid cultivar: QM 1010 (H9)
IRGA / ANA PAULA / BAYER
Rice Experiment Station – Cachoeirinha - 2007/2008
Yield - 11.540 kg/ha
Grains per panicle - 197
Sterility - 9,7 %
51. Grain quality
Visual appearance of grain quality of Hybrid 9 compared to IRGA 417.
Híbrido 9 Irga 417
52. New Hybrids:
• Released:
– QM 1010 and IRGAP H7
– Herbicide Resistant: IRGA H7 RI and IRGAP H9 RI
• Improved lines:
– 13 hybrids: yield trial – VCU
– 10 CL hybrids: yield trial - VCU
55. Major problems for rice breeding
• Red rice control
• Yield potential x grain quality
• Lodging resistance
• Seedling vigor
• Diseases: blast, leaf spots, stem and sheath
rots
• Iron toxicity resistance
• Cold tolerance to south regions
56. Rice Researchers at Breeding Staff
1. Antonio Folgiarini de Rosso – Breeder - Hyb.
2. Mara Cristina Barbosa Lopes – Breeder
3. Oneides Antonio Avozani – Breeder
4. Sergio Iraçu Gindri Lopes – Breeder
5. Gustavo Daltrozo Funck - Pathologist
6. Danielle Costenaro – Biotech.
7. Ana Paula Fontini – Breeder – Hyb.
8. Daniel Dawlow – Breeder – Hyb. New
Researchers
9. Gabriela Magalhães – Breeder
10.Roberto Weiler – Breeder
57. Thank you!
Sérgio Iraçu Gindri Lopes
Mara Cristina Barbosa Lopes
IRGA - Brazil
Foto: Ereno, março 2010